FastQCFastQC Report
Sat 14 Jan 2017
SRR2934125.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934125.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1697634
Sequences flagged as poor quality0
Sequence length51
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGC265691.56506054897581No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCG207581.2227606186021251No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC179661.05829642903005RNA PCR Primer, Index 46 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCGAATAT80570.47460171038044713No Hit
GAATCAGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC67900.39996842664555493RNA PCR Primer, Index 46 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTC56350.33193256025739354No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCT52590.30978408773622584RNA PCR Primer, Index 46 (95% over 21bp)
GAACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCT43740.2576527095946476TruSeq Adapter, Index 8 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC42290.2491114103511122RNA PCR Primer, Index 40 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCGAATATCGTA42130.24816892215872208No Hit
GAATATGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC42100.24799220562264895RNA PCR Primer, Index 46 (95% over 23bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGC32450.19114838651912014No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24050.14166775641863913No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCG23160.1364251658484691No Hit
CTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGCT22900.13489362253583517RNA PCR Primer, Index 46 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC22450.13224287449473796RNA PCR Primer, Index 46 (96% over 26bp)
GCTGTATCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC18000.1060299216438879TruSeq Adapter, Index 8 (95% over 23bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAATCG207.0342375E-445.00000416
TACTACG3300.043.6363644
CGATCGA1000.042.7541
AATACGG12150.042.592595
CCGATGA33450.041.9058318
TTACGGG13400.041.641793
CGATGAA34100.041.4369519
TACGGCT37500.041.47
TACGGGT2350.041.170214
GATGAAT37000.041.047320
CGTTATT4850.040.8247381
ATACTAC4850.040.8247383
TTTACGG9000.040.752
TAATACG12700.040.748034
GTACGGG6300.040.7142833
TAGGGAT14050.040.676165
ACTACGG3550.040.5633775
TACGGGA7050.040.5319184
TGATACC24950.040.4909824
TTAGGGA26000.040.2403874
Produced by FastQC (version 0.11.5)