##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934128.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	839963
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.869628781267746	31.0	31.0	34.0	30.0	34.0
2	32.06339922115617	33.0	31.0	34.0	30.0	34.0
3	32.138401334344486	33.0	31.0	34.0	30.0	34.0
4	35.63790190758402	37.0	35.0	37.0	33.0	37.0
5	29.551612392450618	37.0	35.0	37.0	0.0	37.0
6	23.668665167394277	35.0	10.0	37.0	0.0	37.0
7	30.376716593469	35.0	19.0	37.0	17.0	37.0
8	34.26864516651329	35.0	32.0	37.0	32.0	37.0
9	36.903262405605965	38.0	37.0	39.0	34.0	39.0
10	36.8040235105594	38.0	37.0	39.0	33.0	39.0
11	36.983783809524944	39.0	37.0	39.0	33.0	39.0
12	37.09789240716555	39.0	37.0	39.0	34.0	39.0
13	37.109695308007616	39.0	37.0	39.0	33.0	39.0
14	38.2317494937277	40.0	38.0	41.0	34.0	41.0
15	38.08661691050677	40.0	37.0	41.0	33.0	41.0
16	38.282466013383925	40.0	38.0	41.0	34.0	41.0
17	38.28702573803846	40.0	37.0	41.0	34.0	41.0
18	38.367573333587316	40.0	38.0	41.0	34.0	41.0
19	38.37440458686871	40.0	38.0	41.0	34.0	41.0
20	38.3339397092491	40.0	38.0	41.0	34.0	41.0
21	38.234172219490624	40.0	37.0	41.0	34.0	41.0
22	38.293020049692664	40.0	37.0	41.0	34.0	41.0
23	38.2375402249861	40.0	37.0	41.0	34.0	41.0
24	38.21207362705262	40.0	37.0	41.0	34.0	41.0
25	37.95518969287933	40.0	37.0	41.0	33.0	41.0
26	37.87091931430313	40.0	37.0	41.0	33.0	41.0
27	37.05319043815025	40.0	37.0	41.0	32.0	41.0
28	37.4343929434987	40.0	37.0	41.0	32.0	41.0
29	37.70407029833457	40.0	37.0	41.0	32.0	41.0
30	37.69292337876787	40.0	36.0	41.0	33.0	41.0
31	37.73902540945256	40.0	37.0	41.0	33.0	41.0
32	37.7431351142848	40.0	37.0	41.0	33.0	41.0
33	36.62168571710897	40.0	36.0	41.0	31.0	41.0
34	37.132680844275285	40.0	36.0	41.0	31.0	41.0
35	37.42560327062025	40.0	36.0	41.0	32.0	41.0
36	37.545554982779	40.0	36.0	41.0	33.0	41.0
37	37.64023058158514	40.0	37.0	41.0	33.0	41.0
38	37.51868713264751	40.0	36.0	41.0	33.0	41.0
39	37.46375257005368	39.0	36.0	41.0	33.0	41.0
40	37.47549237287833	39.0	36.0	41.0	33.0	41.0
41	37.50389124282855	40.0	36.0	41.0	33.0	41.0
42	37.56690830429436	40.0	36.0	41.0	33.0	41.0
43	37.429584398360404	40.0	36.0	41.0	33.0	41.0
44	37.424653228773174	39.0	36.0	41.0	32.0	41.0
45	37.3511749922318	39.0	36.0	41.0	32.0	41.0
46	37.28916988010186	39.0	36.0	41.0	32.0	41.0
47	37.23612944855904	39.0	36.0	41.0	32.0	41.0
48	37.15681166908542	39.0	35.0	40.0	32.0	41.0
49	37.12221014497067	39.0	35.0	40.0	32.0	41.0
50	36.93501261365084	39.0	35.0	40.0	32.0	41.0
51	36.07430446341089	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	10.0
11	6.0
12	6.0
13	12.0
14	5.0
15	16.0
16	31.0
17	36.0
18	65.0
19	163.0
20	319.0
21	529.0
22	919.0
23	1428.0
24	2068.0
25	2985.0
26	3899.0
27	4943.0
28	6431.0
29	9031.0
30	12517.0
31	17932.0
32	25709.0
33	39427.0
34	55612.0
35	75007.0
36	102892.0
37	158723.0
38	177602.0
39	141565.0
40	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.42067448209028	4.391026747606739	57.964696063993294	14.223602706309682
2	19.984570748949658	5.523576633732676	53.362707643074756	21.129144974242912
3	19.476215023757	5.201062427749794	52.74494233674578	22.577780211747424
