##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934123.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	883546
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.729815991470733	31.0	31.0	34.0	30.0	34.0
2	31.824309090867935	33.0	31.0	34.0	30.0	34.0
3	31.928017330167304	33.0	31.0	34.0	30.0	34.0
4	35.53921470981703	37.0	35.0	37.0	33.0	37.0
5	29.38790962779527	35.0	35.0	37.0	0.0	37.0
6	23.584399680378837	35.0	10.0	37.0	0.0	37.0
7	30.29316639993843	35.0	19.0	37.0	17.0	37.0
8	33.77849936505852	35.0	32.0	37.0	30.0	37.0
9	36.62259350390359	37.0	35.0	39.0	33.0	39.0
10	36.846054421614724	38.0	37.0	39.0	33.0	39.0
11	37.03439096549585	39.0	37.0	39.0	34.0	39.0
12	37.034227985866046	39.0	37.0	39.0	34.0	39.0
13	36.92639772009607	39.0	37.0	39.0	33.0	39.0
14	37.990436264778516	40.0	37.0	41.0	33.0	41.0
15	37.7828171934455	40.0	37.0	41.0	33.0	41.0
16	38.074514513109676	40.0	37.0	41.0	33.0	41.0
17	38.0233219323046	40.0	37.0	41.0	33.0	41.0
18	38.07318238099658	40.0	37.0	41.0	33.0	41.0
19	38.11685299916473	40.0	37.0	41.0	34.0	41.0
20	37.98149841660762	40.0	37.0	41.0	33.0	41.0
21	37.953416120948994	40.0	37.0	41.0	33.0	41.0
22	38.04439949929036	40.0	37.0	41.0	34.0	41.0
23	37.94963476717681	40.0	36.0	41.0	34.0	41.0
24	37.9432729025993	40.0	36.0	41.0	34.0	41.0
25	37.75502690295695	39.0	36.0	41.0	33.0	41.0
26	37.658128722217064	39.0	36.0	41.0	33.0	41.0
27	36.767598970512005	39.0	36.0	41.0	32.0	41.0
28	37.17753008898235	39.0	36.0	41.0	32.0	41.0
29	37.523518866023956	39.0	36.0	41.0	32.0	41.0
30	37.52606315913376	39.0	36.0	41.0	33.0	41.0
31	37.61774372811376	39.0	36.0	41.0	33.0	41.0
32	37.56368429034821	40.0	36.0	41.0	33.0	41.0
33	36.40196209365443	39.0	35.0	41.0	31.0	41.0
34	36.87028519171611	40.0	35.0	41.0	31.0	41.0
35	37.20620544940501	40.0	35.0	41.0	31.0	41.0
36	37.326313513954	40.0	35.0	41.0	33.0	41.0
37	37.420690037643766	40.0	36.0	41.0	33.0	41.0
38	37.43147159287688	40.0	36.0	41.0	33.0	41.0
39	37.431136579193385	40.0	36.0	41.0	33.0	41.0
40	37.39666412388263	39.0	35.0	41.0	33.0	41.0
41	37.24953199946579	39.0	35.0	41.0	32.0	41.0
42	37.3577617916894	39.0	35.0	41.0	33.0	41.0
43	37.25849361549936	39.0	35.0	41.0	33.0	41.0
44	37.30711134451404	39.0	35.0	41.0	33.0	41.0
45	37.27571173430699	39.0	35.0	41.0	33.0	41.0
46	37.21186785973792	39.0	35.0	41.0	33.0	41.0
47	37.09238907764848	39.0	35.0	40.0	33.0	41.0
48	37.10078931940159	39.0	35.0	40.0	33.0	41.0
49	37.21615060223237	39.0	35.0	40.0	33.0	41.0
50	37.12769906716798	39.0	35.0	40.0	33.0	41.0
51	36.45924603812365	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	3.0
10	12.0
11	3.0
12	8.0
13	11.0
14	9.0
15	22.0
16	20.0
17	48.0
18	92.0
19	199.0
20	403.0
21	866.0
22	1540.0
23	2274.0
24	3032.0
25	3776.0
26	4548.0
27	5601.0
28	7249.0
29	9791.0
30	13874.0
31	20309.0
32	29537.0
33	47549.0
34	64380.0
35	82394.0
36	105048.0
37	155766.0
38	174741.0
39	150385.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.69648212996267	4.170467638357255	47.72937685191263	16.403673379767437
2	18.7316789391837	12.926095528699127	46.55467853399823	21.78754699811894
3	20.242862284476416	12.890783275573655	47.19833489144877	19.668019548501153
4	18.791551317079133	4.091128249123418	53.042399603416236	24.074920830381213
5	18.818375047818677	22.178698109662655	37.295737856319874	21.707188986198794
6	15.258515119756074	33.86139487927057	37.447965357774244	13.432124643199108
7	74.51609763385268	2.1008527003687414	17.369554046987933	6.013495618790646
