##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934086.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	227725
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.016923921396423	31.0	30.0	31.0	26.0	34.0
2	30.160676254254035	31.0	30.0	33.0	26.0	34.0
3	30.240992425074104	31.0	30.0	34.0	25.0	34.0
4	33.97188275332089	35.0	33.0	37.0	28.0	37.0
5	34.43258315951257	35.0	35.0	37.0	32.0	37.0
6	33.862645734987375	35.0	35.0	37.0	30.0	37.0
7	34.4277308156768	35.0	35.0	37.0	32.0	37.0
8	34.47657481611593	35.0	35.0	37.0	32.0	37.0
9	35.95951696124712	37.0	35.0	39.0	31.0	39.0
10	35.225631792732464	37.0	34.0	39.0	30.0	39.0
11	35.38806894280382	37.0	35.0	39.0	30.0	39.0
12	35.337841695026896	37.0	35.0	39.0	30.0	39.0
13	35.23104182676474	37.0	34.0	39.0	30.0	39.0
14	35.94280382039741	38.0	34.0	40.0	28.0	41.0
15	36.27340871665386	38.0	35.0	40.0	30.0	41.0
16	36.491219672851024	38.0	35.0	40.0	31.0	41.0
17	36.24681523767702	38.0	34.0	40.0	30.0	41.0
18	36.39646503458118	38.0	35.0	40.0	30.0	41.0
19	36.38437589197497	38.0	35.0	40.0	30.0	41.0
20	36.31970139422549	38.0	34.0	40.0	30.0	41.0
21	36.07145021407399	38.0	34.0	40.0	30.0	41.0
22	36.27767702272478	38.0	34.0	40.0	30.0	41.0
23	36.28619606982106	38.0	34.0	40.0	30.0	41.0
24	36.20422000219563	38.0	34.0	40.0	30.0	41.0
25	35.78838511362389	38.0	34.0	40.0	29.0	41.0
26	35.91055000548908	38.0	34.0	40.0	30.0	41.0
27	35.90910088923043	38.0	34.0	40.0	30.0	41.0
28	35.817108354374795	38.0	34.0	40.0	29.0	41.0
29	35.96321440333736	38.0	34.0	40.0	30.0	41.0
30	35.522560105390276	38.0	34.0	40.0	29.0	41.0
31	35.722151718081015	38.0	34.0	40.0	29.0	41.0
32	35.696014930288726	38.0	34.0	40.0	29.0	41.0
33	35.698223734767815	38.0	34.0	40.0	29.0	41.0
34	35.71639038313756	38.0	34.0	40.0	29.0	41.0
35	35.679121747722036	38.0	34.0	40.0	29.0	41.0
36	35.43145460533538	38.0	34.0	40.0	28.0	41.0
37	35.50894719508179	38.0	34.0	40.0	29.0	41.0
38	35.43417718739708	38.0	34.0	40.0	28.0	41.0
39	35.370262377868045	38.0	34.0	40.0	28.0	41.0
40	35.26901745526403	38.0	34.0	40.0	27.0	41.0
41	35.16979690416072	37.0	34.0	40.0	27.0	41.0
42	35.346079701394224	38.0	34.0	40.0	28.0	41.0
43	35.378434515314524	38.0	34.0	40.0	28.0	41.0
44	35.375769019650896	38.0	34.0	40.0	28.0	41.0
45	35.46182896036887	38.0	34.0	40.0	29.0	41.0
46	35.32716653858821	37.0	34.0	40.0	28.0	41.0
47	35.27823471292129	37.0	34.0	40.0	28.0	41.0
48	35.2246657152267	37.0	34.0	40.0	28.0	41.0
49	35.22628169941816	37.0	34.0	40.0	28.0	41.0
50	35.08131298715556	37.0	34.0	40.0	27.0	41.0
51	33.93781534745856	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	8.0
10	6.0
11	2.0
12	5.0
13	6.0
14	4.0
15	12.0
16	21.0
17	41.0
18	89.0
19	172.0
20	360.0
21	595.0
22	989.0
23	1275.0
24	1586.0
25	2094.0
26	2651.0
27	3293.0
28	4073.0
29	5495.0
30	7077.0
31	9111.0
32	11546.0
33	15396.0
34	21564.0
35	21990.0
36	25660.0
37	32862.0
38	40048.0
39	19694.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.294982983862113	4.281479855088374	54.496432100120764	15.927105060928751
