##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934088.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	683089
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.934229653822563	31.0	30.0	31.0	26.0	34.0
2	30.123224060115152	31.0	30.0	33.0	26.0	34.0
3	30.15797795016462	31.0	30.0	33.0	25.0	34.0
4	33.92434221602163	35.0	33.0	37.0	28.0	37.0
5	34.40228725685819	35.0	35.0	37.0	32.0	37.0
6	33.8535754491728	35.0	35.0	37.0	29.0	37.0
7	34.38262656842666	35.0	35.0	37.0	32.0	37.0
8	34.43891059583744	35.0	35.0	37.0	31.0	37.0
9	35.87958816493897	37.0	35.0	39.0	31.0	39.0
10	35.22851048692044	37.0	34.0	39.0	30.0	39.0
11	35.3765644008321	37.0	35.0	39.0	30.0	39.0
12	35.431159043697086	37.0	35.0	39.0	30.0	39.0
13	35.453629029306576	37.0	35.0	39.0	30.0	39.0
14	36.123871120747076	38.0	35.0	40.0	29.0	41.0
15	36.36398917271395	38.0	35.0	40.0	30.0	41.0
16	36.51722396349524	38.0	35.0	40.0	31.0	41.0
17	36.24176351837023	38.0	35.0	40.0	30.0	41.0
18	36.3630786032274	38.0	35.0	40.0	30.0	41.0
19	36.32709793306582	38.0	35.0	40.0	30.0	41.0
20	36.25750817243434	38.0	34.0	40.0	30.0	41.0
21	35.991725821964636	38.0	34.0	40.0	29.0	41.0
22	36.20077471603261	38.0	34.0	40.0	30.0	41.0
23	36.19760821796282	38.0	34.0	40.0	30.0	41.0
24	36.13013970361109	38.0	34.0	40.0	30.0	41.0
25	35.70815369593128	38.0	34.0	40.0	29.0	41.0
26	35.79918282976303	38.0	34.0	40.0	29.0	41.0
27	35.824059529578136	38.0	34.0	40.0	29.0	41.0
28	35.73162647912644	38.0	34.0	40.0	29.0	41.0
29	35.858896864098234	38.0	34.0	40.0	29.0	41.0
30	35.38915866014531	38.0	34.0	40.0	27.0	41.0
31	35.54359534409133	38.0	34.0	40.0	28.0	41.0
32	35.497110918196604	38.0	34.0	40.0	28.0	41.0
33	35.49316121325332	38.0	34.0	40.0	27.0	41.0
34	35.43324954727715	38.0	34.0	40.0	27.0	41.0
35	35.36701806060411	38.0	34.0	40.0	27.0	41.0
36	35.15284684718975	38.0	34.0	40.0	26.0	41.0
37	35.18990204790298	38.0	34.0	40.0	27.0	41.0
38	35.177363418236865	38.0	34.0	40.0	27.0	41.0
39	35.045963263937786	38.0	34.0	40.0	26.0	41.0
40	34.92759069462398	37.0	33.0	40.0	26.0	41.0
41	34.78847558663659	37.0	33.0	40.0	25.0	41.0
42	34.97787550377769	37.0	33.0	40.0	26.0	41.0
43	35.01799472689503	37.0	34.0	40.0	27.0	41.0
44	35.02836087244854	37.0	34.0	40.0	27.0	41.0
45	35.070394926576185	37.0	34.0	40.0	27.0	41.0
46	34.95806988547612	37.0	34.0	40.0	27.0	41.0
47	34.86246155332614	37.0	33.0	40.0	26.0	41.0
48	34.83153878923537	37.0	33.0	40.0	26.0	41.0
49	34.8423719310368	37.0	33.0	40.0	27.0	41.0
50	34.652298602378316	37.0	33.0	40.0	26.0	41.0
51	33.578113540109705	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	9.0
10	9.0
11	11.0
12	17.0
13	10.0
14	28.0
15	33.0
16	92.0
17	215.0
18	384.0
19	750.0
20	1356.0
21	2303.0
22	3529.0
23	4683.0
24	6110.0
25	7520.0
26	9148.0
27	10615.0
28	13286.0
29	16847.0
30	21622.0
31	27581.0
32	34891.0
33	45248.0
34	59325.0
35	65126.0
36	79441.0
37	100678.0
38	117346.0
39	54876.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	27.41194778425652	4.688700886707296	52.55479154253692	15.344559786499271
2	20.715602212888804	6.821951458741101	51.41687247196193	21.04557385640817
3	21.860987367678298	6.934821084807396	50.2037069840094	21.0004845635049
