##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934084.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	279948
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.95622044093903	31.0	30.0	31.0	26.0	34.0
2	30.078982525326133	31.0	30.0	33.0	26.0	34.0
3	30.144744738308543	31.0	30.0	33.0	25.0	34.0
4	33.97134825038936	35.0	33.0	37.0	28.0	37.0
5	34.45816008687328	35.0	35.0	37.0	32.0	37.0
6	33.90106376898567	35.0	35.0	37.0	30.0	37.0
7	34.509576778544584	35.0	35.0	37.0	32.0	37.0
8	34.377041450555105	35.0	35.0	37.0	31.0	37.0
9	35.89743095146241	37.0	35.0	39.0	31.0	39.0
10	35.34884335662337	37.0	34.0	39.0	30.0	39.0
11	35.48177161472845	37.0	35.0	39.0	30.0	39.0
12	35.48433280466372	37.0	35.0	39.0	30.0	39.0
13	35.44957277780159	37.0	35.0	39.0	30.0	39.0
14	36.17759726806407	38.0	35.0	40.0	30.0	41.0
15	36.36751825338992	38.0	35.0	40.0	30.0	41.0
16	36.5619114978496	38.0	35.0	40.0	31.0	41.0
17	36.26891779901982	38.0	35.0	40.0	30.0	41.0
18	36.43026204866618	38.0	35.0	40.0	30.0	41.0
19	36.40698629745524	38.0	35.0	40.0	30.0	41.0
20	36.390790432508894	38.0	35.0	40.0	30.0	41.0
21	36.14618071927644	38.0	34.0	40.0	30.0	41.0
22	36.352640490376785	38.0	35.0	40.0	30.0	41.0
23	36.421696172146255	38.0	35.0	40.0	30.0	41.0
24	36.331457985054364	38.0	35.0	40.0	30.0	41.0
25	35.93736336748253	38.0	34.0	40.0	29.0	41.0
26	36.02641562004372	38.0	34.0	40.0	30.0	41.0
27	36.0275586894709	38.0	34.0	40.0	30.0	41.0
28	35.93692400017146	38.0	34.0	40.0	30.0	41.0
29	36.09516052981268	38.0	34.0	40.0	30.0	41.0
30	35.59833611956506	38.0	34.0	40.0	29.0	41.0
31	35.74682083815566	38.0	34.0	40.0	29.0	41.0
32	35.72741366253733	38.0	34.0	40.0	29.0	41.0
33	35.743595239115834	38.0	34.0	40.0	29.0	41.0
34	35.73844071041765	38.0	34.0	40.0	29.0	41.0
35	35.7910040436081	38.0	34.0	40.0	29.0	41.0
36	35.575535456584795	38.0	34.0	40.0	29.0	41.0
37	35.62754511552146	38.0	34.0	40.0	29.0	41.0
38	35.45791004043608	38.0	34.0	40.0	29.0	41.0
39	35.482757512109394	38.0	34.0	40.0	29.0	41.0
40	35.34131338677183	38.0	34.0	40.0	28.0	41.0
41	35.27661565719348	37.0	34.0	40.0	27.0	41.0
42	35.52008230099876	38.0	34.0	40.0	29.0	41.0
43	35.54416534499264	38.0	34.0	40.0	29.0	41.0
44	35.49242002086102	38.0	34.0	40.0	29.0	41.0
45	35.576449912126535	38.0	34.0	40.0	29.0	41.0
46	35.42626844985497	37.0	34.0	40.0	29.0	41.0
47	35.341074056610516	37.0	34.0	40.0	28.0	41.0
48	35.3559732521754	37.0	34.0	40.0	28.0	41.0
49	35.40766856701959	37.0	34.0	40.0	29.0	41.0
50	35.242784374240934	37.0	34.0	40.0	28.0	41.0
51	34.089966708102935	36.0	33.0	39.0	24.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	5.0
11	1.0
12	5.0
13	3.0
14	10.0
15	5.0
16	11.0
17	35.0
18	77.0
19	202.0
20	381.0
21	573.0
22	870.0
23	1206.0
24	1649.0
25	2221.0
26	2849.0
27	3803.0
28	5106.0
29	6531.0
30	8550.0
31	11190.0
32	14672.0
33	18988.0
34	25820.0
35	28055.0
36	33828.0
37	42764.0
38	48991.0
39	21544.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	26.53814279794819	4.491548430422792	53.416705959678225	15.553602811950789
2	18.720976752825525	7.77823024275937	53.076642805092376	20.42415019932273
3	20.351636732536043	7.52711217797591	51.08841642019232	21.032834669295728
