##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934097.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	283256
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.080838534753013	31.0	30.0	31.0	26.0	34.0
2	30.303139209760783	31.0	30.0	33.0	26.0	34.0
3	30.444890840794194	31.0	30.0	34.0	26.0	34.0
4	34.03419168525998	35.0	33.0	37.0	30.0	37.0
5	34.443429265399494	35.0	35.0	37.0	32.0	37.0
6	33.96582949699212	35.0	35.0	37.0	30.0	37.0
7	34.14554325415878	35.0	35.0	37.0	31.0	37.0
8	34.30476318242155	35.0	35.0	37.0	31.0	37.0
9	35.69925085435084	37.0	35.0	39.0	30.0	39.0
10	35.34441635834722	37.0	34.0	39.0	30.0	39.0
11	35.52249908210241	37.0	35.0	39.0	30.0	39.0
12	35.52886081848222	37.0	35.0	39.0	30.0	39.0
13	35.4888899087751	37.0	35.0	39.0	30.0	39.0
14	36.30779930522213	38.0	35.0	40.0	30.0	41.0
15	36.654136187759484	38.0	35.0	40.0	31.0	41.0
16	36.78307608664953	38.0	35.0	40.0	31.0	41.0
17	36.54547829525235	38.0	35.0	40.0	31.0	41.0
18	36.6515413618776	38.0	35.0	40.0	31.0	41.0
19	36.62657454740588	38.0	35.0	40.0	31.0	41.0
20	36.59382678566385	38.0	35.0	40.0	31.0	41.0
21	36.330852656254415	38.0	35.0	40.0	30.0	41.0
22	36.48985017087017	38.0	35.0	40.0	30.0	41.0
23	36.50416231253707	38.0	35.0	40.0	30.0	41.0
24	36.44652893495636	38.0	35.0	40.0	30.0	41.0
25	35.99490213799531	38.0	34.0	40.0	29.0	41.0
26	36.08253311492078	38.0	34.0	40.0	30.0	41.0
27	36.05061146101053	38.0	34.0	40.0	30.0	41.0
28	35.93042336261191	38.0	34.0	40.0	29.0	41.0
29	36.052348405682494	38.0	34.0	40.0	30.0	41.0
30	35.66533453836812	38.0	34.0	40.0	29.0	41.0
31	35.81171802186008	38.0	34.0	40.0	29.0	41.0
32	35.79545711300025	38.0	34.0	40.0	29.0	41.0
33	35.835915214505604	38.0	34.0	40.0	29.0	41.0
34	35.77873019459429	38.0	34.0	40.0	29.0	41.0
35	35.78194989691304	38.0	34.0	40.0	29.0	41.0
36	35.59439517609512	38.0	34.0	40.0	28.0	41.0
37	35.61250953201344	38.0	34.0	40.0	29.0	41.0
38	35.563560877792526	38.0	34.0	40.0	28.0	41.0
39	35.4974899031265	38.0	34.0	40.0	28.0	41.0
40	35.34985666676081	38.0	34.0	40.0	27.0	41.0
41	35.20897350806338	37.0	34.0	40.0	27.0	41.0
42	35.355328748552544	38.0	34.0	40.0	28.0	41.0
43	35.34139082667269	38.0	34.0	40.0	28.0	41.0
44	35.32326941000367	37.0	34.0	40.0	28.0	41.0
45	35.38854605021606	38.0	34.0	40.0	28.0	41.0
46	35.30925381986613	37.0	34.0	40.0	28.0	41.0
47	35.235451323184684	37.0	34.0	40.0	28.0	41.0
48	35.1880383822408	37.0	34.0	40.0	28.0	41.0
49	35.16134168384783	37.0	34.0	40.0	28.0	41.0
50	34.995223402152114	37.0	34.0	40.0	27.0	41.0
51	33.89459358318977	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	9.0
11	7.0
12	18.0
13	16.0
14	13.0
15	28.0
16	34.0
17	75.0
18	176.0
19	274.0
20	496.0
21	832.0
22	1240.0
23	1676.0
24	2185.0
25	2693.0
26	3321.0
27	4077.0
28	4993.0
29	6380.0
30	8209.0
31	10263.0
32	13381.0
33	18101.0
34	25724.0
35	26534.0
36	31535.0
37	40487.0
38	51539.0
39	28938.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.805631654757534	5.272615584488943	50.79045104075466	18.13130171999887
2	19.93567656113198	6.1093145423221396	53.437526477815126	20.517482418730758
3	22.289377806648403	5.360168893156721	50.011297201118424	22.339156099076455
4	19.83153048832152	5.459019402942921	48.16526393086113	26.54418617787443
5	19.618648854746237	6.017524783234954	48.08441833535741	26.279408026661393
6	22.217005111983507	8.214477363233259	51.831911768859264	17.73660575592397
7	85.03473889343915	2.94856949190838	8.246250741378823	3.7704408732736465
8	86.26931115316181	4.931581325726551	4.901573135255741	3.897534385855904
9	80.77710622193351	6.249470443697573	6.838690089530319	6.134733244838591
10	42.1618606490242	30.47843646736521	13.959104131951309	13.400598751659276
11	34.10589713898381	26.3309515067642	21.689920072302087	17.8732312819499
12	31.644519445307424	21.67721072104386	26.851681870816506	19.82658796283221
13	27.064563504391785	24.11634705001836	27.308865478577683	21.510223967012173
