##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934087.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	452452
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.736409608091023	31.0	28.0	31.0	25.0	33.0
2	29.458939290797698	31.0	30.0	33.0	25.0	34.0
3	29.555190384836404	31.0	30.0	33.0	25.0	34.0
4	33.76455181986155	35.0	33.0	37.0	28.0	37.0
5	34.3748265009327	35.0	35.0	37.0	32.0	37.0
6	33.46648705277024	35.0	33.0	37.0	28.0	37.0
7	34.43790280515944	35.0	35.0	37.0	32.0	37.0
8	34.050803179121765	35.0	35.0	37.0	29.0	37.0
9	35.82792428810128	37.0	35.0	39.0	31.0	39.0
10	35.06096779326868	37.0	34.0	39.0	30.0	39.0
11	35.270050745714464	37.0	34.0	39.0	30.0	39.0
12	35.39888430153917	37.0	35.0	39.0	30.0	39.0
13	35.35613059506865	37.0	34.0	39.0	30.0	39.0
14	36.09314137190243	38.0	34.0	40.0	30.0	41.0
15	36.213841468266246	38.0	34.0	40.0	30.0	41.0
16	36.53338254665688	38.0	35.0	40.0	31.0	41.0
17	36.14035743018044	38.0	34.0	40.0	30.0	41.0
18	36.333482888792624	38.0	35.0	40.0	30.0	41.0
19	36.320920230212266	38.0	35.0	40.0	30.0	41.0
20	36.140196087098744	38.0	34.0	40.0	29.0	41.0
21	36.04993015833724	38.0	34.0	40.0	30.0	41.0
22	36.314188024365016	38.0	35.0	40.0	30.0	41.0
23	36.19415761229921	38.0	34.0	40.0	30.0	41.0
24	36.07079646017699	38.0	34.0	40.0	30.0	41.0
25	35.75070946752363	38.0	34.0	40.0	29.0	41.0
26	35.87783234464651	38.0	34.0	40.0	29.0	41.0
27	35.74779645133628	38.0	34.0	40.0	29.0	41.0
28	35.68682202752999	38.0	34.0	40.0	29.0	41.0
29	35.745418298515645	38.0	34.0	40.0	29.0	41.0
30	35.34445421834803	38.0	34.0	40.0	27.0	41.0
31	35.59486973203787	38.0	34.0	40.0	29.0	41.0
32	35.43419854481802	38.0	34.0	40.0	27.0	41.0
33	35.46903317921017	38.0	34.0	40.0	27.0	41.0
34	35.48259925914793	38.0	34.0	40.0	27.0	41.0
35	35.41992962789423	38.0	34.0	40.0	27.0	41.0
36	35.29792110544323	38.0	34.0	40.0	27.0	41.0
37	35.122245011625544	38.0	34.0	40.0	27.0	41.0
38	35.12950324012271	38.0	34.0	40.0	27.0	41.0
39	34.99691016947654	38.0	33.0	40.0	26.0	41.0
40	35.02466559988684	38.0	33.0	40.0	26.0	41.0
41	35.142649386012216	38.0	33.0	40.0	26.0	41.0
42	35.253905386648746	38.0	34.0	40.0	27.0	41.0
43	35.25093932616056	38.0	34.0	40.0	27.0	41.0
44	35.27447331429632	38.0	34.0	40.0	27.0	41.0
45	35.35428730561474	38.0	34.0	40.0	27.0	41.0
46	35.17434998629689	38.0	34.0	40.0	27.0	41.0
47	34.99122337839152	37.0	33.0	40.0	26.0	41.0
48	35.00275388328485	37.0	33.0	40.0	26.0	41.0
49	35.10276228196582	37.0	34.0	40.0	27.0	41.0
50	34.886575813566964	37.0	33.0	40.0	26.0	41.0
51	33.85432929901956	36.0	32.0	40.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	3.0
12	5.0
13	4.0
14	4.0
15	8.0
16	22.0
17	63.0
18	166.0
19	397.0
20	956.0
21	1691.0
22	2459.0
23	3023.0
24	3641.0
25	4417.0
26	5119.0
27	6362.0
28	8546.0
29	11455.0
30	15254.0
31	19444.0
32	24410.0
33	30614.0
34	39547.0
35	44343.0
36	54494.0
37	67108.0
38	75828.0
39	33068.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	31.190269907084065	4.179669887634489	52.905943613908214	11.724116591373228
2	18.41874939220072	13.370700096363814	49.268430684359885	18.94211982707558
3	18.817465720120587	13.093322606596944	49.33340995287898	18.75580172040349
4	17.552580163199632	3.878643480413392	54.64999602167744	23.91878033470954
