##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934085.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	319286
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.09859185808335	31.0	30.0	31.0	26.0	34.0
2	30.43914860031445	31.0	30.0	33.0	27.0	34.0
3	30.50030380286013	31.0	30.0	34.0	26.0	34.0
4	34.06373909285092	35.0	33.0	37.0	30.0	37.0
5	34.446749935794244	35.0	35.0	37.0	32.0	37.0
6	33.98539553879594	35.0	35.0	37.0	30.0	37.0
7	34.35370169691123	35.0	35.0	37.0	32.0	37.0
8	34.55132075944451	35.0	35.0	37.0	32.0	37.0
9	35.89827928565612	37.0	35.0	39.0	31.0	39.0
10	35.33405160263839	37.0	34.0	39.0	30.0	39.0
11	35.49647024924362	37.0	35.0	39.0	30.0	39.0
12	35.47950740088823	37.0	35.0	39.0	30.0	39.0
13	35.43035084532363	37.0	35.0	39.0	30.0	39.0
14	36.14466653721115	38.0	35.0	40.0	29.0	41.0
15	36.46908101200804	38.0	35.0	40.0	30.0	41.0
16	36.62390771909824	38.0	35.0	40.0	31.0	41.0
17	36.42436248379196	38.0	35.0	40.0	30.0	41.0
18	36.50952124427629	38.0	35.0	40.0	31.0	41.0
19	36.50351095882688	38.0	35.0	40.0	30.0	41.0
20	36.55487243411863	38.0	35.0	40.0	31.0	41.0
21	36.22934610349342	38.0	34.0	40.0	30.0	41.0
22	36.38351196106312	38.0	35.0	40.0	30.0	41.0
23	36.4694380586684	38.0	35.0	40.0	30.0	41.0
24	36.36920190675445	38.0	35.0	40.0	30.0	41.0
25	35.88804708004736	38.0	34.0	40.0	29.0	41.0
26	35.97327161228491	38.0	34.0	40.0	30.0	41.0
27	36.048693021303784	38.0	34.0	40.0	30.0	41.0
28	35.901069887185784	38.0	34.0	40.0	29.0	41.0
29	36.08110596769041	38.0	35.0	40.0	30.0	41.0
30	35.6057672431613	38.0	34.0	40.0	29.0	41.0
31	35.767719223517474	38.0	34.0	40.0	29.0	41.0
32	35.85587529675588	38.0	34.0	40.0	29.0	41.0
33	35.88117236584128	38.0	34.0	40.0	29.0	41.0
34	35.85547127027179	38.0	34.0	40.0	29.0	41.0
35	35.9064255870912	38.0	34.0	40.0	29.0	41.0
36	35.68881191157771	38.0	34.0	40.0	29.0	41.0
37	35.72838458310105	38.0	34.0	40.0	29.0	41.0
38	35.62945133829858	38.0	34.0	40.0	29.0	41.0
39	35.55798563043791	38.0	34.0	40.0	29.0	41.0
40	35.440933833616256	38.0	34.0	40.0	28.0	41.0
41	35.36177283062834	38.0	34.0	40.0	28.0	41.0
42	35.56465363341957	38.0	34.0	40.0	29.0	41.0
43	35.517056808002856	38.0	34.0	40.0	28.0	41.0
44	35.42803943799603	38.0	34.0	40.0	28.0	41.0
45	35.533480954379456	38.0	34.0	40.0	29.0	41.0
46	35.41247345639959	37.0	34.0	40.0	28.0	41.0
47	35.39736474508748	37.0	34.0	40.0	28.0	41.0
48	35.3682811022093	37.0	34.0	40.0	28.0	41.0
49	35.34850886039476	37.0	34.0	40.0	28.0	41.0
50	35.166477703375655	37.0	34.0	40.0	28.0	41.0
51	34.01877626955144	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	4.0
11	5.0
12	9.0
13	4.0
14	10.0
15	24.0
16	32.0
17	86.0
18	141.0
19	265.0
20	483.0
21	729.0
22	1019.0
23	1502.0
24	2114.0
25	2795.0
26	3555.0
27	4605.0
28	5923.0
29	7425.0
30	9494.0
31	11953.0
32	15395.0
33	20527.0
34	29492.0
35	30444.0
36	35832.0
37	46788.0
38	58015.0
39	30610.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	23.737025738679428	5.021203560444241	53.707960887730756	17.53380981314558
2	20.533001760177395	4.453374090940411	54.646304567065265	20.36731958181693
3	22.37147886221131	4.22066736405605	50.23051433511021	23.177339438622425
4	20.0415928039438	5.340039964170054	47.63785446277006	26.98051276911609
5	18.294569758774266	6.191940767838239	49.45472084588739	26.05876862750011
6	22.552507782990798	6.928271205126438	52.30984133347532	18.209379678407448
7	85.70435283726815	2.764606027198186	7.424378143733204	4.106662991800455
8	87.28036932405429	3.465858196100048	5.272389017996405	3.9813834618492514
9	81.53160489341845	5.54048721209198	7.502991048777585	5.424916845711994
10	40.68891213520167	28.96869890944169	15.238688824439532	15.103700130917108
11	32.17366248441836	26.481273842260546	22.602306396146403	18.742757277174697
