##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934082.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	556167
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.017901098051485	31.0	30.0	31.0	26.0	34.0
2	30.27843615316982	31.0	30.0	33.0	26.0	34.0
3	30.324550000269703	31.0	30.0	34.0	26.0	34.0
4	33.9615583089252	35.0	33.0	37.0	28.0	37.0
5	34.3934375825966	35.0	35.0	37.0	32.0	37.0
6	33.8407043208245	35.0	35.0	37.0	29.0	37.0
7	34.32166237838635	35.0	35.0	37.0	32.0	37.0
8	34.382937139384396	35.0	35.0	37.0	31.0	37.0
9	35.780790661797624	37.0	35.0	39.0	30.0	39.0
10	35.26609993041659	37.0	34.0	39.0	30.0	39.0
11	35.41626166241435	37.0	35.0	39.0	30.0	39.0
12	35.460661995407854	37.0	35.0	39.0	30.0	39.0
13	35.4760170955846	37.0	35.0	39.0	30.0	39.0
14	36.18098520768043	38.0	35.0	40.0	30.0	41.0
15	36.43630060755133	38.0	35.0	40.0	30.0	41.0
16	36.569044909172966	38.0	35.0	40.0	31.0	41.0
17	36.323214070593906	38.0	35.0	40.0	30.0	41.0
18	36.45199733173669	38.0	35.0	40.0	30.0	41.0
19	36.44073632560004	38.0	35.0	40.0	30.0	41.0
20	36.451688072107835	38.0	35.0	40.0	30.0	41.0
21	36.16399390830452	38.0	34.0	40.0	30.0	41.0
22	36.32710858429213	38.0	35.0	40.0	30.0	41.0
23	36.382901178962435	38.0	35.0	40.0	30.0	41.0
24	36.30071902863708	38.0	35.0	40.0	30.0	41.0
25	35.87113942395	38.0	34.0	40.0	29.0	41.0
26	35.99918010237932	38.0	34.0	40.0	30.0	41.0
27	36.01239375942837	38.0	34.0	40.0	30.0	41.0
28	35.90189457483094	38.0	34.0	40.0	29.0	41.0
29	36.008493851666856	38.0	34.0	40.0	30.0	41.0
30	35.5451635929496	38.0	34.0	40.0	28.0	41.0
31	35.737620175235136	38.0	34.0	40.0	29.0	41.0
32	35.72628005617018	38.0	34.0	40.0	29.0	41.0
33	35.74809364813087	38.0	34.0	40.0	29.0	41.0
34	35.715659145544414	38.0	34.0	40.0	29.0	41.0
35	35.694314837090296	38.0	34.0	40.0	29.0	41.0
36	35.49544291552717	38.0	34.0	40.0	28.0	41.0
37	35.578308313869755	38.0	34.0	40.0	28.0	41.0
38	35.446004527417124	38.0	34.0	40.0	28.0	41.0
39	35.33046009561876	38.0	34.0	40.0	27.0	41.0
40	35.279500581659825	38.0	34.0	40.0	27.0	41.0
41	35.161415546050016	38.0	34.0	40.0	27.0	41.0
42	35.381367466965855	38.0	34.0	40.0	28.0	41.0
43	35.37626288506869	38.0	34.0	40.0	28.0	41.0
44	35.348798112797056	38.0	34.0	40.0	28.0	41.0
45	35.394448070453656	38.0	34.0	40.0	28.0	41.0
46	35.298365419019824	37.0	34.0	40.0	28.0	41.0
47	35.23136575884581	37.0	34.0	40.0	28.0	41.0
48	35.211188366084286	37.0	34.0	40.0	28.0	41.0
49	35.191800304584774	37.0	34.0	40.0	28.0	41.0
50	34.99860293760687	37.0	34.0	40.0	27.0	41.0
51	33.90373035437198	36.0	32.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	5.0
10	5.0
11	12.0
12	20.0
13	18.0
14	32.0
15	48.0
16	79.0
17	139.0
18	257.0
19	516.0
20	908.0
21	1498.0
22	2306.0
23	3130.0
24	4045.0
25	5238.0
26	6568.0
27	8393.0
28	10318.0
29	13396.0
30	17153.0
31	21457.0
32	27621.0
33	36239.0
34	48524.0
35	52173.0
36	63983.0
37	82589.0
38	99603.0
39	49893.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	25.60202241413101	4.6113847099881875	52.25948321277601	17.52710966310479
2	20.134959463614347	5.782256048992479	53.395652744589306	20.687131742803867
3	22.307508356303053	5.824509544794998	50.31006873834658	21.55791336055537
4	19.71835797521248	5.248423585002347	47.73781975557701	27.29539868420816
5	19.63313177516825	6.529333815202988	48.37324041160299	25.464293998025774
6	22.162587855805903	8.476770466424654	51.13050576535465	18.230135912414795
7	83.40228744244085	2.798260234785595	9.670656475483083	4.128795847290472
