##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934126.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	387387
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.88497548962665	33.0	31.0	34.0	30.0	34.0
2	32.11126341358899	33.0	31.0	34.0	30.0	34.0
3	32.180653971351646	34.0	31.0	34.0	30.0	34.0
4	35.63226953924628	37.0	35.0	37.0	33.0	37.0
5	29.52793717909997	37.0	35.0	37.0	0.0	37.0
6	23.610301842859982	35.0	10.0	37.0	0.0	37.0
7	30.332713797830078	35.0	19.0	37.0	17.0	37.0
8	34.23374558258279	35.0	32.0	37.0	32.0	37.0
9	36.84387447178145	38.0	37.0	39.0	34.0	39.0
10	36.95567739753786	39.0	37.0	39.0	34.0	39.0
11	37.162981721121255	39.0	37.0	39.0	34.0	39.0
12	37.15051356911822	39.0	37.0	39.0	34.0	39.0
13	37.03302124232357	39.0	37.0	39.0	33.0	39.0
14	38.14129797850728	40.0	37.0	41.0	33.0	41.0
15	38.039810318880086	40.0	37.0	41.0	33.0	41.0
16	38.26143881957835	40.0	37.0	41.0	34.0	41.0
17	38.26294377457168	40.0	37.0	41.0	34.0	41.0
18	38.354691819808096	40.0	37.0	41.0	34.0	41.0
19	38.364524364524364	40.0	37.0	41.0	34.0	41.0
20	38.348416441439696	40.0	37.0	41.0	34.0	41.0
21	38.210755652616115	40.0	37.0	41.0	34.0	41.0
22	38.28861061419201	40.0	37.0	41.0	34.0	41.0
23	38.25174825174825	40.0	37.0	41.0	34.0	41.0
24	38.22962825288407	40.0	37.0	41.0	34.0	41.0
25	37.98484461275159	40.0	37.0	41.0	34.0	41.0
26	37.818068236672886	40.0	36.0	41.0	33.0	41.0
27	36.98996610624518	40.0	36.0	41.0	32.0	41.0
28	37.319930715279554	39.0	36.0	41.0	32.0	41.0
29	37.65126346521696	40.0	36.0	41.0	33.0	41.0
30	37.698936722192535	39.0	36.0	41.0	33.0	41.0
31	37.671246066594904	39.0	36.0	41.0	33.0	41.0
32	37.66287975590301	39.0	36.0	41.0	33.0	41.0
33	36.43612976171116	39.0	35.0	41.0	31.0	41.0
34	36.959392029159474	39.0	35.0	41.0	31.0	41.0
35	37.307651005325425	39.0	35.0	41.0	32.0	41.0
36	37.355195192404494	39.0	35.0	41.0	33.0	41.0
37	37.441591999731536	39.0	35.0	41.0	33.0	41.0
38	37.415179136109366	39.0	35.0	40.0	33.0	41.0
39	37.47203184412487	39.0	36.0	41.0	33.0	41.0
40	37.505024691071206	39.0	36.0	41.0	33.0	41.0
41	37.42661731033824	39.0	36.0	41.0	33.0	41.0
42	37.39272097411632	39.0	35.0	41.0	33.0	41.0
43	37.25798232774977	39.0	35.0	40.0	33.0	41.0
44	37.3088952391278	39.0	35.0	40.0	33.0	41.0
45	37.34023341000085	39.0	35.0	40.0	33.0	41.0
46	37.31728736379899	39.0	35.0	40.0	33.0	41.0
47	37.246435734807825	39.0	35.0	40.0	33.0	41.0
48	37.201044433602576	39.0	35.0	40.0	33.0	41.0
49	37.24402729053892	39.0	35.0	40.0	33.0	41.0
50	37.188852491178075	39.0	35.0	40.0	33.0	41.0
51	36.48381850707432	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	6.0
12	6.0
13	7.0
14	9.0
15	9.0
16	11.0
17	17.0
18	34.0
19	58.0
20	141.0
21	214.0
22	332.0
23	505.0
24	829.0
25	1170.0
26	1688.0
27	2165.0
28	2802.0
29	4041.0
30	5666.0
31	8166.0
32	12116.0
33	20269.0
34	27335.0
35	37184.0
36	46637.0
37	70449.0
38	80929.0
39	64548.0
40	38.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.520149101544447	4.277118230606602	54.6876895714105	17.515043096438447
2	19.727042982856936	3.961155123945822	53.36007661589057	22.95172527730667
3	21.00431867873728	4.428388149318382	52.4163175325966	22.150975639347735
