##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934107.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	695704
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.755030875199797	31.0	31.0	34.0	30.0	34.0
2	31.965992721042284	33.0	31.0	34.0	30.0	34.0
3	32.043800236882355	33.0	31.0	34.0	30.0	34.0
4	35.56565292135736	37.0	35.0	37.0	33.0	37.0
5	29.320864908064348	35.0	33.0	37.0	0.0	37.0
6	23.606558248910456	35.0	10.0	37.0	0.0	37.0
7	30.296745454963606	35.0	19.0	37.0	17.0	37.0
8	34.09938422087554	35.0	32.0	37.0	32.0	37.0
9	36.66903021974863	37.0	35.0	39.0	33.0	39.0
10	36.78784943021745	38.0	37.0	39.0	33.0	39.0
11	36.99773898094592	39.0	37.0	39.0	33.0	39.0
12	37.07406741947725	39.0	37.0	39.0	34.0	39.0
13	36.9857956832216	39.0	37.0	39.0	33.0	39.0
14	38.03569046606028	40.0	37.0	41.0	33.0	41.0
15	37.9014077826202	40.0	37.0	41.0	33.0	41.0
16	38.13254200062095	40.0	37.0	41.0	34.0	41.0
17	38.12304945781539	40.0	37.0	41.0	34.0	41.0
18	38.22540620723756	40.0	37.0	41.0	34.0	41.0
19	38.24458677828502	40.0	37.0	41.0	34.0	41.0
20	38.21489742764164	40.0	37.0	41.0	34.0	41.0
21	38.100376021986364	40.0	37.0	41.0	34.0	41.0
22	38.16255907684877	40.0	37.0	41.0	34.0	41.0
23	38.11670911767073	40.0	37.0	41.0	34.0	41.0
24	38.09526321539045	40.0	37.0	41.0	34.0	41.0
25	37.87345911479595	40.0	37.0	41.0	33.0	41.0
26	37.744144061267434	40.0	37.0	41.0	33.0	41.0
27	36.85689891103113	39.0	36.0	41.0	32.0	41.0
28	37.122498936329244	39.0	36.0	41.0	31.0	41.0
29	37.42495371594816	39.0	36.0	41.0	32.0	41.0
30	37.48891338845256	39.0	36.0	40.0	33.0	41.0
31	37.43840915101825	39.0	36.0	40.0	33.0	41.0
32	37.43179570621989	39.0	36.0	40.0	33.0	41.0
33	36.30521888619298	39.0	35.0	40.0	31.0	41.0
34	36.74952278555248	39.0	35.0	40.0	31.0	41.0
35	37.08607683727562	39.0	35.0	40.0	31.0	41.0
36	37.226944217655785	39.0	35.0	40.0	32.0	41.0
37	37.18870956613732	39.0	35.0	40.0	32.0	41.0
38	37.077231121281464	39.0	35.0	40.0	32.0	41.0
39	37.22463432724262	39.0	35.0	40.0	32.0	41.0
40	37.234556650529534	39.0	35.0	40.0	32.0	41.0
41	37.18746622126652	39.0	35.0	40.0	32.0	41.0
42	37.201738095511885	39.0	35.0	40.0	32.0	41.0
43	37.00831675540172	39.0	35.0	40.0	32.0	41.0
44	36.956909835217274	39.0	35.0	40.0	31.0	41.0
45	36.97652737371066	39.0	35.0	40.0	32.0	41.0
46	36.946815024780655	39.0	35.0	40.0	32.0	41.0
47	36.87057714200292	39.0	35.0	40.0	31.0	41.0
48	36.7911180617044	39.0	35.0	40.0	31.0	41.0
49	36.814112036153304	39.0	35.0	40.0	32.0	41.0
50	36.660332555224635	39.0	35.0	40.0	32.0	41.0
51	35.832001828363786	38.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	4.0
11	7.0
12	5.0
13	13.0
14	8.0
15	25.0
16	28.0
17	48.0
18	81.0
19	154.0
20	238.0
21	431.0
22	717.0
23	1131.0
24	1740.0
25	2540.0
26	3604.0
27	4721.0
28	6248.0
29	8476.0
30	11296.0
31	16596.0
32	23961.0
33	36957.0
34	50561.0
35	67029.0
36	91048.0
37	132377.0
38	138921.0
39	96705.0
40	31.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.830393385692766	4.31835953221485	53.186843830134656	18.66440325195773
2	19.758977956142267	4.71105527638191	51.98403343950876	23.545933327967067
3	20.081960143969273	4.800892333521153	52.38204753745846	22.735099985051114
4	20.52855812241988	4.607562986557501	49.83843703644079	25.025441854581832
