SRR2934116.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934116.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1007232
Sequences flagged as poor quality	0
Sequence length	51
%GC	49

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	6740	0.6691606303215148	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	5368	0.5329457364341085	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	4366	0.4334651798195451	RNA PCR Primer, Index 38 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	4086	0.4056662218833397	RNA PCR Primer, Index 38 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	3393	0.3368638009912314	RNA PCR Primer, Index 38 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	3177	0.3154188905833016	RNA PCR Primer, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	2640	0.2621044605413648	RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGT	2094	0.2078964925657644	No Hit
GAATCAGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	1625	0.16133323802262042	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTC	1503	0.1492208349218452	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	1474	0.14634165713559538	No Hit
CCTGTATCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1357	0.1347256639979667	RNA PCR Primer, Index 38 (96% over 25bp)
CTGTCACTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	1263	0.12539315669081205	RNA PCR Primer, Index 38 (96% over 26bp)
GCTGTATCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1260	0.12509531071292415	RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTA	1138	0.11298290761214895	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	1102	0.10940875587749396	RNA PCR Primer, Index 27 (95% over 21bp)
TCTGTATCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1087	0.1079195259880544	RNA PCR Primer, Index 27 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACGTT	30	2.1652322E-6	45.000004	27
CGACGTT	25	3.8904614E-5	45.0	27
ACGTTTC	20	7.032871E-4	45.0	17
CGACGGT	20	7.032871E-4	45.0	28
CGACATA	20	7.032871E-4	45.0	14
CACGACG	20	7.032871E-4	45.0	26
TGACCGA	20	7.032871E-4	45.0	11
ACTACGC	20	7.032871E-4	45.0	22
TATTACG	20	7.032871E-4	45.0	1
AATTCGC	25	3.8904614E-5	45.0	13
CGGTCTA	20	7.032871E-4	45.0	31
CGATAGG	20	7.032871E-4	45.0	2
CGATACC	35	1.2117198E-7	45.0	16
TCGGTCG	20	7.032871E-4	45.0	43
GTACGTC	20	7.032871E-4	45.0	22
TACGTCG	20	7.032871E-4	45.0	23
TCGTTAG	150	0.0	43.5	1
CTTTACG	105	0.0	42.857143	1
TACTACG	100	0.0	42.75	4
TACGGGA	535	0.0	42.476635	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content