FastQCFastQC Report
Sat 14 Jan 2017
SRR2934127.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934127.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1050625
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC39620.37710886377156455No Hit
CTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT34870.33189767995240926No Hit
TCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC25910.2466151100535396No Hit
GCTGTCTCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC20250.19274241522903035No Hit
CCTGTATCTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGC13900.1323022010707912No Hit
CTGTCACTTATACACATCTGACGCCTTGGTCATCGTATGCCGTCTTCTGCT12510.11907198096371208No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTC207.0330093E-445.037
TAGCGAG207.0330093E-445.01
GCACGTC253.8905764E-545.025
CGTCGTT207.0330093E-445.030
CCGAATT207.0330093E-445.024
TATCGGA207.0330093E-445.014
TTCGACA207.0330093E-445.015
TCGAAAC253.8905764E-545.012
CGAGGGA2350.043.0851064
TCTTACG1750.042.428571
GGCGATA750.042.0000048
CTCGTAC1050.040.71428729
TACGGGA5750.040.6956524
TTCGTAG1400.040.1785741
TAGGGCG5450.040.045875
TTAGGGA20600.039.9757274
CGTTAGG2200.039.8863642
GCTCTAG2100.039.6428571
TGTACGA800.039.37515
GTACGAT800.039.37516
Produced by FastQC (version 0.11.5)