##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934116.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1007232
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.838509896429024	31.0	31.0	34.0	30.0	34.0
2	32.00712546861101	33.0	31.0	34.0	30.0	34.0
3	32.090464758863895	33.0	31.0	34.0	30.0	34.0
4	35.61020301181853	37.0	35.0	37.0	33.0	37.0
5	29.386911853475663	37.0	35.0	37.0	0.0	37.0
6	23.55617077296988	35.0	10.0	37.0	0.0	37.0
7	30.297912496822978	35.0	19.0	37.0	17.0	37.0
8	34.04604897382132	35.0	32.0	37.0	32.0	37.0
9	36.75046265408565	37.0	35.0	39.0	34.0	39.0
10	36.89251532913966	39.0	37.0	39.0	33.0	39.0
11	37.08125833968738	39.0	37.0	39.0	34.0	39.0
12	36.89933103793366	39.0	35.0	39.0	33.0	39.0
13	36.70060323738721	39.0	35.0	39.0	33.0	39.0
14	37.36640416507815	40.0	36.0	41.0	32.0	41.0
15	37.321882148303466	40.0	36.0	41.0	31.0	41.0
16	37.73531917174991	40.0	36.0	41.0	33.0	41.0
17	37.79447039013852	40.0	36.0	41.0	33.0	41.0
18	37.9102222725251	40.0	36.0	41.0	33.0	41.0
19	37.91939295018427	40.0	36.0	41.0	33.0	41.0
20	37.789955045113736	40.0	36.0	41.0	33.0	41.0
21	37.70534494535519	40.0	36.0	41.0	33.0	41.0
22	37.873686499237515	40.0	36.0	41.0	33.0	41.0
23	37.836441852522555	39.0	36.0	41.0	34.0	41.0
24	37.813591109098994	39.0	36.0	41.0	34.0	41.0
25	37.58590672258229	39.0	35.0	41.0	33.0	41.0
26	37.47627954632101	39.0	35.0	41.0	33.0	41.0
27	36.57686213305376	39.0	35.0	41.0	32.0	41.0
28	36.981858201486844	39.0	35.0	41.0	32.0	41.0
29	37.259948055661454	39.0	35.0	41.0	32.0	41.0
30	37.229769308361924	39.0	35.0	41.0	32.0	41.0
31	37.24310982971153	39.0	35.0	41.0	32.0	41.0
32	37.25626171527513	39.0	35.0	41.0	32.0	41.0
33	36.07737542095565	39.0	35.0	40.0	30.0	41.0
34	36.4820527941924	39.0	35.0	41.0	30.0	41.0
35	36.787094730906084	39.0	35.0	41.0	30.0	41.0
36	36.89578865643665	39.0	35.0	40.0	31.0	41.0
37	36.93659951232685	39.0	35.0	40.0	32.0	41.0
38	36.97471188365739	39.0	35.0	40.0	32.0	41.0
39	37.05220942146397	39.0	35.0	40.0	32.0	41.0
40	36.93527111926547	39.0	35.0	40.0	32.0	41.0
41	36.77173183536663	39.0	35.0	40.0	31.0	41.0
42	36.88462241072563	39.0	35.0	40.0	32.0	41.0
43	36.78175633816241	39.0	35.0	40.0	32.0	41.0
44	36.80813159232431	39.0	35.0	40.0	32.0	41.0
45	36.768899320116915	39.0	35.0	40.0	32.0	41.0
46	36.716083285677975	39.0	35.0	40.0	32.0	41.0
47	36.614497950819676	38.0	35.0	40.0	32.0	41.0
48	36.58867966863642	38.0	35.0	40.0	32.0	41.0
49	36.641683346041425	38.0	35.0	40.0	32.0	41.0
50	36.52780392203584	38.0	35.0	40.0	32.0	41.0
51	35.72828305693226	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	5.0
10	5.0
11	8.0
12	16.0
13	20.0
14	9.0
15	31.0
16	42.0
17	91.0
18	168.0
19	345.0
20	641.0
21	1138.0
22	1755.0
23	2506.0
24	3636.0
25	4903.0
26	6133.0
27	7920.0
28	10068.0
29	13617.0
30	18905.0
31	27127.0
32	39915.0
33	64427.0
34	83359.0
35	90446.0
36	117042.0
37	171997.0
38	189564.0
39	151319.0
40	70.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.32796416317194	4.35748665650019	51.730385849536155	16.584163330791714
2	20.034411138645318	7.935907516838226	49.62084207014868	22.40883927436777
3	21.35208174482145	8.188480906087177	48.997748284407166	21.461689064684204
4	20.326399478968103	4.76613133816241	50.71284470707841	24.19462447579108
5	17.381199167619773	22.428199262930487	39.12355842546702	21.067043143982715
