##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934118.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	460175
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.896926169392078	33.0	31.0	34.0	30.0	34.0
2	32.099088390286305	33.0	31.0	34.0	30.0	34.0
3	32.16727114684631	34.0	31.0	34.0	30.0	34.0
4	35.659455641875375	37.0	35.0	37.0	33.0	37.0
5	29.453499212256208	37.0	35.0	37.0	0.0	37.0
6	23.679428478296302	35.0	10.0	37.0	0.0	37.0
7	30.38093551366328	35.0	19.0	37.0	17.0	37.0
8	34.18281523333514	35.0	32.0	37.0	32.0	37.0
9	36.82475579942413	38.0	37.0	39.0	34.0	39.0
10	36.90814798717879	39.0	37.0	39.0	33.0	39.0
11	37.10707882870647	39.0	37.0	39.0	34.0	39.0
12	37.08492855978704	39.0	37.0	39.0	34.0	39.0
13	36.98464062584886	39.0	37.0	39.0	33.0	39.0
14	38.04873798011626	40.0	37.0	41.0	33.0	41.0
15	37.950236323137936	40.0	37.0	41.0	33.0	41.0
16	38.18655511490194	40.0	37.0	41.0	34.0	41.0
17	38.211302221980766	40.0	37.0	41.0	34.0	41.0
18	38.299779431737925	40.0	37.0	41.0	34.0	41.0
19	38.32516977236921	40.0	37.0	41.0	34.0	41.0
20	38.259853316673	40.0	37.0	41.0	34.0	41.0
21	38.155897213016786	40.0	37.0	41.0	34.0	41.0
22	38.21751507578639	40.0	37.0	41.0	34.0	41.0
23	38.17343401966643	40.0	37.0	41.0	34.0	41.0
24	38.146059651219645	40.0	37.0	41.0	34.0	41.0
25	37.88927038626609	40.0	36.0	41.0	34.0	41.0
26	37.76485467485196	40.0	36.0	41.0	33.0	41.0
27	36.95758135491933	40.0	36.0	41.0	32.0	41.0
28	37.35203781170207	40.0	36.0	41.0	33.0	41.0
29	37.644133210191775	40.0	36.0	41.0	33.0	41.0
30	37.64110827402619	39.0	36.0	41.0	33.0	41.0
31	37.69618297386864	39.0	36.0	41.0	33.0	41.0
32	37.69292551746618	40.0	36.0	41.0	33.0	41.0
33	36.518948226218285	39.0	35.0	41.0	31.0	41.0
34	37.03188352257293	40.0	35.0	41.0	32.0	41.0
35	37.34629217145651	40.0	35.0	41.0	32.0	41.0
36	37.49701961210409	40.0	36.0	41.0	33.0	41.0
37	37.61107187483023	40.0	36.0	41.0	33.0	41.0
38	37.5678839571902	40.0	36.0	41.0	33.0	41.0
39	37.59006247623187	40.0	36.0	41.0	33.0	41.0
40	37.53048514152225	40.0	36.0	41.0	33.0	41.0
41	37.49256695822242	40.0	36.0	41.0	33.0	41.0
42	37.55894388004563	40.0	36.0	41.0	33.0	41.0
43	37.472700603031456	40.0	36.0	41.0	33.0	41.0
44	37.4927929592003	40.0	36.0	41.0	33.0	41.0
45	37.41947085347965	39.0	35.0	41.0	33.0	41.0
46	37.342441462487095	39.0	35.0	41.0	33.0	41.0
47	37.33323626881078	39.0	35.0	41.0	33.0	41.0
48	37.328485902102464	39.0	35.0	41.0	33.0	41.0
49	37.39494974737872	39.0	35.0	41.0	33.0	41.0
50	37.31582767425436	39.0	35.0	41.0	33.0	41.0
51	36.5682707665562	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	2.0
11	2.0
12	1.0
13	6.0
14	7.0
15	19.0
16	8.0
17	29.0
18	46.0
19	109.0
20	175.0
21	254.0
22	472.0
23	732.0
24	1152.0
25	1525.0
26	2093.0
27	2647.0
28	3435.0
29	4740.0
30	6752.0
31	9657.0
32	14299.0
33	23504.0
34	32619.0
35	42736.0
36	53655.0
37	81149.0
38	95721.0
39	82561.0
40	64.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.132938555984136	5.1717281469006355	54.50122236105829	17.194110936056937
