##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934105.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	785270
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.871267207457308	33.0	31.0	34.0	30.0	34.0
2	32.0812026436767	33.0	31.0	34.0	30.0	34.0
3	32.17493982961275	34.0	31.0	34.0	30.0	34.0
4	35.63635182803367	37.0	35.0	37.0	33.0	37.0
5	29.520549619875965	37.0	35.0	37.0	0.0	37.0
6	23.719602175048074	35.0	10.0	37.0	0.0	37.0
7	30.387150916245368	35.0	19.0	37.0	17.0	37.0
8	34.22744915761458	35.0	32.0	37.0	32.0	37.0
9	36.80317979803125	38.0	37.0	39.0	34.0	39.0
10	36.91763724578807	39.0	37.0	39.0	33.0	39.0
11	37.13880958141786	39.0	37.0	39.0	34.0	39.0
12	37.184831968622255	39.0	37.0	39.0	34.0	39.0
13	37.12072280871547	39.0	37.0	39.0	33.0	39.0
14	38.24149910221962	40.0	38.0	41.0	34.0	41.0
15	38.10012607128758	40.0	37.0	41.0	33.0	41.0
16	38.30100092961656	40.0	37.0	41.0	34.0	41.0
17	38.32647497039235	40.0	37.0	41.0	34.0	41.0
18	38.39570720898544	40.0	38.0	41.0	34.0	41.0
19	38.40423803277854	40.0	38.0	41.0	34.0	41.0
20	38.36664459357928	40.0	37.0	41.0	34.0	41.0
21	38.23635564837572	40.0	37.0	41.0	34.0	41.0
22	38.287308823716685	40.0	37.0	41.0	34.0	41.0
23	38.244371999439686	40.0	37.0	41.0	34.0	41.0
24	38.239052809861576	40.0	37.0	41.0	34.0	41.0
25	37.98593222713207	40.0	37.0	41.0	34.0	41.0
26	37.851920995326445	40.0	37.0	41.0	33.0	41.0
27	36.98600863397303	40.0	36.0	41.0	32.0	41.0
28	37.38714709590332	40.0	36.0	41.0	32.0	41.0
29	37.68255249786698	40.0	36.0	41.0	33.0	41.0
30	37.67318629261273	39.0	36.0	41.0	33.0	41.0
31	37.660328294726654	39.0	36.0	41.0	33.0	41.0
32	37.670969220777565	40.0	36.0	41.0	33.0	41.0
33	36.508372916321775	39.0	36.0	41.0	31.0	41.0
34	36.96737427891044	39.0	36.0	41.0	31.0	41.0
35	37.26904504183275	39.0	36.0	41.0	31.0	41.0
36	37.375851617914854	39.0	36.0	41.0	33.0	41.0
37	37.42687865320208	39.0	36.0	41.0	33.0	41.0
38	37.410309829740086	39.0	36.0	41.0	33.0	41.0
39	37.45577444700549	39.0	36.0	41.0	33.0	41.0
40	37.33243852432921	39.0	36.0	41.0	33.0	41.0
41	37.24023456900174	39.0	35.0	41.0	33.0	41.0
42	37.30509760973933	39.0	35.0	41.0	33.0	41.0
43	37.18789460949737	39.0	35.0	41.0	32.0	41.0
44	37.19068091229768	39.0	35.0	40.0	32.0	41.0
45	37.15962153144778	39.0	35.0	40.0	32.0	41.0
46	37.10361149668267	39.0	35.0	40.0	32.0	41.0
47	37.04925567002432	39.0	35.0	40.0	32.0	41.0
48	37.00810167203637	39.0	35.0	40.0	32.0	41.0
49	37.01836056388249	39.0	35.0	40.0	32.0	41.0
50	36.9114100882499	39.0	35.0	40.0	32.0	41.0
51	36.07850802908554	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	7.0
10	7.0
11	8.0
12	10.0
13	8.0
14	13.0
15	20.0
16	35.0
17	61.0
18	70.0
19	147.0
20	287.0
21	420.0
22	770.0
23	1225.0
24	1889.0
25	2762.0
26	3837.0
27	4913.0
28	6435.0
29	8755.0
30	12289.0
31	17212.0
32	24547.0
33	38667.0
34	53729.0
35	72299.0
36	96484.0
37	145320.0
38	162718.0
39	130249.0
40	74.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.219822481439504	4.714047397710342	53.07397455652196	17.992155564328193