5	24.536083385640907	4.895104895104895	48.709697382263755	21.859114336990444
6	18.445271542616677	13.9384951331854	51.97965751063096	15.636575813566964
7	77.76626028838419	1.5639228028608558	16.866319521186778	3.803497387568184
8	79.42234756394049	9.341764430260005	6.31426096027866	4.921627045520851
9	75.03293167009981	3.5391599550891586	7.981620149761742	13.446288225049289
10	40.01993581639599	22.516863667306144	20.600638299753342	16.862562216544518
11	29.62258979958095	26.535853527003972	24.480828905607666	19.360727767807415
12	20.91426272842202	21.999460716274875	30.835094109430393	26.25118244587271
13	22.311095983662355	25.737536799483703	34.855410076649015	17.095957140204927
14	20.56836968341393	30.20519303705144	29.280675077135253	19.94576220239937
15	16.9529585458789	26.310857284308614	36.01354397814575	20.72264019166674
16	17.900462369488917	25.98551890587289	28.80835978181111	27.305658942827083
17	18.4313474136483	28.496945532343766	30.719280719280718	22.35242633472722
18	19.452671222582726	25.322465145473995	31.099210523989285	24.125653107953994
19	20.219603405444115	29.281559148815784	27.839417219948192	22.65942022579191
20	22.454536613828648	26.950483145173408	32.462449055369405	18.13253118562853
21	22.060461662231575	32.09202302122656	27.965618452344117	17.88189686419775
22	19.832822045211426	23.912812850865947	32.119208225402915	24.13515687851971
23	19.962559564329478	30.188837710961604	27.81134794409131	22.037254780617612
24	19.88918161484533	27.85113116971524	28.067507713525412	24.192179501914016
25	17.690053309522334	33.62478229734867	26.18819233863482	22.496972054494176
26	18.804867698673007	27.053035460115105	30.967041807749773	23.175055033462115
27	21.501507342215305	29.81730658721809	26.887935073775783	21.79325099679082
28	18.487706983282205	28.784931882277014	31.242209118315312	21.48515201612547
29	20.512673167540424	25.19250660843581	30.816528604139226	23.47829161988454
30	18.899021332649653	31.821497086983815	29.417706187617693	19.861775392748843
31	22.01714214988551	26.864286156321555	25.92164472695446	25.196926966838472
32	21.259492719669712	30.999089406169052	27.287093437535916	20.45432443662532
33	19.41863446288225	27.023198040897157	26.569669268784313	26.98849822743628
34	21.775569563180184	26.729907260880715	26.162112224059126	25.33241095187998
35	19.07915093755802	29.050595422276835	27.03093366810181	24.839319972063333
36	23.3620361938946	27.992361620680207	25.49662726653877	23.148974918886424
37	17.79923616206802	29.285316453458048	30.742266582974548	22.173180801499385
38	20.647494098821532	27.47296950836774	28.11944692475666	23.76008946805407
39	18.55754864604422	26.332738058401777	31.352276042541526	23.757437253012476
40	18.423611786443644	26.820966643975492	29.875876336053324	24.879545233527534
41	18.20701422471334	27.16089220513999	24.380928805707565	30.251164764439096
42	20.525271188988004	27.278252720730595	28.054246638317437	24.142229451963964
43	21.38702005958643	25.721844527154264	26.679736193010527	26.211399220248776
44	20.36193894600974	25.44446703738739	29.729120437085037	24.46447357951783
45	20.75314066464509	24.902531097221363	27.283999186654057	27.060329051479492
46	23.76672000565806	26.65011979171271	27.54612643993175	22.03703376269748
