##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2934125.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1697634 Sequences flagged as poor quality 0 Sequence length 51 %GC 49 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.763957366546617 31.0 31.0 34.0 30.0 34.0 2 31.856514419480288 33.0 31.0 34.0 30.0 34.0 3 31.964475263808335 33.0 31.0 34.0 30.0 34.0 4 35.58746467141916 37.0 35.0 37.0 33.0 37.0 5 29.443599150346895 35.0 35.0 37.0 0.0 37.0 6 23.731866232650855 35.0 17.0 37.0 0.0 37.0 7 30.325524229604262 35.0 19.0 37.0 17.0 37.0 8 33.70968654020832 35.0 32.0 37.0 28.0 37.0 9 36.61176083890874 37.0 35.0 39.0 33.0 39.0 10 36.832516313881555 38.0 37.0 39.0 33.0 39.0 11 36.98050580985065 39.0 37.0 39.0 33.0 39.0 12 36.886336513052875 39.0 35.0 39.0 33.0 39.0 13 36.72893862870324 39.0 35.0 39.0 33.0 39.0 14 37.62026326051434 40.0 37.0 41.0 32.0 41.0 15 37.502114707881674 40.0 36.0 41.0 32.0 41.0 16 37.90339554933513 40.0 37.0 41.0 33.0 41.0 17 37.83663027484134 40.0 36.0 41.0 33.0 41.0 18 37.94668285390137 40.0 36.0 41.0 33.0 41.0 19 37.93929139025255 40.0 37.0 41.0 33.0 41.0 20 37.661271510820356 40.0 36.0 41.0 32.0 41.0 21 37.707606586578734 40.0 36.0 41.0 33.0 41.0 22 37.83971162217534 40.0 36.0 41.0 33.0 41.0 23 37.67249183275076 39.0 36.0 41.0 33.0 41.0 24 37.66640807146888 40.0 36.0 41.0 33.0 41.0 25 37.52072884968138 39.0 35.0 41.0 33.0 41.0 26 37.396606100019206 39.0 35.0 41.0 33.0 41.0 27 36.458145866541315 39.0 35.0 41.0 31.0 41.0 28 36.810886798921324 39.0 35.0 41.0 31.0 41.0 29 37.093354633566484 39.0 35.0 41.0 31.0 41.0 30 37.06234029243053 39.0 35.0 41.0 31.0 41.0 31 37.14560323367699 39.0 35.0 41.0 32.0 41.0 32 36.96229575986343 39.0 35.0 41.0 31.0 41.0 33 35.6927771239266 39.0 35.0 41.0 29.0 41.0 34 36.067497470008256 39.0 35.0 41.0 29.0 41.0 35 36.34055102572168 39.0 35.0 41.0 30.0 41.0 36 36.463746602624596 39.0 35.0 40.0 30.0 41.0 37 36.591366572535655 39.0 35.0 40.0 31.0 41.0 38 36.56622511094853 39.0 35.0 40.0 31.0 41.0 39 36.40512854949889 39.0 35.0 40.0 31.0 41.0 40 36.265335755527985 39.0 35.0 40.0 30.0 41.0 41 36.06033338163585 39.0 35.0 40.0 29.0 41.0 42 36.212906315495566 39.0 35.0 40.0 30.0 41.0 43 36.15327214228744 39.0 35.0 40.0 30.0 41.0 44 36.32112811124188 39.0 35.0 40.0 31.0 41.0 45 36.24279615040698 39.0 35.0 40.0 31.0 41.0 46 36.14534640564456 38.0 35.0 40.0 31.0 41.0 47 35.97784092448667 38.0 35.0 40.0 30.0 41.0 48 35.9560270352738 38.0 35.0 40.0 30.0 41.0 49 35.9886489078329 38.0 35.0 40.0 30.0 41.0 50 35.899500716880084 38.0 35.0 40.0 30.0 41.0 51 35.19167264557614 37.0 34.0 40.0 28.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 2.0 9 15.0 10 8.0 11 13.0 12 30.0 13 30.0 14 45.0 15 63.0 16 101.0 17 175.0 18 332.0 19 770.0 20 1681.0 21 3798.0 22 6812.0 23 9903.0 24 12717.0 25 14171.0 26 15483.0 27 16659.0 28 18944.0 29 23789.0 30 32338.0 31 45507.0 32 64408.0 33 99685.0 34 132017.0 35 153899.0 36 199813.0 37 283662.0 38 309568.0 39 251052.0 40 144.