##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934115.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	361532
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.823647699235476	31.0	31.0	34.0	30.0	34.0
2	32.0361295818904	33.0	31.0	34.0	30.0	34.0
3	32.108159167100006	33.0	31.0	34.0	30.0	34.0
4	35.5819235918259	37.0	35.0	37.0	33.0	37.0
5	30.184749897657746	37.0	35.0	37.0	0.0	37.0
6	24.306188663797396	35.0	17.0	37.0	0.0	37.0
7	30.599573481738823	35.0	19.0	37.0	17.0	37.0
8	34.22398847128331	35.0	32.0	37.0	32.0	37.0
9	36.79318566544593	38.0	37.0	39.0	34.0	39.0
10	36.91324419415155	39.0	37.0	39.0	33.0	39.0
11	37.113248619762565	39.0	37.0	39.0	34.0	39.0
12	37.08951074870274	39.0	37.0	39.0	34.0	39.0
13	36.93865549937488	39.0	37.0	39.0	33.0	39.0
14	37.96436553334145	40.0	37.0	41.0	33.0	41.0
15	37.85991834747685	40.0	37.0	41.0	33.0	41.0
16	38.11499673611188	40.0	37.0	41.0	34.0	41.0
17	38.142020623347314	40.0	37.0	41.0	34.0	41.0
18	38.245477578748215	40.0	37.0	41.0	34.0	41.0
19	38.26815053715853	40.0	37.0	41.0	34.0	41.0
20	38.218453691512785	40.0	37.0	41.0	34.0	41.0
21	38.095571069780824	40.0	37.0	41.0	34.0	41.0
22	38.1449138665457	40.0	37.0	41.0	34.0	41.0
23	38.107047232333514	40.0	37.0	41.0	34.0	41.0
24	38.106944890078886	40.0	37.0	41.0	34.0	41.0
25	37.85954217054092	40.0	36.0	41.0	34.0	41.0
26	37.691866833364685	39.0	36.0	41.0	33.0	41.0
27	36.969728820685305	39.0	36.0	41.0	32.0	41.0
28	37.27145591538232	39.0	36.0	41.0	32.0	41.0
29	37.28585021519534	39.0	35.0	41.0	32.0	41.0
30	37.34859984731642	39.0	36.0	40.0	33.0	41.0
31	37.29011816381399	39.0	35.0	40.0	33.0	41.0
32	37.23451036146178	39.0	35.0	40.0	32.0	41.0
33	36.33855924233539	39.0	35.0	40.0	31.0	41.0
34	36.76550900058639	39.0	35.0	40.0	31.0	41.0
35	37.01431408561344	39.0	35.0	40.0	31.0	41.0
36	37.17434417976832	39.0	35.0	40.0	32.0	41.0
37	37.18452031908655	39.0	35.0	40.0	33.0	41.0
38	37.052645408981775	39.0	35.0	40.0	32.0	41.0
39	37.068749101047764	39.0	35.0	40.0	32.0	41.0
40	37.09231824568779	39.0	35.0	40.0	32.0	41.0
41	37.10977728112587	39.0	35.0	40.0	32.0	41.0
42	37.28456955400905	39.0	35.0	40.0	33.0	41.0
43	37.19014914309107	39.0	35.0	40.0	33.0	41.0
44	37.12983636303287	39.0	35.0	40.0	32.0	41.0
45	37.02660345418939	39.0	35.0	40.0	32.0	41.0
46	36.98369438943164	39.0	35.0	40.0	32.0	41.0
47	36.93814102209486	39.0	35.0	40.0	32.0	41.0
48	36.94927419979421	39.0	35.0	40.0	32.0	41.0
49	37.04965535554253	39.0	35.0	40.0	32.0	41.0
50	36.98119115320359	39.0	35.0	40.0	32.0	41.0
51	36.21899306285474	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	1.0
11	2.0
12	1.0
13	3.0
14	4.0
15	14.0
16	17.0
17	33.0
18	29.0
19	74.0
20	135.0
21	240.0
22	349.0
23	556.0
24	827.0
25	1188.0
26	1693.0
27	2211.0
28	2861.0
29	3872.0
30	5702.0
31	8239.0
32	12166.0
33	19827.0
34	27063.0
35	35509.0
36	45199.0
37	66372.0
38	74936.0
39	52377.0
40	29.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.074582609561535	3.940730004536251	54.18662801633051	18.798059369571714
2	20.119934058395938	5.66146288571966	52.56491818151644	21.65368487436797
3	21.96873305820785	5.466459400550989	51.623646039631346	20.941161501609816
4	20.671475830631866	4.532378876558645	50.57671243486054	24.21943285794895
5	15.768175431220474	20.055486098049414	42.100007744819266	22.076330725910847
