##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934119.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	411230
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.800393940130828	31.0	31.0	34.0	30.0	34.0
2	31.98830338253532	33.0	31.0	34.0	30.0	34.0
3	32.07095056294531	33.0	31.0	34.0	30.0	34.0
4	35.59468667169224	37.0	35.0	37.0	33.0	37.0
5	29.9357926221336	37.0	35.0	37.0	0.0	37.0
6	24.161184738467526	35.0	17.0	37.0	0.0	37.0
7	30.513102156943802	35.0	19.0	37.0	17.0	37.0
8	34.09238382413734	35.0	32.0	37.0	32.0	37.0
9	36.704746735403546	37.0	35.0	39.0	33.0	39.0
10	36.836463779393526	38.0	37.0	39.0	33.0	39.0
11	37.0300707633198	39.0	37.0	39.0	34.0	39.0
12	37.01663545947523	39.0	37.0	39.0	33.0	39.0
13	36.90737543467159	39.0	37.0	39.0	33.0	39.0
14	37.99046275806726	40.0	37.0	41.0	33.0	41.0
15	37.838328915691946	40.0	37.0	41.0	33.0	41.0
16	38.07834788317973	40.0	37.0	41.0	33.0	41.0
17	38.080869586362866	40.0	37.0	41.0	34.0	41.0
18	38.16185589572745	40.0	37.0	41.0	34.0	41.0
19	38.18469226466941	40.0	37.0	41.0	34.0	41.0
20	38.10268462903971	40.0	37.0	41.0	34.0	41.0
21	37.99212119738346	40.0	37.0	41.0	34.0	41.0
22	38.05509082508572	40.0	37.0	41.0	34.0	41.0
23	37.98566495635046	40.0	37.0	41.0	34.0	41.0
24	37.97857160226637	40.0	37.0	41.0	34.0	41.0
25	37.721331128565524	39.0	36.0	41.0	33.0	41.0
26	37.63714223184106	39.0	36.0	41.0	33.0	41.0
27	36.85422512948958	39.0	36.0	41.0	32.0	41.0
28	37.22560854023296	39.0	36.0	41.0	32.0	41.0
29	37.44250419473288	39.0	36.0	41.0	32.0	41.0
30	37.39186343408798	39.0	36.0	41.0	32.0	41.0
31	37.433263623762855	39.0	36.0	41.0	33.0	41.0
32	37.394900663862074	39.0	36.0	40.0	33.0	41.0
33	36.294450793959584	39.0	35.0	40.0	31.0	41.0
34	36.80185054592321	39.0	35.0	41.0	31.0	41.0
35	37.050324635848554	39.0	35.0	41.0	31.0	41.0
36	37.16505848308732	39.0	35.0	40.0	32.0	41.0
37	37.246866716922405	39.0	35.0	40.0	33.0	41.0
38	37.09646669746857	39.0	35.0	40.0	32.0	41.0
39	37.07034263064465	39.0	35.0	40.0	32.0	41.0
40	37.07870777910172	39.0	35.0	40.0	32.0	41.0
41	37.05098120273326	39.0	35.0	40.0	32.0	41.0
42	37.12230625197578	39.0	35.0	40.0	32.0	41.0
43	36.98615373392019	39.0	35.0	40.0	32.0	41.0
44	36.96449432191231	39.0	35.0	40.0	32.0	41.0
45	36.84872212630402	39.0	35.0	40.0	32.0	41.0
46	36.769681200301534	39.0	35.0	40.0	32.0	41.0
47	36.669540160007784	39.0	35.0	40.0	31.0	41.0
48	36.56612601220728	39.0	35.0	40.0	31.0	41.0
49	36.542423947669185	38.0	35.0	40.0	32.0	41.0
50	36.36676555698757	38.0	35.0	40.0	32.0	41.0
51	35.533137173844324	37.0	34.0	39.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	8.0
11	5.0
12	4.0
13	11.0
14	7.0
15	10.0
16	20.0
17	38.0
18	58.0
19	107.0
20	186.0
21	342.0
22	536.0
23	824.0
24	1159.0
25	1724.0
26	2284.0
27	2818.0
28	3697.0
29	4966.0
30	6828.0
31	9982.0
32	14295.0
33	22344.0
34	31067.0
35	40708.0
36	50754.0
37	74490.0
38	81585.0
39	60329.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.98122705055565	5.174719743209396	52.15037813389101	18.693675072343943
2	20.04790506529193	7.304671351798264	50.55589329572258	22.09153028718722
