##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934114.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1179359
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.967142320531746	33.0	31.0	34.0	30.0	34.0
2	32.17695799158695	33.0	31.0	34.0	30.0	34.0
3	32.249695809333716	34.0	31.0	34.0	30.0	34.0
4	35.700975699511346	37.0	35.0	37.0	35.0	37.0
5	29.215790102928793	37.0	35.0	37.0	0.0	37.0
6	23.400123287311157	35.0	10.0	37.0	0.0	37.0
7	30.31007352299003	35.0	19.0	37.0	17.0	37.0
8	34.34859529625839	35.0	32.0	37.0	32.0	37.0
9	36.97534084193193	38.0	37.0	39.0	34.0	39.0
10	36.84239489417556	39.0	37.0	39.0	33.0	39.0
11	37.05189768340259	39.0	37.0	39.0	34.0	39.0
12	37.198442543788616	39.0	37.0	39.0	34.0	39.0
13	37.225765860946495	39.0	37.0	39.0	34.0	39.0
14	38.39293548444537	40.0	38.0	41.0	34.0	41.0
15	38.23972598674364	40.0	38.0	41.0	34.0	41.0
16	38.4212864785023	40.0	38.0	41.0	34.0	41.0
17	38.42796807418267	40.0	38.0	41.0	34.0	41.0
18	38.485211034129556	40.0	38.0	41.0	34.0	41.0
19	38.499482345918416	40.0	38.0	41.0	34.0	41.0
20	38.46665773526127	40.0	38.0	41.0	34.0	41.0
21	38.357056672310975	40.0	38.0	41.0	34.0	41.0
22	38.4221861197481	40.0	38.0	41.0	34.0	41.0
23	38.365762248814825	40.0	38.0	41.0	34.0	41.0
24	38.35147652241599	40.0	38.0	41.0	34.0	41.0
25	38.13783334845454	40.0	37.0	41.0	34.0	41.0
26	38.03678184505312	40.0	37.0	41.0	34.0	41.0
27	37.166216563404355	40.0	37.0	41.0	33.0	41.0
28	37.55237124573603	40.0	37.0	41.0	33.0	41.0
29	37.86446196620368	40.0	37.0	41.0	33.0	41.0
30	37.90148462003512	40.0	37.0	41.0	33.0	41.0
31	37.95568016185063	40.0	37.0	41.0	34.0	41.0
32	37.972054310858695	40.0	37.0	41.0	34.0	41.0
33	36.7487881128647	40.0	37.0	41.0	32.0	41.0
34	37.29620582028034	40.0	37.0	41.0	32.0	41.0
35	37.6425761790939	40.0	37.0	41.0	32.0	41.0
36	37.800060880529166	40.0	37.0	41.0	33.0	41.0
37	37.874871858356954	40.0	37.0	41.0	34.0	41.0
38	37.80523233383558	40.0	37.0	41.0	33.0	41.0
39	37.855552889323775	40.0	37.0	41.0	33.0	41.0
40	37.87828048965582	40.0	37.0	41.0	33.0	41.0
41	37.83299402472021	40.0	37.0	41.0	33.0	41.0
42	37.886058443612164	40.0	37.0	41.0	33.0	41.0
43	37.77434352050563	40.0	37.0	41.0	33.0	41.0
44	37.7901012329579	40.0	37.0	41.0	33.0	41.0
45	37.741941173128794	40.0	37.0	41.0	33.0	41.0
46	37.708816399416975	40.0	37.0	41.0	33.0	41.0
47	37.68910230048696	40.0	37.0	41.0	33.0	41.0
48	37.641668906584	40.0	36.0	41.0	33.0	41.0
49	37.68326777512191	40.0	36.0	41.0	33.0	41.0
50	37.62298078871658	40.0	36.0	41.0	33.0	41.0
51	36.896095251742686	39.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	4.0
10	10.0
11	9.0
12	11.0
13	14.0
14	19.0
15	20.0
16	31.0
17	58.0
18	108.0
19	180.0
20	340.0
21	573.0
22	1040.0
23	1592.0
24	2587.0
25	3671.0
26	5004.0
27	6157.0
28	7840.0
29	10482.0
30	15063.0
31	21858.0
32	32351.0
33	51021.0
34	73390.0
35	99330.0
36	139880.0
37	219556.0
38	257255.0
39	229805.0
40	96.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.5489787248836	4.540602140654372	58.6854384457998	13.22498068866223
2	19.54120840219136	4.0949363171010695	53.84000970018459	22.52384558052298
3	20.222341119201193	4.00980532645276	53.11342856585654	22.65442498848951
4	18.937914579021317	4.560782594612836	51.76108377516939	24.740219051196455
5	13.833277229410212	23.048283007972977	43.39060455722134	19.727835205395472
6	15.034692574525653	29.892339821886292	42.04453436146245	13.028433242125596
7	83.63331267239238	1.8228546184834302	9.754620942393283	4.789211766730911
8	85.7950802088253	1.7344167467242797	7.130229217736075	5.340273826714342
9	80.38213978949582	4.3017435742636465	9.946928797762174	5.36918783847836
10	36.65906649289995	20.986739406745528	23.515061995541643	18.83913210481287
11	26.931663725803595	27.769491732373265	23.446634994094246	21.852209547728894
12	21.379071173408608	23.219647282973206	32.515290085546475	22.885991458071715