4	18.941374826753542	4.717661851486705	51.42455027362225	24.91641304813751
5	19.619715089945274	5.2238272822095535	50.80800720133739	24.34845042650778
6	19.943703830711417	8.81913783988455	52.701930358495154	18.53522797090888
7	78.1355823224313	1.9403603526369182	14.692371440410362	5.2316858845214105
8	78.73747981768042	5.57139182991127	8.656964864903482	7.034163487504823
9	73.39184419963708	4.949847828882507	11.822552759798247	9.83575521168217
10	38.70397359509623	24.493120150885165	19.966922428451	16.835983825567606
11	28.79534770743138	25.341491991369825	26.01447411662166	19.848686184577137
12	24.1094774743881	21.907997199479905	31.78304542272136	22.199479903410634
13	22.740294626144856	25.81122208410133	33.219026390615404	18.22945689913841
14	19.628645319845113	28.32097389515196	30.098804063611812	21.951576721391117
15	17.016374469544342	26.240230328489577	36.35960964179062	20.38378556017546
16	19.45361281380828	25.42222126966437	30.633546230014147	24.49061968651321
17	18.25124666009402	27.909468901367397	29.216140140311776	24.623144298226812
18	20.116950290768283	23.78298826925	33.31797333790561	22.782088102076102
19	21.02211839341592	27.18147655993256	28.758912369440036	23.037492677211482
20	23.512580907882892	26.77211482132396	31.362967408232954	18.35233686256019
21	23.389700944461115	29.484047037306926	28.21416834554989	18.91208367268207
22	20.37556974866761	26.08698758340835	29.461900067155327	24.075542600768713
23	20.39700230042722	27.069670081586576	27.970194464686298	24.563133153299898
24	22.1294669009959	25.037506965579322	28.84249932130253	23.99052681212225
25	19.135696629374028	27.599768528441	27.722291282666784	25.542243559518198
26	18.687041879206138	27.015374283795563	31.281166502350437	23.016417334647862
27	22.08231528712475	26.38561447125895	29.03932158829497	22.492748653321332
28	16.650235043650962	26.813551088059214	33.96130709988998	22.574906768399845
29	21.574363810421936	23.526512066526642	31.601583151156643	23.297540971894783
30	22.13446782973981	27.474030891451267	29.96949433466215	20.42200694414677
31	23.495434866475204	27.761941503422065	26.617800448654748	22.124823181447983
32	22.87782016660237	30.86216011545001	25.720490948318975	20.539528769628646
33	22.30199894266078	27.1514709874691	25.42257847886036	25.123951591009757
34	20.96282166688099	26.653878577450097	29.729806964150484	22.653492791518424
35	20.285910240473232	27.17826167716862	27.522968551302384	25.01285953105577
36	22.18233386200294	26.364896337891324	29.30544243931016	22.147327360795575
37	22.841384828611027	26.2555903239173	30.835012216554503	20.06801263091717
38	20.4977352936974	29.922342720791008	27.320073727978052	22.259848257533545
39	23.27217911897924	26.92928686756112	28.297398088216386	21.501135925243258
40	22.459885407289924	28.471716175861232	27.353294183205453	21.71510423364339
41	20.016574506694102	27.19290725420435	26.605655335990967	26.184862903110577
42	20.591681312243704	26.427765156386187	27.90268192664352	25.077871604726592
43	21.856916284452826	24.597068026919285	28.30847157329218	25.2375441153357
