##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934094.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	359345
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.158454966675478	30.0	28.0	31.0	25.0	33.0
2	27.390727573780072	31.0	25.0	31.0	16.0	34.0
3	27.63591534597671	31.0	25.0	31.0	16.0	34.0
4	33.09166121693637	35.0	32.0	35.0	28.0	37.0
5	34.28985237028483	35.0	35.0	37.0	32.0	37.0
6	32.614582086852465	35.0	32.0	37.0	26.0	37.0
7	34.22214028301493	35.0	33.0	35.0	32.0	37.0
8	32.341179089732705	35.0	31.0	37.0	25.0	37.0
9	35.15206278089301	37.0	34.0	39.0	30.0	39.0
10	35.030544462842116	37.0	34.0	39.0	30.0	39.0
11	35.156050592049425	37.0	35.0	39.0	30.0	39.0
12	35.168075248020706	37.0	34.0	39.0	30.0	39.0
13	34.78414058912744	37.0	33.0	39.0	27.0	39.0
14	35.63201658573237	38.0	34.0	40.0	27.0	41.0
15	35.74090358847347	38.0	33.0	40.0	27.0	41.0
16	36.29872963308242	38.0	35.0	40.0	30.0	41.0
17	35.43346644589462	37.0	33.0	40.0	27.0	41.0
18	36.02129708219121	38.0	34.0	40.0	30.0	41.0
19	35.541407282694905	38.0	34.0	40.0	27.0	41.0
20	34.653711614186925	37.0	32.0	40.0	25.0	41.0
21	35.36096787210063	37.0	33.0	40.0	27.0	41.0
22	35.69448023487178	38.0	34.0	40.0	29.0	41.0
23	34.67388442861317	37.0	32.0	40.0	25.0	41.0
24	34.991935326775106	37.0	33.0	40.0	26.0	41.0
25	35.35188746190986	37.0	34.0	40.0	27.0	41.0
26	35.24817932627419	37.0	33.0	40.0	27.0	41.0
27	34.30973855208783	37.0	32.0	40.0	24.0	41.0
28	34.19704462285547	37.0	32.0	40.0	24.0	41.0
29	34.60906371314475	37.0	33.0	40.0	24.0	41.0
30	34.29021135677413	37.0	32.0	40.0	24.0	41.0
31	34.39883398962	37.0	33.0	40.0	24.0	41.0
32	33.43334956657252	37.0	31.0	40.0	21.0	41.0
33	33.060309730203564	36.0	30.0	40.0	18.0	41.0
34	32.74909627238448	36.0	30.0	40.0	15.0	41.0
35	32.958730467934714	37.0	31.0	40.0	14.0	41.0
36	32.18052567866535	36.0	30.0	40.0	12.0	41.0
37	31.687923861470175	36.0	30.0	40.0	9.0	41.0
38	32.02823192196914	35.0	29.0	39.0	12.0	41.0
39	31.753493161168237	36.0	30.0	39.0	10.0	41.0
40	32.47294382835437	35.0	30.0	39.0	16.0	41.0
41	32.163430686387734	35.0	29.0	39.0	16.0	41.0
42	32.134503054168	36.0	30.0	39.0	14.0	41.0
43	31.11000013914205	36.0	29.0	39.0	7.0	41.0
44	31.681117032378356	36.0	30.0	39.0	11.0	41.0
45	31.506872225855375	36.0	30.0	40.0	7.0	41.0
46	31.419260042577466	36.0	30.0	40.0	7.0	41.0
47	31.209088758713772	35.0	29.0	39.0	10.0	41.0
48	31.547128803795797	35.0	29.0	39.0	11.0	41.0
49	31.94855918407102	36.0	30.0	39.0	10.0	41.0
50	31.016783314085348	35.0	29.0	39.0	7.0	41.0
51	30.309443570941575	35.0	26.0	39.0	7.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	5.0
12	3.0
13	6.0
14	11.0
15	18.0
16	29.0
17	72.0
18	303.0
19	1557.0
20	4213.0
21	7091.0
22	9421.0
23	10923.0
24	11563.0
25	10242.0
26	7946.0
27	6751.0
28	7027.0
29	8830.0
30	11425.0
31	14544.0
32	18083.0
33	22417.0
34	29098.0
35	30415.0
36	35057.0
37	41830.0
38	47378.0
39	23085.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.500591353713006	3.3277212706452017	37.66463983080327	11.507047544838525
2	15.046821299865032	31.755555246350998	38.57407227038083	14.623551183403135
3	15.100530131210954	31.46920090720617	37.94236736284072	15.487901598742157
4	13.78953373499005	3.2364440857671593	63.49385687848724	19.48016530075554
5	39.36439911505656	4.059330170170727	37.87557917878362	18.70069153598909
6	14.954987546786514	30.989995686596444	40.68903143218912	13.365985334427918
7	61.411735240507035	1.7776788323199153	33.68990802710487	3.1206779000681797