13	22.400558269364968	25.187580711216857	33.25654020531492	19.155320814103256
14	20.958758105038413	26.102738860686188	32.16153859850987	20.776964435765528
15	19.784900102513316	26.50838294361598	32.99487263844173	20.711844315428973
16	19.91522513500978	25.26533481323329	32.08285178643653	22.7365882653204
17	20.436610056819003	26.32955698816052	28.83278119724359	24.401051757776894
18	20.920432200882004	25.260756054772127	31.917422939071137	21.90138880527473
19	22.434898957823698	27.85809918777912	28.85016352103134	20.856838333365836
20	23.169280939900403	27.048422066563276	29.699947174694053	20.082349818842268
21	23.356501285868	27.215207583102348	28.994818371674786	20.43347275935487
22	21.337099220847936	25.96410422950094	28.065499987705184	24.63329656194594
23	20.42431524243254	26.557986160278592	27.899223222106244	25.11847537518262
24	21.287665587832034	25.6495265648543	29.077829566739222	23.98497828057445
25	20.07539688932717	26.820162478091913	27.62780459554724	25.47663603703368
26	19.723256446934307	26.994833634202987	30.55592063146167	22.72598928740104
27	20.71769495124046	27.973585651188486	29.40215829107167	21.906561106499378
28	18.46375870282077	25.80995269464175	32.23844478229275	23.487843820244724
29	19.621591050731794	24.931085445568314	31.20949600588116	24.23782749781873
30	21.222036716555348	25.220225563208487	30.226504397727915	23.331233322508243
31	19.178214606409075	27.369189534314824	29.85621850513711	23.596377354138987
32	18.147485201707028	27.796879491316894	29.20874814199917	24.84688716497691
33	19.80703076840894	27.991815893209786	27.19697734108105	25.004175997300226
34	18.14994416458432	24.90132351557075	30.61408782228312	26.33464449756181
35	17.961536733089755	24.57122894725016	30.027921947430762	27.439312372229317
36	20.92662200398691	24.88699369742377	29.164656393854628	25.021727904734693
37	18.640719238162426	26.667028445112983	31.45725771372415	23.234994603000445
38	17.762021572735698	28.350146138707554	28.623006226263588	25.26482606229316
39	18.588657058622523	25.030122295246827	29.700710301104245	26.68051034502641
40	19.482023709489646	23.132142121270963	30.969111186670045	26.416722982569347
41	18.044887095447613	23.45112896073206	29.634148719770653	28.869835224049673
42	19.797025333253064	23.266452369465107	29.122684441293956	27.81383785598787
43	20.29509250363969	23.69600774658098	29.94753929889033	26.061360450888998
44	18.990061550384574	24.441667041163885	29.336444627971638	27.231826780479906
45	20.203432542593053	23.392113851677053	28.983117100051807	27.421336505678084
46	20.713200984602654	23.030052765951673	30.5348074674463	25.721938781999377
47	18.06812005504685	23.551946438701023	32.67342683610334	25.706506670148784
48	17.977986346820604	23.274168425390403	31.676529368919898	27.0713158588691
49	19.587928696859905	21.74588060124186	32.21648370004384	26.449707001854396
50	19.169057089486746	21.207198147468244	30.770359152726183	28.853385610318828
51	18.137903725667925	20.89482507022883	28.83863183305507	32.12863937104817
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	109.0
1	342.0
2	575.0
3	1629.0
4	2683.0
5	1985.5
6	1288.0
7	1276.5
8	1265.0
9	1386.0
10	1507.0
11	1552.0
12	1597.0
13	1637.0
14	1677.0
15	1709.5
16	1742.0
17	1750.0
18	1758.0
19	2140.5
20	2523.0
21	2723.0
22	2923.0
23	3407.5
24	3892.0
25	4856.0
26	7189.0
27	8558.0
28	10516.0
29	12474.0
30	14938.0
31	17402.0
32	20863.0
33	24324.0
34	28300.0
35	32276.0
36	35885.5
37	39495.0
38	45807.5
39	52120.0
40	59603.0
41	67086.0
42	75808.5
43	84531.0
44	94545.5
45	104560.0
46	122306.5
47	140053.0
48	151223.5
49	162394.0
50	147461.5
51	132529.0
52	110503.0
53	88477.0
54	72789.0
55	57101.0
56	48225.5
57	39350.0
58	33945.0
59	28540.0
60	24739.5
61	20939.0
62	17677.5
63	14416.0
64	11975.0
65	9534.0
66	7420.5
67	5307.0
68	4263.0
69	3219.0
70	2747.5
71	2276.0
72	1824.0
73	1372.0
74	1069.0