2	18.821385442968495	6.914919310571961	53.52245032385553	20.741244922604018
3	20.596333296739488	6.647491491931056	52.39082226369525	20.365352947634207
4	18.416511142825776	4.794818311559996	51.184981886046764	25.60368865956746
5	19.95257437698979	5.604127785706444	51.15951256998573	23.283785267318038
6	21.377538697990996	8.978373037655066	52.10934240860687	17.534745855747065
7	83.67372927873532	2.195191568778131	10.42836754857833	3.702711603908223
8	85.68536612141838	4.907234603139752	5.2194532879569655	4.1879459874849045
9	81.63530574157427	4.287627621034142	7.089252387748381	6.987814249643209
10	41.39686024810627	26.042814798550886	17.350312877374023	15.210012075968823
11	32.53485563728181	26.507410253595342	22.431441431551214	18.526292677571632
12	26.877154462619384	22.8503677681414	28.652541442529365	21.619936326709848
13	23.374684378087608	27.414644856735098	29.794269403886265	19.41640136129103
14	19.42167087495883	30.921945328795697	28.685475902953122	20.97090789329235
15	17.72269184323197	25.379734328685917	36.12559007574926	20.771983752332858
16	19.397518937314747	24.98408167746185	31.5894170600505	24.028982325172908
17	19.9126138983423	25.632231858601383	28.995498957075423	25.459655285980897
18	21.13162806016028	24.8953781973872	30.31946426611044	23.65352947634208
19	23.79097595784389	27.25655944670107	26.709847403666704	22.24261719178834
20	25.17466242178066	26.374355033483365	28.9853990558788	19.465583488857174
21	23.226259743111207	28.873861016577013	28.058842902623777	19.841036337688
22	22.350642221978262	25.425842573279173	28.580524755736086	23.642990449006476
23	21.453946646174114	28.039082226369526	27.368097486002856	23.138873641453507
24	21.315182786255352	26.773959819958282	29.187836205950155	22.723021187836206
25	18.917993193544845	29.00603798441102	28.216489186518828	23.859479635525304
26	18.311120869469754	30.505653749039414	28.081238335711934	23.1019870457789
27	20.79569656383796	30.457788999890216	27.412888352179166	21.333626084092653
28	18.40158085410034	29.07717641892634	30.539905587880117	21.981337139093206
29	19.39707981117576	25.876385991876166	30.118344494456036	24.60818970249204
30	20.00878252277967	30.084970907893293	28.195850257986603	21.710396311340432
31	23.171808101877264	27.954330881545726	24.944121198814358	23.929739817762652
32	22.65320013173784	29.36348666154353	26.012075968822046	21.971237237896585
33	22.40509386321221	28.923482270282136	24.768909869359973	23.90251399714568
34	20.739488418048087	27.544626193874187	26.974201339334726	24.741684048743
35	20.756175211329456	27.264024591063784	26.853880777253263	25.125919420353497
36	22.729168953781976	29.482050719069054	25.24184872104512	22.546931606103854
37	20.49094302338347	29.731913492150618	27.83708420243715	21.940059282028763
38	21.509276539686027	29.51147217038094	25.255022505214626	23.72422878471841
39	20.81897024920408	28.7390492919091	27.151169173345043	23.29081128554177
40	20.82072675376002	27.36897573828082	27.2767592490943	24.53353825886486