5	14.660545289375943	22.712532916297736	40.0096880282419	22.61723376608443
6	15.949311776272667	29.298523509998503	39.94083690765038	14.811327806078447
7	76.92006945482561	2.382909973206996	13.530035762335707	7.166984809631682
8	75.3771719006934	3.1036187804008604	11.674936467233191	9.84427285167255
9	68.4831480054736	6.112800846336948	16.168082977818152	9.235968170371308
10	34.164529742534185	26.986908225337213	22.456820716856594	16.391741315272014
11	25.320538619872817	25.203678575946093	28.030311741775236	21.44547106240585
12	24.509417798374024	19.708669204144293	34.65036854754321	21.13154444993848
13	22.623989512781296	23.708214987983396	33.77614617710981	19.891649322125502
14	17.88720490323471	26.432936996193785	31.94059542564079	23.739262674930718
15	15.086444809861666	25.03507238710716	37.80688338718765	22.071599415843522
16	16.528437381415085	23.656325103779768	33.5099410093948	26.305296505410347
17	15.330226648114714	24.806526913744925	30.840702367673607	29.02254407046675
18	17.066453549210582	22.913049227832527	34.74926118004208	25.2712360429148
19	19.748916205742674	24.905994503409495	31.48566056828766	23.859428722560168
20	22.660499292802687	25.36955371824799	31.27005163115348	20.699895357795846
21	21.633482055586857	26.841731541000197	29.996952727021835	21.527833676391108
22	18.36945022595817	25.036078562147118	29.12589262099974	27.468578590894978
23	18.587358991755114	25.5702137690742	27.899940204454772	27.942487034715914
24	21.559456320504122	22.82666191368743	29.153346825661487	26.460534940146957
25	19.815180019088576	24.90570702482665	26.630578521900116	28.648534434184654
26	21.762847417867366	25.20468475098605	28.214872991962096	24.81759483918448
27	23.09243586352817	27.429625242919403	27.219622138150708	22.258316755401722
28	18.85715764175569	27.436668468199123	29.466123523797478	24.240050366247715
29	20.65246139162632	25.854098869634214	27.66966985959546	25.823769879144002
30	23.149500362223012	25.509987005968053	26.5522406080747	24.78827202373423
31	20.624863447673146	30.854932557524467	25.057495716569118	23.462708278233272
32	22.03077745707945	31.65857893586928	23.22237618297437	23.088267424076907
33	23.93733541851132	28.089532329841425	23.372008785345493	24.60112346630176
34	20.48471763853593	25.75707484792383	27.937024941641848	25.821182571898394
35	22.829536699515888	25.21733380863126	24.92266826121454	27.03046123063832
36	27.21372882720237	24.75075606867288	24.983326242194956	23.052188861929785
37	21.857571610914984	29.247496061543416	28.7592136885802	20.135718638961396
38	18.832147005048125	32.98069293837609	25.036653519312814	23.150506537262974
39	21.20887618872394	25.004168439451263	28.527793429389508	25.259161942435288
40	22.58589860055426	22.154968204868737	30.98372296263928	24.275410231937723
41	21.101646677322538	24.69167921989812	27.070420753653853	27.13625334912549
42	26.31305842714718	24.261611259961132	25.194766739877878	24.23056357301381
43	27.332744960500442	22.755223485850305	26.45852259006704	23.453508963582212
44	21.41758563986983	23.436547727194323	27.17808148292952	27.967785150006325
45	22.67242965399078	20.780964318158297	26.468584340466634	30.078021687384286