2	19.769888063977994	4.643498414558049	53.118163179543345	22.46845034192061
3	21.258420670597374	4.2284819234148765	51.902402995148165	22.610694410839585
4	20.13231117959428	4.654450061762196	50.15064882142448	25.062589937219045
5	15.513517643613026	22.234263374380784	40.731595502183964	21.520623479822227
6	16.402383893437925	29.14615355228138	39.62178613725216	14.829676417028537
7	80.76457778853133	2.1983521591299806	10.939931488532606	6.097138563806079
8	80.72548295490722	2.658321341704127	9.271460771454404	7.34473493193424
9	73.66103378455817	6.121588752913011	12.805022476345714	7.412354986183097
10	34.83094986437786	30.511161766016787	18.75253097660677	15.905357392998587
11	27.76535459141442	24.686668279699976	26.934939574923273	20.613037553962332
12	26.228558330255836	20.053612133406347	32.012938225069085	21.70489131126874
13	23.981433137646924	23.03373362028347	31.471086377933705	21.513746864135904
14	19.38734448024246	25.806410533956477	31.00857030066092	23.797674685140144
15	17.007398729099545	25.285697912819792	35.62952869713602	22.077374660944642
16	18.34146217224649	23.5040177263871	33.37056044417844	24.783959657187975
17	17.909763520827234	23.853833713245127	30.625389993250728	27.61101277267691
18	18.89120939294765	23.109249048098107	32.68671921759395	25.312822341360295
19	21.783972391661464	24.64158824353407	30.601449183083524	22.972990181720938
20	25.00872311434284	25.28404243126568	29.44197537152826	20.265259082863217
21	23.70331223655558	25.35726565385154	28.828555783361136	22.110866326231744
22	20.793739732830748	25.42781463700383	27.42330663338724	26.355138996778177
23	21.010098437480103	25.422466158136693	27.44291772256676	26.124517681816446
24	22.754975995517466	22.48271295223299	28.529677690475886	26.232633361773654
25	21.333554064207217	25.073414239688258	26.718580870273918	26.874450825830603
26	21.707820240172172	25.69154558304787	27.848892737529766	24.751741439250193
27	20.478943548079005	26.586269690679636	29.89404918053663	23.040737580704725
28	19.365059151629378	23.86071032893145	31.01086250588969	25.76336801354948
29	22.870859704305527	21.952322131241484	29.992614005373948	25.184204159079044
30	22.98636137888879	22.89620130655698	30.373247418085498	23.744189896468733
31	21.972187909890867	27.748035707463675	25.446406968303897	24.833369414341565
32	23.78226597221338	27.90441504196009	25.094935499891758	23.218383485934773
33	22.771403466323683	27.532568415958842	26.610592535051637	23.085435582665834
34	21.72106409260509	24.80344340163256	28.219465916181697	25.256026589580653
35	23.407363072573766	23.555337654564674	26.764424974849415	26.272874298012148
36	24.576769773453716	24.096552778025394	28.57068269512397	22.755994753396923
37	20.854737860863143	26.578374317113855	29.599628153373995	22.967259668649
38	22.356769009385307	26.077272785156698	26.571625046162467	24.99433315929553
39	24.528378774179583	22.921670253543365	29.45305436346734	23.096896608809708
