FastQCFastQC Report
Sat 14 Jan 2017
SRR2934107.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934107.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences695704
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC60620.871347584604947No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT52120.7491691868955763No Hit
TCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC38600.5548336648919655No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC33840.486413762174718No Hit
CCTGTATCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC20950.301133815530743No Hit
CTGTCACTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT19650.28244770764578037No Hit
TCTGTATCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC16660.23946965951036647No Hit
GCTGTATCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC14220.204397272403206No Hit
TCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG13730.19735404712348928No Hit
CGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG11080.15926313489645022No Hit
CTGTATCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT9750.14014580913721927No Hit
GCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG9650.13870841622299138No Hit
CCTGACTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC9590.13784598047445465No Hit
TCTGACTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC9080.1305152766118924No Hit
TGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG8270.11887239400664651No Hit
GCTGACTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC7870.11312282234973495No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC7700.11067925439554754No Hit
TTCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT7340.10550463990432714No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC7230.10392350769867645No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAA253.8892118E-545.00000430
AAACTCG207.031364E-445.00000412
CGGGTAC600.045.0000046
TAATACG253.8892118E-545.0000044
CTGTCGT207.031364E-445.00000431
ACGCCTA207.031364E-445.00000439
GACGTTA207.031364E-445.0000049
ATTACCG302.1642609E-645.00000419
CGTCTAT207.031364E-445.0000041
TAAGTGT1600.045.00000425
GTAATCG207.031364E-445.00000435
ACGATGA207.031364E-445.00000429
TAACCGT253.8892118E-545.00000412
CCATCGT207.031364E-445.00000433
TCTCGAG302.1642609E-645.0000041
AACGCGA253.8892118E-545.00000427
GTCGTAA207.031364E-445.00000432
TTGCGAT207.031364E-445.0000041
GTATCCG253.8892118E-545.00000427
CGTAATC207.031364E-445.00000434
Produced by FastQC (version 0.11.5)