##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934100.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	752357
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.930414683454796	33.0	31.0	34.0	30.0	34.0
2	32.11177273554974	33.0	31.0	34.0	30.0	34.0
3	32.200888673860945	34.0	31.0	34.0	30.0	34.0
4	35.68314643181362	37.0	35.0	37.0	33.0	37.0
5	29.255155464759415	37.0	35.0	37.0	0.0	37.0
6	23.467934770328448	35.0	10.0	37.0	0.0	37.0
7	30.294696533693447	35.0	19.0	37.0	17.0	37.0
8	34.13447339494416	35.0	32.0	37.0	32.0	37.0
9	36.859700913263254	38.0	37.0	39.0	34.0	39.0
10	36.98656887621169	39.0	37.0	39.0	34.0	39.0
11	37.141210887916245	39.0	37.0	39.0	34.0	39.0
12	37.0556065803867	39.0	37.0	39.0	34.0	39.0
13	36.917472689162196	39.0	37.0	39.0	33.0	39.0
14	37.897657627961195	40.0	37.0	41.0	33.0	41.0
15	37.81027889685349	40.0	37.0	41.0	33.0	41.0
16	38.114049580186006	40.0	37.0	41.0	33.0	41.0
17	38.111714252675256	40.0	37.0	41.0	33.0	41.0
18	38.200657400675475	40.0	37.0	41.0	34.0	41.0
19	38.211738576234424	40.0	37.0	41.0	34.0	41.0
20	38.09733676964526	40.0	37.0	41.0	34.0	41.0
21	38.00865679458023	40.0	37.0	41.0	33.0	41.0
22	38.1244807983444	40.0	37.0	41.0	34.0	41.0
23	38.044037604488295	40.0	36.0	41.0	34.0	41.0
24	38.024169377037765	40.0	36.0	41.0	34.0	41.0
25	37.7989637897966	40.0	36.0	41.0	33.0	41.0
26	37.681473024109565	40.0	36.0	41.0	33.0	41.0
27	36.780992268298164	39.0	35.0	41.0	32.0	41.0
28	37.155967180474164	39.0	35.0	41.0	32.0	41.0
29	37.446819794326366	39.0	35.0	41.0	32.0	41.0
30	37.46396192233208	39.0	36.0	41.0	33.0	41.0
31	37.49730513572679	39.0	36.0	41.0	33.0	41.0
32	37.46889442113252	39.0	35.0	41.0	33.0	41.0
33	36.22901361986398	39.0	35.0	41.0	31.0	41.0
34	36.689733730130776	39.0	35.0	41.0	31.0	41.0
35	37.04481250257524	39.0	35.0	41.0	31.0	41.0
36	37.184113392977004	39.0	35.0	41.0	32.0	41.0
37	37.29662381023902	39.0	35.0	41.0	33.0	41.0
38	37.193796296173225	39.0	35.0	41.0	33.0	41.0
39	37.23866462331048	39.0	35.0	41.0	33.0	41.0
40	37.1469382221472	39.0	35.0	41.0	33.0	41.0
41	37.07536182955698	39.0	35.0	41.0	32.0	41.0
42	37.14850662650843	39.0	35.0	41.0	33.0	41.0
43	37.05502175164184	39.0	35.0	41.0	33.0	41.0
44	37.10279561431608	39.0	35.0	41.0	33.0	41.0
45	37.01033153144053	39.0	35.0	41.0	33.0	41.0
46	36.936822545679775	39.0	35.0	40.0	33.0	41.0
47	36.87786649157248	39.0	35.0	40.0	32.0	41.0
48	36.844828984112596	39.0	35.0	40.0	32.0	41.0
49	36.89700368309194	39.0	35.0	40.0	33.0	41.0
50	36.813127278672226	39.0	35.0	40.0	32.0	41.0
51	36.09190184978674	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	8.0
10	11.0
11	10.0
12	13.0
13	7.0
14	25.0
15	19.0
16	39.0
17	56.0
18	97.0
19	178.0
20	384.0
21	699.0
22	1240.0
23	1765.0
24	2488.0
25	3195.0
26	4197.0
27	5140.0
28	6703.0
29	8580.0
30	11987.0
31	17514.0
32	25519.0
33	41959.0
34	57052.0
35	71344.0
36	88447.0
37	130691.0
38	148199.0
39	124722.0
40	68.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.08753955901254	4.411469555011783	51.193648759830765	17.3073421261449
2	19.187699456508014	7.607558645696126	51.29812044016337	21.90662145763248