47	17.440966113532486	26.546683405090484	33.297233739711615	22.715116741665415
48	19.71325135041949	27.18719333763582	27.736643887086366	25.362911424858325
49	20.28259351268201	23.358057871332207	32.66534350605147	23.694005109934313
50	21.12069346582621	23.621953267970966	28.583584557035884	26.67376870916694
51	19.161811639687745	23.227215262613495	27.47915801013146	30.131815087567297
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	60.0
1	127.0
2	194.0
3	376.5
4	559.0
5	450.0
6	341.0
7	367.5
8	394.0
9	504.5
10	615.0
11	681.5
12	748.0
13	770.0
14	792.0
15	877.0
16	962.0
17	1008.5
18	1055.0
19	1049.0
20	1043.0
21	1275.0
22	1507.0
23	1738.0
24	1969.0
25	2645.5
26	3971.0
27	4620.0
28	5417.5
29	6215.0
30	6986.0
31	7757.0
32	8717.5
33	9678.0
34	11523.5
35	13369.0
36	15583.5
37	17798.0
38	19270.5
39	20743.0
40	22935.5
41	25128.0
42	27414.5
43	29701.0
44	32588.0
45	35475.0
46	52939.5
47	70404.0
48	64254.0
49	58104.0
50	52346.0
51	46588.0
52	38557.0
53	30526.0
54	25788.0
55	21050.0
56	18172.5
57	15295.0
58	12970.0
59	10645.0
60	8433.5
61	6222.0
62	5048.0
63	3874.0
64	3250.5
65	2627.0
66	2016.5
67	1406.0
68	1055.5
69	705.0
70	554.0
71	403.0
72	307.0
73	211.0
74	202.5
75	144.0
76	94.0
77	67.5
78	41.0
79	27.0
80	13.0
81	7.5
82	2.0
83	1.5
84	1.0
85	1.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	452452.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.28370029332481
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.01041727349764	28.333926334824927
2	11.240665251489169	8.606685191712039
3	4.022192137098834	4.619531948965782
4	1.9959504288819319	3.0564947207859596
5	1.276569530758843	2.4435902659580915
6	0.920109735377414	2.1135123207695594
7	0.744014103069059	1.9938529055131913
8	0.6057555535047071	1.855245124911303
9	0.5382365976393587	1.8545119731831656
>10	4.473340215207551	30.01755578453694
>50	0.11088629378496317	2.9159627006445894
>100	0.053108698602271825	3.775267929874463
>500	0.0017508362176573127	0.3970320832921182
>1k	0.005836120725524376	4.520624727771202
>5k	0.0011672241451048753	3.4962059872566487
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC	8541	1.8877140558556487	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCG	7107	1.5707743583849778	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTC	4850	1.0719369126448772	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCGAGTTCACT	3337	0.7375367994837022	No Hit
GCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	2427	0.5364104921627045	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTA	1692	0.3739623208649757	No Hit
TCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	1417	0.31318239282841054	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTC	1399	0.30920407026601715	No Hit
CCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGC	1364	0.30146844306136344	TruSeq Adapter, Index 19 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCT	1359	0.3003633534606986	No Hit
CTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCTGCT	1320	0.291743654575513	TruSeq Adapter, Index 13 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGCCGTCTTCT	1068	0.23604713870200594	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGAGTTCACTCGT	644	0.14233554056562905	No Hit
