FastQCFastQC Report
Sat 14 Jan 2017
SRR2934101.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934101.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1758582
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC87770.4990952938219543No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC69870.39730874079229744No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG68850.3915086131894902No Hit
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC54430.30951073080470515TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT53360.30342628322136816TruSeq Adapter, Index 19 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC47140.268056877643465TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC37810.21500276927661038TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTATCTGT26890.1529072855289091No Hit
GAATCAGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC26450.15040526970024712No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTC22300.1268067113162764No Hit
GAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT19670.11185148034041062No Hit
CTGTCACTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT19180.1090651445312189TruSeq Adapter, Index 19 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT19160.10895141653900699No Hit
CCTGTATCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC19070.1084396405740534TruSeq Adapter, Index 19 (95% over 21bp)
GCTGTATCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC18560.10553957677264979TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18350.10434543285442477No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAG253.8916514E-545.00000442
TCGTTAG2000.043.8750041
CCTACGG1200.041.2500042
TAGGGCG8200.041.1585355
TTAGGGA39700.041.032754
TCGACGT556.184564E-1140.90909226
CCGATGA7750.040.6451618
TTACGGG15250.040.5737723
ACGCTAA501.0822987E-940.50000427
AATACGG2500.040.4999965
TTTACGG9800.040.4081652
TAGGGAT23500.040.4042555
TATGGGA31750.040.039374
CTACGGG7100.039.9295773
TTCCGAA1750.039.85714316
AGGGCGA11600.039.7629286
CGTTAGG5050.039.6534652
TTCGTAG3650.039.4520531
TACGGGT2400.039.3750044
TAGGGAG20500.039.2926865
Produced by FastQC (version 0.11.5)