##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934099.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	814713
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.790323709085285	31.0	31.0	34.0	30.0	34.0
2	32.00712029880461	33.0	31.0	34.0	30.0	34.0
3	32.09418408691159	33.0	31.0	34.0	30.0	34.0
4	35.56974419212655	37.0	35.0	37.0	33.0	37.0
5	29.398150023382467	35.0	35.0	37.0	0.0	37.0
6	23.53544499719533	35.0	10.0	37.0	0.0	37.0
7	30.27271812282362	35.0	19.0	37.0	17.0	37.0
8	34.135759463762085	35.0	32.0	37.0	32.0	37.0
9	36.70833532790075	37.0	35.0	39.0	33.0	39.0
10	36.821332174643096	38.0	37.0	39.0	33.0	39.0
11	37.05895695784896	39.0	37.0	39.0	34.0	39.0
12	37.10830685161523	39.0	37.0	39.0	34.0	39.0
13	37.053706028994256	39.0	37.0	39.0	33.0	39.0
14	38.15535532144448	40.0	37.0	41.0	33.0	41.0
15	38.00549273179635	40.0	37.0	41.0	33.0	41.0
16	38.2164443184287	40.0	37.0	41.0	34.0	41.0
17	38.21710467367036	40.0	37.0	41.0	34.0	41.0
18	38.30455019129436	40.0	37.0	41.0	34.0	41.0
19	38.30398066558408	40.0	37.0	41.0	34.0	41.0
20	38.27954138451209	40.0	37.0	41.0	34.0	41.0
21	38.1294934535229	40.0	37.0	41.0	34.0	41.0
22	38.1925365128579	40.0	37.0	41.0	34.0	41.0
23	38.148708809114375	40.0	37.0	41.0	34.0	41.0
24	38.13243436645788	40.0	37.0	41.0	34.0	41.0
25	37.88353690195198	40.0	37.0	41.0	33.0	41.0
26	37.78516851946637	40.0	37.0	41.0	33.0	41.0
27	36.96636607001484	40.0	36.0	41.0	32.0	41.0
28	37.358278313958415	40.0	36.0	41.0	32.0	41.0
29	37.68552115898482	40.0	36.0	41.0	33.0	41.0
30	37.69978753254213	40.0	36.0	41.0	33.0	41.0
31	37.728107934941505	40.0	36.0	41.0	33.0	41.0
32	37.73344723847539	40.0	36.0	41.0	33.0	41.0
33	36.56381204178649	40.0	36.0	41.0	31.0	41.0
34	37.02528743250691	39.0	36.0	41.0	31.0	41.0
35	37.340171324135	40.0	36.0	41.0	31.0	41.0
36	37.4424269650785	39.0	36.0	41.0	33.0	41.0
37	37.5179198073432	39.0	36.0	41.0	33.0	41.0
38	37.457887624230864	39.0	36.0	41.0	33.0	41.0
39	37.51901344399807	39.0	36.0	41.0	33.0	41.0
40	37.441256000579344	39.0	36.0	41.0	33.0	41.0
41	37.37072809688811	39.0	36.0	41.0	33.0	41.0
42	37.394345002473266	39.0	35.0	41.0	33.0	41.0
43	37.23541418880023	39.0	35.0	40.0	33.0	41.0
44	37.236183784964766	39.0	35.0	40.0	33.0	41.0
45	37.23857972071146	39.0	35.0	40.0	33.0	41.0
46	37.22839822121409	39.0	35.0	40.0	33.0	41.0
47	37.19179514749366	39.0	35.0	40.0	33.0	41.0
48	37.16249157678839	39.0	35.0	40.0	33.0	41.0
49	37.196357490306404	39.0	35.0	40.0	33.0	41.0
50	37.097542324720486	39.0	35.0	40.0	33.0	41.0
51	36.383149649017504	38.0	35.0	40.0	31.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	7.0
10	6.0
11	8.0
12	12.0
13	12.0
14	17.0
15	22.0
16	23.0
17	52.0
18	107.0
19	168.0
20	324.0
21	541.0
22	859.0
23	1366.0
24	1931.0
25	2825.0
26	3814.0
27	5007.0
28	6506.0
29	8933.0
30	12480.0
31	17771.0
32	25796.0
33	40587.0
34	56300.0
35	75139.0
36	100914.0
37	150590.0
38	166718.0
39	135820.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	24.01324147276403	4.489556445030336	52.27546387500874	19.22173820719689
2	19.991948084785687	4.609353232365263	52.21237417348195	23.1863245093671
3	21.559493956767597	4.571180280663252	50.9632226317734	22.906103130795753
4	20.249953050951685	4.651208462366502	49.25525921398087	25.84357927270094
