SRR2934124.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934124.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1732828
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	39930	2.3043256457074794	RNA PCR Primer, Index 21 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC	36866	2.127504864879838	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCG	32001	1.8467499370970462	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15386	0.8879127068583841	No Hit
GAATCAGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	14935	0.8618858882705034	RNA PCR Primer, Index 21 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTTCGATCT	11599	0.6693682235051603	No Hit
GAACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	11029	0.6364740181945352	RNA PCR Primer, Index 14 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTC	10391	0.5996555918994845	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTT	10101	0.5829199435835524	RNA PCR Primer, Index 21 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCT	9983	0.5761102659929318	RNA PCR Primer, Index 21 (95% over 21bp)
CGTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	8027	0.46323120355857594	RNA PCR Primer, Index 14 (95% over 22bp)
GAATATGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	7921	0.4571140355534421	RNA PCR Primer, Index 21 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCT	7890	0.45532505245760113	RNA PCR Primer, Index 21 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	7407	0.42745154164175553	TruSeq Adapter, Index 21 (96% over 25bp)
CGTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7229	0.4171793161237006	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTA	6336	0.3656450611370546	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	5555	0.3205742289482857	RNA PCR Primer, Index 21 (96% over 25bp)
GAATGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTT	4702	0.27134833924659574	RNA PCR Primer, Index 21 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT	4655	0.26863601003677223	RNA PCR Primer, Index 21 (96% over 27bp)
CCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	4552	0.2626919694280102	RNA PCR Primer, Index 21 (96% over 26bp)
GAACTATCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	4281	0.2470527946224322	RNA PCR Primer, Index 21 (95% over 24bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGC	4123	0.2379347517468554	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCT	3787	0.21854448335322374	RNA PCR Primer, Index 21 (95% over 24bp)
CGTTTATGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTT	3701	0.21358149799056803	RNA PCR Primer, Index 21 (95% over 22bp)
GAATAATACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCG	3386	0.1954031213715383	No Hit
GCTGTATCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	3046	0.17578201644941102	RNA PCR Primer, Index 14 (96% over 25bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTC	3044	0.1756665981851632	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCC	3035	0.17514721599604807	No Hit
CGTTTACTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCT	3005	0.17341594203233096	RNA PCR Primer, Index 21 (95% over 21bp)
CGTTCAGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTC	2808	0.1620472430039219	RNA PCR Primer, Index 14 (95% over 22bp)
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTTCGATCTCGT	2712	0.15650716632002715	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGT	2650	0.1529292001283451	No Hit
TCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGC	2445	0.14109882804294482	TruSeq Adapter, Index 21 (96% over 25bp)
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCTTCGATCTCGTATG	2049	0.11824601172187892	No Hit
CGCTGACTCTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTG	1810	0.10445352914426592	RNA PCR Primer, Index 14 (95% over 24bp)
CTGTCACTTATACACATCTGACGCCTTCGATCTCGTATGCCGTCTTCTGCT	1733	0.1000099259707253	RNA PCR Primer, Index 21 (96% over 27bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTCGCTA	20	7.034278E-4	45.000004	1
TCGAATA	45	3.8562575E-10	45.000004	44
GTCTACG	50	2.1827873E-11	45.0	1
CATACCG	25	3.8916292E-5	45.0	12
TCGCGAG	25	3.8916292E-5	45.0	1
CGACGGA	30	2.1661435E-6	44.999996	41
CGTTATT	2990	0.0	44.698997	1
CGTTTAT	4130	0.0	44.673126	1
CGTTTTT	7790	0.0	43.555843	1
ATCAGTC	1830	0.0	43.15574	3
CCGCTCG	120	0.0	43.124996	40
CGCTCGA	120	0.0	43.124996	41
CGTTATC	210	0.0	42.857143	1
CCGATGA	2925	0.0	42.76923	18
TACGACG	100	0.0	42.75	29
CGTTTTC	900	0.0	42.75	1
AATACGG	1390	0.0	42.733814	5
GTTATTT	3105	0.0	42.391304	2
ACCCGTC	85	0.0	42.352943	39
TGCGTAG	80	0.0	42.187504	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content