SRR2934094.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934094.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	359345
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	10111	2.8137305375057395	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG	9177	2.5538131878835104	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC	5129	1.4273191501203577	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGTAGGT	5056	1.4070044108029887	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTA	2401	0.6681601246712768	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGGTCGAATGC	2057	0.572430394189428	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTC	1711	0.47614409550710324	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1625	0.452211662886641	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCT	1440	0.4007291043426234	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	1263	0.3514728186005093	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCAGTGAAGGT	1013	0.281901793541026	Illumina Single End PCR Primer 1 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCACCGGTCGAATGC	1013	0.281901793541026	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT	875	0.2434985877081913	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGAAGGACGAA	873	0.24294201950771543	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCAGGGAAGGT	858	0.23876775800414646	Illumina Single End PCR Primer 1 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	825	0.22958438269629466	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCGCCGGTCGAATGC	759	0.21121763208059108	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	742	0.20648680237654624	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCTGTGAAGGT	728	0.20259082497321515	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGT	655	0.18227608565584605	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATCGAATGC	606	0.16864016474418736	No Hit
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	594	0.16530075554133214	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTGTAGG	558	0.15528252793276656	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTAT	507	0.14109003882063198	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATG	451	0.12550612920730772	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	411	0.11437476519779043	Illumina Single End Adapter 2 (95% over 21bp)
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	409	0.11381819699731457	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	406	0.11298334469660076	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG	393	0.10936565139350762	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACAGGTCGAATGC	392	0.1090873672932697	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACCTCTGACGCTGTGTAGGT	376	0.10463482168946278	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCGCTCTATGTT	369	0.10268683298779724	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCGCCGGTCGAATGC	364	0.10129541248660758	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCA	20	7.0268055E-4	45.000004	18
GTCATAC	35	1.2088094E-7	45.000004	39
ACCGCGT	80	0.0	45.000004	39
CGACCCA	20	7.0268055E-4	45.000004	34
CGTTCAT	20	7.0268055E-4	45.000004	17
CTCGCCC	20	7.0268055E-4	45.000004	17
AAGGGCG	20	7.0268055E-4	45.000004	14
CCGGTCA	40	6.7921064E-9	45.000004	40
CCGGTAA	20	7.0268055E-4	45.000004	40
ACCCGTG	40	6.7921064E-9	45.000004	39
CGGTAGA	20	7.0268055E-4	45.000004	41
CGGTAAA	20	7.0268055E-4	45.000004	41
AGCTACG	20	7.0268055E-4	45.000004	9
AAGGCGT	20	7.0268055E-4	45.000004	14
CGATCGA	210	0.0	45.000004	41
GCGTCAT	35	1.2088094E-7	45.000004	37
CGTCATA	35	1.2088094E-7	45.000004	38
TCGCGCA	20	7.0268055E-4	45.000004	34
TGACTAT	20	7.0268055E-4	45.000004	23
CGGACGT	35	1.2088094E-7	45.000004	44

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content