##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934091.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	285321
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.649489522327485	31.0	28.0	31.0	25.0	33.0
2	29.012252866070146	31.0	28.0	33.0	16.0	34.0
3	29.18514935809141	31.0	28.0	33.0	16.0	34.0
4	33.58538628422023	35.0	32.0	37.0	28.0	37.0
5	34.321367862863234	35.0	35.0	37.0	32.0	37.0
6	33.31309297247662	35.0	33.0	37.0	28.0	37.0
7	34.295901808839865	35.0	35.0	36.0	32.0	37.0
8	33.624349416972464	35.0	35.0	37.0	28.0	37.0
9	35.635603408091235	37.0	34.0	39.0	31.0	39.0
10	35.14998895980317	37.0	34.0	39.0	30.0	39.0
11	35.300181199421004	37.0	34.0	39.0	30.0	39.0
12	35.303447695753206	37.0	34.0	39.0	30.0	39.0
13	35.16297433417099	37.0	34.0	39.0	29.0	39.0
14	35.96276825049681	38.0	34.0	40.0	28.0	41.0
15	36.16633896558613	38.0	34.0	40.0	30.0	41.0
16	36.488109883254296	38.0	35.0	40.0	31.0	41.0
17	35.99702440409223	38.0	34.0	40.0	30.0	41.0
18	36.237637608167645	38.0	35.0	40.0	30.0	41.0
19	36.0953136993071	38.0	34.0	40.0	30.0	41.0
20	35.74677293294219	38.0	34.0	40.0	27.0	41.0
21	35.783549055274584	38.0	34.0	40.0	29.0	41.0
22	35.99006382285216	38.0	34.0	40.0	30.0	41.0
23	35.683819277235116	38.0	34.0	40.0	27.0	41.0
24	35.74196781870244	38.0	34.0	40.0	28.0	41.0
25	35.559268332860185	38.0	34.0	40.0	28.0	41.0
26	35.6360730545596	38.0	34.0	40.0	29.0	41.0
27	35.26811205624542	38.0	34.0	40.0	27.0	41.0
28	35.15235822109133	38.0	33.0	40.0	27.0	41.0
29	35.33863613263657	38.0	34.0	40.0	27.0	41.0
30	34.96191657816985	37.0	33.0	40.0	25.0	41.0
31	35.17164176488937	37.0	34.0	40.0	27.0	41.0
32	34.84755766312329	38.0	33.0	40.0	25.0	41.0
33	34.71918646016241	38.0	33.0	40.0	24.0	41.0
34	34.626543437041086	38.0	33.0	40.0	24.0	41.0
35	34.72257913017268	38.0	33.0	40.0	24.0	41.0
36	34.25837214926346	37.0	33.0	40.0	23.0	41.0
37	34.17571787565584	37.0	33.0	40.0	23.0	41.0
38	34.13050564101486	37.0	33.0	40.0	22.0	41.0
39	34.185745178237845	37.0	33.0	40.0	23.0	41.0
40	34.31932104541902	37.0	33.0	40.0	23.0	41.0
41	34.40638088328584	37.0	33.0	40.0	24.0	41.0
42	34.026510491691816	37.0	33.0	40.0	22.0	41.0
43	33.89037259788098	37.0	33.0	40.0	21.0	41.0
44	34.08278745693447	37.0	33.0	40.0	22.0	41.0
45	34.006624118098564	37.0	33.0	40.0	22.0	41.0
46	34.05387966535937	37.0	33.0	40.0	22.0	41.0
47	33.76567795570603	37.0	33.0	40.0	21.0	41.0
48	33.80050189085276	37.0	33.0	40.0	22.0	41.0
49	33.8340675940432	37.0	33.0	40.0	22.0	41.0
50	33.58226348568805	36.0	33.0	40.0	20.0	41.0
51	32.67563200745827	36.0	31.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	2.0
11	9.0
12	6.0
13	6.0
14	13.0
15	21.0
16	38.0
17	66.0
18	190.0
19	549.0
20	1406.0
21	2621.0
22	3694.0
23	4283.0
24	4779.0
25	4426.0
26	4370.0
27	4448.0
28	5540.0
29	7026.0
30	9019.0
31	11668.0
32	14425.0
33	18533.0
34	24954.0
35	26156.0
36	30401.0
37	38086.0
38	45590.0
39	22994.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	34.474153672530235	4.2622169416201405	47.781270919420585	13.482358466429039
2	17.89493237441338	17.796446809032634	46.99759218564354	17.31102863091045
3	18.224385867146125	17.25950771236607	46.29207103578075	18.22403538470705
4	16.40292863126093	4.183007910388651	56.09296196214088	23.321101496209533
5	27.615562822224792	5.2323523329863555	45.21188415854424	21.940200686244616
6	17.884417901241058	17.896684786608766	48.70829697078028	15.510600341369896
7	75.4644768523873	1.91223218760624	19.4230358087908	3.2002551512156483
8	76.86114937211072	13.39228447958615	5.8145036642939	3.932062484009239
9	73.18073327935903	3.7564707820314664	6.485677535127102	16.577118403482395
10	45.094472541453314	22.983937389817083	16.300587759050334	15.621002309679275
11	35.593594582943425	23.507558153798705	22.481345572180107	18.417501691077767
12	23.790747964573235	20.73068578898854	27.328868187059484	28.149698059378736
13	27.393707438288807	23.934095282155887	31.051692654939522	17.620504624615783
14	18.98388131262683	33.847491071459864	26.55885826840646	20.60976934750684
15	16.55328559762513	23.43851311330046	34.45698003301544	25.551221256058966
