##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934095.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	515086
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.1109717600556	31.0	30.0	31.0	26.0	34.0
2	30.515972090097577	31.0	30.0	33.0	27.0	34.0
3	30.537919881340205	31.0	30.0	34.0	27.0	34.0
4	34.109003156754405	35.0	33.0	37.0	30.0	37.0
5	34.49290021472142	35.0	35.0	37.0	32.0	37.0
6	34.13675192103843	35.0	35.0	37.0	30.0	37.0
7	34.523436862970456	35.0	35.0	37.0	32.0	37.0
8	34.815308511588356	35.0	35.0	37.0	32.0	37.0
9	36.09961443331793	38.0	35.0	39.0	31.0	39.0
10	35.36134548405509	37.0	34.0	39.0	30.0	39.0
11	35.49455624885941	37.0	35.0	39.0	30.0	39.0
12	35.610783441988325	37.0	35.0	39.0	30.0	39.0
13	35.69151753299449	37.0	35.0	39.0	30.0	39.0
14	36.45289718610096	38.0	35.0	40.0	30.0	41.0
15	36.71763550164439	38.0	35.0	40.0	31.0	41.0
16	36.80142927588791	38.0	35.0	40.0	31.0	41.0
17	36.55165156886423	38.0	35.0	40.0	31.0	41.0
18	36.642778099191204	38.0	35.0	40.0	31.0	41.0
19	36.61834140318316	38.0	35.0	40.0	31.0	41.0
20	36.65969372104853	38.0	35.0	40.0	31.0	41.0
21	36.301382681726935	38.0	35.0	40.0	30.0	41.0
22	36.471744524215374	38.0	35.0	40.0	30.0	41.0
23	36.5685710735683	38.0	35.0	40.0	31.0	41.0
24	36.45737022555457	38.0	35.0	40.0	30.0	41.0
25	35.9175244522274	38.0	34.0	40.0	29.0	41.0
26	36.046889257327905	38.0	34.0	40.0	30.0	41.0
27	36.12818636111251	38.0	35.0	40.0	30.0	41.0
28	35.99478533681754	38.0	34.0	40.0	30.0	41.0
29	36.10770240309385	38.0	35.0	40.0	30.0	41.0
30	35.61057570968732	38.0	34.0	40.0	28.0	41.0
31	35.749649573081	38.0	34.0	40.0	29.0	41.0
32	35.81343697945586	38.0	34.0	40.0	29.0	41.0
33	35.78247127664118	38.0	34.0	40.0	29.0	41.0
34	35.72327921939249	38.0	34.0	40.0	29.0	41.0
35	35.70054126883666	38.0	34.0	40.0	29.0	41.0
36	35.478857899457566	38.0	34.0	40.0	27.0	41.0
37	35.54430133997041	38.0	34.0	40.0	28.0	41.0
38	35.451611963827396	38.0	34.0	40.0	28.0	41.0
39	35.389830047797844	38.0	34.0	40.0	27.0	41.0
40	35.31687912309789	38.0	34.0	40.0	27.0	41.0
41	35.229447121451564	38.0	34.0	40.0	27.0	41.0
42	35.40867544448888	38.0	34.0	40.0	28.0	41.0
43	35.34975324508917	38.0	34.0	40.0	27.0	41.0
44	35.27471334883884	38.0	34.0	40.0	27.0	41.0
45	35.36469832222192	38.0	34.0	40.0	28.0	41.0
46	35.260857410218875	38.0	34.0	40.0	27.0	41.0
47	35.235494655261455	38.0	34.0	40.0	27.0	41.0
48	35.19898230586737	37.0	34.0	40.0	27.0	41.0
49	35.15864923527333	37.0	34.0	40.0	27.0	41.0
50	34.9726220475804	37.0	34.0	40.0	27.0	41.0
51	33.901927056841	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	9.0
12	15.0
13	13.0
14	20.0
15	36.0
16	68.0
17	149.0
18	292.0
19	491.0
20	783.0
21	1307.0
22	1773.0
23	2682.0
24	3844.0
25	5189.0
26	6557.0
27	7806.0
28	9510.0
29	11520.0
30	14885.0
31	18771.0
32	23899.0
33	31316.0
34	42792.0
35	48194.0
36	59986.0
37	77792.0
38	96086.0
39	49295.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	22.913066944160782	4.759011116590239	52.026651860077735	20.301270079171246
2	19.85532513017244	3.4652465801827264	55.62566251072636	21.05376577891847
3	19.654776095642283	3.4114691527240106	56.28710545423483	20.64664929739888
4	18.660379043499532	4.939369348031203	50.36712316001599	26.03312844845327
5	16.85446702104115	5.542763732658236	53.73607514085027	23.86669410545035
6	21.54572246188015	5.651677583937439	55.20786820064999	17.594731753532418
7	84.06615594289109	1.8719204171730546	9.928244992098408	4.133678647837448
8	86.89267423304071	2.003160637252808	7.248498309020242	3.8556668206862543
9	82.03290324334189	4.798033726406852	9.119448014506315	4.049615015744943
10	37.88610057349647	30.838151299006377	17.47863463576956	13.797113491727595
11	25.796857223842235	27.32786369654776	27.621406910690645	19.253872168919365
12	23.856210419230965	23.460936620292532	32.14045809825932	20.542394862217183
13	22.1929930147587	25.034654407225197	32.36372178626482	20.408630791751282