TCGCAGGGGAGGCTAGTATTTGGAGAAAGAAGATTAGAAAATGGAAGTGAA	606	0.1339368596005764	No Hit
GAATGATCCGGCTGTCTCTTATACACATCTGACGCGAGTTCACTCGTATGC	527	0.11647644391007224	No Hit
TGTTTTGGGATAAAAAAGATATTTATGAATGTACAGTTTGCTAAAGCCAAG	457	0.10100518950076473	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.2101792013296437E-4	0.0	0.0	0.3220231096337291	0.0
2	2.2101792013296437E-4	0.0	0.0	1.649456737952313	0.0
3	2.2101792013296437E-4	0.0	0.0	2.0948078470202365	0.0
4	2.2101792013296437E-4	0.0	0.0	3.070601964407274	0.0
5	2.2101792013296437E-4	0.0	0.0	5.330731215686968	0.0
6	2.2101792013296437E-4	0.0	0.0	5.955548875902858	0.0
7	2.2101792013296437E-4	0.0	0.0	6.814203495619425	0.0
8	2.2101792013296437E-4	0.0	0.0	8.049472651242564	0.0
9	2.2101792013296437E-4	0.0	0.0	8.39381857080972	0.0
10	2.2101792013296437E-4	0.0	0.0	11.020837569510135	0.0
11	2.2101792013296437E-4	0.0	0.0	12.674051612104709	0.0
12	2.2101792013296437E-4	0.0	0.0	16.28725257043841	0.0
13	2.2101792013296437E-4	0.0	0.0	16.882011793516217	0.0
14	2.2101792013296437E-4	0.0	0.0	17.132425097026868	0.0
15	2.2101792013296437E-4	0.0	0.0	17.814928434397462	0.0
16	2.2101792013296437E-4	0.0	0.0	18.645955814097405	0.0
17	2.2101792013296437E-4	0.0	0.0	19.731374819870396	0.0
18	2.2101792013296437E-4	0.0	0.0	20.887077524245665	0.0
19	2.2101792013296437E-4	0.0	0.0	22.59112568847082	0.0
20	2.2101792013296437E-4	0.0	0.0	23.512328379585018	0.0
21	2.2101792013296437E-4	0.0	0.0	24.700741736139967	0.0
22	2.2101792013296437E-4	0.0	0.0	25.938884124724833	0.0
23	2.2101792013296437E-4	0.0	0.0	27.271401165206473	0.0
24	2.2101792013296437E-4	0.0	0.0	28.22155720385809	0.0
25	2.2101792013296437E-4	0.0	0.0	29.10142954390742	0.0
26	2.2101792013296437E-4	0.0	0.0	29.853332508199763	0.0
27	2.2101792013296437E-4	0.0	0.0	30.607887687533704	0.0
28	2.2101792013296437E-4	0.0	0.0	31.472730809013996	0.0
29	2.2101792013296437E-4	0.0	0.0	32.30972567255753	0.0
30	2.2101792013296437E-4	0.0	0.0	33.21921441390468	0.0
31	2.2101792013296437E-4	0.0	0.0	34.06814424513539	0.0
32	2.2101792013296437E-4	0.0	0.0	34.90668623411986	0.0
33	2.2101792013296437E-4	0.0	0.0	35.725999664052765	0.0
34	2.2101792013296437E-4	0.0	0.0	36.51613872852811	0.0
35	2.2101792013296437E-4	0.0	0.0	37.31533952772891	0.0
36	2.2101792013296437E-4	0.0	0.0	38.13244277846048	0.0
37	2.2101792013296437E-4	0.0	0.0	38.97032171368455	0.0
38	2.2101792013296437E-4	0.0	0.0	39.79493957370064	0.0
39	2.2101792013296437E-4	0.0	0.0	40.66287694606279	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCG	30	2.1625729E-6	45.000004	1
CGAACAC	30	2.1625729E-6	45.000004	34
CCCCGCG	45	3.8380676E-10	45.000004	38
ACGGCGG	30	2.1625729E-6	45.000004	30
TAGGGCG	30	2.1625729E-6	45.000004	5
GTATACG	30	2.1625729E-6	45.000004	23
TATACGA	30	2.1625729E-6	45.000004	24
GCCCCGA	20	7.0287473E-4	45.0	24
TTCTACG	20	7.0287473E-4	45.0	1
AGCCGTA	25	3.8870436E-5	45.0	13
CGGCGAA	40	6.7993824E-9	45.0	31
TCGTTGT	25	3.8870436E-5	45.0	25
CTGTCGT	20	7.0287473E-4	45.0	42
CCGGCGA	25	3.8870436E-5	45.0	8
GCACGGG	20	7.0287473E-4	45.0	3
CCTCGTG	20	7.0287473E-4	45.0	1
CGCTGGA	20	7.0287473E-4	45.0	41
AGGCGAC	25	3.8870436E-5	45.0	31
TAGTTCG	20	7.0287473E-4	45.0	40
CTGCGCA	20	7.0287473E-4	45.0	1
>>END_MODULE