3	20.874664554194354	7.5017578091251895	49.56051448979673	22.063063146883728
4	19.448081163596537	4.616026700090516	51.455625454405286	24.480266681907658
5	17.111158665367636	22.538369417709944	39.66228798296553	20.688183933956886
6	15.949210281820998	31.3377824623151	38.89456733970708	13.818439916156825
7	81.03107966032084	2.0732179005445555	11.89156211745222	5.004140321682392
8	81.56898918997231	4.371063205366601	8.138024900412969	5.921922704248116
9	76.46343424730547	4.754657695748162	10.298568365815697	8.483339691130674
10	41.20942584437973	25.82364489198612	16.792294083792665	16.174635179841484
11	31.698116718525917	23.418403763107143	25.234297015911327	19.649182502455616
12	27.304457857107728	20.67768359967409	29.968219874341568	22.04963866887661
13	24.861335775436395	22.68112079770641	31.89164186682652	20.565901560030678
14	20.36865477426275	26.385213402679845	31.098002676920665	22.148129146136743
15	18.563128940117522	22.347236750638327	36.792905495662296	22.29672881358185
16	22.10027952155692	22.738141600330692	30.371485877050393	24.790093001061997
17	21.501361720566166	22.9757947357438	29.66729890198403	25.855544641705997
18	22.899501167663754	21.52262822037942	31.279299587828653	24.298571024128172
19	23.80518822846069	23.689019973230792	28.325914426263065	24.179877372045453
20	25.01817621155914	26.28805208165804	29.009233648387667	19.68453805839515
21	23.483665334409064	27.169282667669737	29.303375923929732	20.04367607399147
22	22.586617789161263	22.24887918900203	30.00782873024375	25.156674291592957
23	21.337210925132617	24.885659334597804	28.98318218611643	24.79394755415315
24	22.72298921921375	23.537230330813696	28.80254985332761	24.937230596644945
25	21.603440919669783	26.941066541548757	25.705615818022558	25.749876720758895
26	19.98080698391854	26.476659351876837	27.826151680651606	25.71638198355302
27	20.902576835199245	27.25195618569376	28.61979087055746	23.225676108549532
28	18.226054918077455	26.934952422852447	29.74266206069725	25.096330598372845
29	21.361401568670193	25.52192642588558	28.91326856798036	24.203403437463862
30	22.75661687204346	26.184643726316096	29.62011385552338	21.43862554611707
31	25.233233690920663	26.857994276653237	24.624613049390117	23.28415898303598
32	25.355383149223044	26.453266202082254	24.58992207156975	23.601428577124956
33	23.242689308400134	27.75171893130522	23.96548447080309	25.04010728949156
34	22.278785204364418	25.635835115510325	27.495856355427012	24.58952332469825
35	20.513798635488207	25.697242133721094	27.00752435346518	26.781434877325527
36	22.341920125685014	28.70166689483849	25.537743385121694	23.418669594354807
37	22.52786908342715	26.443430445918626	28.35661793536845	22.672082535285774
38	22.57824410485979	28.094242493922433	24.37486459220822	24.952648809009553
39	24.704628254937482	24.675519733318094	25.254234359486254	25.36561765225817
40	24.308938442787134	24.18771939385159	27.100964037019658	24.402378126341617
41	22.21219447682417	23.34463559187992	25.336243299391114	29.106926631904802
