##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934096.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	546747
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.019336182914582	31.0	30.0	31.0	26.0	34.0
2	30.022953943963113	31.0	30.0	33.0	25.0	34.0
3	30.109156520291837	31.0	30.0	34.0	25.0	34.0
4	33.9841133101782	35.0	33.0	37.0	28.0	37.0
5	34.48155728335043	35.0	35.0	37.0	32.0	37.0
6	33.81121798564967	35.0	35.0	37.0	29.0	37.0
7	34.45935871618866	35.0	35.0	37.0	32.0	37.0
8	34.39376164844069	35.0	35.0	37.0	31.0	37.0
9	35.96237930889424	37.0	35.0	39.0	31.0	39.0
10	35.33995431159202	37.0	34.0	39.0	30.0	39.0
11	35.46702222417316	37.0	35.0	39.0	30.0	39.0
12	35.618900515229164	37.0	35.0	39.0	30.0	39.0
13	35.64245254203498	37.0	35.0	39.0	30.0	39.0
14	36.421070440258475	38.0	35.0	40.0	30.0	41.0
15	36.622796284204576	38.0	35.0	40.0	31.0	41.0
16	36.841850069593434	38.0	35.0	40.0	31.0	41.0
17	36.465758385505545	38.0	35.0	40.0	30.0	41.0
18	36.62875150663835	38.0	35.0	40.0	31.0	41.0
19	36.620822793723605	38.0	35.0	40.0	30.0	41.0
20	36.532959485831654	38.0	35.0	40.0	30.0	41.0
21	36.37050043255839	38.0	35.0	40.0	30.0	41.0
22	36.54358231503785	38.0	35.0	40.0	30.0	41.0
23	36.47373282340827	38.0	35.0	40.0	30.0	41.0
24	36.42834437134543	38.0	35.0	40.0	30.0	41.0
25	36.0625536125484	38.0	34.0	40.0	30.0	41.0
26	36.157304932628804	38.0	34.0	40.0	30.0	41.0
27	36.111557996660245	38.0	34.0	40.0	30.0	41.0
28	35.999454958143346	38.0	34.0	40.0	29.0	41.0
29	36.16336440803516	38.0	35.0	40.0	30.0	41.0
30	35.739696788459746	38.0	34.0	40.0	29.0	41.0
31	35.97135238053433	38.0	34.0	40.0	30.0	41.0
32	35.87849407495606	38.0	34.0	40.0	29.0	41.0
33	35.86849676358535	38.0	34.0	40.0	29.0	41.0
34	35.806118734990775	38.0	34.0	40.0	29.0	41.0
35	35.87633036852511	38.0	35.0	40.0	29.0	41.0
36	35.58287836970299	38.0	34.0	40.0	27.0	41.0
37	35.67191955328516	38.0	34.0	40.0	28.0	41.0
38	35.683252034304715	38.0	34.0	40.0	28.0	41.0
39	35.5461703493572	38.0	34.0	40.0	28.0	41.0
40	35.48956281424498	38.0	34.0	40.0	27.0	41.0
41	35.29833542753778	38.0	34.0	40.0	27.0	41.0
42	35.46199613349501	38.0	34.0	40.0	28.0	41.0
43	35.4309634986566	38.0	34.0	40.0	28.0	41.0
44	35.50489714621205	38.0	34.0	40.0	28.0	41.0
45	35.5208752860098	38.0	34.0	40.0	28.0	41.0
46	35.4351958035435	38.0	34.0	40.0	28.0	41.0
47	35.323625003886626	38.0	34.0	40.0	27.0	41.0
48	35.31074153127498	38.0	34.0	40.0	27.0	41.0
49	35.35623972330895	38.0	34.0	40.0	28.0	41.0
50	35.15647090884815	38.0	34.0	40.0	27.0	41.0
51	34.15354999661635	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	6.0
12	9.0
13	13.0
14	17.0
15	24.0
16	47.0
17	92.0
18	235.0
19	531.0
20	1231.0
21	1959.0
22	3013.0
23	3970.0
24	4832.0
25	5227.0
26	5942.0
27	7275.0
28	9061.0
29	11584.0
30	15272.0
31	19837.0
32	25159.0
33	33327.0
34	44673.0
35	49462.0
36	61476.0
37	80369.0
38	104020.0
39	58078.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.434626984693107	4.210905592531829	52.3443201334438	16.010147289331265
2	18.95666551439697	9.101833206217867	52.106915995881096	19.83458528350407