8	62.053458375655715	26.9276043913231	6.427249579095299	4.591687653925893
9	58.95782604460894	3.290431201213319	6.722230725347507	31.02951202883023
10	52.0321696419875	17.519375530479067	15.967941671652591	14.480513155880839
11	45.1404638995951	19.915958201728145	19.73451696837301	15.209060930303748
12	21.595402746664067	16.626083568715302	23.974175235497917	37.80433844912271
13	36.33054585426262	19.692496069237084	29.44106638467211	14.535891691828187
14	18.29968414754623	40.18923318816179	22.45168292309619	19.059399741195787
15	13.76281846136721	19.72922957046849	33.348453436112926	33.15949853205137
16	15.606450625443516	19.646579192697825	24.846039321543365	39.9009308603153
17	16.22284990747054	37.46177072172981	27.586859424786763	18.728519946012884
18	16.7691215962376	19.890634348606493	25.853149480304445	37.48709457485147
19	18.11657320958967	24.463398683716207	22.760578274360295	34.65944983233383
20	35.5329836229807	20.872698938346158	29.345336654190262	14.248980784482878
21	20.972881214431812	41.93156993975149	21.408674115404416	15.686874730412278
22	17.724749196454663	18.404318969235693	41.96051148617624	21.910420348133407
23	31.742754177740057	26.6693567463023	23.02244361268419	18.565445463273456
24	18.803656653077127	36.14604349580487	23.439313194840615	21.610986656277394
25	16.0633931180342	43.02800929468894	20.656193908361047	20.252403678915805
26	15.913119703905718	21.91542946193769	41.286785679500206	20.88466515465639
27	33.83211120232645	24.419151511778374	22.25744062113011	19.49129666476506
28	14.63384769511194	38.766644867745484	29.039780712129016	17.559726725013565
29	19.83831693776176	19.480721868956017	40.76945553715788	19.911505656124337
30	18.716832013802893	40.43885402607522	25.017184043189694	15.827129916932195
31	19.93098554314099	21.67610513573307	21.867008028496294	36.52590129262965
32	37.791815664612	25.61827769970363	21.010449567963935	15.579457067720437
33	34.210299294549806	20.190624608662986	21.67471371523188	23.92436238155533
34	20.058996229250443	24.631482280259917	24.11721326302022	31.19230822746942
35	37.27699007917183	20.552115654872058	22.029804227135482	20.141090038820632
36	18.873227678136608	35.59587582963447	26.89949769719907	18.631398795029845
37	25.133785081189387	22.09603584299211	24.370730078336976	28.39944899748153
38	19.563372246726686	20.51566043774089	26.209353128609	33.71161418692343
39	22.89025866507117	36.01441511639232	22.08685246768426	19.008473750852247
40	19.62849072618236	20.842365971420225	26.26445338045611	33.26468992194131
41	19.737021525275154	25.316896019145947	22.181747345865393	32.76433510971351
42	33.540191181176866	19.81410622104106	24.684634543405362	21.961068054376714
43	26.51184794556763	27.015820451098527	24.11025616051427	22.362075442819574
44	24.447258205902404	21.9763736798898	33.5140324757545	20.062335638453295
45	23.763514171617807	19.195758950312374	28.878097649890773	28.16262922817905
46	32.238099876163574	28.001781018241523	22.867995937052136	16.892123168542767
47	16.10791857407227	34.09787251805368	32.539759840821496	17.254449067052555
48	20.860732722035927	30.79297054362799	26.52965812798286	21.816638606353226
49	28.52217228568646	18.857365484423045	32.17687737411123	20.443584855779264
50	27.564318412667493	24.518777219663555	25.541193003937718	22.37571136373123
51	20.155839096133242	20.733278604126955	31.223197762595834	27.88768453714397
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	28.0
1	136.0
2	244.0
3	558.0
4	872.0
