##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934089.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	763197
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.533353773665254	31.0	28.0	31.0	25.0	33.0
2	28.82869298490429	31.0	28.0	31.0	16.0	34.0
3	28.926798716451977	31.0	28.0	33.0	16.0	34.0
4	33.504454288997465	35.0	32.0	37.0	28.0	37.0
5	34.27249583004126	35.0	35.0	37.0	32.0	37.0
6	33.11748735909601	35.0	32.0	37.0	28.0	37.0
7	34.18600308963479	35.0	33.0	36.0	32.0	37.0
8	33.318516713247035	35.0	33.0	37.0	28.0	37.0
9	35.41723041364156	37.0	34.0	39.0	30.0	39.0
10	35.111528216174854	37.0	34.0	39.0	30.0	39.0
11	35.293263731382595	37.0	35.0	39.0	30.0	39.0
12	35.361052257805	37.0	35.0	39.0	30.0	39.0
13	35.206034614915936	37.0	34.0	39.0	29.0	39.0
14	36.005432411290926	38.0	34.0	40.0	29.0	41.0
15	36.130190501272935	38.0	34.0	40.0	30.0	41.0
16	36.49190969042069	38.0	35.0	40.0	31.0	41.0
17	35.92620122982664	38.0	34.0	40.0	29.0	41.0
18	36.20503225248527	38.0	35.0	40.0	30.0	41.0
19	36.07442770346319	38.0	34.0	40.0	30.0	41.0
20	35.66081758707123	38.0	34.0	40.0	27.0	41.0
21	35.777681253988156	38.0	34.0	40.0	29.0	41.0
22	36.00151468100635	38.0	34.0	40.0	30.0	41.0
23	35.60467218817684	38.0	34.0	40.0	27.0	41.0
24	35.65685268678991	38.0	34.0	40.0	27.0	41.0
25	35.55201867931871	38.0	34.0	40.0	28.0	41.0
26	35.556212878195275	38.0	34.0	40.0	27.0	41.0
27	35.198758642919195	38.0	33.0	40.0	27.0	41.0
28	35.07587818086287	38.0	33.0	40.0	26.0	41.0
29	35.265708591621824	38.0	33.0	40.0	27.0	41.0
30	34.97779210348049	37.0	33.0	40.0	25.0	41.0
31	35.1364038380654	38.0	33.0	40.0	26.0	41.0
32	34.653797119223476	37.0	33.0	40.0	24.0	41.0
33	34.515853704875674	37.0	33.0	40.0	24.0	41.0
34	34.52918971117549	38.0	33.0	40.0	24.0	41.0
35	34.240521123641734	37.0	33.0	40.0	23.0	41.0
36	34.14870341471468	37.0	33.0	40.0	23.0	41.0
37	34.06545099102853	37.0	33.0	40.0	22.0	41.0
38	34.11054550790949	37.0	33.0	40.0	22.0	41.0
39	33.82904282904676	37.0	33.0	40.0	21.0	41.0
40	34.06079688468377	37.0	32.0	40.0	23.0	41.0
41	33.85327641487061	37.0	31.0	40.0	22.0	41.0
42	33.50726745519178	37.0	32.0	40.0	19.0	41.0
43	33.47987741041959	37.0	32.0	40.0	18.0	41.0
44	33.8378898239904	37.0	33.0	40.0	21.0	41.0
45	33.69470529889399	37.0	33.0	40.0	20.0	41.0
46	33.62004174544711	37.0	33.0	40.0	20.0	41.0
47	33.44771664458849	36.0	32.0	40.0	20.0	41.0
48	33.429018981992854	36.0	32.0	40.0	20.0	41.0
49	33.383364976539475	36.0	32.0	40.0	20.0	41.0
50	33.13411085211289	36.0	32.0	40.0	18.0	41.0
51	32.32437103395323	35.0	31.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	2.0
10	3.0
11	11.0
12	21.0
13	19.0
14	41.0
15	54.0
16	100.0
17	218.0
18	571.0
19	1370.0
20	3442.0
21	6342.0
22	9757.0
23	12908.0
24	14912.0
25	14213.0
26	13349.0
27	13628.0
28	15852.0
29	19923.0
30	25287.0
31	31641.0
32	39572.0
33	49035.0
34	62944.0
35	68916.0
36	82918.0
37	101039.0
38	117199.0
39	57909.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	37.34186586163206	4.0461374979199345	44.08114811772059	14.530848522727421
2	17.812570017963907	19.133591982148776	44.71899129582532	18.33484670406199
3	18.913989441782398	19.401412741402286	43.364688278386836	18.31990953842848
4	16.799856393565488	4.422056166363338	54.89434575869664	23.883741681374534
5	29.633764283664636	5.520724007038812	42.569873833361505	22.275637875935047
6	18.942291439824842	19.6858740272826	45.26852175781613	16.10331277507642
7	72.33820363549647	2.277131592498398	21.838791295039158	3.5458734769659737
8	73.26679743237985	15.741807161191671	6.66564465007069	4.325750756357795
9	69.21083285180629	4.310944618492997	7.460459095096024	19.017763434604696
10	44.543021002441044	24.52813624791502	15.789894352310085	15.138948397333845
11	35.80111032931209	22.408369005643365	23.415841519293185	18.37467914575136
12	22.73580739966221	20.02641519817295	27.429091047265647	29.808686354899194
13	28.500374084279684	21.551447398247113	32.35180431788909	17.596374199584115
14	19.994051339300338	32.379058093781815	27.393844577481307	20.23304598943654