6	15.599725612117323	29.163670159211357	39.826903289335384	15.409700939335938
7	76.73705232178618	2.1392297224035492	13.909418806633992	7.2142991491762825
8	75.86188774437672	3.1792483099697955	11.312691545976566	9.64617239967693
9	69.6098823893874	5.938340174590354	15.127291636701592	9.324485799320668
10	34.778664129316354	26.11774338094553	21.550789418364072	17.552803071374043
11	27.506278835621746	24.41415974242944	27.31984997178673	20.759711450162087
12	23.809234037374285	20.071252337275816	34.146631556819315	21.972882068530588
13	21.87966763661308	25.73631103194185	33.19014637708418	19.193874954360886
14	17.2972240354934	28.670767732870118	31.198344821481918	22.833663410154564
15	14.850137747142714	25.113129681466646	38.55149751612582	21.485235055264816
16	15.882688116128033	24.336711549738336	32.943418563225386	26.83718177090825
17	16.073542590973965	24.82269895887501	29.650210769724396	29.453547680426627
18	17.641038690904264	23.028666895323234	34.25976123828596	25.070533175486542
19	19.19968356881272	24.327583727028312	31.68875784162951	24.78397486252946
20	21.227719814566896	25.37673013730458	32.10061626633327	21.29493378179525
21	21.207804565017756	26.737328922474358	31.052852859497914	21.002013653009968
22	18.20530409479659	25.4160074350265	28.2566965026609	28.121991967516013
23	18.211942511312966	26.38936525674076	27.486640186760784	27.912052045185487
24	20.845457663498667	23.179414270382708	29.638316940132547	26.33681112598608
25	17.270117168051513	27.333403405507674	27.482214575749865	27.91426485069095
26	18.367115497383356	29.001582155936408	27.827135633913457	24.804166712766783
27	21.977031078853322	28.839494152661455	26.947268844804885	22.236205923680338
28	19.10176692519611	24.564907117488907	32.134638150979725	24.19868780633526
29	19.74348052177954	24.481097108969607	31.620437471648426	24.154984897602425
30	26.431961762720867	25.698416737660846	26.89499131473839	20.9746301848799
31	24.659504552847327	29.498080391224015	24.058174656738547	21.784240399190114
32	21.648706061980683	27.789517940320636	27.72894238960866	22.832833608090016
33	23.33514045782946	26.62336943894316	24.299647057521877	25.741843045705497
34	20.508281424604185	25.598563889226956	28.679895555580142	25.213259130588717
35	20.057698903554872	24.397287100450306	28.765918369604904	26.77909562638992
36	26.86069282940376	24.200624011152538	25.987464456811573	22.951218702632133
37	23.40014161955235	25.03844749565737	30.81774227454278	20.7436686102475
38	22.048670657092593	25.3131119790226	29.58603941006605	23.05217795381875
39	23.179414270382708	22.80102452894903	30.48443844528285	23.535122755385416
40	24.379308055718443	23.688359536638526	29.47014372171758	22.46218868592545
41	18.738866822300654	25.668267262648946	26.434727769602688	29.158138145447705
42	21.823517696912027	22.384463892546165	28.160716063861567	27.631302346680236
43	25.57781883761327	20.31576734562916	27.940818516756472	26.165595300001105
44	22.911941404910216	21.08361085602381	28.997156544925485	27.007291194140493
45	23.234457807331026	20.986800615159932	27.37268070322959	28.406060874279454
46	25.919420687518667	22.061947490125355	30.181284091034822	21.837347731321156
47	19.641691468528375	23.107221490767067	33.727304913534624	23.52378212716993
