FastQCFastQC Report
Sat 14 Jan 2017
SRR2934091.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934091.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences285321
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGC47691.671450751960073No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCG40331.413495676799114No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTC29361.029016441131217No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTATCTGT19920.698161018642161No Hit
GCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC9330.3270001156592049TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTA8920.3126303356570319No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTC8380.29370428394685283No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCT7590.26601617125973903No Hit
GAACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT6250.21905152442336875No Hit
CCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC4870.17068494783068894TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCGGGAACTGT4460.15631516782851596No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTT3850.13493573904479517No Hit
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT3840.13458525660571777TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGC3570.12512223075062823No Hit
TCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC3250.11390679270015175TruSeq Adapter, Index 13 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT3240.11355631026107438No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCTGTCGAATGC3170.11110293318753264No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGGTCGAATGC2870.10058846001521093No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGT207.024362E-445.00000445
CCGATCG406.7848305E-945.00000440
GCGCGAT207.024362E-445.00000442
CGCGCGA207.024362E-445.00000441
CGCGATA207.024362E-445.00000443
AGGCGAC406.7848305E-945.00000431
TACGAAT406.7848305E-945.00000412
CGAATAC207.024362E-445.00000445
GCGATAG207.024362E-445.0000049
TACTCCC207.024362E-445.00000425
GAACCGA207.024362E-445.0000049
AACTAGC207.024362E-445.00000444
GACGAGG207.024362E-445.00000444
ACGAGGA207.024362E-445.00000445
CGATCGA406.7848305E-945.00000441
ACAGTTC253.8834107E-545.029
GGTCGAA1050.045.042
TACGGAG551.8189894E-1245.029
GTGGCGT502.1827873E-1145.034
CGTTCAG502.1827873E-1145.038
Produced by FastQC (version 0.11.5)