SRR2934123.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934123.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	883546
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC	12894	1.4593467685892982	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG	10595	1.199145262385886	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	9388	1.0625366421216327	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	4720	0.5342110088212725	TruSeq Adapter, Index 19 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	4456	0.5043314100227945	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTCCTTT	4371	0.4947110846520724	No Hit
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	4241	0.4799976458497916	TruSeq Adapter, Index 16 (95% over 23bp)
GAATCAGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	3564	0.4033745837794523	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTC	3090	0.34972712230036695	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT	2878	0.32573289902280134	No Hit
TCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	2762	0.31260398439922765	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTA	2402	0.2718590769467577	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	2355	0.2665396029182408	No Hit
GCTGTATCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1945	0.22013568054181673	TruSeq Adapter, Index 16 (95% over 22bp)
GAATATGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	1938	0.21934341845246313	No Hit
CCTGTATCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1635	0.18504978801330094	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCACTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	1466	0.1659223175703359	TruSeq Adapter, Index 16 (95% over 23bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC	1287	0.14566304414258002	No Hit
TCTGTATCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1137	0.12868599937071754	TruSeq Adapter, Index 16 (95% over 22bp)
GAATAATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG	1090	0.12336652534220063	No Hit
TCCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	1021	0.1155570847471439	TruSeq Adapter, Index 16 (95% over 21bp)
GAACTATCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	989	0.11193531519581323	No Hit
GCTGACTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	956	0.1082003653460035	TruSeq Adapter, Index 19 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	891	0.10084364594486307	TruSeq Adapter, Index 16 (95% over 21bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGACA	30	2.1649303E-6	45.000004	30
CATACGC	30	2.1649303E-6	45.000004	32
CGTCAGG	30	2.1649303E-6	45.000004	2
AACGTAC	20	7.0324005E-4	45.0	37
AAGTACG	20	7.0324005E-4	45.0	10
CGCGCAT	25	3.8900707E-5	45.0	36
CGCGAAC	20	7.0324005E-4	45.0	11
CCCGTAA	20	7.0324005E-4	45.0	36
GGACGAT	20	7.0324005E-4	45.0	8
CGATCGA	25	3.8900707E-5	45.0	41
GGTTACG	20	7.0324005E-4	45.0	1
TCGAATA	20	7.0324005E-4	45.0	44
CCGTTAG	25	3.8900707E-5	45.0	1
CGAAGTT	20	7.0324005E-4	45.0	23
CGTTCGG	65	0.0	44.999996	2
AATACGG	345	0.0	44.347824	5
TCGTTTG	200	0.0	42.75	1
AGGGATT	550	0.0	42.545452	6
TAATACG	360	0.0	42.500004	4
TCGTTAG	165	0.0	42.272728	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content