##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934104.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1446283
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74180502709359	31.0	31.0	34.0	30.0	34.0
2	31.95997671271805	33.0	31.0	34.0	30.0	34.0
3	32.03947221947572	33.0	31.0	34.0	30.0	34.0
4	35.55932483476609	37.0	35.0	37.0	33.0	37.0
5	29.35908601566913	35.0	35.0	37.0	0.0	37.0
6	23.503505192275647	35.0	10.0	37.0	0.0	37.0
7	30.274333584782507	35.0	19.0	37.0	17.0	37.0
8	34.22300545605528	35.0	32.0	37.0	32.0	37.0
9	36.79699201331966	37.0	37.0	39.0	34.0	39.0
10	36.69893236662534	38.0	35.0	39.0	33.0	39.0
11	36.904539429696676	39.0	37.0	39.0	33.0	39.0
12	36.988096382243306	39.0	37.0	39.0	33.0	39.0
13	36.917894354009555	39.0	37.0	39.0	33.0	39.0
14	37.81946341068795	40.0	37.0	41.0	33.0	41.0
15	37.70544423186887	40.0	37.0	41.0	33.0	41.0
16	37.98065869542821	40.0	37.0	41.0	33.0	41.0
17	38.00576996341656	40.0	37.0	41.0	33.0	41.0
18	38.08788667224879	40.0	37.0	41.0	33.0	41.0
19	38.092216391950956	40.0	37.0	41.0	34.0	41.0
20	38.03330399375503	40.0	37.0	41.0	33.0	41.0
21	37.91858716447611	39.0	37.0	41.0	33.0	41.0
22	38.02528758202924	40.0	37.0	41.0	34.0	41.0
23	37.99966742331895	39.0	37.0	41.0	34.0	41.0
24	37.96824549552197	40.0	37.0	41.0	34.0	41.0
25	37.743488653327184	39.0	36.0	41.0	33.0	41.0
26	37.63861844466125	39.0	36.0	41.0	33.0	41.0
27	36.82474384335569	39.0	36.0	40.0	32.0	41.0
28	37.19729402890029	39.0	36.0	40.0	32.0	41.0
29	37.519641729868916	39.0	36.0	41.0	32.0	41.0
30	37.501529092162464	39.0	36.0	40.0	33.0	41.0
31	37.44896676514901	39.0	36.0	40.0	33.0	41.0
32	37.52095336804761	39.0	36.0	40.0	33.0	41.0
33	36.38748640480459	39.0	36.0	40.0	31.0	41.0
34	36.85099112690946	39.0	36.0	40.0	31.0	41.0
35	37.212911995785056	39.0	36.0	40.0	31.0	41.0
36	37.33776031385282	39.0	36.0	40.0	32.0	41.0
37	37.372834362292856	39.0	36.0	40.0	32.0	41.0
38	37.33625369308773	39.0	36.0	40.0	33.0	41.0
39	37.39307106562132	39.0	36.0	40.0	33.0	41.0
40	37.30599682081584	39.0	36.0	40.0	32.0	41.0
41	37.21521030116512	39.0	36.0	40.0	32.0	41.0
42	37.305883426687586	39.0	36.0	40.0	33.0	41.0
43	37.20523714929927	39.0	35.0	40.0	32.0	41.0
44	37.23815740073001	39.0	35.0	40.0	32.0	41.0
45	37.17817052402607	39.0	35.0	40.0	32.0	41.0
46	37.08916443047453	39.0	35.0	40.0	32.0	41.0
47	37.01888980234159	39.0	35.0	40.0	32.0	41.0
48	37.01032163138196	39.0	35.0	40.0	32.0	41.0
49	37.087838272316	39.0	35.0	40.0	32.0	41.0
50	37.00484137613454	39.0	35.0	40.0	32.0	41.0
51	36.17311342247679	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	5.0
11	5.0
12	10.0
13	7.0
14	19.0
15	21.0
16	29.0
17	82.0
18	164.0
19	297.0
20	493.0
21	916.0
22	1473.0
23	2473.0
24	3928.0
25	5761.0
26	7589.0
27	9711.0
28	12780.0
29	17192.0
30	24209.0
31	34671.0
32	50746.0
33	76856.0
34	104216.0
35	129124.0
36	183274.0
37	274015.0
38	292388.0
39	213768.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.501209652606025	4.220474139570195	58.574497522269155	13.703818685554625
2	20.029551616108325	4.065801782915238	52.627874351008764	23.276772249967674
3	19.00188275738566	3.9457008068268795	53.687418022613834	23.36499841317363
4	18.785051058471957	4.034410969360768	51.71989161180765	25.460646360359622
5	14.311514413154272	21.8041697233529	43.31213185801119	20.57218400548164
6	14.872193063183348	29.125696699746868	41.342185450565346	14.659924786504439
