##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934092.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	348608
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.850757871305305	31.0	30.0	31.0	25.0	34.0
2	29.922176197907106	31.0	30.0	33.0	25.0	34.0
3	29.932069832017625	31.0	30.0	33.0	25.0	34.0
4	33.82869584174775	35.0	33.0	37.0	28.0	37.0
5	34.35145206076739	35.0	35.0	37.0	32.0	37.0
6	33.7356744538278	35.0	33.0	37.0	28.0	37.0
7	34.30240843583624	35.0	35.0	37.0	32.0	37.0
8	34.16420449329907	35.0	35.0	37.0	30.0	37.0
9	35.68526539838443	37.0	35.0	39.0	30.0	39.0
10	35.19042592252616	37.0	34.0	39.0	30.0	39.0
11	35.36029867358179	37.0	35.0	39.0	30.0	39.0
12	35.374890995043145	37.0	35.0	39.0	30.0	39.0
13	35.304293074169266	37.0	34.0	39.0	30.0	39.0
14	35.99150048191665	38.0	34.0	40.0	29.0	41.0
15	36.26159755369928	38.0	35.0	40.0	30.0	41.0
16	36.492059849458414	38.0	35.0	40.0	31.0	41.0
17	36.21026769322563	38.0	34.0	40.0	30.0	41.0
18	36.32989489627318	38.0	35.0	40.0	30.0	41.0
19	36.315285363502845	38.0	35.0	40.0	30.0	41.0
20	36.27361391591702	38.0	34.0	40.0	30.0	41.0
21	36.01381207545438	38.0	34.0	40.0	29.0	41.0
22	36.2201986185056	38.0	34.0	40.0	30.0	41.0
23	36.19872751055627	38.0	34.0	40.0	30.0	41.0
24	36.12201957499541	38.0	34.0	40.0	30.0	41.0
25	35.68915802276482	38.0	34.0	40.0	29.0	41.0
26	35.77444579585093	38.0	34.0	40.0	29.0	41.0
27	35.72459897650083	38.0	34.0	40.0	29.0	41.0
28	35.60061444373049	38.0	34.0	40.0	28.0	41.0
29	35.75218583624013	38.0	34.0	40.0	29.0	41.0
30	35.37029557554617	38.0	34.0	40.0	27.0	41.0
31	35.53855620066091	38.0	34.0	40.0	28.0	41.0
32	35.5018301358546	38.0	34.0	40.0	28.0	41.0
33	35.52316068478061	38.0	34.0	40.0	28.0	41.0
34	35.495539402423354	38.0	34.0	40.0	28.0	41.0
35	35.48608465669176	38.0	34.0	40.0	28.0	41.0
36	35.25233213236644	38.0	34.0	40.0	27.0	41.0
37	35.41934780613182	38.0	34.0	40.0	27.0	41.0
38	35.3127266155682	38.0	34.0	40.0	27.0	41.0
39	35.22001503120984	38.0	34.0	40.0	27.0	41.0
40	35.17379406095098	37.0	34.0	40.0	27.0	41.0
41	34.951885785753625	37.0	33.0	40.0	26.0	41.0
42	35.12889836148339	37.0	34.0	40.0	27.0	41.0
43	35.11248450982192	37.0	34.0	40.0	27.0	41.0
44	35.16033768588213	37.0	34.0	40.0	27.0	41.0
45	35.19912910776574	37.0	34.0	40.0	27.0	41.0
46	35.07059505232238	37.0	34.0	40.0	27.0	41.0
47	34.948219776941436	37.0	33.0	40.0	27.0	41.0
48	34.972129153662564	37.0	34.0	40.0	27.0	41.0
49	34.98478233431246	37.0	34.0	40.0	27.0	41.0
50	34.78811731228199	37.0	33.0	40.0	26.0	41.0
51	33.718316848724065	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	4.0
11	6.0
12	17.0
13	7.0
14	15.0
15	25.0
16	40.0
17	88.0
18	196.0
19	352.0
20	700.0
21	1193.0
22	1807.0
23	2437.0
24	3050.0
25	3664.0
26	4258.0
27	5401.0
28	6719.0
29	8650.0
30	11097.0
31	14266.0
32	18000.0
33	23315.0
34	31973.0
35	33069.0
36	39727.0
37	49972.0
38	59621.0
39	28936.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.620111988250414	4.559849458417477	50.04360198274279	16.776436570589315
2	19.9157793280705	8.787807508720396	50.80434183954471	20.4920713236644
3	22.243608867266385	9.11224068294474	47.60016981824858	21.043980631540297
4	19.43529695245089	5.149623646043694	48.26137093813108	27.153708463374333
5	22.133169634661282	5.7353818615751795	46.4298008077841	25.70164769597944
6	22.138046172204884	10.134592436203414	50.01032678538645	17.71703460620525
7	81.1493138424821	2.5217436203414727	11.86117358178814	4.467768955388287
8	82.09392785019277	6.895424086653204	6.067273269689737	4.943374793464293
9	76.18069579585092	5.484383605654489	8.435262529832936	9.89965806866165
10	39.9735519552047	28.868815402974114	15.934803561593538	15.222829080227648
11	32.81938452359097	24.94664494217	23.701980447953	18.53199008628603
12	28.25637965852763	21.38619882504131	27.57452496787222	22.78289654855884
13	25.847656967137873	24.395309344593354	29.85330227648247	19.903731411786303
14	20.13694464843033	29.668280704975214	27.704757205801357	22.490017440793096