5	14.62956894022803	22.635701161022347	40.43350234990727	22.301227548842352
6	15.997289843171766	29.60600849624346	39.477214675597416	14.919486984987351
7	79.47399881921609	2.261900816606584	11.756900896389281	6.507199467788043
8	78.10934648152171	2.875613866478134	10.144063001326847	8.870976650673304
9	71.34230090841805	5.875565996860244	14.59360535550556	8.188527739216141
10	34.77322689094196	26.56530581934988	20.86747112173244	17.793996167975717
11	26.485523122866578	24.14592623414626	27.568726655889865	21.799823987097298
12	22.703086853898245	19.770274931172082	34.58088922111222	22.945748993817457
13	22.296931557493252	23.157848223853062	34.51252158735653	20.032698631297155
14	18.830312023988817	25.694937972022046	32.38625135477156	23.088498649217577
15	15.7826130183267	23.925357764022422	37.373283598027776	22.918745619623106
16	17.890840087245447	23.171227168340263	32.55821375134557	26.379718993068725
17	17.583492591869778	23.654587566419096	30.21824863479532	28.54367120691581
18	19.03516944003594	21.514692904126974	33.55746133914642	25.89267631669066
19	20.868084834782312	23.725410052374272	31.063945217518317	24.342559895325103
20	23.062231730683074	24.10014324062584	31.32784182896308	21.509783199728002
21	22.65546272122821	26.167006049983243	30.072921384585737	21.104609844202805
22	20.852496523315573	23.714731445306505	28.742882462904113	26.689889568473806
23	19.57744629090244	24.74945164738994	27.46316801131196	28.209934050395663
24	21.38446299494423	22.962810216603884	29.08337046297285	26.56935632547903
25	19.28482790872369	24.515504232778905	26.83202551082406	29.36764234767335
26	17.99001611610469	24.91527691346523	28.780809929386177	28.3138970410439
27	19.060208932470697	25.279945207698905	30.9063437063113	24.75350215351909
28	16.56804297955231	21.797737362727734	34.63698259387048	26.99723706384948
29	17.769693131200803	20.538152699171363	33.40047354098928	28.29168062863855
30	19.7319792368601	21.336102406614355	32.3917747722204	26.54014358430515
31	20.51237675107676	23.533563352984423	30.714865234751375	25.239194661187437
32	21.630561927942722	23.482625169845086	28.743987146393884	26.142825755818304
33	22.181921731947323	23.257024252712306	28.4390945032177	26.12195951212267
34	20.186986092034864	19.742903329147808	32.97296103044876	27.097149548368566
35	20.646902651608602	18.595259925888012	31.065172643618062	29.692664778885323
36	22.982203548979825	20.006554455372626	30.989317710653935	26.021924284993613
37	20.017601290270314	21.791477489619044	36.04643598420548	22.144485235905158
38	20.56871560905497	22.714379174015882	32.37176772679459	24.345137490134565
39	22.22623181414805	19.890194461116984	32.3569158709877	25.52665785374727
40	22.91567705437375	18.714197514953117	31.89969964883339	26.47042578183974
41	21.87396052352178	19.742903329147808	29.34738981702759	29.03574633030282
42	25.615400760758693	18.86467995478162	29.22489269227323	26.29502659218645
43	24.888028054050938	18.36057605561713	31.560070847034478	25.191325043297457
44	20.155195756051516	18.574884652632274	32.579816450701045	28.690103140615165
45	20.399453549900397	17.784054016567797	30.976552479216608	30.8399399543152