40	23.108612324423447	24.390337081513362	30.405465636023276	22.09558495803991
41	20.170259910603995	27.191411871076188	27.026500439339333	25.611827778980476
42	24.503673895602788	24.319024029951482	26.606390158798888	24.57091191564685
43	25.417754402944208	21.555388592458645	28.219465916181697	24.80739108841545
44	22.837368039018425	21.24301195767061	28.335731659174552	27.583888344136408
45	23.078176932774713	20.46939269295911	28.260088886624985	28.192341487641194
46	23.004571675984057	22.781845734588106	30.004075031517825	24.20950755791002
47	19.566645867026626	24.329975677155627	32.16855349115591	23.934824964661836
48	21.04562761852611	21.788556802119015	30.98564824837317	26.180167330981703
49	22.81571943408	20.242719064780268	32.79496224228609	24.146599258853644
50	22.2634253186802	20.15523323188203	29.846422249672088	27.734919199765685
51	20.227819730793232	20.73299629426821	27.57981331261859	31.45937066231997
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	138.0
1	449.5
2	761.0
3	1461.0
4	2161.0
5	1574.0
6	987.0
7	1051.0
8	1115.0
9	1204.5
10	1294.0
11	1359.0
12	1424.0
13	1329.5
14	1235.0
15	1285.5
16	1336.0
17	1434.5
18	1533.0
19	1809.0
20	2085.0
21	1891.5
22	1698.0
23	2130.0
24	2562.0
25	2879.0
26	4189.5
27	5183.0
28	6491.0
29	7799.0
30	8635.5
31	9472.0
32	11058.5
33	12645.0
34	14071.5
35	15498.0
36	17451.5
37	19405.0
38	21528.0
39	23651.0
40	27960.0
41	32269.0
42	38656.5
43	45044.0
44	52389.5
45	59735.0
46	73016.5
47	86298.0
48	97475.5
49	108653.0
50	102204.5
51	95756.0
52	78910.0
53	62064.0
54	52448.5
55	42833.0
56	38231.5
57	33630.0
58	31763.5
59	29897.0
60	26766.0
61	23635.0
62	20507.0
63	17379.0
64	14457.0
65	11535.0
66	9616.5
67	7698.0
68	6210.0
69	4722.0
70	3990.0
71	3258.0
72	2840.5
73	2423.0
74	1989.5
75	1240.0
76	924.0
77	685.0
78	446.0
79	322.5
80	199.0
81	143.0
82	87.0
83	52.0
84	17.0
85	12.0
86	7.0
87	15.5
88	24.0
89	13.5
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	785270.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.857333777331824
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.53487351934973	28.11331996802606
2	12.383827574504675	9.871726981559167
3	4.894662039631343	5.852645359224666
4	2.6098975704477256	4.160942343599296
5	1.703556504204095	3.39496100983036
6	1.2045142992246822	2.880523707826026
7	0.9364463616960996	2.612697864188261
8	0.7578691650750132	2.416531541755407
9	0.589643431999706	2.115145357094137
>10	4.232683232379884	28.837293987604195
>50	0.0967131717438951	2.592677148543221
>100	0.0485262426581572	3.337923888797658
>500	0.004072132251033173	1.11137892922105
>1k	0.0027147548340221155	2.7022319127306025
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	4747	0.6045054567218919	No Hit
CTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT	4304	0.548091739147045	No Hit
TCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	2967	0.37783182854304886	No Hit
GCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	2428	0.30919301641473634	No Hit
CTGTCACTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT	1717	0.2186509098781311	No Hit
CCTGTATCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	1697	0.21610401517949243	No Hit
TCTGTATCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	1085	0.13816903740114866	No Hit
CGCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG	1034	0.13167445591962001	No Hit
TCCTGTCTCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTG	971	0.12365173761890816	No Hit
GCTGTATCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGC	855	0.10887974836680378	No Hit
CTGTATCTTATACACATCTGACGCGTCGTAAGTCGTATGCCGTCTTCTGCT	803	0.10225782215034321	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5855310912170336	0.0
2	0.0	0.0	0.0	2.0051701962382364	0.0
3	0.0	0.0	0.0	2.7332000458441046	0.0
4	0.0	0.0	0.0	3.5029989685076472	0.0
5	0.0	0.0	0.0	4.707298126758949	0.0
6	0.0	0.0	0.0	5.614884052618844	0.0
7	0.0	0.0	0.0	6.790275956040597	0.0
8	0.0	0.0	0.0	8.602391534122022	0.0
9	0.0	0.0	0.0	9.466934939574923	0.0
10	0.0	0.0	0.0	11.050594063188457	0.0
11	0.0	0.0	0.0	13.942083614552956	0.0
12	0.0	0.0	0.0	16.682669655023112	0.0
13	0.0	0.0	0.0	17.558674086619888	0.0
14	0.0	0.0	0.0	17.873215581901768	0.0
15	0.0	0.0	0.0	18.30542361226075	0.0
16	0.0	0.0	0.0	19.36378570428006	0.0
17	0.0	0.0	0.0	20.90007258649891	0.0
18	0.0	0.0	0.0	22.606874068791626	0.0
19	0.0	0.0	0.0	23.65810485565474	0.0
20	0.0	0.0	0.0	24.812866912017522	0.0
21	0.0	0.0	0.0	26.27567588218065	0.0
22	0.0	0.0	0.0	27.797445464617265	0.0
23	0.0	0.0	0.0	29.1685662256294	0.0
24	0.0	0.0	0.0	30.25277929883989	0.0
25	0.0	0.0	0.0	31.158582398410736	0.0
26	0.0	0.0	0.0	31.92723521845989	0.0
27	0.0	0.0	0.0	32.69232238593095	0.0
28	0.0	0.0	0.0	33.45358921135406	0.0
29	0.0	0.0	0.0	34.331121779770015	0.0
30	0.0	0.0	0.0	35.21616768754696	0.0
31	0.0	0.0	0.0	36.090516637589616	0.0
32	0.0	0.0	0.0	36.94716466947674	0.0
33	0.0	0.0	0.0	37.70677601334573	0.0
34	1.2734473493193423E-4	0.0	0.0	38.48561641218944	0.0
35	1.2734473493193423E-4	0.0	0.0	39.28495931335719	0.0
36	1.2734473493193423E-4	0.0	0.0	40.093980414379764	0.0
37	1.2734473493193423E-4	0.0	0.0	40.910260165293465	0.0
38	1.2734473493193423E-4	0.0	0.0	41.68426146420976	0.0
39	1.2734473493193423E-4	0.0	0.0	42.48131216014874	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAACGAA	20	7.0319197E-4	45.0	10
TGACGTG	25	3.8896735E-5	45.0	20
TGCGCGA	25	3.8896735E-5	45.0	12
CGAGGTC	25	3.8896735E-5	45.0	16
TCCACGA	20	7.0319197E-4	45.0	31
TATTACG	20	7.0319197E-4	45.0	1
AACGAAC	20	7.0319197E-4	45.0	20
TTAGTAG	40	6.8102963E-9	45.0	1
GCGGAAT	20	7.0319197E-4	45.0	5
TCGACAA	25	3.8896735E-5	45.0	19
GTTATCC	25	3.8896735E-5	45.0	26
TGCGTTA	20	7.0319197E-4	45.0	34
CGCTTCA	20	7.0319197E-4	45.0	24
GTACGCT	25	3.8896735E-5	45.0	32
TTTCGCG	120	0.0	43.124996	1
ACGGGCG	95	0.0	42.63158	5
CGTTAGG	180	0.0	42.5	2
TTATGGG	2035	0.0	41.793613	3
TTGTTAG	350	0.0	41.785713	1
TTGCGAG	65	0.0	41.53846	1
>>END_MODULE