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 31.91912980065197 3.763237541189679 48.20367641081646 16.11395624734189 2 18.75469035139494 14.951809400612854 46.32400152211843 19.969498725873773 3 19.198307762450565 14.939203621039635 45.7100293702883 20.152459246221504 4 17.7766232297421 4.101472991233682 54.96349625419848 23.158407524825726 5 21.366384038019973 21.857066953183075 36.31677970634424 20.459769302452706 6 15.64913285195749 35.29959932470721 35.977896295667975 13.07337152766733 7 76.0690466849745 2.202594905615698 17.28794310198783 4.4404153074219765 8 76.01408784225575 9.953676705344026 8.795771055480746 5.2364643969194775 9 71.586278314407 4.304461385669703 9.759052893615468 14.350207406307838 10 45.42899117241997 21.34211496706593 17.659577977349652 15.56931588316445 11 35.27002875767097 20.400039113259986 24.265183190251843 20.0647489388172 12 27.4036099653989 17.671889229362748 29.028754136639584 25.89574666859877 13 28.358880653898304 18.076039947362034 34.721147196627776 18.84393220211188 14 22.08308740282063 24.60695297101731 31.227755806021797 22.08220382014027 15 18.388415877627335 20.346847435901967 37.394809481902456 23.86992720456824 16 22.93008976021922 21.02249365882163 28.118722881374904 27.928693699584244 17 23.55054151837204 23.715712574088407 29.4035110041387 23.33023490340085 18 23.480031620478854 20.73432789399835 29.672414666530006 26.113225818992785 19 23.34714078535185 23.136965918448855 27.58115117864039 25.934742117558905 20 24.98736476767077 26.275863937692108 29.971242329029696 18.765528965607427 21 23.86698192896702 27.825550148029553 29.167417711944978 19.140050211058448 22 21.914794354966972 19.866119552270984 33.90442227240972 24.314663820352326 23 23.462183250335467 24.10649174085816 30.039101478881786 22.39222352992459 24 22.988641839171457 25.192355949515623 26.606677293221036 25.212324918091884 25 22.623898908716484 29.142265058310567 24.08257610297626 24.15125992999669 26 20.61410174395659 22.238362332516903 28.8674119392048 28.28012398432171 27 23.41046421077806 24.692778302036835 27.294045713033547 24.602711774151555 28 18.372864822452897 24.3576059386181 29.649794949912646 27.619734289016364 29 22.628316822118315 21.653784031186934 27.602946218089414 28.11495292860534 30 22.83330800396316 25.357585910744014 26.90721321556943 24.90189286972339 31 24.170050788332468 21.639411086252984 24.928282539110313 29.26225558630423 32 28.702829938608676 24.428940513679628 23.439386817182033 23.428842730529666 33 24.49414891549062 26.334828355228513 22.665014956109502 26.50600777317137 34 22.626019507149362 24.334043733808347 26.310029134666244 26.729907624376043 35 22.407656774074976 24.153851772525762 26.231920425721917 27.206571027677345 36 21.9525527881746 27.093531350102555 22.922373138144028 28.031542723578816 37 21.915913559695436 24.65761171135828 26.34955473323461 27.07691999571168 38 22.864940263920257 24.784258562210702 23.74357488127594 28.6072262925931 39 24.699729152455713 26.18897830745614 24.33316015112798 24.778132388960163 40 21.63422740119484 24.558944978717438 28.769746600268377 25.037081019819347 41 20.065750332521613 27.61690682443919 25.317706879103508 26.99963596393569 42 20.57557753909264 21.64777566896045 32.588001889688826 25.188644902258083 43 21.381346038074167 24.596467789876968 27.045169924730537 26.977016247318325 44 22.252794182962877 20.88524381580482 29.0099632783038 27.8519987229285 45 20.97366098935342 19.785301189773534 26.682076348612245 32.558961472260805 46 25.492950777376045 22.97456342179763 26.28428742591159 25.248198374914733 47 18.002408057331557 24.147489977227128 33.43812623922471 24.411975726216607 48 20.13525883671039 26.27232960697064 26.94885941257067 26.643552143748302 49 22.18658438744747 20.90085377649128 