46	22.206715739162135	18.04463657754326	33.9254436838494	25.823203999445205
47	18.732363421229316	19.52393051295364	35.843174222088024	25.90053184372902
48	19.723141768941947	18.776428018210094	34.29698556424164	27.203444648606318
49	22.50019331961071	16.291135651450265	36.16500534544066	25.04366568349836
50	22.36640387473871	16.244370717050053	32.88139504340792	28.50783036480331
51	20.472117175005184	16.47696796295137	29.51112845873332	33.539786403310124
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	195.0
1	514.0
2	833.0
3	1460.0
4	2087.0
5	1483.5
6	880.0
7	974.0
8	1068.0
9	1133.0
10	1198.0
11	1307.0
12	1416.0
13	1427.5
14	1439.0
15	1454.5
16	1470.0
17	1454.0
18	1438.0
19	1537.0
20	1636.0
21	1848.5
22	2061.0
23	2296.5
24	2532.0
25	2768.0
26	3767.0
27	4530.0
28	5603.5
29	6677.0
30	7559.5
31	8442.0
32	9880.5
33	11319.0
34	13127.5
35	14936.0
36	16306.5
37	17677.0
38	20689.5
39	23702.0
40	28213.5
41	32725.0
42	39070.0
43	45415.0
44	55565.0
45	65715.0
46	80396.0
47	95077.0
48	109033.0
49	122989.0
50	112610.5
51	102232.0
52	83439.5
53	64647.0
54	53835.5
55	43024.0
56	37996.0
57	32968.0
58	29676.0
59	26384.0
60	23681.5
61	20979.0
62	18903.0
63	16827.0
64	14559.5
65	12292.0
66	10345.5
67	8399.0
68	6877.5
69	5356.0
70	4780.5
71	4205.0
72	3567.5
73	2930.0
74	2352.5
75	1400.5
76	1026.0
77	793.5
78	561.0
79	496.0
80	431.0
81	281.5
82	132.0
83	89.0
84	46.0
85	32.0
86	18.0
87	15.5
88	13.0
89	8.5
90	4.0
91	3.0
92	2.0
93	1.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	814713.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.45139565862019
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.83376530527332	28.653246663534542
2	12.457678219670191	10.078609413033082
3	4.9941824825100944	6.060649547740975
4	2.5770582198590275	4.169824067472678
5	1.7063723548019476	3.451257163251251
6	1.2478556215605323	3.028650088354711
7	0.896545492141275	2.5386561499991354
8	0.7012894011777306	2.269450803058977
9	0.5767058375353438	2.0995700411500318
>10	3.843290715119658	26.121304588882715
>50	0.10464111570602098	2.9591433892704537
>100	0.05359662852812329	3.9798067747972405
>500	0.0038283306091430277	1.1196473159991212
>1k	0.002552220406095352	2.080045578523955
>5k	6.38055101523838E-4	1.3901384149311358
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	5725	0.7027014421029246	No Hit
CTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT	5047	0.6194819525403424	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	3706	0.4548841125647928	No Hit
GCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	3165	0.38848036056869106	No Hit
CCTGTATCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	2017	0.2475718443181832	No Hit
CTGTCACTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT	1808	0.22191863883355245	Illumina Single End Adapter 2 (95% over 21bp)
TCTGTATCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	1649	0.20240256384763716	No Hit
GCTGTATCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	1395	0.1712259409141624	No Hit
TCCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	1349	0.16557978085534414	No Hit
CGCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	1029	0.12630214566356496	No Hit
CCTGACTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	919	0.11280045856639086	No Hit
CTGTATCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGCT	843	0.10347202020834331	Illumina Single End Adapter 2 (95% over 21bp)
GCCTGTCTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTG	843	0.10347202020834331	No Hit
TCTGACTCTTATACACATCTGACGCCTTTCTGGTCGTATGCCGTCTTCTGC	835	0.10249007932854884	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.6534816555032263	0.0
2	0.0	0.0	0.0	2.3247450329134307	0.0
3	0.0	0.0	0.0	3.159394780738739	0.0
4	0.0	0.0	0.0	4.054556635281381	0.0
5	1.2274260997430998E-4	0.0	0.0	5.4393387610115465	0.0
6	1.2274260997430998E-4	0.0	0.0	6.542058369020747	0.0
7	1.2274260997430998E-4	0.0	0.0	7.85528155313589	0.0
8	1.2274260997430998E-4	0.0	0.0	10.127124521150392	0.0
9	1.2274260997430998E-4	0.0	0.0	11.178046747750434	0.0
10	1.2274260997430998E-4	0.0	0.0	13.04042036889064	0.0
11	1.2274260997430998E-4	0.0	0.0	16.256890463267432	0.0
12	1.2274260997430998E-4	0.0	0.0	19.405238409108485	0.0
13	1.2274260997430998E-4	0.0	0.0	20.397735153360752	0.0
14	1.2274260997430998E-4	0.0	0.0	20.759825852784967	0.0
15	1.2274260997430998E-4	0.0	0.0	21.252514689221847	0.0
16	1.2274260997430998E-4	0.0	0.0	22.440785896383144	0.0
17	1.2274260997430998E-4	0.0	0.0	24.104930202414838	0.0
18	1.2274260997430998E-4	0.0	0.0	26.050155085287702	0.0
19	1.2274260997430998E-4	0.0	0.0	27.229220596700923	0.0
20	1.2274260997430998E-4	0.0	0.0	28.503166145624288	0.0
21	1.2274260997430998E-4	0.0	0.0	30.04162201904229	0.0
22	1.2274260997430998E-4	0.0	0.0	31.47218713829287	0.0
23	1.2274260997430998E-4	0.0	0.0	32.8499729352545	0.0
24	1.2274260997430998E-4	0.0	0.0	33.929985160418454	0.0
25	1.2274260997430998E-4	0.0	0.0	34.82551524279102	0.0
26	1.2274260997430998E-4	0.0	0.0	35.64887267049869	0.0
27	1.2274260997430998E-4	0.0	0.0	36.41441832890846	0.0
28	1.2274260997430998E-4	0.0	0.0	37.26441090298056	0.0
29	1.2274260997430998E-4	0.0	0.0	38.12090883538129	0.0
30	1.2274260997430998E-4	0.0	0.0	39.047983768517255	0.0
31	1.2274260997430998E-4	0.0	0.0	39.89539874777989	0.0
32	1.2274260997430998E-4	0.0	0.0	40.70549997361034	0.0
33	1.2274260997430998E-4	0.0	0.0	41.454475379673575	0.0
34	1.2274260997430998E-4	0.0	0.0	42.2876522161792	0.0
35	1.2274260997430998E-4	0.0	0.0	43.08376078447257	0.0
36	1.2274260997430998E-4	0.0	0.0	43.86845429003833	0.0
37	1.2274260997430998E-4	0.0	0.0	44.663703660061884	0.0
38	1.2274260997430998E-4	0.0	0.0	45.404946281696745	0.0
39	1.2274260997430998E-4	0.0	0.0	46.17847020975485	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCGATAC	35	1.2113378E-7	45.000004	9
TCGCCGT	20	7.032077E-4	45.0	25
AACCGGT	20	7.032077E-4	45.0	28
GACACGC	20	7.032077E-4	45.0	10
ATTCGGA	20	7.032077E-4	45.0	12
ATTCGAA	40	6.8102963E-9	45.0	42
CGATTCG	55	1.8189894E-12	45.0	10
CGTTCAT	95	0.0	45.0	17
AATATCG	20	7.032077E-4	45.0	14
TATTGCG	25	3.8898015E-5	45.0	1
GTTACGT	20	7.032077E-4	45.0	45
ATATCGT	20	7.032077E-4	45.0	15
CCGTAGA	50	2.1827873E-11	45.0	41
TGATTCG	25	3.8898015E-5	45.0	15
ACCGTTA	25	3.8898015E-5	45.0	34
GCGTCGA	25	3.8898015E-5	45.0	32
GGTAGTC	20	7.032077E-4	45.0	8
CGGACTA	20	7.032077E-4	45.0	34
CGCGCAT	60	0.0	44.999996	36
GTCCCTA	30	2.164721E-6	44.999996	24
>>END_MODULE