4	18.753925183980417	4.850466044688174	49.74959339119793	26.64601538013348
5	20.37918924181183	5.683885994357983	50.08527439323427	23.851650370595927
6	20.996824718301717	8.766207624482316	52.26756689099078	17.969400766225192
7	82.42571612191091	2.2458274104838463	11.38855990947007	3.9398965581351773
8	84.33556974274215	4.99305361380435	6.610705193613131	4.060671449840358
9	79.62242109007758	4.45505636893582	8.282376088620955	7.640146452365651
10	41.420517677784304	24.961754617626696	17.6116142991616	16.006113405427406
11	30.42575711217718	26.13832165354734	23.942853713059353	19.493067521216123
12	24.61114144716135	22.153921377741405	30.085245114472634	23.149692060624606
13	23.889419973092817	23.59048381689648	31.944739265308037	20.575356944702666
14	20.770207103320356	26.327755241264317	31.887499286330183	21.01453836908514
15	18.970734413817233	24.603528969138722	34.248099442385985	22.17763717465806
16	22.486674503615195	23.988235793578873	29.77269433412044	23.752395368685487
17	22.87783875893185	25.515855181389245	28.122836116523615	23.483469943155285
18	24.151025708216643	23.692373907353215	29.128415184551354	23.028185199878788
19	24.535748635975693	25.828259567933316	26.395535574427342	23.24045622166365
20	26.435501084046148	26.5369519930785	28.584708581165852	18.4428383417095
21	25.313392544748925	28.030754411211422	27.45132771864281	19.204525325396837
22	24.28482964884517	23.28642387741568	29.90093531004013	22.52781116369902
23	23.496645385886758	26.705890447657627	28.23336344165987	21.564100724795743
24	23.00154152679958	26.372112565126944	28.350478488161862	22.275867419911606
25	22.301339942525793	28.253858574797718	25.995002115390527	23.449799367285962
26	21.097104476869045	26.602975600544	27.990642507784493	24.309277414802462
27	22.292409920230014	26.984038683099858	27.697854891529506	23.02569650514062
28	18.891828151236513	27.331284795978267	29.51328450611853	24.263602546666686
29	20.883369517002908	25.140647851158487	29.313456958024503	24.662525673814102
30	21.602602296333274	27.403456943385123	28.51766021704346	22.476280543238143
31	22.519759504252008	26.428181393639775	26.596241485370136	24.45581761673808
32	23.288326996921338	27.84132082349445	26.16716123374846	22.703190945835754
33	23.419642242811697	26.164818932818417	25.629895957920564	24.78564286644932
34	20.66041174722474	26.505916505755472	27.33494464118146	25.49872710583833
35	21.314060100513988	28.675619136013026	26.241822075893477	23.76849868757951
36	21.920423253778058	29.599656852913746	23.84125641021887	24.638663483089317
37	19.584710045103932	29.64503893343327	26.33580690071133	24.43444412075147
38	21.904173541075906	28.287382756858914	25.461982259998333	24.346461442066847
39	22.0697449380681	27.037179635450137	26.709989474285194	24.183085952196564
40	23.467805805685643	26.88756516354384	26.279298890774115	23.3653301399964
41	19.805179120143933	27.87030679750369	25.301827433906855	27.022686648445514
42	20.35679098916832	27.812334849485204	26.808805294771254	25.02206886657522
43	20.785724846981875	26.284715461674836	26.593020821591328	26.336538869751962