3	19.62843177783722	6.823918488437128	51.06072027818982	22.48692945553583
4	19.6218661089901	4.529581985750067	50.11793886632785	25.73061303893198
5	17.216642754662843	20.93281132213117	39.623568319431946	22.226977603774042
6	15.788731366874984	30.252413491233614	39.56836806653211	14.390487075359287
7	78.3291588648688	2.7821413807358413	13.155168640420204	5.733531113975148
8	77.20205237944703	4.471463657807067	10.164628067018457	8.161855895727452
9	70.97171898937334	5.993969311577462	13.415850010942782	9.618461688106413
10	37.17116941857355	24.95926853585585	20.61133672154269	17.258225324027915
11	27.6558130486589	23.010237579943098	27.716119932884276	21.617829438513727
12	23.962502735695352	19.578824502103444	34.14877319261727	22.309899569583933
13	22.378960678938796	23.24684483135958	34.97191352770955	19.402280961992073
14	18.834715366096834	27.094569948690513	32.14065121708047	21.93006346813219
15	16.600199401794615	23.78863409770688	37.923303260948856	21.687863239549642
16	17.749434622960386	23.563699146463048	32.81351068744985	25.87335554312672
17	17.531065340563675	23.533545704350363	30.522335432726212	28.41305352235975
18	19.608005252535076	22.316222065510786	33.17997227828709	24.89580040366705
19	20.31685431510347	24.55633100697906	30.21885562823724	24.90795904968023
20	22.751501592782628	25.369501252340537	31.36590229312064	20.513094861756194
21	22.704812392092016	25.641611750115505	30.642462855336426	21.011113002456046
22	20.19721323833378	23.774773241251854	29.857987014566056	26.17002650584831
23	19.144517666512655	25.383848454636095	28.891861002358777	26.579772876492473
24	22.37312452885247	22.60851591566763	29.408360284998665	25.609999270481236
25	19.38185443668993	25.89499793303018	27.304428178878	27.41871945140189
26	20.32293363811006	25.5100552002529	28.933929917564384	25.233081244072658
27	20.271137806093915	26.439948447340907	28.605160129367995	24.683753617197187
28	19.594387569000315	23.490990443304234	31.486516061571386	25.42810592612407
29	20.771830848916665	23.895630182622863	29.900055929771664	25.432483038688815
30	21.88823772584685	26.151059018067745	27.36984169442891	24.590861561656492
31	23.354570435036354	26.685309923886873	25.741555820343848	24.21856382073292
32	21.773216934562168	29.132115847579215	24.002383094618583	25.092284123240038
33	20.13155654986261	29.61165284633903	24.232424677187947	26.02436592661041
34	21.60469810081949	24.95002796488583	28.48673491719962	24.958539017095056
35	19.93239792816672	24.942246431437397	26.008073340952752	29.117282299443133
36	23.712277800744108	26.03652457262359	25.65401356904895	24.597184057583345
37	20.810738516158843	26.153247574350118	28.683704982613133	24.352308926877903
38	20.082678792889624	29.91610534250906	24.671594971184007	25.32962089341731
39	19.807650220071494	29.65493762614595	28.295844174792695	22.24156797898986
40	21.345962113659024	25.241349123361623	29.517301753276755	23.8953870097026
41	18.961894803394692	24.114972156700627	27.913089998297792	29.010043041606888