15	17.981515054158255	25.07974573159537	35.26654580503029	21.67219340921608
16	19.630358454194972	23.767670277216816	32.10368092527997	24.498290343308245
17	20.11887277400404	25.47933495502111	29.416421883605654	24.985370387369194
18	21.197161281439325	23.736403065907837	29.8355172572058	25.230918395447034
19	24.039608959060033	25.80692353589132	27.449742977785935	22.703724527262715
20	28.704447402239765	23.791192399485954	29.2047227831834	18.299637415090874
21	25.262472461905638	28.190689829263814	26.76588030108317	19.780957407747383
22	23.02127317789609	23.872085551679824	28.23916834955021	24.867472920873876
23	23.26997659261979	27.709346888195334	26.785960161556822	22.234716357628052
24	22.74273682761153	25.527239765008265	27.865109234440975	23.864914172939233
25	21.620559482283827	29.04809987148889	25.966128143932437	23.365212502294842
26	22.249632825408483	26.86082935560859	27.16288782816229	23.726649990820636
27	22.468503304571325	27.447161281439325	26.727441711033595	23.356893702955755
28	19.36300945474573	28.08885625114742	30.714154580503028	21.833979713603817
29	21.578678630438773	24.19508445015605	29.41613502845603	24.810101890949145
30	21.072092436203416	26.769035707729028	27.8447425188177	24.314129337249863
31	22.683357811639436	26.3404741141913	26.271342023132	24.70482605103727
32	24.008054892601432	27.63017486689921	25.298042500458966	23.063727740040388
33	22.650082614283093	25.8054892601432	25.833601064806317	25.710827060767393
34	22.834530475491096	25.07687718009914	27.704757205801357	24.383835138608408
35	21.560033045713237	24.86546493482651	26.775633376170372	26.798868643289886
36	21.873852579401508	26.39354231687167	28.067915825224894	23.66468927850193
37	19.967986965302	26.513447769414356	28.96290389205067	24.555661373232972
38	19.955652193868183	25.965841288782816	27.172354048099873	26.90615246924913
39	21.223838810354323	24.5814783366991	28.454596566917566	25.74008628602901
40	21.930363043877364	24.137426565081697	28.760957866715625	25.171252524325315
41	18.78872544519919	25.239237194786117	30.679445107398568	25.29259225261612
42	21.445577841013403	25.844788415641638	28.12786855149624	24.581765191848724
43	21.589865981274095	25.23981090508537	28.60863778226547	24.56168533137507
44	21.371282357260878	24.424568569854966	28.445417202129615	25.75873187075454
45	21.86180466311731	23.533883330273543	26.37977326968974	28.224538736919406
46	22.030475491096016	26.47214062786855	26.97872682210391	24.518657058931524
47	18.698366073067742	26.206512759317057	31.001870295575547	24.093250872039654
48	20.25914494217	24.602418762621628	30.093113181567837	25.04532311364054
49	20.33602212226914	22.952141086836793	31.59365246924913	25.118184321644943
50	20.21898522122269	24.01895538828713	29.141901505415824	26.620157885074352
51	19.179709014136222	24.502880025702222	27.647386175876633	28.670024784284927
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	83.0
1	227.0
2	371.0
3	481.5
4	592.0
5	478.5
6	365.0
7	384.5
8	404.0
9	433.5
10	463.0
11	488.0
12	513.0
13	506.0
14	499.0
15	528.5
16	558.0
17	552.0
18	546.0
19	616.5
20	687.0
21	848.0
22	1009.0
23	1181.5
24	1354.0
25	1513.0
26	2318.0
27	2964.0
28	3525.5
29	4087.0
30	5005.5
31	5924.0
32	7080.0
33	8236.0
34	9424.5
35	10613.0
36	10573.0
37	10533.0
38	11501.0
39	12469.0
40	14558.5
41	16648.0
42	18422.0
43	20196.0
44	21981.0
45	23766.0
46	30304.0
47	36842.0
48	34849.0
49	32856.0
50	31505.5
51	30155.0
52	27493.5
53	24832.0
54	22416.5
55	20001.0
56	19010.5
57	18020.0
58	17136.0
59	16252.0
60	15469.0
61	14686.0
62	12363.0
63	10040.0
64	8636.5
65	7233.0
66	6057.5
67	4882.0
68	3921.5
69	2961.0
70	2521.0
71	2081.0
72	1756.5
73	1432.0
74	1226.0
75	733.5
76	447.0
77	321.5
78	196.0
79	138.0
80	80.0
81	58.0
82	36.0
83	19.0
84	2.0
85	1.5
86	1.0
87	1.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	348608.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.187227493847963
