##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934101.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1758582
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.86823759142309	33.0	31.0	34.0	30.0	34.0
2	32.049681504757814	33.0	31.0	34.0	30.0	34.0
3	32.128999387006125	34.0	31.0	34.0	30.0	34.0
4	35.62018660488962	37.0	35.0	37.0	33.0	37.0
5	29.424873562904658	37.0	35.0	37.0	0.0	37.0
6	23.647157198242674	35.0	10.0	37.0	0.0	37.0
7	30.343686561104345	35.0	19.0	37.0	17.0	37.0
8	34.115062021560554	35.0	32.0	37.0	32.0	37.0
9	36.789813611193566	37.0	37.0	39.0	34.0	39.0
10	36.89189073924332	39.0	37.0	39.0	33.0	39.0
11	37.068901535441626	39.0	37.0	39.0	34.0	39.0
12	37.064211393042804	39.0	37.0	39.0	34.0	39.0
13	36.98992711172979	39.0	37.0	39.0	33.0	39.0
14	37.92099941884996	40.0	37.0	41.0	33.0	41.0
15	37.816601102479154	40.0	37.0	41.0	33.0	41.0
16	38.111225976383245	40.0	37.0	41.0	33.0	41.0
17	38.108291794184176	40.0	37.0	41.0	33.0	41.0
18	38.199077438527176	40.0	37.0	41.0	34.0	41.0
19	38.214958415359646	40.0	37.0	41.0	34.0	41.0
20	38.115649995280286	40.0	37.0	41.0	33.0	41.0
21	38.034191183578585	40.0	37.0	41.0	33.0	41.0
22	38.15802333925856	40.0	37.0	41.0	34.0	41.0
23	38.08516008920824	40.0	37.0	41.0	34.0	41.0
24	38.057526461660586	40.0	37.0	41.0	34.0	41.0
25	37.8286784466121	40.0	36.0	41.0	33.0	41.0
26	37.7456848756555	40.0	36.0	41.0	33.0	41.0
27	36.90046014345649	40.0	36.0	41.0	32.0	41.0
28	37.264783217387645	40.0	36.0	41.0	32.0	41.0
29	37.535752100271694	40.0	36.0	41.0	32.0	41.0
30	37.56699659157207	40.0	36.0	41.0	33.0	41.0
31	37.59814555135899	40.0	36.0	41.0	33.0	41.0
32	37.58345928708471	40.0	36.0	41.0	33.0	41.0
33	36.42478087459101	39.0	35.0	41.0	31.0	41.0
34	36.85814878123397	40.0	35.0	41.0	31.0	41.0
35	37.14553828027354	40.0	35.0	41.0	31.0	41.0
36	37.30544722964297	39.0	35.0	41.0	32.0	41.0
37	37.38658760296648	39.0	36.0	41.0	33.0	41.0
38	37.307402782469055	39.0	35.0	41.0	33.0	41.0
39	37.321364599432954	39.0	35.0	41.0	33.0	41.0
40	37.26301304118887	39.0	35.0	41.0	33.0	41.0
41	37.181393873018145	39.0	35.0	41.0	32.0	41.0
42	37.21958145824306	39.0	35.0	41.0	33.0	41.0
43	37.0916238196456	39.0	35.0	41.0	32.0	41.0
44	37.107944923807935	39.0	35.0	41.0	32.0	41.0
45	37.06306672080119	39.0	35.0	41.0	32.0	41.0
46	37.014154585910696	39.0	35.0	41.0	32.0	41.0
47	36.936136045973406	39.0	35.0	40.0	32.0	41.0
48	36.88161996426666	39.0	35.0	40.0	32.0	41.0
49	36.91629164861235	39.0	35.0	40.0	32.0	41.0
50	36.8106878155241	39.0	35.0	40.0	32.0	41.0
51	36.04877736721973	38.0	35.0	40.0	30.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	6.0
10	16.0
11	19.0
12	20.0
13	22.0
14	36.0
15	50.0
16	89.0
17	128.0
18	268.0
19	517.0
20	985.0
21	1749.0
22	2972.0
23	4395.0
24	6020.0
25	7959.0
26	9985.0
27	12238.0
28	15417.0
29	20732.0
30	29131.0
31	40968.0
32	58997.0
33	92748.0
34	125825.0
35	151742.0
36	207117.0
37	308989.0
38	355576.0
39	303709.0
40	154.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.097787876823485	3.916109683824809	53.01703304139358	16.96906939795813
2	19.80561611571141	7.1155055607301785	51.49205439382412	21.586823929734297
3	20.845146828524346	7.393627365684398	49.83560618725769	21.925619618533567
4	18.770691386583056	4.792554455805871	51.57826021192074	24.858493945690334