6	16.75810538187826	31.555788537298255	37.25894332189605	14.427162758927437
7	79.61353491549117	2.2855707523192272	13.039597629940273	5.061296702249333
8	79.6051952281103	4.965191733384166	8.80919192400559	6.620421114499936
9	73.94681662218834	5.284681185665269	11.363519030372347	9.40498316177405
10	40.7449326470962	25.819374285169655	18.493653895031134	14.942039172703012
11	34.129872760198246	21.215767568941416	25.16451026178676	19.489849409073578
12	29.95764630194434	18.78008244376668	30.152338289490405	21.109932964798578
13	28.411329266742914	20.345759467530815	32.9554660693862	18.28744519634007
14	21.413338734273733	23.095473535392046	34.662520650654464	20.828667079679754
15	17.69046257466006	20.903128574151737	39.52416126572626	21.88224758546194
16	23.81913997966705	22.684446085906725	29.277465370440968	24.21894856398526
17	24.63881211081459	22.553790983606557	28.661619170161394	24.14577773541746
18	25.669160630321514	20.686793112212477	30.200887183886138	23.44315907357987
19	25.070887342737326	23.861731954505018	28.11497251874444	22.95240818401322
20	24.000031770237644	28.269753145253528	29.184636707332572	18.545578377176263
21	22.898795907993392	26.195255909264205	31.678798926165967	19.22714925657644
22	20.551273192273477	21.552829933917906	33.61410280848901	24.28179406531961
23	20.485151385182363	24.69689207650273	31.314930423179565	23.50302611513534
24	22.854119011310203	25.214349822086668	27.70890916889058	24.222621997712544
25	23.566963718388614	26.144224965052736	24.919482303977635	25.369329012581016
26	21.682889344262296	23.441173433727283	27.253204822722072	27.622732399288346
27	21.390206029991106	26.050899891981196	29.31161837590545	23.247275702122252
28	19.29734162536536	24.13604810013979	29.033231668572878	27.53337860592197
29	22.996985798703776	24.329647985766933	26.930637628669462	25.74272858685983
30	24.96574771254289	24.717741294954887	29.317178167492692	20.99933282500953
31	25.747791968483924	24.825263692972424	26.79809616850934	22.628848170034313
32	26.691765154403356	24.533871044605412	25.94576026178676	22.828603539204472
33	26.14521778497903	25.006056201550386	25.268954918032787	23.579771095437792
34	23.077304930740883	23.567559410344387	29.695343277417713	23.659792381497013
35	21.71922655356462	25.663600838734276	28.05828250095311	24.558890106748
36	28.709473090608718	25.520833333333332	25.18436666031262	20.58532691574533
37	22.374289140932774	28.197575136612024	27.177452662345914	22.25068306010929
38	24.526524177150847	24.279411297496505	26.758184807472357	24.43587971788029
39	22.5962836764519	22.25634213368916	28.141679374761726	27.005694815097215
40	23.520896873808617	21.845215402211206	31.47844786504003	23.155439858940145
41	22.25346295590291	24.48581856017283	26.76940367263947	26.491314811284788
42	22.99102887914602	21.26739420510865	31.537222804676578	24.204354111068753
43	25.10881306392172	20.430645571228872	29.108288855000637	25.35225250984877
44	23.657906023637057	20.997545749142205	28.456800419367134	26.8877478078536
45	22.70013264074215	20.06677706824247	26.53797734782056	30.695112943194815
46	23.73792730969628	21.472610083873427	29.429565383149065	25.35989722328123
47	18.638903450247806	23.595457650273225	34.556288918541114	23.20934998093786