75	560.5
76	355.0
77	294.0
78	233.0
79	181.0
80	129.0
81	132.0
82	135.0
83	89.5
84	44.0
85	24.5
86	5.0
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1179359.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.792045415747914
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.75957830159521	33.77601959433062
2	11.475858543332977	10.51006071202005
3	4.505965667299688	6.190121534363645
4	2.3448909627305152	4.295094138413305
5	1.500747720137374	3.436115387905539
6	1.087518001552284	2.987980423051537
7	0.8418789757679193	2.6985952205049535
8	0.674457369131448	2.47078259826025
9	0.537760493098097	2.216263763046875
>10	3.1495242844248303	23.580758479703068
>50	0.08075588104408114	2.51632456443781
>100	0.03707702379231863	2.926479290441902
>500	0.002192726851323021	0.6977543792268266
>1k	0.0017940492419915629	1.6976499142936319
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	3700	0.31372974641309387	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT	3316	0.28116968624481603	TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	2993	0.2537819273011865	TruSeq Adapter, Index 23 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	2545	0.21579519043819564	TruSeq Adapter, Index 23 (95% over 22bp)
CTGTCACTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGCT	1267	0.10743124019064593	TruSeq Adapter, Index 20 (95% over 23bp)
TCTGTATCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	1253	0.10624415466367748	TruSeq Adapter, Index 20 (95% over 22bp)
TCCTGTCTCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTG	1225	0.10386998360974053	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCTGTACTCCTCGTATGCCGTCTTCTGC	1222	0.10361560813967588	TruSeq Adapter, Index 20 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2971105490355354	0.0
2	0.0	0.0	0.0	1.1481660800485687	0.0
3	0.0	0.0	0.0	1.643180744794418	0.0
4	0.0	0.0	0.0	2.1498966811632423	0.0
5	0.0	0.0	0.0	3.032918729581069	0.0
6	0.0	0.0	0.0	3.8268245716529066	0.0
7	0.0	0.0	0.0	4.722734977220677	0.0
8	0.0	0.0	0.0	6.253227388776446	0.0
9	0.0	0.0	0.0	6.890013982171671	0.0
10	0.0	0.0	0.0	8.198775775654402	0.0
11	0.0	0.0	0.0	10.736425465019558	0.0
12	0.0	0.0	0.0	12.721826008874313	0.0
13	0.0	0.0	0.0	13.426530852776805	0.0
14	0.0	0.0	0.0	13.65012689096365	0.0
15	0.0	0.0	0.0	13.98234125486811	0.0
16	0.0	0.0	0.0	14.910302969664029	0.0
17	0.0	0.0	0.0	16.292070523055322	0.0
18	0.0	0.0	0.0	17.815016462332505	0.0
19	0.0	0.0	0.0	18.804367457237365	0.0
20	0.0	0.0	0.0	19.80770910299578	0.0
21	0.0	0.0	0.0	21.37432283130073	0.0
22	0.0	0.0	0.0	22.97968642287887	0.0
23	0.0	0.0	0.0	24.663228075590215	0.0
24	0.0	0.0	0.0	25.88363678913715	0.0
25	0.0	0.0	0.0	26.961340863977806	0.0
26	0.0	0.0	0.0	27.907871988088445	0.0
27	0.0	0.0	0.0	28.8000515534286	0.0
28	0.0	0.0	0.0	29.787028377279523	0.0
29	0.0	0.0	0.0	30.782993134406063	0.0
30	0.0	0.0	0.0	31.82364318244063	0.0
31	0.0	0.0	0.0	32.8625973940081	0.0
32	0.0	0.0	0.0	33.87365509569181	0.0
33	0.0	0.0	0.0	34.86097108683615	0.0
34	0.0	0.0	0.0	35.77468777530845	0.0
35	0.0	0.0	0.0	36.735294342096005	0.0
36	0.0	0.0	0.0	37.71328323267131	0.0
37	0.0	0.0	0.0	38.67304188122531	0.0
38	0.0	0.0	0.0	39.65094597997726	0.0
39	0.0	0.0	0.0	40.591372092806346	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACGC	20	7.033362E-4	45.0	11
CCTTACG	25	3.8908678E-5	45.0	1
ACACCGT	20	7.033362E-4	45.0	25
TTATCCG	25	3.8908678E-5	45.0	1
GTCGTAG	50	2.1827873E-11	45.0	1
CGCAAGA	20	7.033362E-4	45.0	35
TCGCGCG	40	6.8157533E-9	45.0	1
AACGGGT	30	2.1655505E-6	44.999996	4
TCGTGCG	65	0.0	44.999996	1
TTCGCAG	65	0.0	44.999996	1
CGTTAGG	300	0.0	42.75	2
TAGGGCG	700	0.0	42.75	5
GCGCTAG	100	0.0	42.75	1
TTCGTAG	175	0.0	42.42857	1
CGTTGTC	65	0.0	41.53846	28
TATGGGT	1245	0.0	41.385544	4
CGCGAGG	60	3.6379788E-12	41.249996	2
TCGTTAG	170	0.0	41.02941	1
CTTACGG	265	0.0	40.75472	2
AGGGCGA	945	0.0	40.714283	6
>>END_MODULE