14	19.46587560135589	27.103435154517886	31.654713970094317	21.775975274031907
15	18.059314366921253	27.25603103171121	34.452693336646696	20.231961264720844
16	21.02852727505698	26.303180439771225	32.0468814916344	20.62141079353739
17	20.801769024978352	26.41578299546095	30.05595182163755	22.726496157923144
18	21.16986289668133	25.801516639939738	31.838566763608405	21.190053699770523
19	22.449066757784138	27.999207899263425	29.14057070081501	20.41115464213743
20	23.70905052748473	28.137243101152045	29.634857091825445	18.518849279537786
21	23.5519893765313	27.234481232260244	29.903355944444225	19.310173446764228
22	22.712517909630627	25.42216251266779	29.84977265932291	22.01554691837868
23	21.40807554466633	26.786594859887476	29.71833829690576	22.086991298540436
24	21.260915652920094	26.30259801275903	31.168193272579725	21.268293061741144
25	20.611315391992793	28.763740423929207	28.185196258488876	22.439747925589124
26	20.21681816240395	30.36560884978431	27.815160963411934	21.602412024399808
27	20.129842395250503	28.966230881833326	30.04915683982869	20.854769883087485
28	18.719592456405337	28.565909382122594	31.45882435166166	21.2556738098104
29	19.958026426654964	27.00209285439713	30.29765903169568	22.742221687252226
30	20.502789825388383	27.83574005117592	29.60825959160218	22.05321053183352
31	20.277778856346316	29.556423587517422	28.337986278019596	21.827811278116663
32	20.331362141467636	30.11535937688075	27.831857204428	21.72142127722361
33	21.85188492795378	28.678706080149723	27.722166783799214	21.74724220809729
34	19.607793649992427	28.72335881775082	28.3923461324905	23.276501399766254
35	19.764078231596276	28.745685186551373	27.77031408347344	23.719922498378914
36	21.123462878043668	29.627479683004392	27.386688824778776	21.862368614173167
37	19.49460866729051	29.696206070442603	29.333159899511923	21.476025362754957
38	19.904637283871043	28.232566988813517	28.649973014215103	23.212822713100337
39	21.595034615578758	26.925989058137866	28.820041701774073	22.658934624509307
40	21.213156637920658	27.58335501256101	28.877119548968523	22.32636880054981
41	19.097975871990307	27.722749210811397	29.60476502952905	23.574509887669244
42	20.714404973150113	28.22285987194371	28.40108253767332	22.66165261723285
43	20.604326267846535	28.295663248467246	28.370408048364737	22.729602435321482
44	19.98404149986604	27.43464198211561	28.372737756413493	24.20857876160486
45	20.677129644370066	27.8270036459931	27.22030884163033	24.27555786800651
46	20.535017453396133	28.520285932834515	27.758471400892276	23.186225212877073
47	18.42333124953891	28.642013178381866	29.658736599325163	23.275918972754063
48	19.541591112940363	27.689356728779273	29.76493245788082	23.004119700399546
49	20.283797268805596	26.977048492873035	29.542445339224905	23.19670889909646
50	19.852995422123684	26.538675095032676	29.02486186772694	24.5834676151167
51	19.452673922412956	26.40296960119281	27.700616984348247	26.443739492045985
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	188.0
1	400.5
2	613.0
3	1962.0
4	3311.0
5	2432.5
6	1554.0
7	1560.5
8	1567.0
9	1777.5
10	1988.0
11	2226.5
12	2465.0
13	2444.0
14	2423.0
15	2376.0
16	2329.0
17	2330.0
18	2331.0
19	2264.5
20	2198.0
21	2483.5
22	2769.0
23	3144.5
24	3520.0
25	4191.0
26	6145.0
27	7428.0
28	7834.5
29	8241.0
30	9117.5
31	9994.0
32	12081.5
33	14169.0
34	16143.0
35	18117.0
36	19385.5
37	20654.0
38	22435.0
39	24216.0
40	26475.0
41	28734.0
42	31884.0
43	35034.0
44	36038.5
45	37043.0
46	40546.0
47	44049.0
48	46178.5
49	48308.0
50	46693.5
51	45079.0
52	39129.0
53	33179.0
54	29147.0
55	25115.0
56	22566.5
57	20018.0
58	18813.5
59	17609.0
60	16143.0
61	14677.0
62	12655.0
63	10633.0
64	9088.0
65	7543.0
66	6174.0
67	4805.0
68	3895.5
69	2986.0
70	2640.5
71	2295.0
72	1800.5
73	1306.0
74	1105.5
75	636.0
76	367.0
77	302.5
78	238.0
79	188.5