2	19.91329385559841	5.33079806595317	52.908350084207086	21.84755799424132
3	20.67365676101483	5.301461400554137	51.453686097680226	22.571195740750802
4	19.97718259357853	4.604117998587494	49.14543380235779	26.273265605476176
5	15.727929592003044	22.57554191340251	40.8844461346227	20.81208235997175
6	16.117998587493886	29.726082468626064	40.07692725593524	14.078991687944804
7	79.48432661487477	1.9473026565980334	12.339870701363612	6.228500027163579
8	79.44195143152062	3.3174335850491663	9.74824794914978	7.492367034280437
9	73.20671483674688	5.482696800130385	12.93942521866681	8.371163144455913
10	36.92725593524202	26.317161949258434	20.428098006193295	16.32748410930624
11	28.10343890911066	24.54153311240289	27.156190579670774	20.198837398815666
12	24.398761340794266	21.300157548758627	33.281034389091104	21.020046721356007
13	23.120551963926765	24.631064269028087	32.7188569565926	19.529526810452545
14	18.476883794208725	28.13234095724453	31.165752159504535	22.22502308904221
15	16.522844570000544	24.904003911555385	37.30319986961482	21.26995164882925
16	17.50964307057098	25.009181289726733	32.92747324387461	24.553702395827674
17	17.95664692779921	24.350736132992886	30.243059705546806	27.44955723366111
18	19.412180148856415	23.206606182430598	33.24822078557071	24.132992883142283
19	20.775791818329985	25.189547454772644	30.641821046341068	23.39283968055631
20	22.644211441299504	26.753517683489974	30.167436301407076	20.434834573803446
21	21.944912261639594	26.9649589829956	29.551583636659966	21.538545118704842
22	20.044982886945185	25.719128592383335	28.303145542456676	25.93274297821481
23	19.421089802792416	25.325799967403707	28.404411365241483	26.848698864562394
24	21.994024012603898	23.89351877003314	29.271690117889932	24.840767099473027
25	18.34497745422937	26.868039332862498	27.782039441516815	27.00494377139132
26	17.702830444939423	29.490954528168633	27.568207747052753	25.23800727983919
27	18.424077796490465	28.781441842777205	28.11712935296355	24.677351007768785
28	16.5130656815342	25.49812571304395	32.9902754386918	24.99853316673005
29	19.09534416254686	24.699081871027325	30.407779649046557	25.797794317379257
30	20.445700005432716	26.999945672841854	29.120877926875643	23.433476394849787
31	21.49443146629	27.533438365839082	28.084750366708317	22.887379801162602
32	23.385885804313578	28.382028576085183	25.15477807355897	23.077307546042267
33	20.997229314934536	27.77856250339545	25.00027163579073	26.223936545879283
34	19.596675177921444	24.603031455424567	30.310425381648287	25.489867985005706
35	20.513065681534197	25.316238387569946	26.47253762155702	27.698158309338837
36	21.109577877981202	26.20242299125333	27.325474004454826	25.362525126310643
37	19.93632857065247	25.136958765686966	31.527571032759276	23.399141630901287
38	21.58678763513881	26.40951811810724	27.882870647036455	24.120823599717497
39	21.990981691747706	25.2647362416472	29.195414787852446	23.548867278752645
40	22.01923181398381	24.39745748899875	30.091812897267346	23.491497799750093