48	19.437836761337863	21.592832722967813	32.224256773950856	26.745073741743468
49	22.738789374107963	18.015556022703386	34.37869953420444	24.86695506898421
50	23.061305776528773	18.325901994844163	31.10734319506987	27.505449033557195
51	20.38878992730934	18.270305256519478	28.24590907582178	33.094995740349404
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	97.0
1	196.0
2	295.0
3	588.0
4	881.0
5	624.5
6	368.0
7	473.0
8	578.0
9	699.5
10	821.0
11	892.5
12	964.0
13	1027.5
14	1091.0
15	1007.5
16	924.0
17	869.5
18	815.0
19	821.0
20	827.0
21	880.5
22	934.0
23	1013.0
24	1092.0
25	1206.0
26	1665.0
27	2010.0
28	2574.0
29	3138.0
30	3388.0
31	3638.0
32	4423.0
33	5208.0
34	5785.5
35	6363.0
36	7225.0
37	8087.0
38	8982.0
39	9877.0
40	12352.0
41	14827.0
42	18660.0
43	22493.0
44	26980.5
45	31468.0
46	40565.5
47	49663.0
48	54267.5
49	58872.0
50	53204.0
51	47536.0
52	37250.0
53	26964.0
54	22162.0
55	17360.0
56	14514.0
57	11668.0
58	10402.0
59	9136.0
60	7946.5
61	6757.0
62	6121.0
63	5485.0
64	4479.0
65	3473.0
66	2845.0
67	2217.0
68	1817.0
69	1417.0
70	1224.0
71	1031.0
72	876.0
73	721.0
74	615.0
75	391.0
76	273.0
77	229.0
78	185.0
79	121.5
80	58.0
81	55.5
82	53.0
83	39.5
84	26.0
85	18.5
86	11.0
87	6.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	361532.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.913874873271126
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.96481204189139	30.88288939244202
2	12.30756074305826	10.563302434455721
3	4.844720445878741	6.237171810312557
4	2.6328980614161104	4.519514318467563
5	1.6659203402728264	3.5745548515652663
6	1.1571971787442628	2.9795888959400187
7	0.8565708221551943	2.5731141157444086
8	0.7016403439303643	2.4088084740373255
9	0.5331448687641617	2.0591380968724606
>10	3.194715556637014	22.497567904764683
>50	0.0833017335854828	2.5162621209379488
>100	0.04826211549000194	3.936535903485628
>500	0.004627874088082378	1.398995210096406
>1k	0.004627874088082378	3.8525564708779347
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	3644	1.0079329077370744	Illumina PCR Primer Index 5 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	2644	0.7313322195545623	Illumina PCR Primer Index 5 (96% over 26bp)
TCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	2160	0.5974574864742264	RNA PCR Primer, Index 33 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1704	0.4713275726630008	RNA PCR Primer, Index 33 (96% over 27bp)
CCTGTATCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	1338	0.3700917207882013	Illumina PCR Primer Index 5 (96% over 25bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCG	1058	0.29264352809709787	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGC	1031	0.2851753095161701	No Hit
CTGTCACTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	966	0.2671962647843068	Illumina PCR Primer Index 5 (96% over 26bp)
TCTGTATCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	798	0.22072734916964476	RNA PCR Primer, Index 33 (96% over 27bp)
GAATCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	724	0.20025889824413884	Illumina PCR Primer Index 5 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	641	0.17730104112499032	Illumina PCR Primer Index 5 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	618	0.17093922529679254	Illumina PCR Primer Index 5 (95% over 24bp)
CCTGACTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	595	0.16457740946859475	RNA PCR Primer, Index 33 (96% over 27bp)
GCTGTATCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGC	589	0.16291780533949968	RNA PCR Primer, Index 33 (96% over 27bp)
TGCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	482	0.13332153170397087	RNA PCR Primer, Index 33 (96% over 26bp)
TTCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCT	468	0.1294491220694157	RNA PCR Primer, Index 33 (96% over 25bp)
CTGTATCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTGCT	460	0.12723631656395562	Illumina PCR Primer Index 5 (96% over 26bp)
CGTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTC	386	0.10676786563844971	RNA PCR Primer, Index 33 (95% over 24bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCC	380	0.10510826150935462	No Hit
GCCTGTCTCTTATACACATCTGACGCAGGTGATCTCGTATGCCGTCTTCTG	363	0.10040604981025193	RNA PCR Primer, Index 33 (96% over 26bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7877587599437947	0.0
2	0.0	0.0	0.0	3.0697144374495204	0.0
3	0.0	0.0	0.0	4.106690417445758	0.0
4	0.0	0.0	0.0	5.230519013531306	0.0
5	0.0	0.0	0.0	7.134084949603355	0.0
6	0.0	0.0	0.0	8.395937289091975	0.0
7	0.0	0.0	0.0	10.051669008552492	0.0
8	0.0	0.0	0.0	12.523372758151423	0.0
9	0.0	0.0	0.0	13.564774349158581	0.0
10	0.0	0.0	0.0	15.774260646360489	0.0
11	0.0	0.0	0.0	19.25721651195468	0.0
12	0.0	0.0	0.0	22.67157540687961	0.0
13	0.0	0.0	0.0	23.7251474281668	0.0
14	0.0	0.0	0.0	24.08721772899771	0.0
15	0.0	0.0	0.0	24.64207870949183	0.0
16	0.0	0.0	0.0	26.01429472356527	0.0
17	0.0	0.0	0.0	27.999457862651163	0.0
18	0.0	0.0	0.0	30.376840777579854	0.0
19	0.0	0.0	0.0	31.776440259783367	0.0
20	0.0	0.0	0.0	33.122655809167654	0.0
21	0.0	0.0	0.0	34.7637276921545	0.0
22	0.0	0.0	0.0	36.30328712257836	0.0
23	0.0	0.0	0.0	37.69901419514732	0.0
24	0.0	0.0	0.0	38.83307701669562	0.0
25	0.0	0.0	0.0	39.76356173174159	0.0
26	0.0	0.0	0.0	40.587555181837295	0.0
27	0.0	0.0	0.0	41.377250146598364	0.0
28	0.0	0.0	0.0	42.208435214586814	0.0
29	0.0	0.0	0.0	43.078620979609	0.0
30	0.0	0.0	0.0	44.04561698549506	0.0
31	0.0	0.0	0.0	44.89699390372083	0.0
32	0.0	0.0	0.0	45.73868979786022	0.0
33	0.0	0.0	0.0	46.53861898808404	0.0
34	0.0	0.0	0.0	47.31780312669418	0.0
35	0.0	0.0	0.0	48.14705198986535	0.0
36	0.0	0.0	0.0	48.890831240388124	0.0
37	0.0	0.0	0.0	49.684951816160115	0.0
38	0.0	0.0	0.0	50.3875175641437	0.0
39	0.0	0.0	0.0	51.12991381122556	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCG	110	0.0	45.000004	5
TCGTTTG	35	1.2088458E-7	45.000004	1
GCGAATC	35	1.2088458E-7	45.000004	17
TTCGTAG	35	1.2088458E-7	45.000004	1
CGTTAGG	65	0.0	45.000004	2
GAGCGAA	35	1.2088458E-7	45.000004	15
AATCTCG	35	1.2088458E-7	45.000004	33
CGAATCG	35	1.2088458E-7	45.000004	18
CCTTAAC	40	6.7921064E-9	45.0	34
GCAAGTC	25	3.8854825E-5	45.0	9
CCCTCTA	20	7.026864E-4	45.0	28
GTATTGC	25	3.8854825E-5	45.0	29
AGTCAAC	25	3.8854825E-5	45.0	12
GTTTACG	20	7.026864E-4	45.0	1
GACCGTT	20	7.026864E-4	45.0	44
TTAGACC	25	3.8854825E-5	45.0	19
CGTTGAA	25	3.8854825E-5	45.0	18
GGATCAT	40	6.7921064E-9	45.0	8
GCGTTAG	25	3.8854825E-5	45.0	1
CGTTCAT	25	3.8854825E-5	45.0	17
>>END_MODULE