5	16.572670481103525	22.439215231362542	39.707104928857454	21.281009358676478
6	16.262420518349444	31.094597806641943	38.844762427910666	13.798219247097945
7	80.27518762275515	2.1625946359055193	12.151551647861742	5.4106660934775865
8	81.02857870716294	4.2665056278296944	8.631556560911006	6.073359104096369
9	75.55524848997658	5.119920481387846	10.898951541639798	8.425879486995774
10	38.8946890164917	28.088994428465664	17.355858299470825	15.660458255571818
11	29.67402145592301	22.922274878282618	27.08335465733187	20.3203490084625
12	25.486784238665017	20.13485865316488	31.77662457593675	22.601732532233356
13	25.755239164281225	20.51510819512539	34.04862554035012	19.681027100243263
14	20.859021643574195	23.897833595476357	33.46082241260288	21.782322348346568
15	18.581846055515182	22.717962540273927	36.44720576009534	22.252985644115544
16	22.242863852808682	23.001998200823163	30.533975669033346	24.221162277334805
17	22.234846029357744	23.374400511321053	30.418086844969412	23.97266661435179
18	22.814176421685197	21.565499931194566	31.577145677597063	24.04317796952317
19	23.465951545051638	23.721668935540112	29.383560163813797	23.428819355594452
20	24.03254440225136	26.78100879003652	29.558928727804563	19.627518079907563
21	23.973747030277803	25.937260815816384	30.331084930927304	19.757907222978513
22	21.554127131973374	22.204878703409907	31.48053374821305	24.76046041640367
23	21.20327627599964	24.51219220940508	29.98796757842398	24.2965639361713
24	22.325202919170103	24.798104381825812	28.169627574943906	24.70706512406018
25	22.335267846480857	26.305000278633585	26.026821609683253	25.332910265202308
26	21.33366541907059	24.093843790053576	28.083592348835595	26.488898442040238
27	20.811369614837407	25.321025690016164	29.29712688973275	24.570477805413677
28	18.951007118235033	24.058360656483462	30.98831899791992	26.00231322736159
29	22.162060114342125	23.778191747669432	29.104755990906312	24.954992147082137
30	22.94894409245631	22.984711546006952	30.25949315982991	23.80685120170683
31	22.547939191917123	23.636259213388968	28.893108197399954	24.92269339729396
32	23.945030712244296	25.467393615992883	27.552141441229356	23.035434230533465
33	23.293426180866174	24.384589402143316	26.943696682895652	25.378287734094858
34	21.699300914031873	22.419654016702093	30.68517703467908	25.195868034586955
35	21.675929811632326	23.691985929572805	28.469243970426174	26.1628402883687
36	24.533004431979858	23.292459492932373	28.291942030567807	23.88259404451996
37	20.352022254293516	24.80009462168952	30.01639957647696	24.831483547540007
38	21.19457608459543	24.698421796652077	30.44703061898734	23.65997149976515
39	22.197315791927817	23.13386580779287	29.419782529333293	25.249035870946024
40	22.400547714010493	20.96660832420666	32.10643575335128	24.526408208431565
41	21.573347162657186	21.669788500052885	28.52605110253602	28.23081323475391
42	22.562382646928036	21.205152787871135	31.194052935831255	25.038411629369573
43	23.968856726612692	21.093016987550197	29.87077088244961	25.067355403387502
44	21.718350352727366	21.62810719090722	30.022938936029142	26.630603520336273
45	21.56146258747104	20.07361612935877	28.68566833960543	29.67925294356476