4	18.22306458736873	4.259354281081428	52.93137912027077	24.586202011279067
5	14.784698849830292	21.87667790128851	43.2954784913145	20.043144757566704
6	15.422465037150445	29.947152434095308	41.29169975344152	13.338682775312721
7	81.82360413494403	1.878654178815019	11.292045006744345	5.005696679496597
8	83.48510589156903	2.604162326197702	7.859512859495002	6.051218922738263
9	78.35190359575363	4.631870689542277	10.4464125205515	6.569813194152599
10	36.94388919511931	26.307706410877625	20.872109842933558	15.876294551069511
11	26.582837577369478	26.541169075304506	25.590174805318807	21.285818542007206
12	21.537734400205725	22.57254188577354	33.809822575518204	22.079901138502528
13	22.300982305172965	24.90478747278154	33.77541629809884	19.018813923946652
14	20.114933633981497	27.853012573172865	31.60627313345945	20.425780659386188
15	18.64665467407493	26.50735806220036	33.9336375530827	20.912349710642015
16	18.859164034606284	25.60827084050131	31.54317511604678	23.98939000884563
17	19.02988584020963	26.582242313054266	29.731071487672672	24.656800359063435
18	20.122433964353192	25.22087282416011	32.02522015850698	22.631473052979715
19	20.874967111646583	27.486805966453282	29.436653757367885	22.20157316453225
20	22.712428999848804	27.423231737588445	29.949057279903997	19.91528198265876
21	23.152567434517948	27.470852882805552	29.149498251708707	20.227081430967793
22	20.882705547744365	25.813160817797925	28.40589406914352	24.89823956531419
23	20.176960175626785	27.305131297450007	27.609311362524302	24.90859716439891
24	21.687383849050494	25.69934628072903	28.653881182861625	23.95938868735885
25	19.811705991811543	27.965755634474377	27.009046827062622	25.21349154665146
26	20.360896849027874	27.578119512407095	28.20695673499904	23.854026903565988
27	20.98330521701551	28.68745409023969	27.588000900039646	22.741239792705155
28	19.718130441459923	26.03150376861838	30.56848932631556	23.681876463606137
29	20.226009955200407	23.932601793174225	30.9337435101308	24.90764474149457
30	21.843462152499573	26.52878757754806	28.801863891623796	22.82588637832857
31	21.660001690550658	27.820868300151314	27.90372909282909	22.61540091646894
32	21.195100260368612	28.156716426794993	28.517446601814605	22.130736711021797
33	20.892110723924745	28.53197105110582	26.957854095954225	23.618064129015206
34	21.571188254720745	25.205991216279767	29.49296576158712	23.729854767412373
35	21.097357859810494	23.556275693096005	29.82548040806559	25.520886039027907
36	24.606202892270254	25.19730035727764	27.221794293320063	22.97470245713204
37	21.257245854876942	25.050031965693726	30.882907937611538	22.809814241817794
38	20.76198594461899	26.827372158059344	30.230617301000162	22.180024596321505
39	21.179028123857837	26.499738678965624	31.817115753908208	20.504117443268335
40	21.440944422551947	24.90145399261634	32.10415220670434	21.55344937812737
41	21.13069266146247	24.046059171653987	29.75166763297907	25.07158053390447
42	23.518893094100573	22.894817986030336	29.196286026884515	24.390002892984572
43	24.38345498551722	22.89446082744121	28.460063121827982	24.262021065213588
44	21.669168761004947	23.20411732421547	28.676620279702796	26.450093635076783