8	72.747655470117	8.178408368098548	10.88047481398818	8.193461347796266
9	67.45704241771226	5.3948521073039775	12.785978319182023	14.362127155801735
10	38.74082390730081	25.409769270643523	19.845938977710272	16.003467844345398
11	30.17443347601596	22.30478096216835	26.76917783567579	20.751607726139895
12	25.717053781014233	18.439447408510706	31.94887419557103	23.894624614904032
13	24.53828097235458	21.38439877493645	34.92200745631806	19.155312796390906
14	20.073770918548668	26.954227623689086	29.330108449362	23.641893008400242
15	16.59732487046515	23.381125600704433	38.24045380772478	21.781095721105636
16	18.3637297888282	23.428095424573254	30.46711772788287	27.741057058715672
17	18.58103596190804	23.71874243106754	31.77819830546457	25.92202330155985
18	18.574697865193208	23.17208158941357	32.24472749579535	26.00849304959787
19	20.62620395542507	24.637313733523776	29.705187958521684	25.031294352529464
20	23.373882061601773	25.788017828160616	32.11547559493224	18.722624515305373
21	22.986352719609393	28.17102901263771	28.91700035991335	19.925617907839545
22	19.145352930124748	23.91601569131658	29.84485244684487	27.093778931713796
23	19.859294252930802	27.295918944797442	28.58391074148941	24.260876060782348
24	23.305860702215845	22.82099630353145	27.473498833111123	26.399644161141584
25	19.528581420774923	27.929162714787914	26.03316635466631	26.509089509770856
26	18.975016580913728	23.74805612837362	30.863022411962703	26.413904878749943
27	22.656205788945908	26.585373030945757	27.006743282183383	23.751677897924953
28	16.902572135463238	23.103381148236764	33.044346304550075	26.949700411749927
29	20.2575757232787	20.565199774544844	32.82024931356149	26.356975188614967
30	23.24598832432041	23.363695834738653	28.81649625486392	24.57381958607701
31	20.913342372666506	22.594635706573285	26.17034087642296	30.32168104433725
32	21.492599140282454	24.915284546588406	29.964597202635744	23.6275191104934
33	20.416028141149415	22.899769791272895	26.325397885339303	30.35880418223839
34	19.93591731500114	20.001222347223575	31.29695567633151	28.765904661443777
35	19.46599271571599	18.877681524221714	33.81793364465461	27.838392115407686
36	19.919279811124717	21.009771986970684	32.40487761814325	26.666070583761343
37	20.74391146584332	21.555074665042905	33.08599665439038	24.615017214723398
38	18.96856530390042	22.166361457128435	28.471862245995116	30.393210992976034
39	19.85703064696122	20.00778680453536	33.23562100897973	26.899561539523692
40	19.692466492972635	18.737451134406133	32.56864951004248	29.001432862578746
41	17.47832031382633	21.599554522345183	30.592634678896175	30.329490484932307
42	19.36752585603919	19.277547518748317	35.85540537787506	25.49952124733743
43	21.929814633307153	18.816111441849095	32.670172237778225	26.58390168706553
44	20.219886683885164	18.55489131295937	31.1601206954703	30.065101307685165
45	20.314505413413674	16.72318136237389	29.327731663093942	33.634581561118495
46	24.152222974242427	19.411552992147552	31.10941592175167	25.32680811185835
47	15.841280476624872	20.14360316271026	37.517005339846484	26.49811102081838
48	17.888598895813008	19.79025427085856	32.50696624737139	29.81418058595704
49	19.3041448888909	16.05258809388532	38.54479563033504	26.098471386888743
50	20.79665348493457	16.384093188130556	32.63938719659191	30.17986613034296
51	18.06957419308106	16.72578450923891	29.442949206945652	35.76169209073438
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	142.0
1	483.5
2	825.0
3	1552.0