32.38713409368568 24.525427742375566 50 22.200368277261177 22.855279759948257 27.335691910034786 27.60866005275578 51 20.36634516038204 22.049216733406613 26.42206741853662 31.162370687674727 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 248.0 1 936.5 2 1625.0 3 4593.5 4 7562.0 5 5283.5 6 3005.0 7 2900.0 8 2795.0 9 2794.0 10 2793.0 11 2790.0 12 2787.0 13 2780.5 14 2774.0 15 2696.0 16 2618.0 17 2667.5 18 2717.0 19 2912.0 20 3107.0 21 3496.0 22 3885.0 23 4403.0 24 4921.0 25 6173.0 26 9017.5 27 10610.0 28 12248.0 29 13886.0 30 16281.0 31 18676.0 32 22106.5 33 25537.0 34 29112.0 35 32687.0 36 35763.5 37 38840.0 38 41730.0 39 44620.0 40 49820.0 41 55020.0 42 62889.0 43 70758.0 44 88030.0 45 105302.0 46 163880.0 47 222458.0 48 196614.0 49 170770.0 50 164224.0 51 157678.0 52 143547.5 53 129417.0 54 122322.5 55 115228.0 56 107849.0 57 100470.0 58 93536.5 59 86603.0 60 77455.0 61 68307.0 62 62397.5 63 56488.0 64 49215.5 65 41943.0 66 36289.5 67 30636.0 68 25356.0 69 20076.0 70 16886.5 71 13697.0 72 11145.5 73 8594.0 74 6863.5 75 4228.5 76 3324.0 77 2502.5 78 1681.0 79 1095.5 80 510.0 81 364.0 82 218.0 83 160.0 84 102.0 85 93.5 86 85.0 87 46.5 88 8.0 89 8.5 90 9.0 91 5.0 92 1.0 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 1697634.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.160951842645616 #Duplication Level Percentage of deduplicated Percentage of total 1 72.23635677926067 24.67662705224491 2 10.029298365923479 6.852207569876727 3 3.858330844608611 3.954127625270069 4 2.2096461930919893 3.019344687660026 5 1.5405044758657565 2.631254960671507 6 1.2159596042607093 2.4923002490251505 7 1.065583756596703 2.548094877538376 8 0.912308531652455 2.4932262252331396 9 0.7758469061512622 2.385330191846897 >10 6.0312541119551515 34.88807723680302 >50 0.0758387579771504 1.7712819184160975 >100 0.04063135462006269 2.499012472863476 >500 0.0035331563871748213 0.8651220005463897 >1k 0.0035331563871748213 2.819860534101524 >5k 7.851458638166271E-4 1.7260196416109608 >10k+ 5.888593978624702E-4 4.378112756291692 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGC 26569 1.56506054897581 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCG 20758 1.2227606186021251 No Hit GAATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC 17966 1.05829642903005 RNA PCR Primer, Index 46 (95% over 23bp) GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCCGAATAT 8057 0.47460171038044713 No Hit GAATCAGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC 6790 0.39996842664555493 RNA PCR Primer, Index 46 (95% over 23bp) GAATGATCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTC 5635 0.33193256025739354 No Hit GAATGACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCT 5259 0.30978408773622584 RNA PCR Primer, Index 46 (95% over 21bp) GAACTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCT 4374 0.2576527095946476 TruSeq Adapter, Index 8 (95% over 21bp) GCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC 4229 0.2491114103511122 RNA PCR Primer, Index 40 (95% over 23bp) GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCGAATATCGTA 4213 0.24816892215872208 No Hit GAATATGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTC 4210 0.24799220562264895 RNA PCR Primer, Index 46 (95% over 23bp) GAATAATACGGCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGC 3245 0.19114838651912014 