FastQCFastQC Report
Sat 14 Jan 2017
SRR2934104.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934104.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1446283
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC88110.6092168683445771Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT83300.5759592002395105Illumina PCR Primer Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC77390.5350958284097925Illumina PCR Primer Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC58750.4062137216575179Illumina PCR Primer Index 10 (95% over 23bp)
TCTGTATCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC32800.2267882565168781Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCACTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT30880.21351284637930473Illumina PCR Primer Index 10 (95% over 24bp)
CCTGTATCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC29840.20632199922145253Illumina PCR Primer Index 10 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG29140.20148200594212887Illumina PCR Primer Index 10 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC25110.17361747320545148Illumina PCR Primer Index 10 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG23840.16483634254153579Illumina PCR Primer Index 10 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC21290.14720493845257118No Hit
TTCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCT19830.1371100953271248Illumina PCR Primer Index 10 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG15840.10952213363498015Illumina PCR Primer Index 10 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC15460.10689470871191875No Hit
TGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG15440.10675642318965237Illumina PCR Primer Index 10 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACGAA207.033891E-445.00000435
TCGTTAG1600.045.0000041
CAACGAA302.1658925E-645.00000418
ACGCAAT207.033891E-445.00000426
GACCGAC207.033891E-445.0000049
TCGATCC207.033891E-445.00000417
TCGTCAG207.033891E-445.0000041
TCCGTGA207.033891E-445.00000417
ACCAACG207.033891E-445.00000421
ATCATCG207.033891E-445.00000424
CCAACGA253.891308E-545.022
CGTTATT4100.043.9024431
TGACGTA1300.043.2692312
CGTTTAT5050.043.217821
AAGCTCG1000.042.7533
CGTAATA850.042.3529416
TAGGGCG6100.042.0491835
CGTAGAT1400.041.78571315
TTCGTAG2550.041.470591
TAGGGTG8100.041.388895
Produced by FastQC (version 0.11.5)