14	19.74750755500325	28.76267404750473	27.424661790041515	24.065156607450504
15	18.67250826107832	25.810221142711892	34.37738300336092	21.139887592848872
16	21.449854548535598	22.85141356228994	33.4799615895162	22.21877029965826
17	20.923475583924084	23.83109272177818	28.914127149998585	26.33130454429915
18	21.99706272770921	24.464795097014715	28.529669274437257	25.008472900838818
19	25.687011043014092	27.01196091168413	25.570155618945407	21.73087242635637
20	30.904552772050724	23.592792385686444	26.232454034512948	19.27020080774988
21	27.596238032027564	25.96096817013585	25.031773378145566	21.41102041969102
22	24.923037817380745	24.44679018273223	26.265286525263363	24.364885474623662
23	25.428234529895217	26.812494704436972	26.227158471488686	21.532112294179118
24	24.233202474087044	24.54846499279803	28.235589007823314	22.98274352529161
25	24.154475103793036	26.841090744767982	25.600869884486116	23.403564266952863
26	22.869065439037477	28.5271980116926	25.923546191431075	22.680190357838846
27	23.82650323382382	28.705128929307765	26.419916965571776	21.048450871296637
28	22.375518965176376	29.088174654729293	27.655548337899283	20.880758042195048
29	23.265173553252183	26.780721326291413	27.417954076877454	22.536151043578954
30	24.055271556471883	27.111517496540234	25.34491767164685	23.488293275341036
31	23.198096421611545	28.32420142909594	24.449967520546785	24.02773462874573
32	25.325853644759512	28.913421074928685	23.937003982263395	21.823721298048408
33	25.34068122122744	28.692772615584488	24.165066229841557	21.801479933346513
34	23.523950066371057	28.30478436467365	25.33926907108764	22.831996497867653
35	24.23214336148219	26.3330697319739	24.99541051204564	24.439376394498264
36	23.140904340949533	29.125243595899114	24.09692998559607	23.636922077555287
37	24.00019770101957	29.277755810997824	25.3689242240235	21.353122263959104
38	23.326602084333604	27.398537012455165	24.379360013556642	24.89550088965459
39	23.305419832236563	27.579998305419835	25.402815827378767	23.71176603496484
40	24.928333380405004	25.306789617872173	27.80558929025334	21.959287711469482
41	19.33904312706527	27.593060694213005	28.02906204987714	25.038834128844577
42	23.12184031406219	27.659431750783742	25.50590278758438	23.71282514756969
43	23.163145705651424	27.718035981585565	25.420467704126303	23.69835060863671
44	23.066060383539977	27.390417149151297	26.68257689157511	22.860945575733613
45	22.911076905696614	27.141172649476093	24.243087525065665	25.704662919761628
46	21.941282797186997	29.66256672409411	25.052602592707657	23.34354788601124
47	21.990708052080095	28.967788855311095	26.70234699353235	22.339156099076455
48	22.5100262659926	26.088061682718106	27.267560086988446	24.134351964300844
49	21.013853192871466	26.649744400824694	27.542576326715057	24.793826079588783
50	20.19233484904115	27.85607365775129	26.758126924054565	25.19346456915299
51	20.239994916259498	27.81759256644166	25.96802892083486	25.974383596463973
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	33.0
1	177.0
2	321.0
3	425.5
4	530.0
5	428.5
6	327.0
7	346.5
8	366.0
9	367.0
10	368.0
11	385.0
12	402.0
13	434.0
14	466.0
15	465.5
16	465.0
17	466.5
18	468.0
19	498.5
20	529.0
21	642.0
22	755.0
23	917.5
24	1080.0
25	1394.0
26	2446.5
27	3185.0
28	3488.0
29	3791.0
30	4423.5
31	5056.0
32	5814.0
33	6572.0
34	7073.0
35	7574.0
36	8896.0
37	10218.0
38	10478.0
39	10738.0
40	13100.0
41	15462.0
42	16020.0
43	16578.0
44	17949.5
45	19321.0
46	20090.5
47	20860.0
48	20789.0
49	20718.0
50	20592.0
51	20466.0
52	19285.0
53	18104.0
54	16933.5
55	15763.0
56	15910.5
57	16058.0
58	16094.0
59	16130.0
60	15720.5
61	15311.0
62	13610.5
63	11910.0
64	9835.5
65	7761.0
66	6411.0
67	5061.0
68	4228.5
69	3396.0
70	2776.5
71	2157.0
72	1809.5
73	1462.0
74	1130.5
75	666.0
76	533.0
77	409.5
78	286.0
79	191.0
80	96.0
81	68.5
82	41.0
83	28.5
