SRR2934113.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934113.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	805348
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGC	5083	0.6311557239851593	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCG	4090	0.5078549893958885	No Hit
CCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	3017	0.3746206608820038	TruSeq Adapter, Index 22 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTC	3004	0.373006451869254	No Hit
CTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT	2597	0.3224692927777805	TruSeq Adapter, Index 20 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	2574	0.319613384524454	TruSeq Adapter, Index 22 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	2030	0.2520649458370791	TruSeq Adapter, Index 22 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATTCGTCCT	1602	0.19892021834039447	No Hit
GAATCAGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTC	1158	0.143788772058787	No Hit
GCTGTATCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1092	0.13559355707098047	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTATCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	1064	0.1321167991973656	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTC	1055	0.13099926988084654	No Hit
GAATGACTGTCTCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCT	1039	0.12901255109592374	No Hit
CTGTCACTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGCT	935	0.11609887899392561	TruSeq Adapter, Index 20 (95% over 22bp)
TCTGTATCTTATACACATCTGACGCATTCGTCCTCGTATGCCGTCTTCTGC	857	0.10641362491742701	TruSeq Adapter, Index 20 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGTTC	20	7.032027E-4	45.000004	16
CACTGCG	20	7.032027E-4	45.000004	37
TAACGAT	20	7.032027E-4	45.000004	11
GAACGTT	20	7.032027E-4	45.000004	15
ACCGTAG	20	7.032027E-4	45.000004	17
GGCGCGT	20	7.032027E-4	45.000004	29
TTGTTCG	60	0.0	45.000004	1
TACCGAA	20	7.032027E-4	45.000004	25
TCGTTAG	75	0.0	45.0	1
TCTACGG	210	0.0	42.857143	2
TAGGGCG	375	0.0	42.6	5
AATACGG	175	0.0	42.42857	5
CGTATGG	150	0.0	42.0	2
ACCGAAC	60	3.6379788E-12	41.250004	11
TAATACG	180	0.0	41.25	4
TATGGGA	1255	0.0	41.23506	4
CACGGGT	55	6.002665E-11	40.909092	4
TTCGTAG	55	6.002665E-11	40.909092	1
TCGTCAG	55	6.002665E-11	40.909092	1
TTGCGAG	105	0.0	40.714287	1