41	19.579317158853883	27.356241080250303	27.043144143155125	26.021297617740696
42	20.650784937973434	26.68218245691075	28.13744648150181	24.52958612361401
43	20.848830826654957	28.8132616093973	26.484575694368207	23.853331869579534
44	20.02151718081019	27.731693929081132	27.264024591063784	24.982764299044902
45	20.722801624766714	27.8199582830168	24.64244154133275	26.81479855088374
46	21.969480733340653	28.979690416072017	25.994071797123723	23.05675705346361
47	20.007904270501705	28.000000000000004	28.314853441651117	23.677242287847182
48	20.55154243056318	27.673729278735316	27.758480623559116	24.016247667142387
49	20.642002415193765	25.719617960259082	30.445932594137666	23.192447030409486
50	21.29454385772313	26.773081567680318	27.134043253924688	24.798331320671863
51	20.039082226369526	26.743220990229442	26.663739159073447	26.55395762432759
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	30.0
1	82.0
2	134.0
3	239.0
4	344.0
5	279.0
6	214.0
7	217.5
8	221.0
9	285.0
10	349.0
11	419.5
12	490.0
13	499.5
14	509.0
15	515.5
16	522.0
17	541.5
18	561.0
19	591.5
20	622.0
21	778.5
22	935.0
23	960.0
24	985.0
25	1185.0
26	1781.5
27	2178.0
28	2684.5
29	3191.0
30	3587.5
31	3984.0
32	4859.0
33	5734.0
34	6136.5
35	6539.0
36	7180.5
37	7822.0
38	9042.5
39	10263.0
40	10973.0
41	11683.0
42	13481.0
43	15279.0
44	16699.5
45	18120.0
46	20826.5
47	23533.0
48	23809.5
49	24086.0
50	23146.5
51	22207.0
52	19415.5
53	16624.0
54	14683.5
55	12743.0
56	11629.0
57	10515.0
58	9313.0
59	8111.0
60	7030.0
61	5949.0
62	5131.5
63	4314.0
64	3660.5
65	3007.0
66	2478.0
67	1949.0
68	1543.5
69	1138.0
70	859.0
71	580.0
72	542.5
73	505.0
74	355.0
75	151.5
76	98.0
77	76.5
78	55.0
79	31.5
80	8.0
81	6.0
82	4.0
83	2.0
84	0.0
85	0.0
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	227725.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.809638818750685
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.20032055004529	28.811065978702384
2	9.342407841861977	7.064661323965309
3	3.147429792571601	3.570095509935229
4	1.6178485981742585	2.446810846415633
5	1.059208845322989	2.0024151937644086
6	0.8013751132377878	1.8179822153913714
7	0.708462056630508	1.8750686134592163
8	0.6294859585143202	1.9040509386321223
9	0.5017305056793105	1.7073224283675488
>10	5.86049104550417	42.106927214842464
>50	0.09291305660727973	2.326490284334175
>100	0.02903533018977492	1.8289603688659568
>500	0.006968479245545981	1.555384784279284
>1k	0.0023228264151819934	0.9827642990449007
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGC	1206	0.5295861236140081	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCG	1032	0.45317817543089256	No Hit
GAATCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTC	740	0.32495334284773303	No Hit
CCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	661	0.2902623778680426	TruSeq Adapter, Index 15 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGCT	575	0.2524975299154682	TruSeq Adapter, Index 14 (95% over 22bp)
TCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	530	0.23273685366121416	TruSeq Adapter, Index 15 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCAGCAGTATCGTATGCCGTCTTCTGC	528	0.23185860138324735	TruSeq Adapter, Index 15 (95% over 23bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCAGCAGTAT	508	0.22307607860357886	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCAGCAGTATCGTA	264	0.11592930069162367	No Hit
GGTTGGGAGAACTGTATGATTTTAATAATTGATGAATAACACTAGTATATT	252	0.11065978702382258	No Hit
CTTTTTGGGGCATAATGAAATGCAATTAAAAGATGAAATGGAGCAGAAATG	250	0.10978153474585574	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.28543199033922495	0.0
2	0.0	0.0	0.0	1.1935448457569438	0.0
3	0.0	0.0	0.0	1.516741684048743	0.0
4	0.0	0.0	0.0	2.0840926556153256	0.0
5	0.0	0.0	0.0	3.1533648040399607	0.0
6	0.0	0.0	0.0	3.560873861016577	0.0
7	0.0	0.0	0.0	4.038643100230542	0.0
8	0.0	0.0	0.0	4.804479086617631	0.0
9	0.0	0.0	0.0	5.0604896256449665	0.0
10	0.0	0.0	0.0	6.1047315841475465	0.0
11	0.0	0.0	0.0	7.318476232297727	0.0
12	0.0	0.0	0.0	9.011746624217807	0.0
13	0.0	0.0	0.0	9.400812383357119	0.0
14	0.0	0.0	0.0	9.536063234164013	0.0
15	0.0	0.0	0.0	9.858820946316829	0.0
16	0.0	0.0	0.0	10.357229114063015	0.0
17	0.0	0.0	0.0	11.133604127785706	0.0
18	0.0	0.0	0.0	12.010099901196618	0.0
19	0.0	0.0	0.0	12.815457240092217	0.0
20	0.0	0.0	0.0	13.461850916675814	0.0
21	0.0	0.0	0.0	14.308486112635855	0.0
22	0.0	0.0	0.0	15.194642661104401	0.0
23	0.0	0.0	0.0	16.108464156328907	0.0
24	0.0	0.0	0.0	16.792183554726094	0.0
25	0.0	0.0	0.0	17.42408606872324	0.0
26	0.0	0.0	0.0	17.964650345811833	0.0
27	0.0	0.0	0.0	18.540783840158085	0.0
28	4.39126138983423E-4	0.0	0.0	19.17005159732133	0.0
29	4.39126138983423E-4	0.0	0.0	19.78834120100999	0.0
30	4.39126138983423E-4	0.0	0.0	20.427708859369854	0.0
31	4.39126138983423E-4	0.0	0.0	21.07366340981447	0.0
32	4.39126138983423E-4	0.0	0.0	21.668240201998024	0.0
33	4.39126138983423E-4	0.0	0.0	22.289164562520583	0.0
34	4.39126138983423E-4	0.0	0.0	22.87627621034142	0.0
35	4.39126138983423E-4	0.0	0.0	23.47919639916566	0.0
36	4.39126138983423E-4	0.0	0.0	24.141837742891646	0.0
37	4.39126138983423E-4	0.0	0.0	24.77593588758371	0.0
38	4.39126138983423E-4	0.0	0.0	25.41881655505544	0.0
39	4.39126138983423E-4	0.0	0.0	26.0502799429136	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGAT	115	0.0	45.000004	8
ACCGATA	30	2.1578107E-6	45.000004	11
ACCTATG	30	2.1578107E-6	45.000004	34
GGTTGTA	30	2.1578107E-6	45.000004	9
TGGGCGC	30	2.1578107E-6	45.000004	6
GGCGATA	30	2.1578107E-6	45.000004	8
TATACTA	30	2.1578107E-6	45.000004	44
CGATCGA	30	2.1578107E-6	45.000004	41
GACCTAT	30	2.1578107E-6	45.000004	33
GTCATAT	25	3.8809252E-5	45.0	11
AGGTAAC	20	7.021362E-4	45.0	25
CGGCGAA	20	7.021362E-4	45.0	31
CTCACGT	20	7.021362E-4	45.0	2
GGTACAT	25	3.8809252E-5	45.0	8
AGTCACG	20	7.021362E-4	45.0	11
CCGATCG	20	7.021362E-4	45.0	40
CCGATAG	20	7.021362E-4	45.0	12
CCAGGTA	25	3.8809252E-5	45.0	37
AGCTTAA	25	3.8809252E-5	45.0	18
CGGCAAC	20	7.021362E-4	45.0	19
>>END_MODULE