42	21.388250524684423	23.262892483222725	26.939471554062767	28.409385438030082
43	22.144673339917087	23.18447226516135	27.615081670005065	27.055772724916494
44	22.39428888147515	23.633727073716333	28.238721777028726	25.73326226777979
45	22.581035332960283	22.65533516668284	26.908369298085884	27.855260202270998
46	23.515299252881277	24.27079165874711	27.848747336703187	24.365161751668424
47	19.29642443680327	25.400042798830874	31.055203846046492	24.248328918319363
48	20.773515764457564	24.982687739995775	28.002397797853945	26.24139869769272
49	22.734419962863374	22.06146815939773	30.500812779039737	24.703299098699155
50	22.38432020968769	22.732160397258216	27.583447751532848	27.300071641521246
51	20.544635060217423	22.84872739935961	25.647930437279108	30.95870710314385
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	156.0
1	455.0
2	754.0
3	1276.5
4	1799.0
5	1356.5
6	914.0
7	918.5
8	923.0
9	1025.0
10	1127.0
11	1135.0
12	1143.0
13	1177.0
14	1211.0
15	1166.0
16	1121.0
17	1180.0
18	1239.0
19	1251.0
20	1263.0
21	1430.0
22	1597.0
23	1893.0
24	2189.0
25	2744.5
26	4214.0
27	5128.0
28	6022.5
29	6917.0
30	7916.0
31	8915.0
32	10837.5
33	12760.0
34	14951.0
35	17142.0
36	18544.0
37	19946.0
38	22330.0
39	24714.0
40	28514.0
41	32314.0
42	37106.5
43	41899.0
44	49554.0
45	57209.0
46	71414.0
47	85619.0
48	86995.0
49	88371.0
50	83192.0
51	78013.0
52	66824.5
53	55636.0
54	49394.0
55	43152.0
56	39728.5
57	36305.0
58	32861.0
59	29417.0
60	27034.0
61	24651.0
62	21720.0
63	18789.0
64	16728.0
65	14667.0
66	12505.5
67	10344.0
68	8584.5
69	6825.0
70	6213.5
71	5602.0
72	4750.5
73	3899.0
74	3339.5
75	2086.0
76	1392.0
77	1072.0
78	752.0
79	533.0
80	314.0
81	201.5
82	89.0
83	71.0
84	53.0
85	28.0
86	3.0
87	3.0
88	3.0
89	1.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	752357.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.797822344710205
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.7960652515755	27.137349194282724
2	11.197206288467571	8.464600280971384
3	4.216119967162809	4.780804605084159
4	2.287714454164324	3.4588249805571527
5	1.5481568101670802	2.925847803622427
6	1.1842254228701874	2.6856685289841997
7	0.9819757065622742	2.5981580312423485
8	0.8280206314502457	2.5037901380248924
9	0.6671884362209027	2.269644298445043
>10	5.164551693322832	34.28489964026945
>50	0.08310501577500139	2.127801536996563
>100	0.03780903728187665	2.572661683524101
>500	0.0048665097491524196	1.349013116858361
>1k	0.002994775230247643	2.840936161137211
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGC	4288	0.569942194995195	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCG	3510	0.46653383965324974	No Hit
GAATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC	2578	0.3426564782410478	No Hit
CCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	2535	0.3369411064162359	No Hit
CTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT	2062	0.27407201634330514	TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	2055	0.27314160697647527	No Hit
TCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	1625	0.21598788872835636	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCATAGAGCCT	1425	0.1894047639617894	No Hit
GAATCAGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTC	944	0.12547234889819595	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTC	917	0.12188362705470938	No Hit
CCTGTATCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	911	0.12108613331171239	TruSeq Adapter, Index 15 (95% over 22bp)
GCTGTATCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGC	878	0.11669991772522885	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCT	852	0.11324411150557515	No Hit
CTGTCACTTATACACATCTGACGCATAGAGCCTCGTATGCCGTCTTCTGCT	764	0.10154753660828571	TruSeq Adapter, Index 15 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2932118661752333	0.0
2	0.0	0.0	0.0	1.2032851425586524	0.0
3	0.0	0.0	0.0	1.660116141672105	0.0
4	0.0	0.0	0.0	2.1798162308584885	0.0
5	0.0	0.0	0.0	3.3012253491361148	0.0
6	0.0	0.0	0.0	3.9429419810010407	0.0
7	0.0	0.0	0.0	4.874414672821546	0.0
8	0.0	0.0	0.0	6.351904747347336	0.0
9	0.0	0.0	0.0	6.952284620200251	0.0
10	0.0	0.0	0.0	8.60322958382789	0.0
11	0.0	0.0	0.0	10.608793431841534	0.0
12	0.0	0.0	0.0	13.132462381555564	0.0
13	0.0	0.0	0.0	13.789995972656598	0.0
14	0.0	0.0	0.0	14.031237829913193	0.0
15	0.0	0.0	0.0	14.446599154390801	0.0
16	0.0	0.0	0.0	15.23146591312369	0.0
17	0.0	0.0	0.0	16.228598923117616	0.0
18	0.0	0.0	0.0	17.401180556570882	0.0
19	0.0	0.0	0.0	18.363888419992104	0.0
20	0.0	0.0	0.0	19.184509481536026	0.0
21	0.0	0.0	0.0	20.213143494378333	0.0
22	0.0	0.0	0.0	21.27447475068352	0.0
23	0.0	0.0	0.0	22.29898837918701	0.0
24	0.0	0.0	0.0	23.103526650247158	0.0
25	0.0	0.0	0.0	23.80266283160787	0.0
26	0.0	0.0	0.0	24.41061889501925	0.0
27	0.0	0.0	0.0	25.077190748540918	0.0
28	0.0	0.0	0.0	25.713856586700196	0.0
29	0.0	0.0	0.0	26.4242905960867	0.0
30	1.3291562383283467E-4	0.0	0.0	27.227765542156185	0.0
31	1.3291562383283467E-4	0.0	0.0	27.91321141426211	0.0
32	1.3291562383283467E-4	0.0	0.0	28.577656617802454	0.0
33	1.3291562383283467E-4	0.0	0.0	29.21006915599908	0.0
34	1.3291562383283467E-4	0.0	0.0	29.85856448467948	0.0
35	1.3291562383283467E-4	0.0	0.0	30.631070090395916	0.0
36	1.3291562383283467E-4	0.0	0.0	31.284217465910466	0.0
37	1.3291562383283467E-4	0.0	0.0	31.955707197513945	0.0
38	1.3291562383283467E-4	0.0	0.0	32.65564087261765	0.0
39	1.3291562383283467E-4	0.0	0.0	33.712452997712525	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATACGG	125	0.0	45.000004	5
ACCGAAA	20	7.0317305E-4	45.0	17
ATCGTAC	20	7.0317305E-4	45.0	10
TAACCGG	20	7.0317305E-4	45.0	31
CCATACG	20	7.0317305E-4	45.0	2
CGTACTC	20	7.0317305E-4	45.0	12
GCGATAC	20	7.0317305E-4	45.0	9
ATACGAC	20	7.0317305E-4	45.0	28
CGGTAAG	20	7.0317305E-4	45.0	14
AATCGTC	20	7.0317305E-4	45.0	18
ACGGGTA	60	0.0	44.999996	5
TAATACG	130	0.0	43.26923	4
TTCTACG	120	0.0	43.124996	1
CTTTACG	105	0.0	42.857147	1
CCGATGA	310	0.0	42.82258	18
AGACACG	155	0.0	42.09677	24
AGGCACG	145	0.0	41.89655	10
GGCGATA	70	0.0	41.785713	8
TTGACGG	135	0.0	41.666664	2
AAACACG	130	0.0	41.53846	40
>>END_MODULE