##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934106.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1366920
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.025482837327715	33.0	31.0	34.0	30.0	34.0
2	32.23942293623621	34.0	31.0	34.0	30.0	34.0
3	32.31835732888538	34.0	31.0	34.0	30.0	34.0
4	35.72105170748837	37.0	35.0	37.0	35.0	37.0
5	29.581219822667016	37.0	35.0	37.0	0.0	37.0
6	23.681570977087173	35.0	10.0	37.0	0.0	37.0
7	30.391515231322973	35.0	19.0	37.0	17.0	37.0
8	34.30329353583238	35.0	32.0	37.0	32.0	37.0
9	36.961708805197084	38.0	37.0	39.0	34.0	39.0
10	37.05618178093817	39.0	37.0	39.0	34.0	39.0
11	37.21012275773271	39.0	37.0	39.0	34.0	39.0
12	37.16074020425482	39.0	37.0	39.0	34.0	39.0
13	37.10501711877798	39.0	37.0	39.0	33.0	39.0
14	38.1437143358792	40.0	38.0	41.0	33.0	41.0
15	38.044286424955374	40.0	37.0	41.0	33.0	41.0
16	38.29210341497674	40.0	37.0	41.0	33.0	41.0
17	38.28620109443128	40.0	37.0	41.0	34.0	41.0
18	38.35088154390893	40.0	37.0	41.0	34.0	41.0
19	38.35313697948673	40.0	37.0	41.0	34.0	41.0
20	38.28879012670822	40.0	37.0	41.0	34.0	41.0
21	38.16741799081146	40.0	37.0	41.0	34.0	41.0
22	38.26800324817839	40.0	37.0	41.0	34.0	41.0
23	38.2165766833465	40.0	37.0	41.0	34.0	41.0
24	38.20320062622538	40.0	37.0	41.0	34.0	41.0
25	37.969006964562666	40.0	36.0	41.0	34.0	41.0
26	37.85374711029175	40.0	36.0	41.0	33.0	41.0
27	37.01512963450678	40.0	36.0	41.0	33.0	41.0
28	37.384473853627135	40.0	36.0	41.0	33.0	41.0
29	37.655935972844055	40.0	36.0	41.0	33.0	41.0
30	37.647235390512975	40.0	36.0	41.0	33.0	41.0
31	37.678419366166274	40.0	36.0	41.0	33.0	41.0
32	37.67549307933164	40.0	36.0	41.0	33.0	41.0
33	36.47675942995932	40.0	35.0	41.0	31.0	41.0
34	36.917544552717054	40.0	35.0	41.0	31.0	41.0
35	37.176230503613965	40.0	35.0	41.0	31.0	41.0
36	37.267467737687646	39.0	35.0	41.0	32.0	41.0
37	37.37990079887631	39.0	35.0	41.0	33.0	41.0
38	37.32250387733006	39.0	35.0	41.0	33.0	41.0
39	37.33629254089486	39.0	35.0	41.0	33.0	41.0
40	37.27365829748632	39.0	35.0	41.0	33.0	41.0
41	37.14830641149445	39.0	35.0	41.0	33.0	41.0
42	37.16235844087437	39.0	35.0	41.0	33.0	41.0
43	37.071597459983025	39.0	35.0	41.0	33.0	41.0
44	37.118554853246714	39.0	35.0	41.0	33.0	41.0
45	37.03262736663448	39.0	35.0	41.0	33.0	41.0
46	36.95314136891698	39.0	35.0	40.0	32.0	41.0
47	36.86829660843356	39.0	35.0	40.0	32.0	41.0
48	36.78964460246393	39.0	35.0	40.0	32.0	41.0
49	36.79756313463846	39.0	35.0	40.0	32.0	41.0
50	36.70538729406256	38.0	35.0	40.0	32.0	41.0
51	35.89293594358119	37.0	34.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	18.0
11	23.0
12	13.0
13	27.0
14	22.0
15	28.0
16	65.0
17	109.0
18	213.0
19	360.0
20	634.0
21	1183.0
22	1898.0
23	2702.0
24	4043.0
25	5632.0
26	7480.0
27	9441.0
28	11725.0
29	15551.0
30	21150.0
31	30025.0
32	43192.0
33	71297.0
34	97697.0
35	119991.0
36	159055.0
37	240137.0
38	279861.0
39	243202.0
40	140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.979896409446052	4.228703947560939	54.190223275685476	17.601176367307524
