SRR2934115.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934115.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	361532
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	3644	1.0079329077370744	Illumina PCR Primer Index 5 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	2644	0.7313322195545623	Illumina PCR Primer Index 5 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	2160	0.5974574864742264	RNA PCR Primer, Index 33 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1704	0.4713275726630008	RNA PCR Primer, Index 33 (96% over 27bp)
CCTGTATCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1338	0.3700917207882013	Illumina PCR Primer Index 5 (96% over 25bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCG	1058	0.29264352809709787	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	1031	0.2851753095161701	No Hit
CTGTCACTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	966	0.2671962647843068	Illumina PCR Primer Index 5 (96% over 26bp)
TCTGTATCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	798	0.22072734916964476	RNA PCR Primer, Index 33 (96% over 27bp)
GAATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	724	0.20025889824413884	Illumina PCR Primer Index 5 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	641	0.17730104112499032	Illumina PCR Primer Index 5 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	618	0.17093922529679254	Illumina PCR Primer Index 5 (95% over 24bp)
CCTGACTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	595	0.16457740946859475	RNA PCR Primer, Index 33 (96% over 27bp)
GCTGTATCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	589	0.16291780533949968	RNA PCR Primer, Index 33 (96% over 27bp)
TGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	482	0.13332153170397087	RNA PCR Primer, Index 33 (96% over 26bp)
TTCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	468	0.1294491220694157	RNA PCR Primer, Index 33 (96% over 25bp)
CTGTATCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	460	0.12723631656395562	Illumina PCR Primer Index 5 (96% over 26bp)
CGTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	386	0.10676786563844971	RNA PCR Primer, Index 33 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	380	0.10510826150935462	No Hit
GCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	363	0.10040604981025193	RNA PCR Primer, Index 33 (96% over 26bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	110	0.0	45.000004	5
TCGTTTG	35	1.2088458E-7	45.000004	1
GCGAATC	35	1.2088458E-7	45.000004	17
TTCGTAG	35	1.2088458E-7	45.000004	1
CGTTAGG	65	0.0	45.000004	2
GAGCGAA	35	1.2088458E-7	45.000004	15
AATCTCG	35	1.2088458E-7	45.000004	33
CGAATCG	35	1.2088458E-7	45.000004	18
CCTTAAC	40	6.7921064E-9	45.0	34
GCAAGTC	25	3.8854825E-5	45.0	9
CCCTCTA	20	7.026864E-4	45.0	28
GTATTGC	25	3.8854825E-5	45.0	29
AGTCAAC	25	3.8854825E-5	45.0	12
GTTTACG	20	7.026864E-4	45.0	1
GACCGTT	20	7.026864E-4	45.0	44
TTAGACC	25	3.8854825E-5	45.0	19
CGTTGAA	25	3.8854825E-5	45.0	18
GGATCAT	40	6.7921064E-9	45.0	8
GCGTTAG	25	3.8854825E-5	45.0	1
CGTTCAT	25	3.8854825E-5	45.0	17

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content