3	20.436509025198127	9.127987899339182	50.69328226766676	19.742220807795928
4	18.893565030992395	4.380819647844433	51.33654139848961	25.38907392267356
5	21.916901235854976	5.5500990403239525	48.64443700651307	23.888562717308005
6	21.141954139666062	10.974362913742555	51.283866212343185	16.5998167342482
7	83.4545045514653	1.7746782332596245	11.645971537109485	3.1248456781655864
8	85.39836524022995	6.565742473209729	4.6961391649154	3.339753121644929
9	80.58187790696611	4.851055424172424	5.795367875818249	8.771698793043218
10	39.11608111247067	36.21986037417672	12.635094476970151	12.028964036382458
11	27.64496192937501	24.83708186784747	29.741909877877703	17.776046324899816
12	24.57297433730775	22.666425238730163	30.648727839384577	22.11187258457751
13	25.0662555075748	23.014849647094543	33.345221830206654	18.573673015123997
14	20.229649179602266	28.55177989088189	30.135327674408824	21.083243255107025
15	17.43420631480374	26.19291921126225	34.390129255396005	21.982745218538007
16	20.428461427314645	25.518201288713062	30.783159304029102	23.270177979943192
17	20.90455000210335	26.64047539355497	30.957462958187243	21.49751164615444
18	20.451141021349912	24.37160149026881	31.065373929806654	24.111883558574622
19	21.834596257501186	25.998862362299196	29.713926185237415	22.452615194962203
20	26.839836341123043	25.72707303377979	30.06454539302456	17.368545232072606
21	24.99272972691208	28.255299068856342	28.24597117130958	18.50600003292199
22	22.44456759707872	23.716270962620737	31.70479216163966	22.134369278660877
23	22.631491347917777	27.240387235778158	29.25009190722583	20.87802950907824
24	21.18749622768849	27.181493451267226	30.0211981044249	21.609812216619385
25	20.20824988523028	30.05704649499677	28.517211799973296	21.21749181979965
26	19.883602470612548	27.90175346183884	30.725180019277655	21.489464048270957
27	22.741780018911857	27.81670498420659	28.756627837006874	20.684887159874677
28	18.40394185976329	29.208756518097033	31.624864882660535	20.76243673947914
29	19.994256941510425	25.17050848015627	32.73982298942656	22.095411588906753
30	21.775336673086453	28.278344462795406	29.71831578408295	20.22800308003519
31	21.71863768799829	26.571156311785888	27.314095916392773	24.396110083823046
32	22.93473946816352	27.967597444521875	28.225120576793287	20.87254251052132
33	23.609091590808912	26.376002063111457	27.40408269272625	22.61082365335338
34	20.74176904491474	26.91811752053509	29.03628186345787	23.303831571092296
35	22.986865954454256	24.794831978959188	29.796048263639307	22.42225380294725
36	20.819135724567303	27.98003464125089	29.110905043831973	22.08992459034983
37	20.761156439815856	26.94390641375261	28.735228542634893	23.559708603796636
38	20.550272795278254	26.36082136710398	27.736594805275566	25.3523110323422
39	20.339389150740654	27.899741562367968	28.3807684358579	23.380100851033475
40	22.052430100210884	24.818791872657737	30.07058109143717	23.058196935694205
41	18.987392706315717	26.94134581442605	30.148862270849225	23.922399208409008