44	21.36193087577168	25.856513572901918	27.285756321650616	25.495799229675782
45	21.049526489227613	25.559334142403113	25.556259872432435	27.83487949593684
46	21.890851704536303	26.46287672616599	26.442674380644398	25.20359718865331
47	18.853180185890857	28.210233219975727	28.624966878400908	24.3116197157325
48	19.858466466302342	28.371998377956604	27.260722980460816	24.508812175280234
49	21.637883204092002	25.475304096537933	28.645169223922508	24.241643475447564
50	20.934900137463785	26.251484067229892	27.084172047859063	25.729443747447256
51	20.47419882328657	25.848022732030522	25.722709632273393	27.95506881240951
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	96.0
1	322.0
2	548.0
3	1717.5
4	2887.0
5	2048.0
6	1209.0
7	1192.5
8	1176.0
9	1290.5
10	1405.0
11	1504.0
12	1603.0
13	1638.5
14	1674.0
15	1667.0
16	1660.0
17	1662.0
18	1664.0
19	1710.5
20	1757.0
21	2056.0
22	2355.0
23	2496.0
24	2637.0
25	3143.5
26	4715.0
27	5780.0
28	6485.0
29	7190.0
30	8841.0
31	10492.0
32	12255.5
33	14019.0
34	16479.0
35	18939.0
36	20651.0
37	22363.0
38	24491.0
39	26619.0
40	29298.5
41	31978.0
42	34846.0
43	37714.0
44	40815.0
45	43916.0
46	54775.0
47	65634.0
48	65586.5
49	65539.0
50	63379.0
51	61219.0
52	56032.0
53	50845.0
54	46888.5
55	42932.0
56	40559.0
57	38186.0
58	35890.5
59	33595.0
60	29947.5
61	26300.0
62	22413.5
63	18527.0
64	16048.0
65	13569.0
66	10818.5
67	8068.0
68	7003.0
69	5938.0
70	4834.0
71	3730.0
72	3001.5
73	2273.0
74	1983.0
75	1301.0
76	909.0
77	699.0
78	489.0
79	353.0
80	217.0
81	146.0
82	75.0
83	45.0
84	15.0
85	9.0
86	3.0
87	1.5
88	0.0
89	1.0
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	683089.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.83196162300795
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.27168579755254	27.724038425945675
2	9.117662260945218	6.716427729733643
3	3.194143813763765	3.5293974710074574
4	1.7876519683995002	2.6337091478154004
5	1.2525623659479561	2.3067164496509576
6	0.9392899256834613	2.0757534297390734
7	0.7872918101018055	2.029825121504471
8	0.7092142903233746	2.089740281894348
9	0.6309875696506104	2.09164589549697
>10	6.17033011686386	40.845280768211914
>50	0.09117303849568902	2.307864041613627
>100	0.041552313899532504	2.5570455971328094
>500	0.0040342052329643205	1.0802325231655252
>1k	0.0024205231397785923	2.0123231170881306
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGC	3621	0.5300919792296465	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCG	3036	0.44445160147506396	No Hit
GAATCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTC	2098	0.30713420945147707	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1786	0.2614593413156997	No Hit
GCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	1504	0.22017628742374715	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCACTGAACT	1498	0.21929792457498218	No Hit
CTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGCT	997	0.14595462670310896	Illumina PCR Primer Index 5 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	986	0.14434429481370656	Illumina PCR Primer Index 5 (95% over 23bp)