4	2279.0
5	1684.0
6	1089.0
7	1117.0
8	1145.0
9	1178.0
10	1211.0
11	1288.5
12	1366.0
13	1463.5
14	1561.0
15	1506.5
16	1452.0
17	1448.5
18	1445.0
19	1484.5
20	1524.0
21	1763.0
22	2002.0
23	2403.5
24	2805.0
25	3458.0
26	4829.5
27	5548.0
28	6333.5
29	7119.0
30	8651.0
31	10183.0
32	11685.0
33	13187.0
34	14524.5
35	15862.0
36	17661.0
37	19460.0
38	21438.0
39	23416.0
40	27709.0
41	32002.0
42	38562.0
43	45122.0
44	56269.0
45	67416.0
46	102364.5
47	137313.0
48	129255.0
49	121197.0
50	112304.0
51	103411.0
52	86117.5
53	68824.0
54	58416.5
55	48009.0
56	42511.0
57	37013.0
58	34323.5
59	31634.0
60	28401.5
61	25169.0
62	21681.0
63	18193.0
64	15008.0
65	11823.0
66	9368.0
67	6913.0
68	5615.0
69	4317.0
70	3642.0
71	2967.0
72	2441.5
73	1916.0
74	1581.5
75	984.0
76	721.0
77	527.0
78	333.0
79	247.5
80	162.0
81	129.5
82	97.0
83	54.5
84	12.0
85	6.5
86	1.0
87	1.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	883546.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.61133526181797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.68646654322391	25.8792592508725
2	12.123211022987542	8.876938864247283
3	4.989213954770035	5.479853543730793
4	2.6480999867134085	3.878019056815212
5	1.6642587867486027	3.0465368202039755
6	1.2325449132953938	2.7075069027543615
7	0.9403609224691661	2.409950829974174
8	0.7472518767622995	2.1886311188133787
9	0.619647661434802	2.0417515449291908
>10	4.185940137502716	26.47052752779188
>50	0.0999585091650529	2.527286347997987
>100	0.04997922172976824	3.4386002186056537
>500	0.0062065700187225025	1.5790149793483101
>1k	0.005879908438789739	5.544192714733693
>5k	3.266615799327632E-4	1.1227594202925044
>10k+	6.533231598655264E-4	2.809170858889075
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC	12894	1.4593467685892982	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG	10595	1.199145262385886	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	9388	1.0625366421216327	No Hit
CCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	4720	0.5342110088212725	TruSeq Adapter, Index 19 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	4456	0.5043314100227945	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCTCCTTT	4371	0.4947110846520724	No Hit
CTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	4241	0.4799976458497916	TruSeq Adapter, Index 16 (95% over 23bp)
GAATCAGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	3564	0.4033745837794523	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTC	3090	0.34972712230036695	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCT	2878	0.32573289902280134	No Hit
TCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	2762	0.31260398439922765	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTA	2402	0.2718590769467577	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	2355	0.2665396029182408	No Hit
GCTGTATCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1945	0.22013568054181673	TruSeq Adapter, Index 16 (95% over 22bp)
GAATATGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTC	1938	0.21934341845246313	No Hit
CCTGTATCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1635	0.18504978801330094	TruSeq Adapter, Index 16 (95% over 22bp)
CTGTCACTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGCT	1466	0.1659223175703359	TruSeq Adapter, Index 16 (95% over 23bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGC	1287	0.14566304414258002	No Hit
TCTGTATCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	1137	0.12868599937071754	TruSeq Adapter, Index 16 (95% over 22bp)
GAATAATACCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCG	1090	0.12336652534220063	No Hit
TCCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	1021	0.1155570847471439	TruSeq Adapter, Index 16 (95% over 21bp)
GAACTATCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCT	989	0.11193531519581323	No Hit
GCTGACTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTGC	956	0.1082003653460035	TruSeq Adapter, Index 19 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCTGCTCCTTTCGTATGCCGTCTTCTG	891	0.10084364594486307	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.263605969581663E-4	0.0	0.0	0.5087454416634788	0.0
2	2.263605969581663E-4	0.0	0.0	1.9711480783117121	0.0
3	2.263605969581663E-4	0.0	0.0	2.6260092853116874	0.0
4	2.263605969581663E-4	0.0	0.0	3.5504659632888385	0.0
5	2.263605969581663E-4	0.0	0.0	5.728620807518793	0.0
6	2.263605969581663E-4	0.0	0.0	6.561288263429408	0.0
7	2.263605969581663E-4	0.0	0.0	7.950576427260154	0.0
8	2.263605969581663E-4	0.0	0.0	9.938927910940686	0.0
9	2.263605969581663E-4	0.0	0.0	10.597863608685909	0.0
10	2.263605969581663E-4	0.0	0.0	13.447403983493786	0.0
11	2.263605969581663E-4	0.0	0.0	16.157845771470868	0.0
12	2.263605969581663E-4	0.0	0.0	20.479748649193137	0.0
13	2.263605969581663E-4	0.0	0.0	21.42016374925584	0.0
14	2.263605969581663E-4	0.0	0.0	21.79150830856571	0.0
15	2.263605969581663E-4	0.0	0.0	22.55581486419496	0.0
16	2.263605969581663E-4	0.0	0.0	23.642458909892635	0.0
17	2.263605969581663E-4	0.0	0.0	24.96191482956179	0.0
18	3.3954089543724946E-4	0.0	0.0	26.38877885248759	0.0
19	3.3954089543724946E-4	0.0	0.0	27.97273712970236	0.0
20	3.3954089543724946E-4	0.0	0.0	29.06277658435441	0.0
21	3.3954089543724946E-4	0.0	0.0	30.401359974466526	0.0
22	3.3954089543724946E-4	0.0	0.0	31.768917520989287	0.0
23	3.3954089543724946E-4	0.0	0.0	32.965120095614715	0.0
24	3.3954089543724946E-4	0.0	0.0	33.91357099686943	0.0
25	4.527211939163326E-4	0.0	0.0	34.759820088597536	0.0
26	4.527211939163326E-4	0.0	0.0	35.48417399886367	0.0
27	4.527211939163326E-4	0.0	0.0	36.22618403569254	0.0
28	4.527211939163326E-4	0.0	0.0	36.99139603370962	0.0
29	4.527211939163326E-4	0.0	0.0	37.81229273857841	0.0
30	4.527211939163326E-4	0.0	0.0	38.658994551500435	0.0
31	4.527211939163326E-4	0.0	0.0	39.4648382766715	0.0
32	4.527211939163326E-4	0.0	0.0	40.254836760055504	0.0
33	4.527211939163326E-4	0.0	0.0	40.968099001070684	0.0
34	4.527211939163326E-4	0.0	0.0	41.700035991334914	0.0
35	4.527211939163326E-4	0.0	0.0	42.42880393324173	0.0
36	4.527211939163326E-4	0.0	0.0	43.16979534738429	0.0
37	4.527211939163326E-4	0.0	0.0	43.92765062600023	0.0
38	4.527211939163326E-4	0.0	0.0	44.66230394342796	0.0
39	4.527211939163326E-4	0.0	0.0	45.42932682622071	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGACA	30	2.1649303E-6	45.000004	30
CATACGC	30	2.1649303E-6	45.000004	32
CGTCAGG	30	2.1649303E-6	45.000004	2
AACGTAC	20	7.0324005E-4	45.0	37
AAGTACG	20	7.0324005E-4	45.0	10
CGCGCAT	25	3.8900707E-5	45.0	36
CGCGAAC	20	7.0324005E-4	45.0	11
CCCGTAA	20	7.0324005E-4	45.0	36
GGACGAT	20	7.0324005E-4	45.0	8
CGATCGA	25	3.8900707E-5	45.0	41
GGTTACG	20	7.0324005E-4	45.0	1
TCGAATA	20	7.0324005E-4	45.0	44
CCGTTAG	25	3.8900707E-5	45.0	1
CGAAGTT	20	7.0324005E-4	45.0	23
CGTTCGG	65	0.0	44.999996	2
AATACGG	345	0.0	44.347824	5
TCGTTTG	200	0.0	42.75	1
AGGGATT	550	0.0	42.545452	6
TAATACG	360	0.0	42.500004	4
TCGTTAG	165	0.0	42.272728	1
>>END_MODULE