84	16.0
85	15.0
86	14.0
87	7.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	283256.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.838068743468806
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.6648447110446	21.063631485299517
2	7.455645314699503	4.151015335950518
3	2.343601384855364	1.9572400937667693
4	1.29100985377855	1.4375688423193154
5	0.8649005110775747	1.2038579941819414
6	0.7203276983754581	1.20315191911204
7	0.618873092970464	1.2059762193916457
8	0.6239458232407138	1.389555737566018
9	0.5377094086464688	1.347191233371932
>10	9.612823862123191	58.29108650831757
>50	0.21685921905317487	3.922953088372356
>100	0.0481909375673722	2.6234219222187702
>500	0.0012681825675624264	0.2033496201316124
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	576	0.2033496201316124	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGC	470	0.1659276414268365	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCG	469	0.16557460389188577	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	408	0.1440393142598921	No Hit
CCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC	375	0.1323890756065185	Illumina PCR Primer Index 9 (95% over 24bp)
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	352	0.12426921230265203	No Hit
GGTTCTGGAGGAGCTACTCTAGTATTAATAAATATTAGCCCACCAACAGCT	328	0.11579631146383484	No Hit
GCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC	322	0.11367808625413053	Illumina PCR Primer Index 9 (95% over 24bp)
GAATCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTC	288	0.1016748100658062	Illumina PCR Primer Index 9 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGCT	284	0.10026265992600332	Illumina PCR Primer Index 9 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12356313723275059	0.0
2	0.0	0.0	0.0	0.5352049029852854	0.0
3	0.0	0.0	0.0	0.6866580054791426	0.0
4	0.0	0.0	0.0	0.9111898777077979	0.0
5	0.0	0.0	0.0	1.3627248849097635	0.0
6	0.0	0.0	0.0	1.5787838562996017	0.0
7	0.0	0.0	0.0	1.7906063772700314	0.0
8	0.0	0.0	0.0	2.1041037083062673	0.0
9	0.0	0.0	0.0	2.2191939447002005	0.0
10	0.0	0.0	0.0	2.6530770751546306	0.0
11	0.0	0.0	0.0	3.137091535572062	0.0
12	0.0	0.0	0.0	3.8053915892337673	0.0
13	0.0	0.0	0.0	4.000621346061513	0.0
14	0.0	0.0	0.0	4.095941480498206	0.0
15	0.0	0.0	0.0	4.237862569548394	0.0
16	0.0	0.0	0.0	4.487813144293502	0.0
17	0.0	0.0	0.0	4.82743525291609	0.0
18	0.0	0.0	0.0	5.186474425960968	0.0
19	0.0	0.0	0.0	5.4978535317874995	0.0
20	0.0	0.0	0.0	5.743214618578247	0.0
21	0.0	0.0	0.0	6.093074815714407	0.0
22	0.0	0.0	0.0	6.456350439178694	0.0
23	0.0	0.0	0.0	6.81962606264298	0.0
24	0.0	0.0	0.0	7.097113565114243	0.0
25	0.0	0.0	0.0	7.369305504561245	0.0
26	0.0	0.0	0.0	7.6224334171209085	0.0
27	0.0	0.0	0.0	7.8575564153980855	0.0
28	0.0	0.0	0.0	8.104682689863587	0.0
29	0.0	0.0	0.0	8.393467393453271	0.0
30	0.0	0.0	0.0	8.702728274070099	0.0
31	0.0	0.0	0.0	8.991866015194736	0.0
32	0.0	0.0	0.0	9.311718021860084	0.0
33	0.0	0.0	0.0	9.586381224051742	0.0
34	0.0	0.0	0.0	9.88152060327054	0.0
35	0.0	0.0	0.0	10.19219363402717	0.0
36	0.0	0.0	0.0	10.512045640692518	0.0
37	0.0	0.0	0.0	10.865436213178185	0.0
38	0.0	0.0	0.0	11.183169994633829	0.0
39	0.0	0.0	0.0	11.487488349761346	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCATAAG	20	7.024278E-4	45.0	30
CGGGTTC	20	7.024278E-4	45.0	6
CGGGTAC	40	6.7830115E-9	45.0	6
GGTACCT	40	6.7830115E-9	45.0	8
CCCTCGT	20	7.024278E-4	45.0	14
CGCGGAA	25	3.883339E-5	45.0	41
GCGCGAC	20	7.024278E-4	45.0	9
GGCGTGA	20	7.024278E-4	45.0	16
TCGGGTT	20	7.024278E-4	45.0	5
CGCGACC	20	7.024278E-4	45.0	10
TCGATCA	20	7.024278E-4	45.0	17
ACGGGTA	20	7.024278E-4	45.0	5
AGACGAG	20	7.024278E-4	45.0	24
TATCTCG	20	7.024278E-4	45.0	1
AGCATAA	20	7.024278E-4	45.0	29
GCTAGTC	25	3.883339E-5	45.0	27
CTCGATC	20	7.024278E-4	45.0	16
GCGTGAT	20	7.024278E-4	45.0	17
GGCGCAT	20	7.024278E-4	45.0	18
GCTACGA	70	0.0	45.0	10
>>END_MODULE