FastQCFastQC Report
Sat 14 Jan 2017
SRR2934114.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934114.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1179359
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC37000.31372974641309387TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT33160.28116968624481603TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC29930.2537819273011865TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC25450.21579519043819564TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCACTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT12670.10743124019064593TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTATCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC12530.10624415466367748TruSeq Adapter, Index 20 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG12250.10386998360974053TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC12220.10361560813967588TruSeq Adapter, Index 20 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC207.033362E-445.011
CCTTACG253.8908678E-545.01
ACACCGT207.033362E-445.025
TTATCCG253.8908678E-545.01
GTCGTAG502.1827873E-1145.01
CGCAAGA207.033362E-445.035
TCGCGCG406.8157533E-945.01
AACGGGT302.1655505E-644.9999964
TCGTGCG650.044.9999961
TTCGCAG650.044.9999961
CGTTAGG3000.042.752
TAGGGCG7000.042.755
GCGCTAG1000.042.751
TTCGTAG1750.042.428571
CGTTGTC650.041.5384628
TATGGGT12450.041.3855444
CGCGAGG603.6379788E-1241.2499962
TCGTTAG1700.041.029411
CTTACGG2650.040.754722
AGGGCGA9450.040.7142836
Produced by FastQC (version 0.11.5)