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.33745369189074	21.534799434289496
2	10.606830362621377	6.403816022902108
3	3.599063235845907	3.2593722199567488
4	1.8642482900549886	2.25105948947628
5	1.1471993223381522	1.7315383462105007
6	0.849870610268599	1.5393142471508157
7	0.7401384299449796	1.5639909013181983
8	0.6653256380271654	1.6067469114092465
9	0.5945037405340968	1.6151777695301708
>10	8.398861254906151	49.57577799018388
>50	0.1486259295005624	2.9653747336840603
>100	0.0389020889296774	2.296298509248035
>500	0.003989957838941272	0.8274624053781274
>1k	0.00498744729867659	2.8292710192623307
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC	2986	0.8565494767762071	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG	2462	0.7062373783734166	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC	1807	0.5183472553699284	No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	1091	0.31295896823939784	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGCTCCTATT	1050	0.3011979071048283	No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	887	0.25444051771617404	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT	635	0.18215302001101524	No Hit
TCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	628	0.1801450339636497	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTA	598	0.17153937947494033	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	490	0.14055902331558656	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT	458	0.1313796585276299	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	454	0.1302322379291353	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTC	450	0.12908481733064073	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCT	392	0.11244721865246925	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1987906186891867	0.0
2	0.0	0.0	0.0	1.0295231319992657	0.0
3	0.0	0.0	0.0	1.3229759500642555	0.0
4	0.0	0.0	0.0	1.9213557921791813	0.0
5	0.0	0.0	0.0	3.1843790159720946	0.0
6	0.0	0.0	0.0	3.6393312832751974	0.0
7	0.0	0.0	0.0	4.145056912061685	0.0
8	0.0	0.0	0.0	4.868218744262897	0.0
9	0.0	0.0	0.0	5.102292546355792	0.0
10	0.0	0.0	0.0	6.440184964200477	0.0
11	0.0	0.0	0.0	7.539413897558289	0.0
12	0.0	0.0	0.0	9.572356342941069	0.0
13	0.0	0.0	0.0	10.002352212226914	0.0
14	0.0	0.0	0.0	10.173317881402607	0.0
15	0.0	0.0	0.0	10.576923076923077	0.0
16	0.0	0.0	0.0	11.109613089774188	0.0
17	0.0	0.0	0.0	11.789172939232605	0.0
18	0.0	0.0	0.0	12.51807187442629	0.0
19	0.0	0.0	0.0	13.340198733247659	0.0
20	0.0	0.0	0.0	13.870880760051405	0.0
21	0.0	0.0	0.0	14.559906829447403	0.0
22	0.0	0.0	0.0	15.315196438406462	0.0
23	0.0	0.0	0.0	16.0750757297595	0.0
24	0.0	0.0	0.0	16.62813245823389	0.0
25	0.0	0.0	0.0	17.134144942170003	0.0
26	0.0	0.0	0.0	17.637288874609876	0.0
27	0.0	0.0	0.0	18.102281072149808	0.0
28	0.0	0.0	0.0	18.6068592803378	0.0
29	0.0	0.0	0.0	19.120329998164127	0.0
30	0.0	0.0	0.0	19.723012667523406	0.0
31	0.0	0.0	0.0	20.24795759133468	0.0
32	0.0	0.0	0.0	20.752822654672297	0.0
33	0.0	0.0	0.0	21.278914999082062	0.0
34	0.0	0.0	0.0	21.797549109601615	0.0
35	0.0	0.0	0.0	22.392773545070682	0.0
36	0.0	0.0	0.0	22.99373508353222	0.0
37	0.0	0.0	0.0	23.555684321644943	0.0
38	0.0	0.0	0.0	24.098987975032127	0.0
39	0.0	0.0	0.0	24.651184138057648	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCGATCA	35	1.2086639E-7	45.000004	40
CTTCTCG	65	0.0	45.000004	1
ATAGTTG	35	1.2086639E-7	45.000004	17
CGGTAGT	35	1.2086639E-7	45.000004	12
CGGAAAC	40	6.7921064E-9	45.0	22
CTCACTA	20	7.0265174E-4	45.0	45
CGGCGAA	25	3.8851955E-5	45.0	31
CCGATCG	45	3.8380676E-10	45.0	40
GATCCGT	20	7.0265174E-4	45.0	27
TACGGGT	20	7.0265174E-4	45.0	4
TAACGAA	20	7.0265174E-4	45.0	19
ATCCGTC	20	7.0265174E-4	45.0	28
CGGGACC	90	0.0	45.0	6
TCATCGA	20	7.0265174E-4	45.0	16
TACTAAC	20	7.0265174E-4	45.0	13
CGATCGA	45	3.8380676E-10	45.0	41
TCGACAA	20	7.0265174E-4	45.0	19
CCGTTTG	20	7.0265174E-4	45.0	1
CGTGGCA	30	2.1611359E-6	44.999996	15
GGCACGT	30	2.1611359E-6	44.999996	11
>>END_MODULE