80	139.0
81	102.5
82	66.0
83	40.0
84	14.0
85	8.0
86	2.0
87	1.0
88	0.0
89	2.0
90	4.0
91	2.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	515086.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.19811150552492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.80528050787836	29.714238380554114
2	9.011513075497453	7.064685887336901
3	3.0982915495668673	3.6434153290964306
4	1.7641952019627538	2.7661248097619233
5	1.2808126228662926	2.510271800439839
6	0.9576459869021442	2.2522748506445223
7	0.8267657532535386	2.268535932948706
8	0.7535813691362123	2.363117322870991
9	0.6376996849630969	2.2496961022399433
>10	5.739695899994595	38.7764257002846
>50	0.09001326359904431	2.3800680563530174
>100	0.03000442119966337	2.1648387097966073
>500	0.0030004421199663366	0.8442557337100677
>1k	0.0015002210599831683	1.0020513839623557
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2101	0.40789305086917527	No Hit
CCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	1682	0.3265474115002155	TruSeq Adapter, Index 13 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGCT	1329	0.25801516639939737	TruSeq Adapter, Index 16 (95% over 22bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCC	877	0.17026282989636682	No Hit
TCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	844	0.163856132762296	TruSeq Adapter, Index 13 (95% over 21bp)
CGCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTG	779	0.15123688083155046	No Hit
GCTGTCTCTTATACACATCTGACGCAATCGCTGTCGTATGCCGTCTTCTGC	660	0.1281339426814163	TruSeq Adapter, Index 13 (95% over 21bp)
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	632	0.12269795723432592	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2811181045495315	0.0
2	0.0	0.0	0.0	0.9676054095820892	0.0
3	0.0	0.0	0.0	1.339970412707781	0.0
4	0.0	0.0	0.0	1.8212492671126763	0.0
5	0.0	0.0	0.0	2.582675514380123	0.0
6	0.0	0.0	0.0	3.1437468694548096	0.0
7	0.0	0.0	0.0	3.6506525123959883	0.0
8	0.0	0.0	0.0	4.362184178952641	0.0
9	0.0	0.0	0.0	4.6617458055548004	0.0
10	0.0	0.0	0.0	5.254462361625049	0.0
11	0.0	0.0	0.0	6.522600109496278	0.0
12	0.0	0.0	0.0	7.611738622288317	0.0
13	0.0	0.0	0.0	7.996334592669962	0.0
14	0.0	0.0	0.0	8.15902587140788	0.0
15	0.0	0.0	0.0	8.337442679474885	0.0
16	0.0	0.0	0.0	8.765914818108044	0.0
17	0.0	0.0	0.0	9.47628163064032	0.0
18	0.0	0.0	0.0	10.221982348578685	0.0
19	0.0	0.0	0.0	10.744807663186341	0.0
20	0.0	0.0	0.0	11.288406207895381	0.0
21	0.0	0.0	0.0	12.09526176211351	0.0
22	0.0	0.0	0.0	12.973561696493402	0.0
23	0.0	0.0	0.0	13.88020641213312	0.0
24	0.0	0.0	0.0	14.567664428852657	0.0
25	0.0	0.0	0.0	15.169311532443126	0.0
26	0.0	0.0	0.0	15.68340044186796	0.0
27	0.0	0.0	0.0	16.20544918712603	0.0
28	0.0	0.0	0.0	16.77448037803396	0.0
29	0.0	0.0	0.0	17.366420364754624	0.0
30	0.0	0.0	0.0	18.04009427551904	0.0
31	0.0	0.0	0.0	18.66775645232058	0.0
32	0.0	0.0	0.0	19.257366730992494	0.0
33	0.0	0.0	0.0	19.82484478320125	0.0
34	0.0	0.0	0.0	20.404359660328566	0.0
35	0.0	0.0	0.0	21.0374578225772	0.0
36	0.0	0.0	0.0	21.654248028484563	0.0
37	0.0	0.0	0.0	22.28385162866007	0.0
38	0.0	0.0	0.0	22.91753221792089	0.0
39	0.0	0.0	0.0	23.559172643014954	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.0296554E-4	45.000004	15
GTTACGG	25	3.8877966E-5	45.000004	2
CGGGCTA	25	3.8877966E-5	45.000004	6
CGTAATA	20	7.0296554E-4	45.000004	40
TCGTTGA	75	0.0	42.0	24
CGTTGAT	75	0.0	42.0	25
GCTTGCG	70	0.0	41.785713	1
TACGAAT	60	3.6379788E-12	41.249996	12
GCTACGA	60	3.6379788E-12	41.249996	10
TTTACGG	55	6.002665E-11	40.909092	2
CGTTTTT	1430	0.0	40.751747	1
CTAGCGG	50	1.0786607E-9	40.500004	2
CCTTGCG	45	1.9246727E-8	40.0	1
TTTGGGC	1165	0.0	39.978542	4
TTATCGG	35	6.2415766E-6	38.571426	2
GGCGATT	550	0.0	38.454544	8
TCTCTCG	65	9.094947E-12	38.07692	1
CTACGAA	65	9.094947E-12	38.07692	11
CGAATAT	65	9.094947E-12	38.07692	14
TAGCGGG	125	0.0	37.800003	3
>>END_MODULE