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2405 0.14166775641863913 No Hit GAATAATACCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCG 2316 0.1364251658484691 No Hit CTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGCT 2290 0.13489362253583517 RNA PCR Primer, Index 46 (96% over 27bp) CCTGTCTCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC 2245 0.13224287449473796 RNA PCR Primer, Index 46 (96% over 26bp) GCTGTATCTTATACACATCTGACGCCCCGAATATCGTATGCCGTCTTCTGC 1800 0.1060299216438879 TruSeq Adapter, Index 8 (95% over 23bp) >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.890551202438217E-5 0.0 0.0 0.14420069343568756 0.0 2 5.890551202438217E-5 0.0 0.0 0.6559128763914954 0.0 3 5.890551202438217E-5 0.0 0.0 0.8659110267584179 0.0 4 5.890551202438217E-5 0.0 0.0 1.3663133514055443 0.0 5 5.890551202438217E-5 0.0 0.0 2.9277217586358426 0.0 6 5.890551202438217E-5 0.0 0.0 3.515834390687274 0.0 7 5.890551202438217E-5 0.0 0.0 4.497141315501457 0.0 8 5.890551202438217E-5 0.0 0.0 5.787348745371499 0.0 9 5.890551202438217E-5 0.0 0.0 6.268253345538556 0.0 10 1.1781102404876434E-4 0.0 0.0 8.569809511355215 0.0 11 1.1781102404876434E-4 0.0 0.0 10.010756146495652 0.0 12 1.1781102404876434E-4 0.0 0.0 13.31977328446532 0.0 13 1.1781102404876434E-4 0.0 0.0 13.868360317948392 0.0 14 1.1781102404876434E-4 0.0 0.0 14.139914728380793 0.0 15 1.1781102404876434E-4 0.0 0.0 14.696984155595375 0.0 16 1.1781102404876434E-4 0.0 0.0 15.278204842739955 0.0 17 1.1781102404876434E-4 0.0 0.0 15.929523089193548 0.0 18 1.1781102404876434E-4 0.0 0.0 16.56776431197773 0.0 19 1.1781102404876434E-4 0.0 0.0 17.703050245223647 0.0 20 1.1781102404876434E-4 0.0 0.0 18.301765869439468 0.0 21 1.1781102404876434E-4 0.0 0.0 19.046213730403608 0.0 22 1.1781102404876434E-4 0.0 0.0 19.90193410358181 0.0 23 1.1781102404876434E-4 0.0 0.0 20.634306334580952 0.0 24 1.1781102404876434E-4 0.0 0.0 21.25369779351733 0.0 25 1.1781102404876434E-4 0.0 0.0 21.782669291496283 0.0 26 1.1781102404876434E-4 0.0 0.0 22.261158765670338 0.0 27 1.1781102404876434E-4 0.0 0.0 22.826121531496188 0.0 28 1.1781102404876434E-4 0.0 0.0 23.31650991909917 0.0 29 1.1781102404876434E-4 0.0 0.0 23.890426322752724 0.0 30 1.1781102404876434E-4 0.0 0.0 24.557236718868733 0.0 31 1.1781102404876434E-4 0.0 0.0 25.07436820893078 0.0 32 1.1781102404876434E-4 0.0 0.0 25.592442187185224 0.0 33 1.1781102404876434E-4 0.0 0.0 26.120176669411663 0.0 34 1.1781102404876434E-4 0.0 0.0 26.70687556917451 0.0 35 1.1781102404876434E-4 0.0 0.0 27.347826445511814 0.0 36 1.1781102404876434E-4 0.0 0.0 27.912023439681345 0.0 37 1.1781102404876434E-4 0.0 0.0 28.474335457466097 0.0 38 1.1781102404876434E-4 0.0 0.0 29.17442746787588 0.0 39 1.1781102404876434E-4 0.0 0.0 30.49391093722204 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGAATCG 20 7.0342375E-4 45.000004 16 TACTACG 330 0.0 43.636364 4 CGATCGA 100 0.0 42.75 41 AATACGG 1215 0.0 42.59259 5 CCGATGA 3345 0.0 41.90583 18 TTACGGG 1340 0.0 41.64179 3 CGATGAA 3410 0.0 41.43695 19 TACGGCT 3750 0.0 41.4 7 TACGGGT 235 0.0 41.17021 4 GATGAAT 3700 0.0 41.0473 20 CGTTATT 485 0.0 40.824738 1 ATACTAC 485 0.0 40.824738 3 TTTACGG 900 0.0 40.75 2 TAATACG 1270 0.0 40.74803 4 GTACGGG 630 0.0 40.714283 3 TAGGGAT 1405 0.0 40.67616 5 ACTACGG 355 0.0 40.563377 5 TACGGGA 705 0.0 40.531918 4 TGATACC 2495 0.0 40.490982 4 TTAGGGA 2600 0.0 40.240387 4 >>END_MODULE