4	20.110122435703833	4.669490716002344	49.98257556397091	25.237811284322913
5	14.19794675608629	21.847919522338128	42.68986827126362	21.26426545031196
6	15.612810961648172	29.25859670045717	40.5731219684708	14.555470369423858
7	78.9471510401743	2.019943880408997	12.445177561456632	6.587727517960077
8	78.45591101405056	2.425481495248937	10.444594165524398	8.674013325176116
9	72.70817038258899	4.984937543077078	14.240539821935172	8.066352252398765
10	34.55691595226479	27.176699269722526	21.69019610880076	16.576188669211923
11	26.65112665112665	25.93762826320966	27.12197363360154	20.28927145206215
12	25.21716010088103	20.725785842064912	32.996977183023695	21.06007687403036
13	21.994026645189436	25.549386014502296	32.41719520789289	20.039392132415387
14	17.52924078505474	28.847638149963732	30.502314223244454	23.120806841737075
15	15.606873746408631	26.010681824635313	36.15273615273615	22.229708276219903
16	16.7845074821819	25.70117221280012	32.723090862625746	24.791229442392233
17	16.585223561967748	25.32970905063928	29.637287776822664	28.447779610570308
18	18.1304483630065	23.629084094200373	33.82741289718034	24.413054645612785
19	19.374424025586816	25.56280928373952	31.15334278124976	23.90942390942391
20	22.05598019551508	25.9995818135353	30.81853546969826	21.12590252125136
21	21.106025757188547	27.325129650711045	30.215004633609283	21.353839958491122
22	18.717458252341974	25.674325674325676	28.672877510086813	26.93533856324554
23	18.29256015302527	25.751509472439704	28.4147377170633	27.541192657471726
24	21.581261116144837	23.748086538784214	29.02317320921972	25.64747913585123
25	17.802094546280593	26.269337897244878	28.111939739846715	27.816627816627815
26	18.134062320108832	29.631092421790093	27.815853397248745	24.418991860852326
27	20.391752949892485	29.908076419704326	27.167148097380654	22.533022533022535
28	16.846977312093593	27.875483689437182	31.123656705052056	24.15388229341718
29	18.29565783054155	25.619600038204688	32.050120422213446	24.034621709040312
30	25.298990415269486	25.4572301083929	27.742025416444022	21.501754059893592
31	25.137911184422812	28.71727755448686	24.238552145528892	21.90625911556144
32	25.307509028439263	27.589722938560147	25.513504583272024	21.589263449728566
33	25.112871624499533	26.319933296677483	26.902812949324577	21.664382129498406
34	22.15020121996866	24.90919932780398	29.053117425210452	23.887482027016908
35	23.13009987428592	23.709365569830688	28.06056992103504	25.099964634848355
36	26.752575589784893	25.584493026353496	27.48336934383446	20.179562040027157
37	23.55241657567239	27.100031751194543	30.247530247530246	19.10002142560282
38	22.42641079850382	27.188057420615557	28.99348713302202	21.3920446478586
39	24.01448680518448	22.6863575700785	31.643808387994433	21.655347236742585
40	28.102130427711824	21.531181996298276	28.28282828282828	22.08385929316162
41	24.031007751937985	23.83895174592849	26.04423999772837	26.085800504405153
42	24.541350122745474	22.804843735076293	28.166407236174678	24.48739890600356
43	23.966730943475127	22.574324899906294	30.114588254123138	23.344355902495437