16	18.721720448196944	22.167313306766765	28.91480122388468	30.196165021151618
17	19.311231910725112	29.654319170337974	28.753579301909078	22.280869617027836
18	20.098064986453853	22.580882584878083	28.82753109655441	28.49352133211365
19	20.886299991938902	26.58163962694649	25.327963942366665	27.20409643874794
20	29.129646959039118	24.161207902678036	29.546020096663057	17.16312504161979
21	22.658689686353263	33.17491527087035	25.620266296557208	18.54612874621917
22	20.904174596331853	22.828323186866722	33.629841476792805	22.637660740008624
23	25.598536385334413	27.86055004713989	25.634986558998463	20.905927008527236
24	20.71105877240021	29.182569807339803	26.8574693065004	23.24890211375959
25	18.810042022844446	34.84496409307412	24.77700554813701	21.567988335944428
26	19.71989443468935	26.100777720532314	32.375464827334824	21.80386301744351
27	26.23816683665065	26.979437195299326	25.63638848875477	21.14600747929525
28	17.56057212753355	31.610011180389808	30.427132948503615	20.402283743573026
29	21.505251979349573	23.021789493237442	33.248166100637526	22.224792426775455
30	19.98275626399739	32.57559030004802	28.477048657477017	18.964604778477575
31	22.273159003368136	25.327963942366665	24.468581001748905	27.93029605251629
32	28.26325436963981	28.66105193799265	25.06194777110693	18.013745921260615
33	26.388523803014852	25.22947837698592	24.97467764377666	23.40732017622257
34	21.663670041812555	25.34864240627223	25.355652055053778	27.63203549686143
35	26.639469229394265	24.46963244906614	26.32438551666369	22.566512804875913
36	24.41075139930114	29.84638354695238	25.010777335001627	20.73208771874485
37	21.039110335376645	27.625376330518957	27.228630209483356	24.10688312462104
38	19.093932798497132	25.99493202393094	28.313373358427874	26.59776181914405
39	19.959624423018283	31.543069034526027	25.699124845349626	22.798181697106067
40	20.1268045464582	23.751844413835645	29.766473550842736	26.35487748886342
41	19.82714206104703	24.63365823055436	25.67564252193144	29.863557186467172
42	23.44482179720385	23.913416818250322	29.812737232800952	22.829024151744875
43	25.46990933019301	26.622996554757623	24.738452479838497	23.168641635210868
44	22.791873013202675	24.65854248372885	30.068589413327445	22.48099508974103
45	21.231174711991056	23.956876640695917	28.069437580830016	26.742511066483015
46	24.7973335296035	30.65424556902576	24.716372086176623	19.832048815194113
47	18.293430907644375	28.562566372611904	32.343570925378785	20.800431794364943
48	21.494387023738177	26.169121796152407	28.246080730125016	24.090410449984404
49	21.515766452521895	23.273435884495008	32.53914012638397	22.67165753659913
50	23.240490535221735	25.400864289694763	27.603295936857087	23.75534923822642
51	20.03708104205439	23.669481040652457	29.389704928834544	26.90373298845861
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	27.0
1	103.5
2	180.0
3	366.5
4	553.0
5	445.5
6	338.0
7	319.5
8	301.0
9	338.0
10	375.0
11	385.0
12	395.0
13	435.0
14	475.0
15	526.5
16	578.0
17	566.0
18	554.0
19	608.5
20	663.0
21	754.0
22	845.0
23	951.5
24	1058.0
25	1364.0
26	2184.5
27	2699.0
28	3241.5
29	3784.0
30	4372.0
31	4960.0
32	5817.0
33	6674.0
34	7480.5
35	8287.0
36	9223.5
37	10160.0
38	11187.5
39	12215.0
40	13883.5
41	15552.0
42	16226.5
43	16901.0
44	18020.5
45	19140.0
46	27800.0
47	36460.0
48	31686.5
49	26913.0
50	25677.0
51	24441.0
52	22109.5
53	19778.0
54	18728.0
55	17678.0
56	16372.0
57	15066.0
58	13043.5
59	11021.0
60	9652.5
61	8284.0
62	7213.5
63	6143.0
64	5304.5
65	4466.0
66	3531.0
67	2596.0
68	2084.0
69	1572.0
70	1306.5
71	1041.0
72	830.0
73	619.0
74	505.0
75	306.0
76	221.0
77	198.5
78	176.0
79	104.0
80	32.0
81	27.0
82	22.0
83	17.5
84	13.0
85	7.5
86	2.0
87	2.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	285321.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.23670254636298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.95099998424318	26.762627945435963
2	8.091999998321231	5.702707938920299
3	3.0449999993682835	3.2188727769424696
4	1.7790000202241072	2.5074437817044233
5	1.2629999998894992	2.225197765608138
6	1.0699999997793475	2.262196303009999
7	0.8129999998313453	2.0053207414975183
