SRR2934084.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934084.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	279948
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	3179	1.1355680340634688	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	2249	0.8033634817894751	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	2114	0.7551402403303471	No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	1905	0.6804835183676969	TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	1505	0.5375998399702802	TruSeq Adapter, Index 15 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	1425	0.5090231042907969	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	1232	0.44008172946404334	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGAGACT	1160	0.4143626673525083	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCC	640	0.22861388543586666	No Hit
TTCTTTGGGGATACTTTTGGGATCCACTTGACGACCTGAAAAATGACGAAA	537	0.19182133824853187	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTG	494	0.17646134282080958	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	453	0.16181576578507437	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTA	452	0.16145855658908084	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	411	0.14681297955334563	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTC	410	0.14645577035735208	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	342	0.12216554502979125	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	329	0.1175218254818752	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	310	0.11073485075799791	No Hit
AGCTTTGGGACAAGAAATGGAAGAGAGAATCTCAGGTGCAGAAGATTCCAT	305	0.1089488047780302	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	20	7.024132E-4	45.000004	43
TAGCGCG	20	7.024132E-4	45.000004	1
ATATCAC	20	7.024132E-4	45.000004	30
GTTCGCA	20	7.024132E-4	45.000004	41
AAGTTCG	20	7.024132E-4	45.000004	39
CATAGCG	20	7.024132E-4	45.000004	19
CTACGGC	20	7.024132E-4	45.000004	6
TCGCTAG	20	7.024132E-4	45.000004	1
TAGGGTC	20	7.024132E-4	45.000004	5
CAAGCTA	20	7.024132E-4	45.000004	41
CGTAAGT	20	7.024132E-4	45.000004	36
TGGATAC	20	7.024132E-4	45.000004	15
CACGTAA	20	7.024132E-4	45.000004	34
TAGTAGG	20	7.024132E-4	45.000004	10
GTTGCGA	20	7.024132E-4	45.000004	1
GTGGGAC	35	1.2075361E-7	45.0	5
GATCCAC	75	0.0	45.0	21
TAACGGG	75	0.0	45.0	3
TTCATCG	35	1.2075361E-7	45.0	15
CGCTAGG	30	2.1595988E-6	44.999996	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content