44	21.301437580507347	23.92852625410765	29.59443657118076	25.175599594204247
45	23.284725610307007	22.31541068750371	27.35068549022037	27.04917821196891
46	26.297991414270484	22.631890073750537	29.58669237739005	21.483426134588925
47	21.611721611721613	23.581586372284047	32.501348780418546	22.305343235575794
48	21.828042758275316	21.353839958491122	32.15544145776704	24.662675825466522
49	23.330932633258215	19.301628603954185	34.43584838933676	22.931590373450838
50	24.159819508656717	19.587389354831217	30.899849504500665	25.3529416320114
51	22.712429689173874	19.101828404153988	27.481304225490273	30.70443768118187
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	75.0
1	164.0
2	253.0
3	548.5
4	844.0
5	624.0
6	404.0
7	462.0
8	520.0
9	610.5
10	701.0
11	716.0
12	731.0
13	759.0
14	787.0
15	799.5
16	812.0
17	821.0
18	830.0
19	783.5
20	737.0
21	918.5
22	1100.0
23	1249.5
24	1399.0
25	1544.5
26	2022.0
27	2354.0
28	2789.0
29	3224.0
30	3910.0
31	4596.0
32	5449.5
33	6303.0
34	7372.5
35	8442.0
36	9019.0
37	9596.0
38	11404.5
39	13213.0
40	16050.5
41	18888.0
42	22557.0
43	26226.0
44	30676.0
45	35126.0
46	41363.5
47	47601.0
48	53994.0
49	60387.0
50	55007.5
51	49628.0
52	39233.5
53	28839.0
54	23370.0
55	17901.0
56	14850.0
57	11799.0
58	10629.0
59	9459.0
60	8568.0
61	7677.0
62	6430.0
63	5183.0
64	4407.0
65	3631.0
66	3085.5
67	2540.0
68	2033.0
69	1526.0
70	1313.5
71	1101.0
72	842.0
73	583.0
74	466.0
75	241.0
76	133.0
77	103.0
78	73.0
79	54.0
80	35.0
81	52.0
82	69.0
83	42.5
84	16.0
85	10.0
86	4.0
87	3.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	387387.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.24619455021142
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.04847545986695	30.437739113236717
2	12.012659123577201	10.149782688000291
3	4.576111952892532	5.799699475363374
4	2.5437224924816637	4.298503811965163
5	1.6335810295473103	3.450629099389517
6	1.1795246469488367	2.9898256627062
7	0.9089659488940012	2.688024662154545
8	0.7307861880453524	2.469834837981708
9	0.6170595678090931	2.346157669565909
>10	3.6108304465485985	25.672452600170757
>50	0.08697446575116088	2.5836691456698526
>100	0.044425806246995846	3.354223223798318
>500	0.0037542934856616213	1.130057867581197
>1k	0.0031285779047180175	2.6294001424164133
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	3099	0.7999752185798699	No Hit
CTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	2325	0.6001750187796699	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	1877	0.4845283915051357	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	1512	0.3903073670515531	No Hit
CCTGTATCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	1134	0.29273052528866483	No Hit
CTGTCACTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	871	0.22483975972348064	Illumina Single End Adapter 1 (95% over 21bp)
TCTGTATCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	841	0.2170955659327752	No Hit
TCCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	768	0.19825136104205873	No Hit
GCTGTATCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	670	0.17295366132575435	No Hit
CGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	602	0.1554001554001554	No Hit
CCTGACTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGC	523	0.13500711175129781	No Hit
CTGTATCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTGCT	427	0.11022569162104047	Illumina Single End Adapter 1 (95% over 21bp)
TGCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCTG	419	0.10816057327685234	No Hit
TTCCTGTCTCTTATACACATCTGACGCAGATGGGTTCGTATGCCGTCTTCT	400	0.10325591720940558	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6458657621448319	0.0
2	0.0	0.0	0.0	2.4895001639187684	0.0
3	0.0	0.0	0.0	3.4381639032801825	0.0
4	0.0	0.0	0.0	4.382181126367173	0.0
5	0.0	0.0	0.0	5.881198904454719	0.0
6	0.0	0.0	0.0	6.957383701569748	0.0
7	0.0	0.0	0.0	8.333526938178101	0.0
8	0.0	0.0	0.0	10.482798854891879	0.0
9	0.0	0.0	0.0	11.428623056530034	0.0
10	0.0	0.0	0.0	13.24876673713883	0.0
11	2.5813979302351394E-4	0.0	0.0	16.418207115881536	0.0
12	2.5813979302351394E-4	0.0	0.0	19.181077320612204	0.0
13	2.5813979302351394E-4	0.0	0.0	20.130773619145714	0.0
14	2.5813979302351394E-4	0.0	0.0	20.427634381122754	0.0
15	2.5813979302351394E-4	0.0	0.0	20.956046537441885	0.0
16	2.5813979302351394E-4	0.0	0.0	22.177047758443106	0.0
17	2.5813979302351394E-4	0.0	0.0	23.896516919772733	0.0
18	2.5813979302351394E-4	0.0	0.0	25.96292596292596	0.0
19	2.5813979302351394E-4	0.0	0.0	27.135138763045738	0.0
20	2.5813979302351394E-4	0.0	0.0	28.416802835407488	0.0
21	2.5813979302351394E-4	0.0	0.0	29.93027644190435	0.0
22	2.5813979302351394E-4	0.0	0.0	31.385410455177897	0.0
23	2.5813979302351394E-4	0.0	0.0	32.79020720881186	0.0
24	2.5813979302351394E-4	0.0	0.0	33.85838967234316	0.0
25	2.5813979302351394E-4	0.0	0.0	34.78382083033246	0.0
26	2.5813979302351394E-4	0.0	0.0	35.59696117835653	0.0
27	2.5813979302351394E-4	0.0	0.0	36.354859610673564	0.0
28	2.5813979302351394E-4	0.0	0.0	37.171097636213915	0.0
29	2.5813979302351394E-4	0.0	0.0	38.01469847981476	0.0
30	2.5813979302351394E-4	0.0	0.0	38.92825520732497	0.0
31	2.5813979302351394E-4	0.0	0.0	39.79612119147003	0.0
32	2.5813979302351394E-4	0.0	0.0	40.59635454984292	0.0
33	2.5813979302351394E-4	0.0	0.0	41.36148089636462	0.0
34	2.5813979302351394E-4	0.0	0.0	42.151646802809594	0.0
35	2.5813979302351394E-4	0.0	0.0	42.97201506503832	0.0
36	2.5813979302351394E-4	0.0	0.0	43.74204606762746	0.0
37	2.5813979302351394E-4	0.0	0.0	44.45812585347469	0.0
38	2.5813979302351394E-4	0.0	0.0	45.14477770291724	0.0
39	2.5813979302351394E-4	0.0	0.0	45.88770402723891	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTTACG	30	2.1617616E-6	45.000004	1
GCGTCAT	35	1.2091368E-7	45.000004	37
TGCGTAG	30	2.1617616E-6	45.000004	1
CAGGTTT	20	7.02749E-4	45.0	5
CTTAAGG	25	3.886001E-5	45.0	2
AACCGAG	25	3.886001E-5	45.0	16
CTGTCGT	20	7.02749E-4	45.0	23
TCGTTAG	75	0.0	45.0	1
CGAACGA	25	3.886001E-5	45.0	22
CTGTACG	20	7.02749E-4	45.0	1
GATCGAA	20	7.02749E-4	45.0	13
CGAAAGG	20	7.02749E-4	45.0	16
ATTACGT	20	7.02749E-4	45.0	34
TCTTACG	45	3.8380676E-10	45.0	1
CACGCCA	25	3.886001E-5	45.0	27
CCTACGG	20	7.02749E-4	45.0	2
CGCGAGG	25	3.886001E-5	45.0	2
TAACGAA	25	3.886001E-5	45.0	19
GCGTTAG	20	7.02749E-4	45.0	1
AGAGTAA	45	3.8380676E-10	45.0	41
>>END_MODULE