8	0.7829999998375587	2.207227047046265
9	0.6869999998574748	2.178685317989632
>10	6.374999998677441	39.35375886371569
>50	0.0879999999817435	2.0831938541087993
>100	0.044999999990664286	3.2287390536534044
>500	0.004999999998962699	1.4260293517554652
>1k	0.003999999999170159	4.837999258611944
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGC	4769	1.671450751960073	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCG	4033	1.413495676799114	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTC	2936	1.029016441131217	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGGTATCTGT	1992	0.698161018642161	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	933	0.3270001156592049	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTA	892	0.3126303356570319	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTC	838	0.29370428394685283	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCT	759	0.26601617125973903	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT	625	0.21905152442336875	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	487	0.17068494783068894	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCCGGGAACTGT	446	0.15631516782851596	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTT	385	0.13493573904479517	No Hit
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT	384	0.13458525660571777	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGC	357	0.12512223075062823	No Hit
TCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	325	0.11390679270015175	TruSeq Adapter, Index 13 (95% over 21bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	324	0.11355631026107438	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCTGTCGAATGC	317	0.11110293318753264	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCACCGGTCGAATGC	287	0.10058846001521093	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14509972977803948	0.0
2	0.0	0.0	0.0	0.8344986874432656	0.0
3	0.0	0.0	0.0	1.0160485908853536	0.0
4	0.0	0.0	0.0	1.5154860665706344	0.0
5	0.0	0.0	0.0	3.087750288271806	0.0
6	0.0	0.0	0.0	3.4708275941833935	0.0
7	0.0	0.0	0.0	4.004261866459181	0.0
8	0.0	0.0	0.0	4.663519334363752	0.0
9	0.0	0.0	0.0	4.826844150973815	0.0
10	0.0	0.0	0.0	6.784989538099193	0.0
11	0.0	0.0	0.0	7.531166650894957	0.0
12	0.0	0.0	0.0	10.197637047395741	0.0
13	0.0	0.0	0.0	10.508164488418307	0.0
14	0.0	0.0	0.0	10.679900883566228	0.0
15	0.0	0.0	0.0	11.171978228030884	0.0
16	0.0	0.0	0.0	11.613586101268396	0.0
17	0.0	0.0	0.0	12.14351554915341	0.0
18	0.0	0.0	0.0	12.690969118992292	0.0
19	0.0	0.0	0.0	13.948500110401968	0.0
20	0.0	0.0	0.0	14.424104780229987	0.0
21	0.0	0.0	0.0	15.021677338856936	0.0
22	0.0	0.0	0.0	15.690047350177519	0.0
23	0.0	0.0	0.0	16.36122122101072	0.0
24	0.0	0.0	0.0	16.892202116212967	0.0
25	0.0	0.0	0.0	17.382877530921313	0.0
26	0.0	0.0	0.0	17.80065259830156	0.0
27	0.0	0.0	0.0	18.25242446227232	0.0
28	0.0	0.0	0.0	18.710505010146466	0.0
29	0.0	0.0	0.0	19.165080733629843	0.0
30	0.0	0.0	0.0	19.685897638098844	0.0
31	0.0	0.0	0.0	20.18743800841859	0.0
32	0.0	0.0	0.0	20.67320666897985	0.0
33	0.0	0.0	0.0	21.163882083688197	0.0
34	0.0	0.0	0.0	21.635981929125442	0.0
35	0.0	0.0	0.0	22.155046421399057	0.0
36	0.0	0.0	0.0	22.627847231714455	0.0
37	0.0	0.0	0.0	23.148313653744378	0.0
38	0.0	0.0	0.0	23.676490689434004	0.0
39	0.0	0.0	0.0	24.162259349995267	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATGT	20	7.024362E-4	45.000004	45
CCGATCG	40	6.7848305E-9	45.000004	40
GCGCGAT	20	7.024362E-4	45.000004	42
CGCGCGA	20	7.024362E-4	45.000004	41
CGCGATA	20	7.024362E-4	45.000004	43
AGGCGAC	40	6.7848305E-9	45.000004	31
TACGAAT	40	6.7848305E-9	45.000004	12
CGAATAC	20	7.024362E-4	45.000004	45
GCGATAG	20	7.024362E-4	45.000004	9
TACTCCC	20	7.024362E-4	45.000004	25
GAACCGA	20	7.024362E-4	45.000004	9
AACTAGC	20	7.024362E-4	45.000004	44
GACGAGG	20	7.024362E-4	45.000004	44
ACGAGGA	20	7.024362E-4	45.000004	45
CGATCGA	40	6.7848305E-9	45.000004	41
ACAGTTC	25	3.8834107E-5	45.0	29
GGTCGAA	105	0.0	45.0	42
TACGGAG	55	1.8189894E-12	45.0	29
GTGGCGT	50	2.1827873E-11	45.0	34
CGTTCAG	50	2.1827873E-11	45.0	38
>>END_MODULE