8	84.89410554743449	4.804671978020989	5.91584901657236	4.385373457972156
9	79.02464547519	6.049262182042444	8.062506405450161	6.863585937317389
10	40.7084562730259	29.386137616938797	15.504875334207172	14.400530775828123
11	29.94370395942226	25.89635846787026	24.919313803228167	19.240623769479313
12	26.349100180341516	22.436965875357583	29.523506428824437	21.690427515476465
13	24.14400710577938	23.84823263516174	31.37187211754743	20.63588814151145
14	20.3854238025629	26.355393254184445	31.2861065111738	21.97307643207885
15	19.70703044229521	24.88748882979393	33.47987205281866	21.925608675092192
16	22.719075385630575	24.425936813942574	30.08646683460184	22.76852096582501
17	22.852668353210458	25.388057903471438	28.413228400822057	23.34604534249605
18	23.62132237259672	23.9046904976383	29.823596150077226	22.650390979687756
19	23.518115961572693	25.288807138862968	28.62323726506607	22.56983963449827
20	25.90804560500713	26.084611276828724	28.86003664366998	19.147306474494172
21	26.074722160789836	26.37247445461525	28.036003574465944	19.516799810128973
22	24.212871313831997	23.707986989519338	29.8613545931348	22.21778710351387
23	24.098696974110297	25.137054158193493	29.050986484275406	21.713262383420805
24	22.763126902531074	25.56498317951263	29.04073776401692	22.631152153939375
25	22.970618537237915	26.8868882907472	27.050328408553547	23.092164763461334
26	21.5381351284776	26.36905821452909	28.00184117360433	24.09096548338898
27	22.03600717050814	26.11230080173761	28.246192240819756	23.6054997869345
28	19.885034530995185	26.97265389712083	29.606934607770686	23.535376964113297
29	21.629654402364757	24.508286180230037	29.991890924848114	23.87016849255709
30	21.829234744240487	25.869567953510362	29.68173228544664	22.619465016802508
31	23.64181981311369	25.51553759931819	27.193450887952718	23.649191699615404
32	23.800585076065282	26.503190588438365	27.564562442575703	22.13166189292065
33	23.960788755895262	25.134357126546526	27.549099461133075	23.355754656425137
34	21.71811704038535	25.139571387730662	29.24751019028457	23.89480138159941
35	21.44373902083367	26.003160921090245	28.16366307242249	24.38943698565359
36	23.057642758380126	26.902710876409426	26.69899508600834	23.34065127920211
37	21.87112863582341	26.4034004175005	28.4623143767969	23.26315656987919
38	22.926027614008024	26.643256431970975	27.244874291354936	23.18584166266607
39	21.981167527019764	25.953895143005607	29.42012021569061	22.64481711428402
40	23.30576966990131	24.375412421089347	28.525784521555575	23.793033387453768
41	19.99399460953275	25.316316861662052	29.02365656358612	25.666031965219076
42	20.997290382205342	25.004000596943005	29.28454942490295	24.714159595948697
43	21.382066897172972	24.27957789656704	29.349457986540013	24.988897219719977
44	22.104691576450957	24.243977078827044	29.30234983377295	24.34898151094905
45	22.089048792898534	24.330282091530062	27.696357389057603	25.884311726513797
46	22.54179050536979	25.088327786438246	28.526683532104563	23.843198176087398
47	19.933221496421037	26.001542702102064	30.23318535619697	23.832050445279926
48	20.478021889108845	25.520212454172935	29.987755476322757	24.014010180395456
49	21.78949847797514	24.089167462291	30.57750639646006	23.5438276632738
50	21.30187515620308	24.712721179070314	29.584099739826346	24.401303924900255
51	20.563607693372674	24.879397734853022	28.5148165928579	26.042177978916403
