##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934120.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	389009
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.96194432519556	33.0	31.0	34.0	30.0	34.0
2	32.1915688325977	33.0	31.0	34.0	30.0	34.0
3	32.25997341963811	34.0	31.0	34.0	30.0	34.0
4	35.690122850628136	37.0	35.0	37.0	33.0	37.0
5	29.45955234968857	37.0	35.0	37.0	0.0	37.0
6	23.678539571063908	35.0	10.0	37.0	0.0	37.0
7	30.40744558609184	35.0	19.0	37.0	17.0	37.0
8	34.290887871488835	35.0	32.0	37.0	32.0	37.0
9	36.91687595916804	38.0	37.0	39.0	34.0	39.0
10	36.984766419285926	39.0	37.0	39.0	34.0	39.0
11	37.177427771593976	39.0	37.0	39.0	34.0	39.0
12	37.166903593490126	39.0	37.0	39.0	34.0	39.0
13	37.07070016374942	39.0	37.0	39.0	33.0	39.0
14	38.19973316812722	40.0	37.0	41.0	33.0	41.0
15	38.076748866992794	40.0	37.0	41.0	33.0	41.0
16	38.272862581585514	40.0	37.0	41.0	34.0	41.0
17	38.27226362372079	40.0	37.0	41.0	34.0	41.0
18	38.36337976756322	40.0	37.0	41.0	34.0	41.0
19	38.383047692983965	40.0	37.0	41.0	34.0	41.0
20	38.34304604777782	40.0	37.0	41.0	34.0	41.0
21	38.229264618556385	40.0	37.0	41.0	34.0	41.0
22	38.29305234583261	40.0	37.0	41.0	34.0	41.0
23	38.24621795382626	40.0	37.0	41.0	34.0	41.0
24	38.213681431535	40.0	37.0	41.0	34.0	41.0
25	37.98143487682804	40.0	37.0	41.0	34.0	41.0
26	37.90141359197345	40.0	37.0	41.0	34.0	41.0
27	37.05254377148086	40.0	36.0	41.0	33.0	41.0
28	37.41634512312054	40.0	36.0	41.0	33.0	41.0
29	37.72038693192189	40.0	36.0	41.0	33.0	41.0
30	37.712986074872305	40.0	36.0	41.0	33.0	41.0
31	37.771282926616095	40.0	36.0	41.0	33.0	41.0
32	37.754483829422966	40.0	36.0	41.0	33.0	41.0
33	36.50490348552347	40.0	35.0	41.0	31.0	41.0
34	37.01460120459938	40.0	35.0	41.0	31.0	41.0
35	37.354798989226474	40.0	35.0	41.0	32.0	41.0
36	37.4649995244326	40.0	35.0	41.0	33.0	41.0
37	37.60359271893452	40.0	36.0	41.0	33.0	41.0
38	37.537136672930444	40.0	36.0	41.0	33.0	41.0
39	37.54395656655758	40.0	36.0	41.0	33.0	41.0
40	37.5003894511438	40.0	36.0	41.0	33.0	41.0
41	37.42767648049274	40.0	35.0	41.0	33.0	41.0
42	37.468945448562884	40.0	35.0	41.0	33.0	41.0
43	37.34006154099262	39.0	35.0	41.0	33.0	41.0
44	37.37265461724536	39.0	35.0	41.0	33.0	41.0
45	37.30948641291076	39.0	35.0	41.0	33.0	41.0
46	37.24886570747721	39.0	35.0	41.0	33.0	41.0
47	37.206722723638784	39.0	35.0	41.0	33.0	41.0
48	37.16374942482051	39.0	35.0	41.0	33.0	41.0
49	37.194003223575805	39.0	35.0	41.0	33.0	41.0
50	37.109807228110405	39.0	35.0	41.0	33.0	41.0
51	36.35075281034629	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	4.0
12	4.0
13	5.0
14	8.0
15	13.0
16	12.0
17	30.0
18	49.0
19	71.0
20	162.0
21	237.0
22	403.0
23	668.0
24	959.0
25	1454.0
26	1972.0
27	2451.0
28	3114.0
29	4112.0
30	5725.0
31	8111.0
32	11834.0
33	19512.0
34	26938.0
35	35128.0
36	44324.0
37	67348.0
38	81122.0
39	73186.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.303316889840595	5.0096527329701885	53.02910729571809	18.65792308147112
2	19.059456207954057	4.328434560639985	54.6128752805205	21.999233950885454
3	20.616232529324513	4.46827708356362	53.006742774588766	21.9087476125231
4	19.44119544792023	4.641794919911878	51.42117534555756	24.495834286610336
5	15.107619618055109	22.55166332912606	41.313954175867394	21.026762876951434
6	16.2885691590683	29.235313321799755	40.12066558871389	14.355451930418061
7	82.68523350359503	2.1793840245341367	9.802600968100995	5.332781503769836
8	83.18367955497173	2.476035258824346	8.09929847381423	6.240986712389688
9	77.52082856694832	5.127130734764491	10.926225357253944	6.425815341033241
10	37.485765110832915	29.38183949471606	17.863082859265468	15.269312535185561
11	27.664141446598922	25.433087666352193	25.913025148518415	20.98974573853047
12	25.906598561987	21.039616050014267	30.41317810127786	22.640607286720872
13	22.405908346593524	24.900966301550863	31.60723787881514	21.08588747304047