46	23.55383898899532	21.943958925060084	30.13867966836471	24.36352241757989
47	18.93880155928383	24.24766854869312	32.594321723031634	24.21920816899141
48	19.60287708565712	23.430366937663145	30.614887940848405	26.351868035831334
49	23.02861561813645	19.970130975242345	32.91629773581868	24.084955670802525
50	22.336798408518565	19.876987914400377	29.50407069673309	28.282142980347963
51	19.779245196232882	18.980485953796443	26.908139093637523	34.33212975633315
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	253.0
1	460.5
2	668.0
3	1249.5
4	1831.0
5	1373.5
6	916.0
7	958.5
8	1001.0
9	1185.0
10	1369.0
11	1469.5
12	1570.0
13	1525.0
14	1480.0
15	1450.5
16	1421.0
17	1381.0
18	1341.0
19	1451.5
20	1562.0
21	1673.0
22	1784.0
23	1944.0
24	2104.0
25	2486.0
26	3588.0
27	4308.0
28	4773.5
29	5239.0
30	6382.5
31	7526.0
32	8895.5
33	10265.0
34	11622.0
35	12979.0
36	14230.0
37	15481.0
38	17815.5
39	20150.0
40	23994.0
41	27838.0
42	33780.5
43	39723.0
44	48794.5
45	57866.0
46	72993.0
47	88120.0
48	101136.5
49	114153.0
50	104666.0
51	95179.0
52	75369.0
53	55559.0
54	44755.5
55	33952.0
56	28345.0
57	22738.0
58	20824.5
59	18911.0
60	16822.5
61	14734.0
62	12688.5
63	10643.0
64	8821.5
65	7000.0
66	5906.0
67	4812.0
68	3999.0
69	3186.0
70	2648.5
71	2111.0
72	1770.5
73	1430.0
74	1099.5
75	568.5
76	368.0
77	297.5
78	227.0
79	175.5
80	124.0
81	106.0
82	88.0
83	67.0
84	46.0
85	28.0
86	10.0
87	5.0
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	695704.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.11024425913216
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.9230333834932	28.74552981734858
2	13.122251974749465	10.789179678236598
3	5.3623581670194485	6.613435621533652
4	2.919180661095545	4.80032920056691
5	1.831636281165563	3.764950745630238
6	1.2836707986534317	3.1663212048574696
7	0.9111682145791244	2.622084350375361
8	0.7381508702749267	2.427645006167457
9	0.6006645562467462	2.222411996259627
>10	3.12541439559963	21.052012397509905
>50	0.11146899618919355	3.1658693500540314
>100	0.06217247387460611	4.760524834772259
>500	0.00515038245115078	1.612804169330344
>1k	0.002943075686371874	2.551842155768089
>5k	7.357689215929685E-4	1.705059471589488
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	6062	0.871347584604947	No Hit
CTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	5212	0.7491691868955763	No Hit
TCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	3860	0.5548336648919655	No Hit
GCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	3384	0.486413762174718	No Hit
CCTGTATCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	2095	0.301133815530743	No Hit
CTGTCACTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	1965	0.28244770764578037	No Hit
TCTGTATCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	1666	0.23946965951036647	No Hit
GCTGTATCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	1422	0.204397272403206	No Hit
TCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	1373	0.19735404712348928	No Hit
CGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	1108	0.15926313489645022	No Hit
CTGTATCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGCT	975	0.14014580913721927	No Hit
GCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	965	0.13870841622299138	No Hit
CCTGACTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	959	0.13784598047445465	No Hit
TCTGACTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	908	0.1305152766118924	No Hit
TGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTG	827	0.11887239400664651	No Hit
GCTGACTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCTGC	787	0.11312282234973495	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCC	770	0.11067925439554754	No Hit
TTCCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGCCGTCTTCT	734	0.10550463990432714	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGAACTAGTCGTATGC	723	0.10392350769867645	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.8053712498418868	0.0
2	0.0	0.0	0.0	2.8949093292549706	0.0
3	0.0	0.0	0.0	3.9350068419902717	0.0
4	0.0	0.0	0.0	5.0160700527810675	0.0
5	0.0	0.0	0.0	6.7468348608028705	0.0
6	0.0	0.0	0.0	7.994060692478411	0.0
7	0.0	0.0	0.0	9.617595989098811	0.0
8	0.0	0.0	0.0	12.189810609109621	0.0
9	0.0	0.0	0.0	13.330525625840874	0.0
10	0.0	0.0	0.0	15.49897657624507	0.0
11	0.0	0.0	0.0	19.41443487460184	0.0
12	0.0	0.0	0.0	22.934753860837368	0.0
13	0.0	0.0	0.0	24.097892207030576	0.0
14	0.0	0.0	0.0	24.495618826397433	0.0
15	0.0	0.0	0.0	25.114128997389695	0.0
16	0.0	0.0	0.0	26.497044720168347	0.0
17	0.0	0.0	0.0	28.432063061301932	0.0
18	0.0	0.0	0.0	30.690638547428218	0.0
19	0.0	0.0	0.0	32.03963179743109	0.0
20	0.0	0.0	0.0	33.43217805273507	0.0
21	0.0	0.0	0.0	35.204627255269486	0.0
22	0.0	0.0	0.0	36.94904729597645	0.0
23	0.0	0.0	0.0	38.56726423881421	0.0
24	0.0	0.0	0.0	39.815352506238284	0.0
25	0.0	0.0	0.0	40.8384887825857	0.0
26	0.0	0.0	0.0	41.7013557489967	0.0
27	1.43739291422789E-4	0.0	0.0	42.54984878626542	0.0
28	1.43739291422789E-4	0.0	0.0	43.455981279394685	0.0
29	1.43739291422789E-4	0.0	0.0	44.48199234157055	0.0
30	1.43739291422789E-4	0.0	0.0	45.55256258408748	0.0
31	1.43739291422789E-4	0.0	0.0	46.51475340087163	0.0
32	1.43739291422789E-4	0.0	0.0	47.434253648103216	0.0
33	1.43739291422789E-4	0.0	0.0	48.309338454285154	0.0
34	1.43739291422789E-4	0.0	0.0	49.162431148879406	0.0
35	1.43739291422789E-4	0.0	0.0	50.032628819152976	0.0
36	1.43739291422789E-4	0.0	0.0	50.846911905063074	0.0
37	1.43739291422789E-4	0.0	0.0	51.65530168002484	0.0
38	1.43739291422789E-4	0.0	0.0	52.439543254027576	0.0
39	1.43739291422789E-4	0.0	0.0	53.22694709244161	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAA	25	3.8892118E-5	45.000004	30
AAACTCG	20	7.031364E-4	45.000004	12
CGGGTAC	60	0.0	45.000004	6
TAATACG	25	3.8892118E-5	45.000004	4
CTGTCGT	20	7.031364E-4	45.000004	31
ACGCCTA	20	7.031364E-4	45.000004	39
GACGTTA	20	7.031364E-4	45.000004	9
ATTACCG	30	2.1642609E-6	45.000004	19
CGTCTAT	20	7.031364E-4	45.000004	1
TAAGTGT	160	0.0	45.000004	25
GTAATCG	20	7.031364E-4	45.000004	35
ACGATGA	20	7.031364E-4	45.000004	29
TAACCGT	25	3.8892118E-5	45.000004	12
CCATCGT	20	7.031364E-4	45.000004	33
TCTCGAG	30	2.1642609E-6	45.000004	1
AACGCGA	25	3.8892118E-5	45.000004	27
GTCGTAA	20	7.031364E-4	45.000004	32
TTGCGAT	20	7.031364E-4	45.000004	1
GTATCCG	25	3.8892118E-5	45.000004	27
CGTAATC	20	7.031364E-4	45.000004	34
>>END_MODULE