2	19.319857782459838	5.192037573522957	53.731454657185495	21.756649986831707
3	21.48618792614052	5.287141895648611	51.49789307347906	21.728777104731805
4	18.689974541304537	4.687472566060926	51.948614403183804	24.673938489450737
5	15.523512714716297	22.260263951072485	41.2160184941328	21.000204840078425
6	16.4630702601469	29.989392210224448	39.36682468615574	14.180712843472918
7	84.31195680800631	1.9919234483364059	9.14706054487461	4.549059198782665
8	86.10496590875837	2.7931407836596143	6.5210107394726835	4.580882568109327
9	81.14469025253855	4.650893980627981	8.60116173587335	5.603254030960115
10	41.46680127586106	27.280016387206274	15.19591490357885	16.057267433353818
11	30.437553038948877	22.885830919146695	24.96349457173792	21.713121470166506
12	26.18675562578644	20.38875720598133	30.659219266672523	22.76526790155971
13	25.17235829456003	20.87042401896234	32.522605565797555	21.434612120680068
14	21.153395955871595	22.80587013139028	33.38629912504024	22.65443478769789
15	19.216340385684603	21.8765545898809	35.40221812542065	23.50488689901384
16	24.187004360167382	21.892210224446202	29.814546571854972	24.106238843531443
17	24.315395195037016	22.092148772422675	28.856041319170107	24.736414713370205
18	24.91601556784596	21.32546162174816	29.823983846896674	23.9345389635092
19	25.640564188101717	23.36698563193164	27.94230825505516	23.05014192491148
20	26.1310830187575	25.84123430778685	27.52355660901882	20.504126064436836
21	26.11806104234337	25.35569016474995	28.280440698797292	20.245808094109385
22	24.91747871126328	21.4978199163082	29.609706493430487	23.97499487899804
23	23.56882626635063	23.219500775466013	29.197026892576012	24.01464606560735
24	24.33097767243145	23.597430720159192	28.141661545664704	23.929930061744653
25	25.21032686623943	23.88193895765663	25.346838147075175	25.56089602902877
26	23.032437889561937	24.07763438972288	26.657522020308434	26.23240570040675
27	21.846413835484153	24.808474526673105	28.676733093377816	24.66837854446493
28	21.2657653703216	23.452579521844733	28.766131156175927	26.515523951657745
29	22.983495742252654	23.34072220759079	28.012831767769875	25.66295028238668
30	25.385830919146695	23.256591461095017	28.469625142656486	22.887952477101805
31	26.119670500102423	23.845214057881954	26.641939542913995	23.393175899101628
32	26.080458256518302	23.913542855470695	25.772978667368974	24.233020220642025
33	25.065402510754105	26.527814356363212	24.58205308284318	23.824730050039506
34	24.21502355660902	23.746232405700407	28.088549439616074	23.950194598074503
35	23.34855002487344	24.82171597459983	28.02826793082258	23.80146606970415
36	25.501199777602203	26.59921575512832	26.306952857519093	21.592631609750388
37	24.486802446375794	25.83538173411758	27.12901998653908	22.548795832967546
38	24.51730898662687	25.94102068884792	25.698504667427503	23.84316565709771
39	23.804392356538788	25.203011149152836	25.410192257045033	25.582404237263333
40	25.45891493284172	23.702264946010008	27.558964679717906	23.27985544143037