14	19.53322416705012	26.380880648005572	30.68129529136858	23.40459989357573
15	18.350475181808132	24.453418815502985	34.417712700734434	22.778393301954452
16	20.08076933952685	24.708168705608355	32.388967864496706	22.82209409036809
17	19.412404340259478	24.219234002298148	29.050741756617455	27.31761990082492
18	20.933191777054002	22.798958378854987	32.09077424943896	24.17707559465205
19	22.353210337035904	24.405090884786727	30.089278140094443	23.152420638082923
20	24.075278463994408	25.03772406293942	30.285160497572033	20.60183697549414
21	23.82181389119532	26.20607749435103	28.814500435722568	21.15760817873108
22	22.036251089306415	24.948265978422093	28.023259101974507	24.992223830296986
23	21.2198175363552	25.593495266176365	27.407592112264755	25.779095085203686
24	22.55166332912606	23.202805076489234	29.49391916382397	24.751612430560733
25	20.103647987578693	25.233863483878267	27.61555645242141	27.04693207612163
26	18.964856854211597	25.867524915875983	28.45975285918835	26.707865370724072
27	19.560215830482072	26.819687976370727	29.44173528118887	24.178360911958336
28	17.816554372778008	24.014097360215317	33.01620270996301	25.153145557043665
29	20.574074121678418	22.07686711618497	31.72908595945081	25.619972802685798
30	20.86918297520109	23.508453531923426	32.1154523417196	23.506911151155883
31	22.59973419638106	25.33643180491968	27.865165073301647	24.198668925397612
32	23.76860175471519	24.781431792066506	27.305023791223338	24.144942661994968
33	21.820317781850807	27.729178502296865	27.463889010279967	22.986614705572364
34	20.20544511823634	23.802277068139812	29.423740838900898	26.56853697472295
35	20.746306640720395	22.70127426357745	28.40114239002183	28.151276705680335
36	21.152723972967205	24.5464757884779	30.590037762622458	23.710762475932434
37	20.728569261893686	24.89274027079065	32.19180018971283	22.186890277602835
38	21.772246914595804	25.52717289317214	29.170019202640557	23.5305609895915
39	22.08869203540278	23.256274276430624	30.737078062461283	23.917955625705318
40	22.460919927302452	21.87095928371837	29.724762152032476	25.9433586369467
41	20.046836962640967	23.31205704752332	27.73920397728587	28.90190201254984
42	21.58304820711089	23.475806472343827	28.032513386579744	26.908631933965538
43	21.338580855455785	23.411797670490913	30.20572788804372	25.04389358600958
44	20.12781195293682	22.459634609996172	29.81550555385608	27.59704788321093
45	21.116220961468755	21.794097308802623	28.726841795434037	28.36283993429458
46	22.03830759699647	22.112341873838396	30.748902981679088	25.10044754748605
47	18.845579408188502	22.49510936764959	32.55477379700727	26.10453742715464
48	19.676151451508833	22.319020896688766	32.08306234560126	25.921765306201145
49	21.626491932063267	21.019050973113732	32.83008876401317	24.524368330809825
50	21.13087357876039	20.301586852746336	31.48693217894702	27.08060738954626
51	20.15120472791118	20.215470593225348	29.148939998817507	30.484384680045963
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	61.0
1	229.0
2	397.0
3	711.0
4	1025.0
5	759.0
6	493.0
7	531.0
8	569.0
9	566.0
10	563.0
11	602.0
12	641.0
13	656.0
14	671.0
15	697.0
16	723.0
17	665.0
18	607.0
19	666.5
20	726.0
21	960.0
22	1194.0
23	1341.0
24	1488.0
25	2018.0
26	2987.0
27	3426.0
28	3933.5
29	4441.0
30	5136.0
31	5831.0
32	6597.0
33	7363.0
34	8667.5
35	9972.0
36	10712.0
37	11452.0
38	12561.5
39	13671.0
40	15828.5
41	17986.0
42	20842.0
43	23698.0
44	26382.0
45	29066.0
46	34126.5
47	39187.0
48	43232.5
49	47278.0
50	45035.0
51	42792.0
52	35868.0
53	28944.0
54	25525.0
55	22106.0
56	19584.0
57	17062.0
58	15616.0
59	14170.0
60	13002.5
61	11835.0
62	10516.5
63	9198.0
64	7811.5
65	6425.0
66	5293.5
67	4162.0
68	3591.5
69	3021.0
70	2496.5
71	1972.0
72	1566.5
73	1161.0
74	882.0
75	445.0
76	287.0
77	204.5
78	122.0
79	89.5
80	57.0
81	35.5
82	14.0
83	7.5
84	1.0
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	389009.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	46.41987913789423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.78669410480775	34.25169422329948
