SRR2934093.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934093.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	230284
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	713	0.30961768946170815	RNA PCR Primer, Index 17 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGCT	529	0.22971635024578346	RNA PCR Primer, Index 17 (96% over 25bp)
GCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	518	0.22493963974917927	Illumina PCR Primer Index 9 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCGCGAGATATCGTATGCCGTCTTCTGC	483	0.20974101544180232	Illumina PCR Primer Index 9 (95% over 23bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTTG	20	7.0215255E-4	45.000004	16
CGGGTGA	30	2.157918E-6	45.000004	6
AACGTCC	30	2.157918E-6	45.000004	21
CGGGTCC	30	2.157918E-6	45.000004	6
GATAATA	20	7.0215255E-4	45.000004	9
CAACGTC	30	2.157918E-6	45.000004	20
TTTCGCG	20	7.0215255E-4	45.000004	1
CGCGCGG	20	7.0215255E-4	45.000004	2
ATGCTAC	20	7.0215255E-4	45.000004	41
TTCGTAC	20	7.0215255E-4	45.000004	33
GCGTTGA	20	7.0215255E-4	45.000004	17
AGAACGA	20	7.0215255E-4	45.000004	18
AGACGGT	20	7.0215255E-4	45.000004	14
CTACGAA	30	2.157918E-6	45.000004	11
TACGAAT	30	2.157918E-6	45.000004	12
GCGGGTG	35	1.206281E-7	45.000004	5
GCGGGTC	30	2.157918E-6	45.000004	5
GCCTCGA	30	2.157918E-6	45.000004	9
CGAATAT	30	2.157918E-6	45.000004	14
GTATCCC	20	7.0215255E-4	45.000004	38