12	29.937736073614253	22.634879073933714	27.488521263068222	19.93886358938381
13	24.884273034207578	24.77371384902564	29.302255657936772	21.039757458830014
14	20.989019249199778	28.16503072480472	28.65142849984027	22.194521526155235
15	18.950408098068817	26.163063836184485	34.38421979040734	20.502308275339352
16	21.86472316355869	23.682215944325776	33.353169258908935	21.099891633206592
17	22.336087395000092	23.558502408498963	29.32981715452604	24.775593041974904
18	22.7062884060059	24.999843400587558	29.526506016549426	22.767362176857112
19	25.80507757934892	26.423018860833235	27.061318065934614	20.710585493883226
20	29.161629385566545	25.504093508641155	26.499126175278594	18.83515093051371
21	27.185344800586307	25.694518394166987	26.412056901962504	20.7080799032842
22	24.60552608006615	25.099753825723646	26.722123738591737	23.572596355618476
23	24.23970985260863	27.045658124690714	26.713667370320028	22.000964652380624
24	23.78713755065991	24.67912780391248	28.81366549112708	22.720069154300532
25	23.470180339883363	25.12731532231291	27.15308532162387	24.24941901617985
26	21.74664720657968	29.11997394185777	26.5573811567059	22.575997694856646
27	22.58789925020201	27.494472040740902	27.792637322024767	22.124991387032317
28	20.38423231836034	28.61165224908076	29.954962008982545	21.049153423576357
29	22.40248554587423	25.989238488377193	29.002837581353397	22.605438384395182
30	22.7219483472498	27.039394148193157	28.212950145011057	22.025707359545986
31	22.87165738554149	27.859975069373537	26.516978508296635	22.751389036788332
32	24.7542955218832	27.63165312603747	26.029954335611333	21.584097016467993
33	24.8739374729866	27.35541176249507	26.21912642583765	21.55152433868068
34	22.041993698439644	27.50637359608627	27.175948835839968	23.27568386963412
35	21.957430015722583	25.527583420507007	27.756619457163794	24.758367106606617
36	22.60731757734445	28.27809550058568	26.088835714688397	23.02575120738147
37	22.99411812606879	30.039838890524486	26.75125122930539	20.214791754101338
38	21.92423094028551	28.251160401646175	26.37165425355324	23.452954404515076
39	22.218324636845963	26.60905896281077	28.478855947332487	22.693760453010782
40	21.791434638537236	25.648478166909918	29.060779363955824	23.49930783059702
41	19.921011256365766	26.871206379233666	27.961138289809135	25.246644074591433
42	22.415013498869353	25.889641262065982	26.422705662008354	25.272639577056307
43	22.718189961351264	26.535770437789317	26.976754383217553	23.769285217641865
44	21.958369612197217	26.702705411449294	28.25617158284422	23.082753393509268
45	21.789868644412845	26.060961019274252	26.933846144209266	25.215324192103633
46	20.845887386230526	28.175366286025692	27.702122861635026	23.276623466108756
47	20.45063046923448	27.89004215656183	27.88878936126232	23.770538012941376
48	21.928302525008927	24.35935180371203	29.033531066191436	24.678814605087602
49	21.075775323690983	25.52351183578359	29.61796007341381	23.782752767111617
50	21.03537267528172	26.014920792017186	28.1653439236296	24.784362609071493
51	19.463114574394115	27.54364425624675	26.900333869947318	26.09290729941181
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44.0
1	181.5
2	319.0
3	444.0
4	569.0
5	437.5
6	306.0
7	347.5
8	389.0
9	427.0
10	465.0
11	447.5
12	430.0
13	479.0
14	528.0
15	557.5
16	587.0
17	574.0
18	561.0
19	662.5
20	764.0
21	856.0
22	948.0
23	1248.0
24	1548.0
25	1727.5
26	2773.0
27	3639.0
28	4161.5
29	4684.0
30	5541.5
31	6399.0
32	7206.5
33	8014.0
34	8341.0
35	8668.0
36	9438.5
37	10209.0
38	11225.0
39	12241.0
40	14622.5
41	17004.0
42	19249.5
43	21495.0
44	22511.0
45	23527.0
46	24875.0
47	26223.0
48	26627.0
49	27031.0
50	26158.0
51	25285.0
52	23370.0
53	21455.0
54	19506.5
55	17558.0
56	16973.5
57	16389.0