44	21.114635575178248	23.55151671024619	30.053795704916624	25.280052009658938
45	21.60901310243331	23.671896209296012	28.140583251175215	26.57850743709546
46	23.637604126480632	26.216654521554002	29.599782816808833	20.54595853515653
47	18.528083786989015	25.858016488776485	32.716075842656494	22.89782388157801
48	21.133210453369912	24.645291268378415	29.636218154800176	24.585280123451497
49	21.430765713632532	23.10107591409833	32.77251489562347	22.695643476645664
50	22.492034234929346	23.055710346207153	28.628888222098393	25.823367196765112
51	19.59006672667781	23.58259391029763	27.52046808693043	29.30687127609413
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	67.0
1	195.0
2	323.0
3	481.0
4	639.0
5	494.5
6	350.0
7	382.0
8	414.0
9	505.0
10	596.0
11	673.5
12	751.0
13	806.0
14	861.0
15	876.5
16	892.0
17	954.0
18	1016.0
19	982.0
20	948.0
21	1032.5
22	1117.0
23	1206.0
24	1295.0
25	1540.5
26	2367.5
27	2949.0
28	3320.0
29	3691.0
30	4370.5
31	5050.0
32	5548.0
33	6046.0
34	7217.5
35	8389.0
36	8461.0
37	8533.0
38	9787.5
39	11042.0
40	12711.5
41	14381.0
42	16016.0
43	17651.0
44	19521.5
45	21392.0
46	28181.0
47	34970.0
48	34916.5
49	34863.0
50	32598.0
51	30333.0
52	24293.0
53	18253.0
54	15727.0
55	13201.0
56	11595.0
57	9989.0
58	8866.0
59	7743.0
60	7151.0
61	6559.0
62	5730.0
63	4901.0
64	4233.0
65	3565.0
66	2807.5
67	2050.0
68	1662.0
69	1274.0
70	1045.0
71	816.0
72	700.5
73	585.0
74	464.0
75	261.5
76	180.0
77	124.5
78	69.0
79	49.5
80	30.0
81	34.5
82	39.0
83	20.5
84	2.0
85	2.5
86	3.0
87	2.0
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	279948.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.968653059991176
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.6357716036972	26.819451056455556
2	12.419988712762816	9.431404848877955
3	4.390435817992188	5.000968030665119
4	2.30304543305698	3.497741321165504
5	1.5143488954639306	2.8748893911825415
6	1.20221720173237	2.738794070119787
7	0.9968517964663822	2.6494384008582705
8	0.8543004576650162	2.5949310148859746
9	0.7685711436954004	2.626344999620215
>10	4.751982439331946	29.16117116762495
>50	0.0917101707269386	2.3844895024720927
>100	0.060807830590687555	4.184968322037131
>500	0.0019936993636291	0.4454831975253417
>1k	0.0079747974545164	5.589924676509576
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	3179	1.1355680340634688	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGC	2249	0.8033634817894751	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCG	2114	0.7551402403303471	No Hit
CTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGCT	1905	0.6804835183676969	TruSeq Adapter, Index 14 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	1505	0.5375998399702802	TruSeq Adapter, Index 15 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	1425	0.5090231042907969	No Hit
TCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTGC	1232	0.44008172946404334	TruSeq Adapter, Index 15 (95% over 22bp)
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCTGTGAGACT	1160	0.4143626673525083	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCC	640	0.22861388543586666	No Hit