2	10.369179121902581	9.62672083195788
3	4.147777636169481	5.7761800968554375
4	2.4815786768095047	4.607783289946908
5	1.7979982171055509	4.173142996409449
6	1.3589641829165038	3.784977187422678
7	1.0604177740780172	3.445712543586346
8	0.8360791167892595	3.1048553240859817
9	0.6562671006810896	2.74174555462132
>10	3.439896560549532	23.7249504130974
>50	0.039874767944324346	1.2748312018572658
>100	0.02190304154688235	1.9039043491132113
>500	0.0016848493497601809	0.5339170350518768
>1k	0.0016848493497601809	1.0495849526947538
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1728	0.44420566105154385	No Hit
CTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT	1293	0.33238305540488783	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	1005	0.25834877856296384	No Hit
GCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	927	0.23829782858494275	No Hit
CCTGTATCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	600	0.15423807675400825	No Hit
CTGTCACTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGCT	521	0.1339300633147305	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTATCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTGC	403	0.10359657488644221	No Hit
TCCTGTCTCTTATACACATCTGACGCCTTGTCGATCGTATGCCGTCTTCTG	393	0.10102594027387542	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3598888457593526	0.0
2	0.0	0.0	0.0	1.3842867388672242	0.0
3	0.0	0.0	0.0	1.912809215210959	0.0
4	0.0	0.0	0.0	2.4557272453850683	0.0
5	0.0	0.0	0.0	3.407633242418556	0.0
6	0.0	0.0	0.0	4.067258084003198	0.0
7	0.0	0.0	0.0	4.944615677272248	0.0
8	0.0	0.0	0.0	6.35923590456776	0.0
9	0.0	0.0	0.0	6.9450835327717355	0.0
10	0.0	0.0	0.0	8.120120614176022	0.0
11	0.0	0.0	0.0	10.303617654090266	0.0
12	0.0	0.0	0.0	12.307941461508603	0.0
13	0.0	0.0	0.0	12.964995668480677	0.0
14	0.0	0.0	0.0	13.186070245161423	0.0
15	0.0	0.0	0.0	13.528221712094064	0.0
16	0.0	0.0	0.0	14.319720109303384	0.0
17	0.0	0.0	0.0	15.41275394656679	0.0
18	0.0	0.0	0.0	16.648972131750167	0.0
19	0.0	0.0	0.0	17.421961959749	0.0
20	0.0	0.0	0.0	18.25613289152693	0.0
21	2.5706346125668045E-4	0.0	0.0	19.42320100563226	0.0
22	2.5706346125668045E-4	0.0	0.0	20.540141744792535	0.0
23	2.5706346125668045E-4	0.0	0.0	21.620579472454367	0.0
24	2.5706346125668045E-4	0.0	0.0	22.470945402291463	0.0
25	2.5706346125668045E-4	0.0	0.0	23.251390070666744	0.0
26	2.5706346125668045E-4	0.0	0.0	23.951373875668686	0.0
27	2.5706346125668045E-4	0.0	0.0	24.611769907637097	0.0
28	2.5706346125668045E-4	0.0	0.0	25.297101095347408	0.0
29	2.5706346125668045E-4	0.0	0.0	26.03024608685146	0.0
30	2.5706346125668045E-4	0.0	0.0	26.839738926348748	0.0
31	2.5706346125668045E-4	0.0	0.0	27.558488364022427	0.0
32	2.5706346125668045E-4	0.0	0.0	28.266441136323323	0.0
33	2.5706346125668045E-4	0.0	0.0	28.95897010094882	0.0
34	2.5706346125668045E-4	0.0	0.0	29.65381263672563	0.0
35	2.5706346125668045E-4	0.0	0.0	30.41497754550666	0.0
36	2.5706346125668045E-4	0.0	0.0	31.14735134662694	0.0
37	2.5706346125668045E-4	0.0	0.0	31.822399995886986	0.0
38	2.5706346125668045E-4	0.0	0.0	32.540121179715634	0.0
39	2.5706346125668045E-4	0.0	0.0	33.28174926544116	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTACG	30	2.1617852E-6	45.000004	7
TACGAAT	50	2.1827873E-11	45.000004	12
CGCAGTA	25	3.8860308E-5	45.000004	37
CCAACCG	25	3.8860308E-5	45.000004	32
CGTCATA	25	3.8860308E-5	45.000004	38
TCGTTAG	40	6.7957444E-9	45.0	1
CTGTACG	20	7.027526E-4	45.0	1
GTCTACG	20	7.027526E-4	45.0	1
ACGGGTA	35	1.209155E-7	45.0	5
CATTAGT	20	7.027526E-4	45.0	38
CGTTAGG	70	0.0	45.0	2
TGTAGCG	20	7.027526E-4	45.0	2
ATTGCGG	20	7.027526E-4	45.0	2
CGTTTGG	145	0.0	43.44828	2
CGTTATT	90	0.0	42.5	1
TTTCACG	85	0.0	42.35294	1
TAGGGAC	355	0.0	41.830986	5
TTATGGG	1160	0.0	41.31466	3
CGTGGGT	60	3.6379788E-12	41.250004	4
GGATGAC	115	0.0	41.086956	8
>>END_MODULE