15	16.798677143647055	21.921469817098338	33.695101002755514	27.584752036499093
16	19.297376693042555	23.12024287307209	26.21249821474665	31.369882219138702
17	19.77471085447139	30.428709756458687	29.135596707010116	20.66098268205981
18	19.969156063244483	21.76973966092634	28.608865076775718	29.652239199053454
19	20.268161431452167	25.60164675699721	25.791637021633996	28.338554789916625
20	29.229150533872645	23.60373533963053	30.209893382704596	16.95722074379223
21	22.726897511389588	33.802412745333115	25.53338128949668	17.93730845378061
22	21.54332367658678	20.688891596796108	35.160908651370484	22.606876075246625
23	26.400654090621423	27.09392201489262	26.655110017466	19.850313877019957
24	20.60306840828777	29.42700246463233	26.690225459481624	23.279703667598277
25	19.56742492436422	35.756691915717695	23.619720727413764	21.056162432504323
26	19.553929064186573	24.20213915935204	32.55253885956051	23.691392916900877
27	27.046620990386494	26.15772860742377	25.18157173049684	21.61407867169289
28	17.352924605311603	31.773447746780974	29.074537766788914	21.799089881118505
29	20.068606139699185	22.79870072864542	31.85599524107144	25.27669789058395
30	18.810477504497527	33.78498605209402	27.45293810117178	19.95159834223667
31	22.67579668159073	24.701485985924997	23.00284199230343	29.619875340180847
32	27.464337517049987	27.375369662092485	23.249960364099962	21.910332456757562
33	26.091559584222683	22.965630105988364	23.661911668939997	27.280898640848957
34	22.06127644631727	24.418990116575408	24.761889787302625	28.757843649804705
35	24.838672059769625	26.9568669688167	24.806308200897014	23.398152770516656
36	19.240641669188953	32.34525292945334	23.788615521287426	24.625489880070283
37	18.972951937704156	28.180666328615022	25.569413925893315	27.276967807787507
38	19.023266600890725	28.848514865755497	22.985808382370475	29.1424101509833
39	21.638842919980032	28.140047720313365	24.46393264124466	25.757176718461945
40	19.48972545751621	25.33762580303644	25.270539585454344	29.902109153993006
41	21.157184842183604	24.53154296990161	25.9294782343222	28.38179395359258
42	22.68785123631251	25.893052514619423	27.117900096567464	24.301196152500598
43	19.8812364304367	26.744864039035793	26.59509929939452	26.778800231132983
44	22.009651505443546	24.772372008799827	29.83541601971706	23.382560466039568
45	21.436536045084033	24.64173732339095	25.92266479034902	27.999061841175998
46	24.18798816033082	27.840256185493388	24.43943044849495	23.53232520568084
47	16.682979623871688	29.646998088304855	30.697840793399344	22.972181494424113
48	21.194527756267387	28.57807355112769	26.27984648786617	23.947552204738752
49	22.381508313056784	24.022500088443742	30.326770152398396	23.26922144610107
50	21.87429982036093	25.781678911211653	27.37628685647349	24.967734411953927
51	20.130844329838823	23.02446157414141	28.187479772588205	28.657214323431564
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	119.0
1	346.0
2	573.0
3	1797.0
4	3021.0
5	2207.5
6	1394.0
7	1347.0
8	1300.0
9	1335.5
10	1371.0
11	1400.0
12	1429.0
13	1467.5
14	1506.0
15	1449.5
16	1393.0
17	1470.0
18	1547.0
19	1619.0
20	1691.0
21	1869.5
22	2048.0
23	2316.0
24	2584.0
25	3564.5
26	5622.0
27	6699.0
28	7834.0
29	8969.0
30	10423.5
31	11878.0
32	14068.0
33	16258.0
34	18569.0
35	20880.0
36	22960.0
37	25040.0
38	26714.0
39	28388.0
40	30480.0
41	32572.0
42	34634.5
43	36697.0
44	39437.0
45	42177.0
46	70620.0
47	99063.0
48	86500.5
49	73938.0
50	69626.5
51	65315.0
52	60645.0
53	55975.0
54	52900.5
55	49826.0
56	46706.0
57	43586.0
58	39166.5
59	34747.0
60	30053.0
61	25359.0
62	22330.0
63	19301.0
64	17214.5
65	15128.0
66	12651.0
67	10174.0
68	8083.5
69	5993.0
70	5000.5
71	4008.0
72	3473.0
73	2938.0
74	2507.5
75	1547.5
76	1018.0
77	730.5
78	443.0
79	298.0
80	153.0
81	107.0
82	61.0
83	37.0
84	13.0
85	7.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	763197.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.917479868959642
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.82112042167131	23.132804844100278
2	8.34308865236611	5.158945509089497
