SRR2934096.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934096.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	546747
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGC	4222	0.772203596910454	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCG	3821	0.6988607161996316	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTC	2476	0.4528602808977461	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCGATTT	1850	0.3383649110100284	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	1056	0.19314234920356216	Illumina Paired End PCR Primer 2 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTA	838	0.153270159689948	No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	772	0.14119876286472535	Illumina Paired End PCR Primer 2 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTC	749	0.13699206397108718	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT	690	0.12620096680914572	Illumina Paired End PCR Primer 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT	663	0.12126266810791829	TruSeq Adapter, Index 1 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCT	656	0.1199823684446371	No Hit

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	100	0.0	45.000004	40
TGTTCCG	35	1.2103737E-7	45.000004	1
CGATCGA	100	0.0	45.000004	41
CGACGGT	20	7.030038E-4	45.0	28
CGTTGAT	20	7.030038E-4	45.0	25
CACGCTA	20	7.030038E-4	45.0	14
CGGTCTA	20	7.030038E-4	45.0	31
ACCCGTT	20	7.030038E-4	45.0	10
TCGCGGA	20	7.030038E-4	45.0	3
ACGACGG	20	7.030038E-4	45.0	27
TGCGACC	30	2.163406E-6	44.999996	32
TTTCGCG	155	0.0	42.096775	1
CGATGAA	1145	0.0	41.65939	19
TCGGCGT	65	0.0	41.53846	4
ACCCCCG	170	0.0	41.02941	36
GGCCGAT	160	0.0	40.78125	8
CCGATCA	50	1.0786607E-9	40.500004	40
CCGATGA	1170	0.0	40.384617	18
ACGGGAT	45	1.9250365E-8	40.0	5
GTGCTCG	45	1.9250365E-8	40.0	1