7	78.25840447547264	1.4995682034567233	13.564012022543306	6.678015298527329
8	79.11833299568617	1.7049222040223109	10.694864006560264	8.481880793731241
9	74.02347949882561	4.12360513122259	14.265050477672766	7.587864892279035
10	35.79935600432281	21.222264245655932	24.85910433850083	18.119275411520427
11	25.91698858383871	25.43119154411688	26.321197165423367	22.330622706621043
12	21.979861479392344	21.413789693994882	34.30511179347334	22.301237033139433
13	22.744165560958677	24.413548385758528	35.37675544827672	17.465530605006073
14	19.588766513884213	26.200266476201406	33.83798329925747	20.372983710656907
15	17.02868664016655	26.81052048596298	35.5189821079277	20.641810765942765
16	18.99068163008208	25.183038174409848	31.47039687253463	24.355883322973444
17	19.23565443277699	26.819439902149167	28.157144901793078	25.78776076328077
18	20.440121331717236	24.101852818570084	33.02334328758618	22.434682562126497
19	20.604335389408572	27.808319671876113	29.440918547753103	22.146426390962212
20	20.23352276145125	28.755298928356343	30.816513780498006	20.194664529694396
21	20.297963814827387	28.318800677322486	31.621058949043864	19.762176558806264
22	18.167260487746866	25.707970016933064	30.39066351467866	25.73410598064141
23	17.995993868419944	26.50442548242633	28.777217183635567	26.72236346551816
24	20.53858062357091	25.898734894899544	28.576080891499107	24.98660359003044
25	18.779727065864705	26.51631803734124	25.83007613309428	28.87387876369977
26	16.79733496141488	26.34242399309126	28.109367253850042	28.750873791643823
27	17.691558291150486	27.12754004575868	28.336017224844657	26.84488443824618
28	14.966503789369023	26.07802207451792	29.64703311869116	29.3084410174219
29	15.94840014022152	23.804124089130553	31.313166233717745	28.934309536930186
30	19.128828866826204	25.110023418653192	30.19713292626685	25.564014788253754
31	17.396456986634014	30.758295575623855	27.247917592891568	24.597329844850556
32	18.74978825029403	27.880504714499168	26.858782133233948	26.51092490197285
33	18.293376884053814	27.319618636186693	26.72858631402015	27.658418165739345
34	16.032546880520616	26.85228271368743	29.08365790097789	28.031512504814067
35	16.56466957020168	23.98721412061125	27.387585970380623	32.060530338806444
36	18.740177406496517	25.641523823484064	26.316080601099507	29.302218168919914
37	15.953101847978576	29.60471774887764	29.09174760403047	25.350432799113314
38	17.735118230664398	28.182589437890094	30.154126128842	23.928166202603503
39	17.69708971204114	23.373779543837546	33.46281467734876	25.46631606677255
40	20.95143205029721	24.46291631720763	30.544160444394354	24.041491188100807
41	19.54237172116384	24.708303976469338	25.048278932961253	30.701045369405573
42	20.234075903540315	22.75847811251325	25.904266315790203	31.103179668156233
43	19.404362769941983	22.07451791938369	29.168496068888317	29.352623241786013
44	17.62794695090795	22.26673479533397	29.419138577996147	30.686179675761938
45	18.97035365830892	20.44406246910183	28.890403883610606	31.695179988978644
46	19.89257980630347	21.63967909461703	30.778623547397014	27.689117551682486
47	15.524900728280702	24.578661299344596	32.37063562248882	27.52580234988588
48	16.057092560722904	23.303945355093024	31.343796476899747	29.29516560728433
49	19.58807508627288	20.17544284209937	33.41890902402918	26.817573047598568