42	20.534543399415085	25.508690491214402	30.990019149624963	22.96674695974555
43	21.118542945823204	26.43105494863255	29.71758418427536	22.732817921268886
44	21.635052409981217	25.841202603763712	29.78617166623685	22.737573320018218
45	21.030750968912496	24.856469262748586	28.805462124163462	25.30731764417546
46	22.355861120408527	26.630964596056312	28.40948372830578	22.603690555229385
47	19.498598071868706	27.434626984693107	31.061167230913018	22.005607712525173
48	20.161061697640772	26.87477023193543	30.054302995718313	22.909865074705486
49	20.218675182488425	24.446590470546706	31.240957883628077	24.09377646333679
50	20.126310706780284	25.51710388900168	30.137156673927795	24.219428730290243
51	19.964992949206856	24.725512897190107	29.787269065948234	25.522225087654803
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	45.0
1	258.0
2	471.0
3	733.0
4	995.0
5	805.0
6	615.0
7	632.0
8	649.0
9	670.0
10	691.0
11	716.0
12	741.0
13	775.5
14	810.0
15	845.0
16	880.0
17	1032.5
18	1185.0
19	1508.0
20	1831.0
21	2170.0
22	2509.0
23	2623.5
24	2738.0
25	4074.0
26	6622.0
27	7834.0
28	9529.5
29	11225.0
30	12507.5
31	13790.0
32	15245.5
33	16701.0
34	19060.0
35	21419.0
36	22842.0
37	24265.0
38	25398.0
39	26531.0
40	28991.0
41	31451.0
42	32965.5
43	34480.0
44	36007.0
45	37534.0
46	46259.5
47	54985.0
48	51124.5
49	47264.0
50	45220.0
51	43176.0
52	39251.5
53	35327.0
54	31846.0
55	28365.0
56	26111.0
57	23857.0
58	22129.0
59	20401.0
60	19086.5
61	17772.0
62	14510.0
63	11248.0
64	9463.5
65	7679.0
66	6006.5
67	4334.0
68	3609.0
69	2884.0
70	2312.5
71	1741.0
72	1579.5
73	1418.0
74	1086.5
75	577.5
76	400.0
77	285.0
78	170.0
79	129.0
80	88.0
81	69.5
82	51.0
83	36.5
84	22.0
85	16.0
86	10.0
87	5.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	546747.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.546500544261413
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.10487517668426	21.895397347403254
2	8.71835291571835	5.151936383386706
3	2.9138823478181175	2.5828507912716514
4	1.5281573012855378	1.8060680213660074
5	1.008169401596135	1.4893938886483953
6	0.7977064117237612	1.4141659756894158
7	0.6725817448310631	1.3910705822797906
8	0.6027987226543124	1.424847422958858
9	0.5574111322286351	1.4822593489593665
>10	8.9259553475061	53.570008927054324
>50	0.11989267198955951	2.3500451910469553
>100	0.0426843020695814	2.0447674399252374
>500	0.004393972271868673	0.9073017973799783
>1k	0.003138551622763338	2.4898868826300506
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGC	4222	0.772203596910454	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCG	3821	0.6988607161996316	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTC	2476	0.4528602808977461	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGCCGATTT	1850	0.3383649110100284	No Hit
GCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	1056	0.19314234920356216	Illumina Paired End PCR Primer 2 (95% over 24bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTA	838	0.153270159689948	No Hit
CCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGC	772	0.14119876286472535	Illumina Paired End PCR Primer 2 (95% over 24bp)
GAATGATCTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTC	749	0.13699206397108718	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCT	690	0.12620096680914572	Illumina Paired End PCR Primer 2 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCTTCTGCT	663	0.12126266810791829	TruSeq Adapter, Index 1 (95% over 24bp)
GAATGACTGTCTCTTATACACATCTGACGCTGCCGATTTCGTATGCCGTCT	656	0.1199823684446371	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1331511649812436	0.0
2	0.0	0.0	0.0	0.6205795367875818	0.0
3	0.0	0.0	0.0	0.7895790923406987	0.0
4	0.0	0.0	0.0	1.1795217897857693	0.0
5	0.0	0.0	0.0	2.099142747925457	0.0
6	0.0	0.0	0.0	2.348801182265289	0.0
7	0.0	0.0	0.0	2.707467987935919	0.0
8	0.0	0.0	0.0	3.2259893515648006	0.0
9	0.0	0.0	0.0	3.3863926093787438	0.0
10	0.0	0.0	0.0	4.535553007149559	0.0
11	0.0	0.0	0.0	5.270262113921064	0.0
12	0.0	0.0	0.0	6.909594382776677	0.0
13	0.0	0.0	0.0	7.173701913316397	0.0
14	0.0	0.0	0.0	7.285453783925655	0.0
15	0.0	0.0	0.0	7.58522680508535	0.0
16	0.0	0.0	0.0	7.928347114844708	0.0
17	0.0	0.0	0.0	8.40187509030685	0.0
18	0.0	0.0	0.0	8.879975564566427	0.0
19	0.0	0.0	0.0	9.679979954165272	0.0
20	0.0	0.0	0.0	10.087663946944382	0.0
21	0.0	0.0	0.0	10.592284914229067	0.0
22	1.8289995189731264E-4	0.0	0.0	11.191282256692766	0.0
23	1.8289995189731264E-4	0.0	0.0	11.77765950247555	0.0
24	1.8289995189731264E-4	0.0	0.0	12.234909382218833	0.0
25	1.8289995189731264E-4	0.0	0.0	12.644422374516916	0.0
26	1.8289995189731264E-4	0.0	0.0	13.0061984793698	0.0
27	1.8289995189731264E-4	0.0	0.0	13.375290582298577	0.0
28	1.8289995189731264E-4	0.0	0.0	13.769074178733492	0.0
29	1.8289995189731264E-4	0.0	0.0	14.207119563527554	0.0
30	1.8289995189731264E-4	0.0	0.0	14.685037137835232	0.0
31	1.8289995189731264E-4	0.0	0.0	15.160028312912553	0.0
32	1.8289995189731264E-4	0.0	0.0	15.600451397081283	0.0
33	1.8289995189731264E-4	0.0	0.0	16.05477487759421	0.0
34	1.8289995189731264E-4	0.0	0.0	16.490991262869297	0.0
35	1.8289995189731264E-4	0.0	0.0	16.9807973340503	0.0
36	1.8289995189731264E-4	0.0	0.0	17.47224950479838	0.0
37	1.8289995189731264E-4	0.0	0.0	17.937912782328937	0.0
38	3.657999037946253E-4	0.0	0.0	18.422963454760612	0.0
39	3.657999037946253E-4	0.0	0.0	18.927950221949093	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCG	100	0.0	45.000004	40
TGTTCCG	35	1.2103737E-7	45.000004	1
CGATCGA	100	0.0	45.000004	41
CGACGGT	20	7.030038E-4	45.0	28
CGTTGAT	20	7.030038E-4	45.0	25
CACGCTA	20	7.030038E-4	45.0	14
CGGTCTA	20	7.030038E-4	45.0	31
ACCCGTT	20	7.030038E-4	45.0	10
TCGCGGA	20	7.030038E-4	45.0	3
ACGACGG	20	7.030038E-4	45.0	27
TGCGACC	30	2.163406E-6	44.999996	32
TTTCGCG	155	0.0	42.096775	1
CGATGAA	1145	0.0	41.65939	19
TCGGCGT	65	0.0	41.53846	4
ACCCCCG	170	0.0	41.02941	36
GGCCGAT	160	0.0	40.78125	8
CCGATCA	50	1.0786607E-9	40.500004	40
CCGATGA	1170	0.0	40.384617	18
ACGGGAT	45	1.9250365E-8	40.0	5
GTGCTCG	45	1.9250365E-8	40.0	1
>>END_MODULE