3	3.008869783705807	2.790800128981358
4	1.677105550428248	2.0740750837394337
5	1.1617737276507447	1.7959557918464053
6	0.93787173461352	1.7397978284747861
7	0.8252999180058814	1.7861335520560617
8	0.7294719771592333	1.8042748135032625
9	0.6658305585117	1.8527222600031277
>10	7.6396901416474705	42.995929678530054
>50	0.11495986769994362	2.415971357541348
>100	0.06372307268921024	3.4701383720972654
>500	0.006458419529311849	1.363801120996489
>1k	0.0030139291136788626	1.966429493348964
>5k	8.611226039082465E-4	1.90279875290618
>10k+	8.611226039082465E-4	3.7494214127854675
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGC	15538	2.0359094702940395	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCG	12628	1.6546186633333202	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTC	8316	1.0896269246341377	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAACCGATT	5978	0.7832840013784123	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAACCGATTCGTA	3122	0.40906869392830425	No Hit
GCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	2551	0.3342518379920256	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTC	2194	0.2874749245607622	No Hit
GAACTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCT	2170	0.28433025811160156	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCT	1977	0.259041898749602	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1747	0.22890551194514655	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCAACCGATTCGT	1011	0.13246907417088905	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGC	925	0.12120068606139699	No Hit
CTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGCT	851	0.11150463117648524	No Hit
CCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATGCCGTCTTCTGC	822	0.10770482588374955	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCCAACCGATTCGTATG	778	0.10193960406028849	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12342815812955239	0.0
2	0.0	0.0	0.0	0.7083361176734185	0.0
3	0.0	0.0	0.0	0.886926966431996	0.0
4	0.0	0.0	0.0	1.4483809553758729	0.0
5	0.0	0.0	0.0	3.1293362002209126	0.0
6	0.0	0.0	0.0	3.4840283701324823	0.0
7	0.0	0.0	0.0	4.0533440251992605	0.0
8	0.0	0.0	0.0	4.754735671130783	0.0
9	0.0	0.0	0.0	4.935422964188801	0.0
10	0.0	0.0	0.0	7.21674744528608	0.0
11	0.0	0.0	0.0	7.865727983731592	0.0
12	0.0	0.0	0.0	10.93518449364974	0.0
13	0.0	0.0	0.0	11.285683774962427	0.0
14	0.0	0.0	0.0	11.476722261748932	0.0
15	0.0	0.0	0.0	12.106310690424621	0.0
16	0.0	0.0	0.0	12.531233744367444	0.0
17	0.0	0.0	0.0	12.969128547413053	0.0
18	0.0	0.0	0.0	13.394837767968166	0.0
19	0.0	0.0	0.0	14.719528509677057	0.0
20	0.0	0.0	0.0	15.15493378511708	0.0
21	0.0	0.0	0.0	15.634102335307922	0.0
22	0.0	0.0	0.0	16.169874881583652	0.0
23	0.0	0.0	0.0	16.694641095287324	0.0
24	0.0	0.0	0.0	17.104233900290488	0.0
25	0.0	0.0	0.0	17.48749012378193	0.0
26	0.0	0.0	0.0	17.830389794509152	0.0
27	0.0	0.0	0.0	18.192812602774907	0.0
28	0.0	0.0	0.0	18.564145299313285	0.0
29	0.0	0.0	0.0	18.96626952149969	0.0
30	0.0	0.0	0.0	19.4320732392816	0.0
31	0.0	0.0	0.0	19.82921840625684	0.0
32	0.0	0.0	0.0	20.21719162942202	0.0
33	0.0	0.0	0.0	20.616040157390557	0.0
34	0.0	0.0	0.0	21.012792241059646	0.0
35	0.0	0.0	0.0	21.459727960146594	0.0
36	0.0	0.0	0.0	21.863948626632443	0.0
37	0.0	0.0	0.0	22.281010014452363	0.0
38	0.0	0.0	0.0	22.71772556757954	0.0
39	0.0	0.0	0.0	23.213272588859756	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAATCGA	55	1.8189894E-12	45.000004	41
ACGCGAT	20	7.0317957E-4	45.0	41
CCTCGTA	105	0.0	45.0	40
CCGTCGA	20	7.0317957E-4	45.0	14
CGATCGA	625	0.0	44.999996	41
ATCGAAT	620	0.0	44.274193	43
CGATCAA	200	0.0	43.875	41
TCGAATG	510	0.0	43.67647	44
TCGAATA	165	0.0	43.636364	44
TACGGCT	1880	0.0	43.085106	7
CGTTTGG	410	0.0	42.80488	2
GATGAAT	5225	0.0	42.717705	20
CCGATCG	660	0.0	42.613636	40
CGGCGAA	560	0.0	42.589283	31
CCGATGA	4740	0.0	42.246838	18
ACACGAC	80	0.0	42.1875	26
TCGTAAG	80	0.0	42.1875	42
TGAATGA	5220	0.0	42.068966	22
CGATGAA	4790	0.0	42.04071	19
CCGCGTA	75	0.0	42.0	40
>>END_MODULE