48	21.155006989452282	25.334977443131272	29.08604968865167	24.423965878764772
49	24.078464544414793	20.327392298894395	32.32383403227856	23.27030912441225
50	22.54197642648367	21.28655562968611	28.71999698182742	27.451470962002794
51	20.39827964163172	21.741664283898842	26.720060522302706	31.13999555216673
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	179.0
1	554.5
2	930.0
3	1766.0
4	2602.0
5	1873.0
6	1144.0
7	1229.0
8	1314.0
9	1343.5
10	1373.0
11	1408.5
12	1444.0
13	1401.5
14	1359.0
15	1320.0
16	1281.0
17	1348.0
18	1415.0
19	1522.0
20	1629.0
21	1873.5
22	2118.0
23	2605.0
24	3092.0
25	3763.5
26	5305.0
27	6175.0
28	7376.5
29	8578.0
30	9593.0
31	10608.0
32	12577.0
33	14546.0
34	17410.5
35	20275.0
36	21445.5
37	22616.0
38	25845.0
39	29074.0
40	33362.0
41	37650.0
42	43353.0
43	49056.0
44	58894.0
45	68732.0
46	92978.0
47	117224.0
48	121922.0
49	126620.0
50	121001.5
51	115383.0
52	100169.0
53	84955.0
54	76664.5
55	68374.0
56	61964.0
57	55554.0
58	49829.0
59	44104.0
60	37487.0
61	30870.0
62	26510.0
63	22150.0
64	19682.5
65	17215.0
66	14373.5
67	11532.0
68	9851.5
69	8171.0
70	6696.5
71	5222.0
72	4447.0
73	3672.0
74	3003.5
75	1794.0
76	1253.0
77	884.0
78	515.0
79	432.5
80	350.0
81	215.5
82	81.0
83	44.5
84	8.0
85	9.0
86	10.0
87	8.5
88	7.0
89	4.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1007232.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.46801562222426
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.54535980746736	27.5220801877131
2	11.5784300711526	8.907984577158587
3	4.628777345918855	5.341796377638127
4	2.4820357400151445	3.8191595848728612
5	1.6220368292219316	3.1198269043166182
6	1.216415330059779	2.8075850351911598
7	0.9273310010594421	2.497080840500923
8	0.7720053688954314	2.3758011672888366
9	0.6757268411637637	2.3394483614019514
>10	4.393184775432809	29.48727298026413
>50	0.09596325548325262	2.524536003996832
>100	0.05396155817715001	3.6973675715625447
>500	0.004253127734521747	1.0942400122672133
>1k	0.003987307251114138	3.227706381386281
>5k	5.316409668152184E-4	1.2381140144408391
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGC	6740	0.6691606303215148	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCG	5368	0.5329457364341085	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	4366	0.4334651798195451	RNA PCR Primer, Index 38 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	4086	0.4056662218833397	RNA PCR Primer, Index 38 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	3393	0.3368638009912314	RNA PCR Primer, Index 38 (96% over 26bp)
GCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	3177	0.3154188905833016	RNA PCR Primer, Index 27 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	2640	0.2621044605413648	RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGCTATGT	2094	0.2078964925657644	No Hit
GAATCAGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTC	1625	0.16133323802262042	RNA PCR Primer, Index 38 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTC	1503	0.1492208349218452	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCT	1474	0.14634165713559538	No Hit
CCTGTATCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1357	0.1347256639979667	RNA PCR Primer, Index 38 (96% over 25bp)
CTGTCACTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGCT	1263	0.12539315669081205	RNA PCR Primer, Index 38 (96% over 26bp)
GCTGTATCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1260	0.12509531071292415	RNA PCR Primer, Index 27 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTA	1138	0.11298290761214895	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCT	1102	0.10940875587749396	RNA PCR Primer, Index 27 (95% over 21bp)
TCTGTATCTTATACACATCTGACGCGAGCTATGTCGTATGCCGTCTTCTGC	1087	0.1079195259880544	RNA PCR Primer, Index 27 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3575144554581268	0.0
2	0.0	0.0	0.0	1.4271786440462575	0.0
3	0.0	0.0	0.0	1.935998856271445	0.0
4	0.0	0.0	0.0	2.5853030880670986	0.0
5	0.0	0.0	0.0	3.905356462066336	0.0
6	0.0	0.0	0.0	4.5976497966704795	0.0
7	9.928199262930487E-5	0.0	0.0	5.683397668064557	0.0
8	9.928199262930487E-5	0.0	0.0	7.253840227474901	0.0
9	9.928199262930487E-5	0.0	0.0	7.879515345024781	0.0
10	9.928199262930487E-5	0.0	0.0	9.705609035455586	0.0
11	9.928199262930487E-5	0.0	0.0	12.051046829330284	0.0
12	9.928199262930487E-5	0.0	0.0	14.948293938238658	0.0
13	9.928199262930487E-5	0.0	0.0	15.685661297496505	0.0
14	9.928199262930487E-5	0.0	0.0	15.981918763502351	0.0
15	9.928199262930487E-5	0.0	0.0	16.508709016393443	0.0
16	9.928199262930487E-5	0.0	0.0	17.400261310204602	0.0
17	9.928199262930487E-5	0.0	0.0	18.545578377176263	0.0
18	9.928199262930487E-5	0.0	0.0	19.71363102046003	0.0
19	9.928199262930487E-5	0.0	0.0	20.781607415173465	0.0
20	9.928199262930487E-5	0.0	0.0	21.738586542127337	0.0
21	9.928199262930487E-5	0.0	0.0	23.050995202694118	0.0
22	1.9856398525860974E-4	0.0	0.0	24.38276385182361	0.0
23	1.9856398525860974E-4	0.0	0.0	25.515074977760833	0.0
24	1.9856398525860974E-4	0.0	0.0	26.398585430169017	0.0
25	1.9856398525860974E-4	0.0	0.0	27.173183536662854	0.0
26	1.9856398525860974E-4	0.0	0.0	27.87044097089846	0.0
27	1.9856398525860974E-4	0.0	0.0	28.707983860719278	0.0
28	1.9856398525860974E-4	0.0	0.0	29.486652528910916	0.0
29	1.9856398525860974E-4	0.0	0.0	30.387934457999744	0.0
30	1.9856398525860974E-4	0.0	0.0	31.396043811157707	0.0
31	1.9856398525860974E-4	0.0	0.0	32.15843023255814	0.0
32	1.9856398525860974E-4	0.0	0.0	32.8717713495997	0.0
33	1.9856398525860974E-4	0.0	0.0	33.602685379336634	0.0
34	2.978459778879146E-4	0.0	0.0	34.448965084508835	0.0
35	2.978459778879146E-4	0.0	0.0	35.384499301054774	0.0
36	2.978459778879146E-4	0.0	0.0	36.1346740373618	0.0
37	2.978459778879146E-4	0.0	0.0	36.926646492565766	0.0
38	2.978459778879146E-4	0.0	0.0	37.90844611767696	0.0
39	2.978459778879146E-4	0.0	0.0	39.761743074088194	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGACGTT	30	2.1652322E-6	45.000004	27
CGACGTT	25	3.8904614E-5	45.0	27
ACGTTTC	20	7.032871E-4	45.0	17
CGACGGT	20	7.032871E-4	45.0	28
CGACATA	20	7.032871E-4	45.0	14
CACGACG	20	7.032871E-4	45.0	26
TGACCGA	20	7.032871E-4	45.0	11
ACTACGC	20	7.032871E-4	45.0	22
TATTACG	20	7.032871E-4	45.0	1
AATTCGC	25	3.8904614E-5	45.0	13
CGGTCTA	20	7.032871E-4	45.0	31
CGATAGG	20	7.032871E-4	45.0	2
CGATACC	35	1.2117198E-7	45.0	16
TCGGTCG	20	7.032871E-4	45.0	43
GTACGTC	20	7.032871E-4	45.0	22
TACGTCG	20	7.032871E-4	45.0	23
TCGTTAG	150	0.0	43.5	1
CTTTACG	105	0.0	42.857143	1
TACTACG	100	0.0	42.75	4
TACGGGA	535	0.0	42.476635	4
>>END_MODULE