45	22.108592878495838	22.840172721893705	27.737293190295286	27.313941209315175
46	23.237809284456574	23.37210091396883	29.711784923859742	23.67830487771485
47	19.791347952231227	24.02212954618239	32.621555949488254	23.56496655209813
48	20.76960532785373	22.64837855953179	31.530674565427287	25.0513415471872
49	22.645878449407892	21.32498693394828	32.73346564074846	23.295668975895367
50	22.385509837933338	20.677458411858616	30.90136113138317	26.035670618824874
51	20.125886497381433	20.96747118623082	29.212120057669207	29.694522258718536
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	110.0
1	262.0
2	414.0
3	966.5
4	1519.0
5	1149.0
6	779.0
7	841.0
8	903.0
9	1089.0
10	1275.0
11	1449.5
12	1624.0
13	1640.5
14	1657.0
15	1740.0
16	1823.0
17	1900.5
18	1978.0
19	1998.0
20	2018.0
21	2036.0
22	2054.0
23	2515.0
24	2976.0
25	3739.0
26	5265.5
27	6029.0
28	7546.5
29	9064.0
30	10738.5
31	12413.0
32	14495.5
33	16578.0
34	19657.0
35	22736.0
36	25256.5
37	27777.0
38	30686.5
39	33596.0
40	38147.5
41	42699.0
42	49539.0
43	56379.0
44	66159.0
45	75939.0
46	90595.5
47	105252.0
48	111703.5
49	118155.0
50	107928.0
51	97701.0
52	79970.5
53	62240.0
54	51277.0
55	40314.0
56	34502.5
57	28691.0
58	24944.0
59	21197.0
60	18172.5
61	15148.0
62	12199.0
63	9250.0
64	7684.0
65	6118.0
66	4967.0
67	3816.0
68	3082.0
69	2348.0
70	1783.0
71	1218.0
72	997.5
73	777.0
74	616.0
75	384.0
76	313.0
77	179.0
78	45.0
79	45.5
80	46.0
81	35.5
82	25.0
83	17.5
84	10.0
85	6.0
86	2.0
87	1.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	839963.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.31943486493732
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.32353781698416	31.76334220529614
2	11.684333056357628	10.123174095502366
3	4.392753571161926	5.708748066110096
4	2.3687312849540625	4.104484024444272
5	1.5202612016497659	3.2928428051279184
6	1.1025487214527614	2.8657072514635695
7	0.8411932080123504	2.550801006832308
8	0.6582760526973112	2.2812917270375377
9	0.5307441427154734	2.0692382688278954
>10	3.4310305282912346	25.413148180271815
>50	0.09258352493316391	2.7518845973152386
>100	0.04743462365718183	3.5018948452629455
>500	0.0034290089389549423	0.9283920586396655
>1k	0.0031432581940420306	2.6450508678683162
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	3690	0.43930506462784674	TruSeq Adapter, Index 22 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGCT	3255	0.3875170692042388	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	2846	0.33882444821974306	TruSeq Adapter, Index 22 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	2255	0.2684642061614619	TruSeq Adapter, Index 22 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGC	1771	0.21084262044875787	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCG	1582	0.18834162933367302	No Hit
CCTGTATCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	1376	0.163816739546861	TruSeq Adapter, Index 20 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTC	1241	0.1477446030360861	No Hit
TCTGTATCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGC	1209	0.14393491141871725	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCACTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTGCT	1101	0.13107720221009736	TruSeq Adapter, Index 20 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCGCGTATTGTCGTATGCCGTCTTCTG	1042	0.12405308329057352	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.1905286304277689E-4	0.0	0.0	0.4132324876214786	0.0
2	1.1905286304277689E-4	0.0	0.0	1.5622116688473182	0.0
3	1.1905286304277689E-4	0.0	0.0	2.194025213015335	0.0
4	1.1905286304277689E-4	0.0	0.0	2.8873890873764676	0.0
5	1.1905286304277689E-4	0.0	0.0	4.073393709008611	0.0
6	1.1905286304277689E-4	0.0	0.0	4.875214741601713	0.0
7	1.1905286304277689E-4	0.0	0.0	5.886568813150103	0.0
8	1.1905286304277689E-4	0.0	0.0	7.527950635920868	0.0
9	1.1905286304277689E-4	0.0	0.0	8.16464534747364	0.0
10	1.1905286304277689E-4	0.0	0.0	9.764001509590303	0.0
11	1.1905286304277689E-4	0.0	0.0	12.486740487378611	0.0
12	1.1905286304277689E-4	0.0	0.0	15.066139818063414	0.0
13	1.1905286304277689E-4	0.0	0.0	15.82903056444153	0.0
14	1.1905286304277689E-4	0.0	0.0	16.067731554842297	0.0
15	1.1905286304277689E-4	0.0	0.0	16.460367897157376	0.0
16	1.1905286304277689E-4	0.0	0.0	17.441363488629857	0.0
17	1.1905286304277689E-4	0.0	0.0	18.880593549953986	0.0
18	1.1905286304277689E-4	0.0	0.0	20.466377685683774	0.0
19	1.1905286304277689E-4	0.0	0.0	21.606070743592277	0.0
20	1.1905286304277689E-4	0.0	0.0	22.65325972691654	0.0
21	1.1905286304277689E-4	0.0	0.0	24.122253004001365	0.0
22	1.1905286304277689E-4	0.0	0.0	25.696369959152964	0.0
23	1.1905286304277689E-4	0.0	0.0	27.258700680863324	0.0
24	2.3810572608555378E-4	0.0	0.0	28.414465875282602	0.0
25	2.3810572608555378E-4	0.0	0.0	29.40903349314196	0.0
26	2.3810572608555378E-4	0.0	0.0	30.27978613343683	0.0
27	2.3810572608555378E-4	0.0	0.0	31.1282758883427	0.0
28	2.3810572608555378E-4	0.0	0.0	32.03164901311129	0.0
29	2.3810572608555378E-4	0.0	0.0	32.957880287584096	0.0
30	2.3810572608555378E-4	0.0	0.0	33.978639535312865	0.0
31	2.3810572608555378E-4	0.0	0.0	34.914633144555175	0.0
32	2.3810572608555378E-4	0.0	0.0	35.84395979346709	0.0
33	2.3810572608555378E-4	0.0	0.0	36.711378953596764	0.0
34	2.3810572608555378E-4	0.0	0.0	37.596656043182854	0.0
35	2.3810572608555378E-4	0.0	0.0	38.501814960897086	0.0
36	2.3810572608555378E-4	0.0	0.0	39.40661672002219	0.0
37	2.3810572608555378E-4	0.0	0.0	40.28153621052356	0.0
38	2.3810572608555378E-4	0.0	0.0	41.16169402699881	0.0
39	2.3810572608555378E-4	0.0	0.0	42.01875558804376	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGGG	20	7.0322E-4	45.000004	3
TCCGCAT	30	2.1648011E-6	45.000004	12
CTTCCCG	20	7.0322E-4	45.000004	1
ACGCAAT	30	2.1648011E-6	45.000004	18
GGCGTAG	45	3.8380676E-10	45.000004	1
GACCGAA	90	0.0	45.000004	17
TTCGTCG	30	2.1648011E-6	45.000004	1
CAATTCG	20	7.0322E-4	45.000004	16
CGTTATC	20	7.0322E-4	45.000004	21
ATCCGGC	45	3.8380676E-10	45.000004	6
ATCCGCA	20	7.0322E-4	45.000004	11
TACGACA	20	7.0322E-4	45.000004	45
ACTACGC	20	7.0322E-4	45.000004	15
TTGCGAG	45	3.8380676E-10	45.000004	1
GCGATAG	20	7.0322E-4	45.000004	9
CGCAATC	30	2.1648011E-6	45.000004	19
GCGTAAA	20	7.0322E-4	45.000004	38
GCCCGCG	35	1.2114106E-7	45.000004	14
CGGTACG	55	1.8189894E-12	45.0	3
TATACGA	25	3.8899052E-5	45.0	43
>>END_MODULE