41	20.16537186939751	24.004998098549464	27.691204433096107	28.138425598956918
42	21.80865974900853	22.057912750584016	27.590155918943882	28.543271581463575
43	21.96512196447004	21.79149236703428	28.199489324713422	28.043896343782254
44	20.02216548052371	22.777204324441787	28.97375998261531	28.226870212419186
45	21.468354430379748	23.11511924811213	26.99581680882273	28.420709512685395
46	21.810180909436628	24.07888303362851	30.190471016461128	23.920465040473733
47	18.043787689465965	24.17862769598522	32.95289835388711	24.824686260661704
48	19.113380779051447	22.048568479382844	31.362199163361765	27.47585157820394
49	21.283424784049547	20.267941543977834	33.30319986961482	25.145433802357797
50	21.85038300646493	19.88156679524094	30.56706687673168	27.70098332156245
51	19.22638126799587	20.362470799152497	27.653175422393655	32.757972510457975
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	111.0
1	251.0
2	391.0
3	728.0
4	1065.0
5	787.0
6	509.0
7	553.0
8	597.0
9	678.0
10	759.0
11	837.0
12	915.0
13	965.0
14	1015.0
15	990.5
16	966.0
17	895.0
18	824.0
19	890.5
20	957.0
21	1072.0
22	1187.0
23	1383.5
24	1580.0
25	2017.5
26	2992.0
27	3529.0
28	4039.5
29	4550.0
30	5360.0
31	6170.0
32	7027.0
33	7884.0
34	9139.0
35	10394.0
36	11037.5
37	11681.0
38	13620.5
39	15560.0
40	19063.0
41	22566.0
42	26983.5
43	31401.0
44	35894.0
45	40387.0
46	48563.0
47	56739.0
48	61468.0
49	66197.0
50	60482.5
51	54768.0
52	44228.5
53	33689.0
54	27628.5
55	21568.0
56	18564.5
57	15561.0
58	13753.0
59	11945.0
60	11454.0
61	10963.0
62	9303.5
63	7644.0
64	6291.0
65	4938.0
66	4112.5
67	3287.0
68	2788.0
69	2289.0
70	1758.0
71	1227.0
72	1091.5
73	956.0
74	766.5
75	400.0
76	223.0
77	152.0
78	81.0
79	62.5
80	44.0
81	30.0
82	16.0
83	12.0
84	8.0
85	4.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	460175.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.08744539329393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.01610152338303	29.589576387799127
2	11.788833249486638	9.687460847778603
3	4.537611835873761	5.593166355672823
4	2.491436487037772	4.094670424480982
5	1.6401080319723051	3.3693924601382443
6	1.2014143943645332	2.961782899390204
7	0.8938304480851841	2.5707646808594413
8	0.7033330099469559	2.311852531159729
9	0.5727539249092848	2.117969605215451
>10	4.011319527516041	28.414229300036943
>50	0.09514464855360744	2.6471845498675517
>100	0.041085189148147544	3.178043005496235
>500	0.0048653513464911565	1.5401558708492378
>1k	0.0021623783762182917	1.923751081255444
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	2814	0.6115064920953985	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT	2338	0.5080675829847341	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	1936	0.4207095126853915	Illumina Single End Adapter 1 (95% over 21bp)
GCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	1573	0.3418264790568805	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	981	0.21317976856630633	Illumina Single End Adapter 1 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGC	954	0.20731243548649972	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCG	923	0.2005758678763514	No Hit
CTGTCACTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGCT	834	0.18123539957624815	Illumina Single End Adapter 1 (95% over 22bp)
TCTGTATCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	800	0.17384690606834355	Illumina Single End Adapter 1 (95% over 21bp)
TCCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTG	672	0.1460314010974086	No Hit
GCTGTATCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTGC	650	0.14125061118052915	Illumina Single End Adapter 1 (95% over 21bp)
GAATCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTC	604	0.1312544140815994	No Hit
CGCTGTCTCTTATACACATCTGACGCACTGATGCTCGTATGCCGTCTTCTG	516	0.11213125441408159	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5376215570163525	0.0
2	0.0	0.0	0.0	2.041179985874939	0.0
3	0.0	0.0	0.0	2.8030640517194545	0.0
4	0.0	0.0	0.0	3.6055848318574455	0.0
5	0.0	0.0	0.0	4.995490845873852	0.0
6	0.0	0.0	0.0	5.943608409844081	0.0
7	0.0	0.0	0.0	7.170967566686587	0.0
8	0.0	0.0	0.0	9.10544901396208	0.0
9	0.0	0.0	0.0	9.968598902591406	0.0
10	0.0	0.0	0.0	11.687944803607323	0.0
11	0.0	0.0	0.0	14.582061172380072	0.0
12	0.0	0.0	0.0	17.303417178247408	0.0
13	0.0	0.0	0.0	18.19373064594991	0.0
14	0.0	0.0	0.0	18.47514532514804	0.0
15	0.0	0.0	0.0	18.977128266420383	0.0
16	0.0	0.0	0.0	20.053023306350845	0.0
17	0.0	0.0	0.0	21.581572227956755	0.0
18	0.0	0.0	0.0	23.34850871950888	0.0
19	0.0	0.0	0.0	24.474167436301407	0.0
20	0.0	0.0	0.0	25.627858966697453	0.0
21	0.0	0.0	0.0	27.054924756885967	0.0
22	0.0	0.0	0.0	28.512848372901612	0.0
23	0.0	0.0	0.0	29.856033030912155	0.0
24	0.0	0.0	0.0	30.870864345086108	0.0
25	0.0	0.0	0.0	31.74835660346607	0.0
26	0.0	0.0	0.0	32.501982941272345	0.0
27	0.0	0.0	0.0	33.217362959743575	0.0
28	0.0	0.0	0.0	34.02097028304449	0.0
29	0.0	0.0	0.0	34.840658445156734	0.0
30	0.0	0.0	0.0	35.75770087466724	0.0
31	0.0	0.0	0.0	36.60237952952681	0.0
32	0.0	0.0	0.0	37.387081001792794	0.0
33	0.0	0.0	0.0	38.12267072309447	0.0
34	0.0	0.0	0.0	38.93453577443364	0.0
35	0.0	0.0	0.0	39.70598142011191	0.0
36	0.0	0.0	0.0	40.46134622697887	0.0
37	0.0	0.0	0.0	41.16955506057478	0.0
38	0.0	0.0	0.0	41.89884283153148	0.0
39	0.0	0.0	0.0	42.68071929157386	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTGGCGT	45	3.8380676E-10	45.000004	34
TATCAGC	20	7.028873E-4	45.0	45
TAATACG	35	1.2098099E-7	45.0	4
GATACCG	20	7.028873E-4	45.0	21
TCGTTAA	25	3.887147E-5	45.0	34
GACCGTA	20	7.028873E-4	45.0	42
CTATAAG	25	3.887147E-5	45.0	11
TTACTCG	20	7.028873E-4	45.0	45
GGATAGT	35	1.2098099E-7	45.0	8
TAACGGT	20	7.028873E-4	45.0	40
AGGCGAT	55	1.8189894E-12	45.0	6
GCGTTCA	35	1.2098099E-7	45.0	37
ACGGGTC	25	3.887147E-5	45.0	5
AACGGTT	20	7.028873E-4	45.0	10
CGTTAAG	25	3.887147E-5	45.0	35
CCCGTAA	25	3.887147E-5	45.0	16
CCGTACA	20	7.028873E-4	45.0	44
CCGTAAA	25	3.887147E-5	45.0	17
CTACGAA	25	3.887147E-5	45.0	11
TACGAAT	25	3.887147E-5	45.0	12
>>END_MODULE