46	22.787336615523188	21.450634659060537	30.289119301801108	25.472909423615164
47	18.452082416401396	22.788075847472566	34.2145546809873	24.545287055138743
48	19.7988493001748	23.574504913617904	30.802316866657343	25.824328919549956
49	22.343911173888962	20.450681287537346	33.19242435098278	24.012983187590912
50	21.59324956129427	20.593466781759396	30.228672873940482	27.584610783005854
51	20.13400569322329	20.36584020534726	28.543167165363915	30.956986936065533
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	265.0
1	933.5
2	1602.0
3	3721.5
4	5841.0
5	4085.0
6	2329.0
7	2312.0
8	2295.0
9	2416.5
10	2538.0
11	2594.5
12	2651.0
13	2742.5
14	2834.0
15	2759.0
16	2684.0
17	2768.5
18	2853.0
19	3010.5
20	3168.0
21	4012.0
22	4856.0
23	5195.0
24	5534.0
25	7226.5
26	10672.5
27	12426.0
28	14999.5
29	17573.0
30	21068.0
31	24563.0
32	28193.0
33	31823.0
34	36128.0
35	40433.0
36	43881.5
37	47330.0
38	53011.5
39	58693.0
40	66097.5
41	73502.0
42	83591.0
43	93680.0
44	109572.0
45	125464.0
46	163025.0
47	200586.0
48	206276.5
49	211967.0
50	201143.5
51	190320.0
52	163489.0
53	136658.0
54	121320.5
55	105983.0
56	95501.5
57	85020.0
58	77435.5
59	69851.0
60	60620.5
61	51390.0
62	46737.0
63	42084.0
64	36338.5
65	30593.0
66	25714.5
67	20836.0
68	17335.0
69	13834.0
70	11820.5
71	9807.0
72	8404.5
73	7002.0
74	5855.0
75	3387.0
76	2066.0
77	1548.5
78	1031.0
79	810.5
80	590.0
81	414.5
82	239.0
83	151.5
84	64.0
85	47.5
86	31.0
87	30.0
88	29.0
89	32.0
90	35.0
91	18.5
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	1758582.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.53843285589393
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.46735001110791	26.827723197806442
2	11.775307022479362	8.840531440417555
3	4.465257750484629	5.028563347524817
4	2.2928977070470093	3.442871464856692
5	1.409192824807466	2.6449445117521284
6	1.0648780561573181	2.3984312046465806
7	0.8709447881486162	2.288573171577531
8	0.7584081675852521	2.277556326100843
9	0.6396195937309179	2.1609285455323994
>10	5.105495889522173	32.881967381613606
>50	0.0940622954576358	2.431695087694669
>100	0.04862853394825921	3.179112565639756
>500	0.004067094867470809	0.9972824568865143
>1k	0.003006113597695815	2.3808923830940825
>5k	8.84151058145828E-4	2.21892691485639
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGC	8777	0.4990952938219543	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	6987	0.39730874079229744	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCG	6885	0.3915086131894902	No Hit
CCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	5443	0.30951073080470515	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	5336	0.30342628322136816	TruSeq Adapter, Index 19 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	4714	0.268056877643465	TruSeq Adapter, Index 13 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	3781	0.21500276927661038	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTATCTGT	2689	0.1529072855289091	No Hit
GAATCAGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTC	2645	0.15040526970024712	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTC	2230	0.1268067113162764	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCT	1967	0.11185148034041062	No Hit
CTGTCACTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGCT	1918	0.1090651445312189	TruSeq Adapter, Index 19 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCT	1916	0.10895141653900699	No Hit
CCTGTATCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	1907	0.1084396405740534	TruSeq Adapter, Index 19 (95% over 21bp)
GCTGTATCTTATACACATCTGACGCGCTATCTGTCGTATGCCGTCTTCTGC	1856	0.10553957677264979	TruSeq Adapter, Index 19 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1835	0.10434543285442477	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3215658979791673	0.0
2	0.0	0.0	0.0	1.1807808791401253	0.0
3	0.0	0.0	0.0	1.6117531056271472	0.0
4	0.0	0.0	0.0	2.2142840083658313	0.0
5	0.0	0.0	0.0	3.398362999280102	0.0
6	0.0	0.0	0.0	4.200657120339001	0.0
7	5.686399610595355E-5	0.0	0.0	5.242576120988387	0.0
8	1.137279922119071E-4	0.0	0.0	6.807188973843699	0.0
9	1.137279922119071E-4	0.0	0.0	7.517704605187588	0.0
10	1.137279922119071E-4	0.0	0.0	9.204347593686277	0.0
11	1.137279922119071E-4	0.0	0.0	11.371320757291954	0.0
12	1.137279922119071E-4	0.0	0.0	13.97074461128341	0.0
13	1.137279922119071E-4	0.0	0.0	14.685923090307988	0.0
14	1.137279922119071E-4	0.0	0.0	14.966831231071398	0.0
15	1.137279922119071E-4	0.0	0.0	15.400987841340353	0.0
16	1.137279922119071E-4	0.0	0.0	16.203054506414826	0.0
17	1.137279922119071E-4	0.0	0.0	17.249863810729327	0.0
18	1.137279922119071E-4	0.0	0.0	18.36587659830477	0.0
19	1.7059198831786065E-4	0.0	0.0	19.340582355556922	0.0
20	1.7059198831786065E-4	0.0	0.0	20.228968566720233	0.0
21	1.7059198831786065E-4	0.0	0.0	21.37938407193978	0.0
22	1.7059198831786065E-4	0.0	0.0	22.56949064644128	0.0
23	1.7059198831786065E-4	0.0	0.0	23.656957707971536	0.0
24	1.7059198831786065E-4	0.0	0.0	24.508894097630932	0.0
25	1.7059198831786065E-4	0.0	0.0	25.241416095467827	0.0
26	1.7059198831786065E-4	0.0	0.0	25.90183454624237	0.0
27	1.7059198831786065E-4	0.0	0.0	26.606208866006817	0.0
28	1.7059198831786065E-4	0.0	0.0	27.309616497837464	0.0
29	1.7059198831786065E-4	0.0	0.0	28.077280445267835	0.0
30	1.7059198831786065E-4	0.0	0.0	28.91784403570604	0.0
31	1.7059198831786065E-4	0.0	0.0	29.645248273893397	0.0
32	2.274559844238142E-4	0.0	0.0	30.348485313735726	0.0
33	2.274559844238142E-4	0.0	0.0	31.03619848264113	0.0
34	2.274559844238142E-4	0.0	0.0	31.80306633412602	0.0
35	2.274559844238142E-4	0.0	0.0	32.60405258327448	0.0
36	2.274559844238142E-4	0.0	0.0	33.33066072551635	0.0
37	2.274559844238142E-4	0.0	0.0	34.05647277181274	0.0
38	2.8431998052976773E-4	0.0	0.0	34.899197194102975	0.0
39	2.8431998052976773E-4	0.0	0.0	36.21099272027122	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGATAAG	25	3.8916514E-5	45.000004	42
TCGTTAG	200	0.0	43.875004	1
CCTACGG	120	0.0	41.250004	2
TAGGGCG	820	0.0	41.158535	5
TTAGGGA	3970	0.0	41.03275	4
TCGACGT	55	6.184564E-11	40.909092	26
CCGATGA	775	0.0	40.64516	18
TTACGGG	1525	0.0	40.573772	3
ACGCTAA	50	1.0822987E-9	40.500004	27
AATACGG	250	0.0	40.499996	5
TTTACGG	980	0.0	40.408165	2
TAGGGAT	2350	0.0	40.404255	5
TATGGGA	3175	0.0	40.03937	4
CTACGGG	710	0.0	39.929577	3
TTCCGAA	175	0.0	39.857143	16
AGGGCGA	1160	0.0	39.762928	6
CGTTAGG	505	0.0	39.653465	2
TTCGTAG	365	0.0	39.452053	1
TACGGGT	240	0.0	39.375004	4
TAGGGAG	2050	0.0	39.292686	5
>>END_MODULE