5	693.0
6	514.0
7	501.0
8	488.0
9	506.5
10	525.0
11	530.0
12	535.0
13	531.0
14	527.0
15	582.5
16	638.0
17	632.5
18	627.0
19	717.0
20	807.0
21	1003.5
22	1200.0
23	1214.5
24	1229.0
25	1556.5
26	2275.5
27	2667.0
28	3070.0
29	3473.0
30	4013.0
31	4553.0
32	5523.5
33	6494.0
34	7297.5
35	8101.0
36	8916.5
37	9732.0
38	10247.0
39	10762.0
40	12557.5
41	14353.0
42	16019.5
43	17686.0
44	18953.5
45	20221.0
46	38071.5
47	55922.0
48	46171.0
49	36420.0
50	34156.0
51	31892.0
52	29773.5
53	27655.0
54	27982.0
55	28309.0
56	26168.0
57	24027.0
58	19969.0
59	15911.0
60	13084.0
61	10257.0
62	8693.5
63	7130.0
64	6091.0
65	5052.0
66	3949.0
67	2846.0
68	2500.5
69	2155.0
70	1759.5
71	1364.0
72	1158.5
73	953.0
74	797.0
75	501.5
76	362.0
77	246.0
78	130.0
79	131.0
80	132.0
81	76.5
82	21.0
83	13.0
84	5.0
85	3.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	359345.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.2415596016344
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.86807718078381	21.6001778154002
2	9.756361002407473	5.705824234939193
3	3.5900202015521416	3.1493336908427545
4	1.9623478145610889	2.2952844231450036
5	1.2670680085542663	1.8525522345731893
6	0.984653771734609	1.727568717189101
7	0.7990875862884066	1.635659709696503
8	0.6664040726073684	1.5589355526336208
9	0.5596595730218061	1.4728786885028167
>10	6.2141164573228505	34.52206960378972
>50	0.20949822686156527	4.115833733506401
>100	0.09876344980616648	5.61000769218666
>500	0.011971327249232302	2.502930996774039
>1k	0.007980884832821535	3.6531707310724117
>5k	0.0029928318123080755	5.64822260600687
>10k+	9.976106041026918E-4	2.949549569741528
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	10111	2.8137305375057395	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG	9177	2.5538131878835104	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTC	5129	1.4273191501203577	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGTAGGT	5056	1.4070044108029887	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTA	2401	0.6681601246712768	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGGTCGAATGC	2057	0.572430394189428	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTC	1711	0.47614409550710324	No Hit
GCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	1625	0.452211662886641	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCT	1440	0.4007291043426234	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCT	1263	0.3514728186005093	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCAGTGAAGGT	1013	0.281901793541026	Illumina Single End PCR Primer 1 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCACCGGTCGAATGC	1013	0.281901793541026	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATGAATGAT	875	0.2434985877081913	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGAAGGACGAA	873	0.24294201950771543	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCAGGGAAGGT	858	0.23876775800414646	Illumina Single End PCR Primer 1 (95% over 21bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	825	0.22958438269629466	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGAGCGCCGGTCGAATGC	759	0.21121763208059108	No Hit
CCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	742	0.20648680237654624	Illumina Single End Adapter 2 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCTGTGAAGGT	728	0.20259082497321515	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGT	655	0.18227608565584605	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGATCGAATGC	606	0.16864016474418736	No Hit
CTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGCT	594	0.16530075554133214	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCGACCACCCTGTCTCTTATACACATCTGACGCTGTGTAGG	558	0.15528252793276656	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTAT	507	0.14109003882063198	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATG	451	0.12550612920730772	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCGTCTTCTGC	411	0.11437476519779043	Illumina Single End Adapter 2 (95% over 21bp)
GAATGCTACGGCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGC	409	0.11381819699731457	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	406	0.11298334469660076	No Hit
GAATGATCCCTGTCTCTTATACACATCTGACGCTGTGTAGGTCGTATGCCG	393	0.10936565139350762	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACAGGTCGAATGC	392	0.1090873672932697	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACCTCTGACGCTGTGTAGGT	376	0.10463482168946278	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCGCTCTATGTT	369	0.10268683298779724	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCGCCGGTCGAATGC	364	0.10129541248660758	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18199780155560813	0.0
2	0.0	0.0	0.0	1.0510790465986726	0.0
3	0.0	0.0	0.0	1.2756543154906845	0.0
4	0.0	0.0	0.0	1.9335179284531578	0.0
5	0.0	0.0	0.0	3.9652701442903058	0.0
6	0.0	0.0	0.0	4.333161724804853	0.0
7	0.0	0.0	0.0	4.994921315170657	0.0
8	0.0	0.0	0.0	5.898509788643226	0.0
9	0.0	0.0	0.0	6.061862555482892	0.0
10	0.0	0.0	0.0	9.30136776635267	0.0
11	0.0	0.0	0.0	10.050230280092947	0.0
12	0.0	0.0	0.0	14.09008056324702	0.0
13	0.0	0.0	0.0	14.461311552964421	0.0
14	0.0	0.0	0.0	14.70258386787071	0.0
15	0.0	0.0	0.0	15.612851159748988	0.0
16	0.0	0.0	0.0	16.143538938902726	0.0
17	0.0	0.0	0.0	16.700941991679304	0.0
18	0.0	0.0	0.0	17.243595987143273	0.0
19	0.0	0.0	0.0	19.450945470230558	0.0
20	0.0	0.0	0.0	19.99610402259667	0.0
21	0.0	0.0	0.0	20.58133548539704	0.0
22	0.0	0.0	0.0	21.253948155672127	0.0
23	0.0	0.0	0.0	21.96301604307838	0.0
24	0.0	0.0	0.0	22.467266832709512	0.0
25	0.0	0.0	0.0	22.907233995185685	0.0
26	0.0	0.0	0.0	23.309911088229974	0.0
27	0.0	0.0	0.0	23.753495944009238	0.0
28	0.0	0.0	0.0	24.24605880143038	0.0
29	0.0	0.0	0.0	24.711628101128444	0.0
30	0.0	0.0	0.0	25.171631718821747	0.0
31	0.0	0.0	0.0	25.673934519751214	0.0
32	0.0	0.0	0.0	26.185420695988533	0.0
33	0.0	0.0	0.0	26.67325272370563	0.0
34	0.0	0.0	0.0	27.143831137207975	0.0
35	0.0	0.0	0.0	27.62776718752174	0.0
36	0.0	0.0	0.0	28.116990635740027	0.0
37	0.0	0.0	0.0	28.612892902364024	0.0
38	0.0	0.0	0.0	29.12799677190444	0.0
39	0.0	0.0	0.0	29.62417732262867	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCCA	20	7.0268055E-4	45.000004	18
GTCATAC	35	1.2088094E-7	45.000004	39
ACCGCGT	80	0.0	45.000004	39
CGACCCA	20	7.0268055E-4	45.000004	34
CGTTCAT	20	7.0268055E-4	45.000004	17
CTCGCCC	20	7.0268055E-4	45.000004	17
AAGGGCG	20	7.0268055E-4	45.000004	14
CCGGTCA	40	6.7921064E-9	45.000004	40
CCGGTAA	20	7.0268055E-4	45.000004	40
ACCCGTG	40	6.7921064E-9	45.000004	39
CGGTAGA	20	7.0268055E-4	45.000004	41
CGGTAAA	20	7.0268055E-4	45.000004	41
AGCTACG	20	7.0268055E-4	45.000004	9
AAGGCGT	20	7.0268055E-4	45.000004	14
CGATCGA	210	0.0	45.000004	41
GCGTCAT	35	1.2088094E-7	45.000004	37
CGTCATA	35	1.2088094E-7	45.000004	38
TCGCGCA	20	7.0268055E-4	45.000004	34
TGACTAT	20	7.0268055E-4	45.000004	23
CGGACGT	35	1.2088094E-7	45.000004	44
>>END_MODULE