TCTGTCTCTTATACACATCTGACGCCACTGAACTCGTATGCCGTCTTCTGC	960	0.1405380558023918	Illumina PCR Primer Index 5 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCACTGAACTCGTA	763	0.11169847560127596	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	708	0.10364681615426394	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.4639380812749144E-4	0.0	0.0	0.16527860937593783	0.0
2	1.4639380812749144E-4	0.0	0.0	0.7452908771770589	0.0
3	1.4639380812749144E-4	0.0	0.0	0.9979665900051091	0.0
4	1.4639380812749144E-4	0.0	0.0	1.4895569976972254	0.0
5	1.4639380812749144E-4	0.0	0.0	2.510068234153968	0.0
6	1.4639380812749144E-4	0.0	0.0	2.986872867225208	0.0
7	1.4639380812749144E-4	0.0	0.0	3.4898819919512682	0.0
8	1.4639380812749144E-4	0.0	0.0	4.218337581193666	0.0
9	1.4639380812749144E-4	0.0	0.0	4.492533183816457	0.0
10	1.4639380812749144E-4	0.0	0.0	5.55213156704324	0.0
11	1.4639380812749144E-4	0.0	0.0	6.637056079076079	0.0
12	1.4639380812749144E-4	0.0	0.0	8.18648814429745	0.0
13	1.4639380812749144E-4	0.0	0.0	8.573260585370281	0.0
14	1.4639380812749144E-4	0.0	0.0	8.728291628177294	0.0
15	1.4639380812749144E-4	0.0	0.0	9.033961899547498	0.0
16	1.4639380812749144E-4	0.0	0.0	9.48895385520774	0.0
17	1.4639380812749144E-4	0.0	0.0	10.127230858643603	0.0
18	1.4639380812749144E-4	0.0	0.0	10.805034190273888	0.0
19	1.4639380812749144E-4	0.0	0.0	11.558083939281705	0.0
20	1.4639380812749144E-4	0.0	0.0	12.09578839653398	0.0
21	1.4639380812749144E-4	0.0	0.0	12.8462030569955	0.0
22	1.4639380812749144E-4	0.0	0.0	13.67142495341017	0.0
23	1.4639380812749144E-4	0.0	0.0	14.524315279560936	0.0
24	1.4639380812749144E-4	0.0	0.0	15.17415739383887	0.0
25	1.4639380812749144E-4	0.0	0.0	15.759732626348836	0.0
26	1.4639380812749144E-4	0.0	0.0	16.268890291016252	0.0
27	1.4639380812749144E-4	0.0	0.0	16.80410605353036	0.0
28	1.4639380812749144E-4	0.0	0.0	17.354253984473473	0.0
29	2.927876162549829E-4	0.0	0.0	17.984186540846068	0.0
30	2.927876162549829E-4	0.0	0.0	18.71000704154217	0.0
31	2.927876162549829E-4	0.0	0.0	19.307147384894208	0.0
32	2.927876162549829E-4	0.0	0.0	19.89579688737485	0.0
33	2.927876162549829E-4	0.0	0.0	20.49586510688944	0.0
34	2.927876162549829E-4	0.0	0.0	21.124626512797015	0.0
35	2.927876162549829E-4	0.0	0.0	21.776225352772478	0.0
36	2.927876162549829E-4	0.0	0.0	22.41040332958077	0.0
37	2.927876162549829E-4	0.0	0.0	23.044727700197193	0.0
38	2.927876162549829E-4	0.0	0.0	23.695740964940146	0.0
39	2.927876162549829E-4	0.0	0.0	24.550241622980316	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGATA	30	2.1642027E-6	45.000004	14
CCGATCG	30	2.1642027E-6	45.000004	40
AATTCCG	30	2.1642027E-6	45.000004	11
CGATCGA	30	2.1642027E-6	45.000004	41
ACGAACG	30	2.1642027E-6	45.000004	21
ACCGGTA	40	6.8084773E-9	45.0	41
TATTGCG	20	7.031274E-4	45.0	1
CCGATGA	680	0.0	43.676468	18
CGAACAC	95	0.0	42.631577	34
CGATGAA	705	0.0	42.127663	19
CGTTTTT	1430	0.0	40.751747	1
AACACGT	135	0.0	40.000004	41
TTTACGG	85	0.0	39.705883	2
CCGGGAT	210	0.0	39.64286	5
TACGGCT	460	0.0	39.619564	7
TTTCGCG	245	0.0	39.489796	1
TTTAGCG	40	3.4554796E-7	39.375	1
TTTGTCG	200	0.0	39.375	1
CGCTCGA	80	0.0	39.375	41
GGGCGTA	40	3.4554796E-7	39.375	7
>>END_MODULE