50	19.04931469152303	19.637304732199716	30.870721705226433	30.442658871050828
51	17.73560222999233	19.762660558134197	27.507272089902184	34.99446512197129
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	154.0
1	491.5
2	829.0
3	2371.0
4	3913.0
5	2814.0
6	1715.0
7	1764.0
8	1813.0
9	2031.0
10	2249.0
11	2370.0
12	2491.0
13	2521.5
14	2552.0
15	2648.5
16	2745.0
17	2930.5
18	3116.0
19	3292.5
20	3469.0
21	3673.0
22	3877.0
23	4542.0
24	5207.0
25	6337.0
26	7868.5
27	8270.0
28	10188.5
29	12107.0
30	14337.0
31	16567.0
32	19630.0
33	22693.0
34	27407.5
35	32122.0
36	35788.0
37	39454.0
38	44611.5
39	49769.0
40	58310.5
41	66852.0
42	80134.5
43	93417.0
44	113103.5
45	132790.0
46	164320.5
47	195851.0
48	218826.5
49	241802.0
50	217431.0
51	193060.0
52	151687.5
53	110315.0
54	88055.0
55	65795.0
56	53566.5
57	41338.0
58	34401.5
59	27465.0
60	22525.0
61	17585.0
62	14908.0
63	12231.0
64	10697.0
65	9163.0
66	7521.5
67	5880.0
68	4753.5
69	3627.0
70	3068.5
71	2510.0
72	2125.0
73	1740.0
74	1466.0
75	902.5
76	613.0
77	461.0
78	309.0
79	194.0
80	79.0
81	63.5
82	48.0
83	40.0
84	32.0
85	19.5
86	7.0
87	4.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1446283.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.505554940808274
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.38635356589724	32.21594835591813
2	13.150459249686211	11.7053697326754
3	5.06015053385537	6.756144227797814
4	2.5431042926307663	4.527290712635358
5	1.5507663329601509	3.450885811595689
6	1.037661090226713	2.7709009596614385
7	0.7426794236358699	2.3137351924423823
8	0.5702595473348425	2.0303774091545033
9	0.45713326033844587	1.8310472489937164
>10	2.3142083347154196	17.03881780759113
>50	0.11401166580417192	3.505609811099871
>100	0.06452934495803711	5.473759791684421
>500	0.00527811954234639	1.632897297019234
>1k	0.002724190731533621	2.416724372074548
>5k	6.810476828834053E-4	2.330491269656436
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	8811	0.6092168683445771	Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	8330	0.5759592002395105	Illumina PCR Primer Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	7739	0.5350958284097925	Illumina PCR Primer Index 10 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	5875	0.4062137216575179	Illumina PCR Primer Index 10 (95% over 23bp)
TCTGTATCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	3280	0.2267882565168781	Illumina PCR Primer Index 10 (95% over 23bp)
CTGTCACTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGCT	3088	0.21351284637930473	Illumina PCR Primer Index 10 (95% over 24bp)
CCTGTATCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	2984	0.20632199922145253	Illumina PCR Primer Index 10 (95% over 23bp)
TCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	2914	0.20148200594212887	Illumina PCR Primer Index 10 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTGC	2511	0.17361747320545148	Illumina PCR Primer Index 10 (95% over 23bp)
CGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	2384	0.16483634254153579	Illumina PCR Primer Index 10 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCC	2129	0.14720493845257118	No Hit
TTCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCT	1983	0.1371100953271248	Illumina PCR Primer Index 10 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	1584	0.10952213363498015	Illumina PCR Primer Index 10 (95% over 22bp)
CGTTCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTC	1546	0.10689470871191875	No Hit
TGCTGTCTCTTATACACATCTGACGCCCATTAGCTCGTATGCCGTCTTCTG	1544	0.10675642318965237	Illumina PCR Primer Index 10 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6191042831866239	0.0
2	0.0	0.0	0.0	2.324026487208935	0.0
3	0.0	0.0	0.0	3.267272034587975	0.0
4	0.0	0.0	0.0	4.28768090339166	0.0
5	0.0	0.0	0.0	5.926433485009504	0.0
6	0.0	6.914276113319454E-5	0.0	7.333834387875679	0.0
7	0.0	6.914276113319454E-5	0.0	8.907454488506053	0.0
8	0.0	6.914276113319454E-5	0.0	11.438148688742107	0.0
9	0.0	6.914276113319454E-5	0.0	12.44901585650941	0.0
10	0.0	6.914276113319454E-5	0.0	14.508571282383876	0.0
11	0.0	6.914276113319454E-5	0.0	18.46823892695966	0.0
12	0.0	6.914276113319454E-5	0.0	21.524694682852527	0.0
13	0.0	6.914276113319454E-5	0.0	22.64121198963135	0.0
14	0.0	6.914276113319454E-5	0.0	23.001238346851895	0.0
15	0.0	6.914276113319454E-5	0.0	23.520638768484453	0.0
16	0.0	6.914276113319454E-5	0.0	24.881437450346855	0.0
17	0.0	6.914276113319454E-5	0.0	26.854771853088227	0.0
18	0.0	6.914276113319454E-5	0.0	29.032768828783855	0.0
19	0.0	6.914276113319454E-5	0.0	30.425788037334325	0.0
20	6.914276113319454E-5	6.914276113319454E-5	0.0	31.781539297634005	0.0
21	6.914276113319454E-5	6.914276113319454E-5	0.0	33.80230563451275	0.0
22	6.914276113319454E-5	6.914276113319454E-5	0.0	35.801845143723604	0.0
23	6.914276113319454E-5	6.914276113319454E-5	0.0	37.87232512585711	0.0
24	6.914276113319454E-5	6.914276113319454E-5	0.0	39.303511138553105	0.0
25	6.914276113319454E-5	6.914276113319454E-5	0.0	40.599384767711435	0.0
26	1.3828552226638908E-4	6.914276113319454E-5	0.0	41.71811464284652	0.0
27	1.3828552226638908E-4	6.914276113319454E-5	0.0	42.89492443733349	0.0
28	1.3828552226638908E-4	6.914276113319454E-5	0.0	44.066133668168675	0.0
29	1.3828552226638908E-4	6.914276113319454E-5	0.0	45.30295937931926	0.0
30	1.3828552226638908E-4	6.914276113319454E-5	0.0	46.561150203660006	0.0
31	1.3828552226638908E-4	6.914276113319454E-5	0.0	47.73816742643037	0.0
32	1.3828552226638908E-4	6.914276113319454E-5	0.0	48.800061951913975	0.0
33	1.3828552226638908E-4	6.914276113319454E-5	0.0	49.84646849890374	0.0
34	1.3828552226638908E-4	6.914276113319454E-5	0.0	50.86563279800703	0.0
35	1.3828552226638908E-4	6.914276113319454E-5	0.0	51.913698771263995	0.0
36	1.3828552226638908E-4	6.914276113319454E-5	0.0	52.93217164275595	0.0
37	1.3828552226638908E-4	6.914276113319454E-5	0.0	53.94939994454751	0.0
38	1.3828552226638908E-4	6.914276113319454E-5	0.0	55.00175276899473	0.0
39	1.3828552226638908E-4	6.914276113319454E-5	0.0	56.386543988970345	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAA	20	7.033891E-4	45.000004	35
TCGTTAG	160	0.0	45.000004	1
CAACGAA	30	2.1658925E-6	45.000004	18
ACGCAAT	20	7.033891E-4	45.000004	26
GACCGAC	20	7.033891E-4	45.000004	9
TCGATCC	20	7.033891E-4	45.000004	17
TCGTCAG	20	7.033891E-4	45.000004	1
TCCGTGA	20	7.033891E-4	45.000004	17
ACCAACG	20	7.033891E-4	45.000004	21
ATCATCG	20	7.033891E-4	45.000004	24
CCAACGA	25	3.891308E-5	45.0	22
CGTTATT	410	0.0	43.902443	1
TGACGTA	130	0.0	43.26923	12
CGTTTAT	505	0.0	43.21782	1
AAGCTCG	100	0.0	42.75	33
CGTAATA	85	0.0	42.35294	16
TAGGGCG	610	0.0	42.049183	5
CGTAGAT	140	0.0	41.785713	15
TTCGTAG	255	0.0	41.47059	1
TAGGGTG	810	0.0	41.38889	5
>>END_MODULE