TTCTTTGGGGATACTTTTGGGATCCACTTGACGACCTGAAAAATGACGAAA	537	0.19182133824853187	No Hit
CGCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCTG	494	0.17646134282080958	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCT	453	0.16181576578507437	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTA	452	0.16145855658908084	No Hit
TTCCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	411	0.14681297955334563	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTC	410	0.14645577035735208	No Hit
GAACTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTCT	342	0.12216554502979125	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	329	0.1175218254818752	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTGTGAGACTCGTATGCCGTCTTC	310	0.11073485075799791	No Hit
AGCTTTGGGACAAGAAATGGAAGAGAGAATCTCAGGTGCAGAAGATTCCAT	305	0.1089488047780302	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.7304928058067927	0.0
2	0.0	0.0	0.0	3.1105776787117607	0.0
3	0.0	0.0	0.0	3.853215597182334	0.0
4	0.0	0.0	0.0	5.055938960092589	0.0
5	0.0	0.0	0.0	7.139540200322917	0.0
6	0.0	0.0	0.0	8.000414362667353	0.0
7	0.0	0.0	0.0	9.002028948233244	0.0
8	0.0	0.0	0.0	10.388715047080172	0.0
9	0.0	0.0	0.0	10.85844513981168	0.0
10	0.0	0.0	0.0	12.723791561289953	0.0
11	0.0	0.0	0.0	14.76488490719705	0.0
12	0.0	0.0	0.0	17.379656221869777	0.0
13	0.0	0.0	0.0	17.97619557917899	0.0
14	0.0	0.0	0.0	18.20909597496678	0.0
15	0.0	0.0	0.0	18.69347164473402	0.0
16	0.0	0.0	0.0	19.530770000142883	0.0
17	0.0	0.0	0.0	20.792790089588067	0.0
18	0.0	0.0	0.0	22.19126409190278	0.0
19	0.0	0.0	0.0	23.564376241301957	0.0
20	0.0	0.0	0.0	24.542415019932275	0.0
21	0.0	0.0	0.0	25.846943003700687	0.0
22	0.0	0.0	0.0	27.138611456413333	0.0
23	0.0	0.0	0.0	28.497078028776773	0.0
24	0.0	0.0	0.0	29.562633060425508	0.0
25	0.0	0.0	0.0	30.441010473373627	0.0
26	0.0	0.0	0.0	31.26759255290268	0.0
27	0.0	0.0	0.0	32.03237744152486	0.0
28	0.0	0.0	0.0	32.87360509808965	0.0
29	0.0	0.0	0.0	33.715547173046424	0.0
30	0.0	0.0	0.0	34.66643805278123	0.0
31	0.0	0.0	0.0	35.56267592552903	0.0
32	0.0	0.0	0.0	36.40390358209382	0.0
33	0.0	0.0	0.0	37.222984268507005	0.0
34	0.0	0.0	0.0	38.009201708888796	0.0
35	0.0	0.0	0.0	38.875434009173134	0.0
36	0.0	0.0	0.0	39.66129424035893	0.0
37	0.0	0.0	0.0	40.46180004858045	0.0
38	0.0	0.0	0.0	41.31338677182905	0.0
39	0.0	0.0	0.0	42.1681883778416	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCATA	20	7.024132E-4	45.000004	43
TAGCGCG	20	7.024132E-4	45.000004	1
ATATCAC	20	7.024132E-4	45.000004	30
GTTCGCA	20	7.024132E-4	45.000004	41
AAGTTCG	20	7.024132E-4	45.000004	39
CATAGCG	20	7.024132E-4	45.000004	19
CTACGGC	20	7.024132E-4	45.000004	6
TCGCTAG	20	7.024132E-4	45.000004	1
TAGGGTC	20	7.024132E-4	45.000004	5
CAAGCTA	20	7.024132E-4	45.000004	41
CGTAAGT	20	7.024132E-4	45.000004	36
TGGATAC	20	7.024132E-4	45.000004	15
CACGTAA	20	7.024132E-4	45.000004	34
TAGTAGG	20	7.024132E-4	45.000004	10
GTTGCGA	20	7.024132E-4	45.000004	1
GTGGGAC	35	1.2075361E-7	45.0	5
GATCCAC	75	0.0	45.0	21
TAACGGG	75	0.0	45.0	3
TTCATCG	35	1.2075361E-7	45.0	15
CGCTAGG	30	2.1595988E-6	44.999996	2
>>END_MODULE