42	21.62682683656348	23.109938477251173	26.38669357780318	28.87654110838217
43	23.656834374924006	24.78904749167133	25.70921382194879	25.844904311455874
44	21.750845025897917	23.75215815966734	27.484619312793328	27.012377501641417
45	21.91036646159084	22.76049899083238	26.69625270529874	28.632881842278046
46	23.918488437127642	23.127690100430414	28.734528122948227	24.219293339493714
47	18.092551613452326	23.96007100649272	32.41349123361623	25.533886146438732
48	18.96505605135812	23.95812562313061	29.24543442842205	27.831383897089218
49	20.5177151472412	21.857597937893637	32.266371616856745	25.358315298008417
50	21.161150694258687	21.23021180361355	29.803029934586483	27.80560756754128
51	18.93392991756438	21.707560246090996	26.608953626924105	32.74955620942052
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	138.0
1	329.0
2	520.0
3	845.5
4	1171.0
5	860.0
6	549.0
7	562.5
8	576.0
9	650.0
10	724.0
11	789.5
12	855.0
13	840.5
14	826.0
15	836.0
16	846.0
17	822.0
18	798.0
19	860.5
20	923.0
21	1032.5
22	1142.0
23	1489.0
24	1836.0
25	1961.5
26	2360.5
27	2634.0
28	3305.5
29	3977.0
30	4556.0
31	5135.0
32	5892.0
33	6649.0
34	7398.5
35	8148.0
36	8649.5
37	9151.0
38	11095.5
39	13040.0
40	15124.5
41	17209.0
42	20654.5
43	24100.0
44	29173.5
45	34247.0
46	43138.0
47	52029.0
48	53920.5
49	55812.0
50	52730.0
51	49648.0
52	40777.0
53	31906.0
54	27179.0
55	22452.0
56	19254.0
57	16056.0
58	14287.0
59	12518.0
60	11181.0
61	9844.0
62	8906.0
63	7968.0
64	6889.0
65	5810.0
66	4600.0
67	3390.0
68	2873.0
69	2356.0
70	1963.5
71	1571.0
72	1382.0
73	1193.0
74	989.5
75	529.0
76	272.0
77	218.0
78	164.0
79	126.0
80	88.0
81	65.0
82	42.0
83	41.5
84	41.0
85	21.5
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	411230.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.175248101634324
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.47438172137416	31.290994118328815
2	11.70424487142936	10.106673523324874
3	4.715205279651579	6.107404733972791
4	2.5541296780358507	4.4110073013178095
5	1.6005784193820094	3.4552685181469975
6	1.1543915197122236	2.990468416199877
7	0.8774680054278098	2.651942918491433
8	0.6992008150081894	2.41505349286748
9	0.5817093247490958	2.2603899977168744
>10	3.5259412606095095	25.177298094784025
>50	0.06753499667608764	1.9788176795051973
>100	0.037201481219878756	3.1046285371257842
>500	0.004006313362140789	1.0833087795892582
>1k	0.004006313362140789	2.966743888628794
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	2789	0.6782092746151789	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGC	1953	0.4749167132748098	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCG	1736	0.42214818957760863	No Hit
GCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	1485	0.36111178659144516	No Hit
TCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	1459	0.3547892906645916	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC	1306	0.31758383386426087	No Hit
CTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGCT	1278	0.3107749920968801	No Hit
CCTGTATCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	970	0.23587773265569145	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCGAATCAT	692	0.1682756608224108	No Hit
CGCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTG	578	0.14055394791236048	No Hit
TCCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTG	574	0.1395812562313061	No Hit
TCTGTATCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	538	0.1308270311018165	No Hit
GCTGTATCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	531	0.1291248206599713	No Hit
CCTGACTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTCTGC	501	0.12182963305206332	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCT	495	0.12037059553048172	No Hit
GAATCAGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTCTTC	441	0.10723925783624735	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCGAATCATCGTATGCCGTC	430	0.10456435571334775	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.33387641952192204	0.0
2	0.0	0.0	0.0	1.8746200423120882	0.0
3	0.0	0.0	0.0	2.663229822726941	0.0
4	0.0	0.0	0.0	3.517496291612966	0.0
5	0.0	0.0	0.0	5.116601415266396	0.0
6	0.0	0.0	0.0	6.060355518809425	0.0
7	0.0	0.0	0.0	7.362303333900737	0.0
8	0.0	0.0	0.0	9.337353792281691	0.0
9	0.0	0.0	0.0	10.21958514699803	0.0
10	0.0	0.0	0.0	12.184179169807651	0.0
11	0.0	0.0	0.0	15.03927242662257	0.0
12	0.0	0.0	0.0	18.126109476448704	0.0
13	0.0	0.0	0.0	19.000072951876078	0.0
14	0.0	0.0	0.0	19.3312744692751	0.0
15	0.0	0.0	0.0	19.831967512097854	0.0
16	0.0	0.0	0.0	20.984120808306788	0.0
17	0.0	0.0	0.0	22.583469104880482	0.0
18	0.0	0.0	0.0	24.448848576222552	0.0
19	0.0	0.0	0.0	25.785326945991294	0.0
20	0.0	0.0	0.0	26.909029010529387	0.0
21	0.0	0.0	0.0	28.31456848965299	0.0
22	0.0	0.0	0.0	29.653235415704106	0.0
23	0.0	0.0	0.0	30.989956958393112	0.0
24	0.0	0.0	0.0	32.03049388420106	0.0
25	0.0	0.0	0.0	32.879167375921014	0.0
26	0.0	0.0	0.0	33.67337013350193	0.0
27	0.0	0.0	0.0	34.45492789922914	0.0
28	0.0	0.0	0.0	35.28682245945092	0.0
29	0.0	0.0	0.0	36.137927680373515	0.0
30	0.0	0.0	0.0	37.012863847481945	0.0
31	0.0	0.0	0.0	37.89947231476303	0.0
32	0.0	0.0	0.0	38.69537728278579	0.0
33	0.0	0.0	0.0	39.495902536293556	0.0
34	0.0	0.0	0.0	40.285485008389465	0.0
35	0.0	0.0	0.0	41.085280743136444	0.0
36	0.0	0.0	0.0	41.81528584976777	0.0
37	0.0	0.0	0.0	42.58006468399679	0.0
38	0.0	0.0	0.0	43.32538968460472	0.0
39	0.0	0.0	0.0	44.071687376893706	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATA	35	1.2093915E-7	45.000004	14
TACGGGT	35	1.2093915E-7	45.000004	4
TACGGCA	35	1.2093915E-7	45.000004	13
CGTTATT	35	1.2093915E-7	45.000004	1
CGGGCTT	35	1.2093915E-7	45.000004	6
TCATCGA	35	1.2093915E-7	45.000004	16
ACGAACG	35	1.2093915E-7	45.000004	21
CAGCGTT	25	3.8864207E-5	45.0	15
ACACGTG	25	3.8864207E-5	45.0	42
GATAACG	40	6.7975634E-9	45.0	17
TACCACG	20	7.027997E-4	45.0	21
CATTGTG	20	7.027997E-4	45.0	11
TTTCGAG	110	0.0	45.0	1
CGCATGG	25	3.8864207E-5	45.0	2
CCAGCGT	25	3.8864207E-5	45.0	14
ATTAACG	20	7.027997E-4	45.0	24
ACGGGTA	20	7.027997E-4	45.0	5
CGTTATG	20	7.027997E-4	45.0	1
ATACTAC	40	6.7975634E-9	45.0	3
GCCGAAT	20	7.027997E-4	45.0	30
>>END_MODULE