41	23.16404763994967	23.996356772890877	27.223904837152133	25.615690750007314
42	22.600810581453192	23.97155649196734	28.85238346062681	24.575249465952652
43	23.109838176338044	23.402540016972466	28.179849588856698	25.30777221783279
44	23.519225704503555	22.308035583647907	27.73929710590232	26.433441605946218
45	23.958315044040617	21.35538290463231	26.64845053112106	28.037851520206008
46	24.25328475697188	22.423184970590817	28.384762824452054	24.938767447985253
47	21.53922687501829	24.256576829660844	30.399511310098614	23.804684985222252
48	21.86053316946127	24.651259766482312	29.016474994878998	24.47173206917742
49	24.24150645246247	22.682307669797794	29.26959880607497	23.806587071664765
50	23.242911070143098	22.543162730810874	27.899511310098617	26.31441488894741
51	21.878602990665144	22.380388025634275	27.17810844819009	28.562900535510487
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	263.0
1	740.0
2	1217.0
3	2677.0
4	4137.0
5	2958.0
6	1779.0
7	1753.0
8	1727.0
9	1753.0
10	1779.0
11	1868.0
12	1957.0
13	1899.0
14	1841.0
15	1803.0
16	1765.0
17	1865.0
18	1965.0
19	2146.5
20	2328.0
21	2632.5
22	2937.0
23	3587.5
24	4238.0
25	5100.5
26	7746.5
27	9530.0
28	11486.5
29	13443.0
30	15956.0
31	18469.0
32	21185.5
33	23902.0
34	26401.0
35	28900.0
36	32026.5
37	35153.0
38	38840.0
39	42527.0
40	46833.5
41	51140.0
42	57467.5
43	63795.0
44	73762.5
45	83730.0
46	100857.0
47	117984.0
48	129394.5
49	140805.0
50	138316.0
51	135827.0
52	123547.0
53	111267.0
54	102373.0
55	93479.0
56	88313.0
57	83147.0
58	77934.0
59	72721.0
60	66302.0
61	59883.0
62	52877.5
63	45872.0
64	39960.0
65	34048.0
66	28712.5
67	23377.0
68	19160.5
69	14944.0
70	13149.5
71	11355.0
72	9730.5
73	8106.0
74	6577.0
75	3819.5
76	2591.0
77	1854.0
78	1117.0
79	812.0
80	507.0
81	374.0
82	241.0
83	170.0
84	99.0
85	56.0
86	13.0
87	8.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1366920.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.28438050798746
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.1315821819093	25.809653940235776
2	10.339381750950627	7.503161233376683
3	3.9677498867717667	4.319020399564528
4	2.3545488293043904	3.417333825884677
5	1.665773073684752	3.0220772022768685
6	1.3299728790558236	2.895434520537905
7	1.1549214926044473	2.933392762615881
8	0.9617600681208636	2.7917494615268277
9	0.8234126684839339	2.688931672053155
>10	6.157787854731018	38.64044036204319
>50	0.07616112635712664	1.8865966399492298
>100	0.032469620630851855	2.0616636954331624
>500	0.0022392836975686325	0.5307316740573702
>1k	0.0022392836975686325	1.4998126104447331
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	3404	0.24902700962748367	TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGCT	2840	0.2077663652591227	TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	1948	0.14251016884675036	TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGC	1908	0.13958388201211483	No Hit
TCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTCTGC	1716	0.12553770520586427	TruSeq Adapter, Index 15 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCGTCTTC	1563	0.11434465806338337	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCAGTATCGTATGCCG	1522	0.11134521405788195	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.315717086588827E-5	0.0	0.0	0.2233488426535569	0.0
2	7.315717086588827E-5	0.0	0.0	0.787098001346092	0.0
3	7.315717086588827E-5	0.0	0.0	1.0739472683112399	0.0
4	7.315717086588827E-5	0.0	0.0	1.4368068358060457	0.0
5	7.315717086588827E-5	0.0	0.0	2.0950019020864423	0.0
6	7.315717086588827E-5	0.0	0.0	2.6248792906680714	0.0
7	7.315717086588827E-5	0.0	0.0	3.296242647704328	0.0
8	7.315717086588827E-5	0.0	0.0	4.354095338425072	0.0
9	7.315717086588827E-5	0.0	0.0	4.827714862610833	0.0
10	7.315717086588827E-5	0.0	0.0	5.783440142802798	0.0
11	7.315717086588827E-5	0.0	0.0	7.418649225997132	0.0
12	7.315717086588827E-5	0.0	0.0	9.132209639188833	0.0
13	7.315717086588827E-5	0.0	0.0	9.632458373569778	0.0
14	1.4631434173177654E-4	0.0	0.0	9.81425394317151	0.0
15	1.4631434173177654E-4	0.0	0.0	10.087349662013871	0.0
16	1.4631434173177654E-4	0.0	0.0	10.649855148801686	0.0
17	1.4631434173177654E-4	0.0	0.0	11.423126444854125	0.0
18	1.4631434173177654E-4	0.0	0.0	12.303353524712492	0.0
19	1.4631434173177654E-4	0.0	0.0	12.976911596874725	0.0
20	1.4631434173177654E-4	0.0	0.0	13.6716120914172	0.0
21	1.4631434173177654E-4	0.0	0.0	14.612998566119451	0.0
22	1.4631434173177654E-4	0.0	0.0	15.589573640008194	0.0
23	1.4631434173177654E-4	0.0	0.0	16.492625757176718	0.0
24	1.4631434173177654E-4	0.0	0.0	17.187838351915254	0.0
25	1.4631434173177654E-4	0.0	0.0	17.792628683463555	0.0
26	1.4631434173177654E-4	0.0	0.0	18.33801539226875	0.0
27	1.4631434173177654E-4	0.0	0.0	18.896936177684136	0.0
28	1.4631434173177654E-4	0.0	0.0	19.472390483715213	0.0
29	1.4631434173177654E-4	0.0	0.0	20.109369970444504	0.0
30	1.4631434173177654E-4	0.0	0.0	20.84086852193252	0.0
31	1.4631434173177654E-4	0.0	0.0	21.455827700231175	0.0
32	1.4631434173177654E-4	0.0	0.0	22.019576858923713	0.0
33	2.1947151259766484E-4	0.0	0.0	22.616466216018495	0.0
34	2.1947151259766484E-4	0.0	0.0	23.2641266496942	0.0
35	2.1947151259766484E-4	0.0	0.0	23.988748427120825	0.0
36	2.1947151259766484E-4	0.0	0.0	24.61731483920054	0.0
37	2.1947151259766484E-4	0.0	0.0	25.24763702338103	0.0
38	2.1947151259766484E-4	0.0	0.0	25.97057618587774	0.0
39	2.926286834635531E-4	0.0	0.0	27.07817575278729	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATCGA	25	3.8911945E-5	45.0	41
TATCGCG	25	3.8911945E-5	45.0	32
CGATAAT	20	7.033756E-4	45.0	10
TACGTCA	20	7.033756E-4	45.0	38
TAATACG	105	0.0	42.85714	4
CCGTTAG	75	0.0	42.000004	1
CGATAAC	70	0.0	41.785717	16
CGTTAGG	420	0.0	41.249996	2
TAGGGCG	665	0.0	40.93985	5
TCGTTAG	165	0.0	40.909092	1
GGCGATA	55	6.184564E-11	40.909092	8
TTACGGG	1375	0.0	40.581818	3
TTAGGGA	2870	0.0	40.452965	4
TTATGGG	3890	0.0	40.43059	3
CGTATGG	245	0.0	40.408165	2
TACCGAT	45	1.9286745E-8	40.000004	29
TATGGGA	2325	0.0	39.870968	4
TAGGGAT	1575	0.0	39.857143	5
CTATGGG	1860	0.0	39.67742	3
TAGGGCA	675	0.0	39.666668	5
>>END_MODULE