58	15781.0
59	15173.0
60	15067.0
61	14961.0
62	12711.5
63	10462.0
64	8814.0
65	7166.0
66	5906.5
67	4647.0
68	3833.0
69	3019.0
70	2517.0
71	2015.0
72	1607.0
73	1199.0
74	955.5
75	561.5
76	411.0
77	282.0
78	153.0
79	140.0
80	127.0
81	78.0
82	29.0
83	27.0
84	25.0
85	13.0
86	1.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	319286.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.529131430660616
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.95699590341803	23.63328975486357
2	8.270999547969776	5.2155486362174726
3	2.7700052788832368	2.620075815045999
4	1.4129999574270813	1.7820264547694524
5	1.0379999435891238	1.63636183232199
6	0.7579999585763669	1.4339448191033748
7	0.7369999597211967	1.6265878016108835
8	0.6279999656782762	1.5840234765056582
9	0.5839999680829827	1.657171057427097
>10	8.685999525289018	53.842925384121045
>50	0.11799999355101361	2.443507552332573
>100	0.03899999786855535	2.3662611845491557
>500	9.999999453475731E-4	0.15827623113173567
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	502	0.15722581008876055	No Hit
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT	435	0.13624148882193393	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	435	0.13624148882193393	No Hit
TCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	377	0.11807595697900942	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGC	361	0.11306477578096126	No Hit
AGCTACCATTACATTTATTATTTTACTTCTACTCACAATTCTAGAATTTGC	349	0.10930638988242516	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15096183359120036	0.0
2	0.0	0.0	0.0	0.6270240474057741	0.0
3	0.0	0.0	0.0	0.8230865117794078	0.0
4	0.0	0.0	0.0	1.1297081613349786	0.0
5	0.0	0.0	0.0	1.6718553271988124	0.0
6	0.0	0.0	0.0	1.939013924819754	0.0
7	0.0	0.0	0.0	2.2290360366567903	0.0
8	0.0	0.0	0.0	2.6740915668084413	0.0
9	0.0	0.0	0.0	2.8497961075650045	0.0
10	0.0	0.0	0.0	3.3415182626234787	0.0
11	0.0	0.0	0.0	4.096953828229236	0.0
12	0.0	0.0	0.0	4.888094059871087	0.0
13	0.0	0.0	0.0	5.1233063773544725	0.0
14	0.0	0.0	0.0	5.208183258896413	0.0
15	0.0	0.0	0.0	5.361963881911515	0.0
16	0.0	0.0	0.0	5.7324780917422	0.0
17	0.0	0.0	0.0	6.280262836453837	0.0
18	0.0	0.0	0.0	6.863439048376691	0.0
19	0.0	0.0	0.0	7.268405128943956	0.0
20	0.0	0.0	0.0	7.6580244670922	0.0
21	0.0	0.0	0.0	8.21802396597408	0.0
22	0.0	0.0	0.0	8.809343347343761	0.0
23	0.0	0.0	0.0	9.381557600395883	0.0
24	0.0	0.0	0.0	9.838201487068021	0.0
25	0.0	0.0	0.0	10.232832006414313	0.0
26	0.0	0.0	0.0	10.626209730461092	0.0
27	0.0	0.0	0.0	10.987641174370314	0.0
28	0.0	0.0	0.0	11.378826506642946	0.0
29	0.0	0.0	0.0	11.784418984859968	0.0
30	0.0	0.0	0.0	12.227282123237474	0.0
31	0.0	0.0	0.0	12.687684395808146	0.0
32	0.0	0.0	0.0	13.126789148287116	0.0
33	0.0	0.0	0.0	13.564014707816817	0.0
34	0.0	0.0	0.0	14.010949430917735	0.0
35	0.0	0.0	0.0	14.47260449878792	0.0
36	0.0	0.0	0.0	14.978733799790783	0.0
37	0.0	0.0	0.0	15.465131574826332	0.0
38	0.0	0.0	0.0	15.954974536935538	0.0
39	0.0	0.0	0.0	16.410052429483283	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCGA	20	7.025624E-4	45.000004	32
GAGTCTA	20	7.025624E-4	45.000004	9
CATTCGA	20	7.025624E-4	45.000004	33
GTGGCGT	40	6.7884685E-9	45.000004	34
ACGGGTA	20	7.025624E-4	45.000004	5
TAATTCG	20	7.025624E-4	45.000004	27
TACGAGT	20	7.025624E-4	45.000004	39
ACGTGTA	20	7.025624E-4	45.000004	25
GAACGGC	20	7.025624E-4	45.000004	10
CCGAAGA	20	7.025624E-4	45.000004	22
CGCGTCG	20	7.025624E-4	45.000004	14
TAGTGGC	20	7.025624E-4	45.000004	45
TTCGAGT	20	7.025624E-4	45.000004	15
TCGTTCA	25	3.8844562E-5	45.0	16
ACCGGTA	25	3.8844562E-5	45.0	41
TCGCAGT	25	3.8844562E-5	45.0	41
CACGCAA	25	3.8844562E-5	45.0	24
TACGGTT	35	1.2082455E-7	45.0	33
ATACTCG	25	3.8844562E-5	45.0	44
GTTACCG	25	3.8844562E-5	45.0	38
>>END_MODULE