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	109.0
1	320.0
2	531.0
3	979.0
4	1427.0
5	1061.5
6	696.0
7	690.0
8	684.0
9	762.5
10	841.0
11	909.5
12	978.0
13	981.5
14	985.0
15	999.5
16	1014.0
17	1047.0
18	1080.0
19	1151.5
20	1223.0
21	1449.5
22	1676.0
23	2236.0
24	2796.0
25	3154.5
26	4643.0
27	5773.0
28	7336.0
29	8899.0
30	9908.5
31	10918.0
32	12841.5
33	14765.0
34	16050.5
35	17336.0
36	17641.5
37	17947.0
38	21325.0
39	24703.0
40	26336.0
41	27969.0
42	28975.5
43	29982.0
44	32737.0
45	35492.0
46	40887.0
47	46282.0
48	46530.0
49	46778.0
50	47026.5
51	47275.0
52	43351.0
53	39427.0
54	37017.0
55	34607.0
56	32141.5
57	29676.0
58	28323.5
59	26971.0
60	24606.5
61	22242.0
62	19738.0
63	17234.0
64	14865.0
65	12496.0
66	10456.0
67	8416.0
68	6947.5
69	5479.0
70	4383.5
71	3288.0
72	2711.5
73	2135.0
74	1713.5
75	984.5
76	677.0
77	491.5
78	306.0
79	241.0
80	176.0
81	111.5
82	47.0
83	33.5
84	20.0
85	12.0
86	4.0
87	2.5
88	1.0
89	1.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	556167.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.08359072204529
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.36933824226021	23.11666072191768
2	8.819672479676834	5.482941793215221
3	3.0049917242599444	2.8021779864008787
4	1.5252328944914366	1.8963886019268923
5	1.0151918975375889	1.5777904723697476
6	0.8239743920892826	1.5367249661489601
7	0.6800291871503137	1.4796424252697846
8	0.6172188289908083	1.534830197303228
9	0.5480248283647061	1.5331121523366877
>10	8.427821766866527	52.4763610092659
>50	0.11742422182044543	2.362258287683521
>100	0.04520832540087148	2.300756352968645
>500	0.003522726654613362	0.8564646279433531
>1k	0.0023484844364089082	1.0438904052495162
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGC	1787	0.32130637020894803	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCG	1697	0.30512418032713196	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTC	1191	0.21414431276936602	No Hit
CCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	1045	0.1878932047388644	No Hit
GCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	938	0.16865437899048308	No Hit
CTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGCT	877	0.15768645029280773	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCCGTGTATT	784	0.14096485408159778	No Hit
TCTGTCTCTTATACACATCTGACGCCCGTGTATTCGTATGCCGTCTTCTGC	744	0.13377276968967955	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	711	0.12783930006634697	No Hit
CGGTCGGCGTCCCCCAACTTCTTAGAGGGACAAGTGGCGTTCAGCCACCCG	639	0.11489354816089412	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17476765072361358	0.0
2	0.0	0.0	0.0	0.7352108269638435	0.0
3	0.0	0.0	0.0	0.9524117755997749	0.0
4	0.0	0.0	0.0	1.3334124462616443	0.0
5	0.0	0.0	0.0	2.0875024947542733	0.0
6	0.0	0.0	0.0	2.4338013582251374	0.0
7	0.0	0.0	0.0	2.832063031427611	0.0
8	0.0	0.0	0.0	3.366075297527541	0.0
9	0.0	0.0	0.0	3.571948713246201	0.0
10	1.7980210979795638E-4	0.0	0.0	4.332511637691557	0.0
11	1.7980210979795638E-4	0.0	0.0	5.1234611186927665	0.0
12	1.7980210979795638E-4	0.0	0.0	6.282285716340596	0.0
13	1.7980210979795638E-4	0.0	0.0	6.554326308464904	0.0
14	1.7980210979795638E-4	0.0	0.0	6.672456294602161	0.0
15	1.7980210979795638E-4	0.0	0.0	6.9153689449392	0.0
16	1.7980210979795638E-4	0.0	0.0	7.290076541758141	0.0
17	1.7980210979795638E-4	0.0	0.0	7.817975536124941	0.0
18	1.7980210979795638E-4	0.0	0.0	8.37769950392598	0.0
19	1.7980210979795638E-4	0.0	0.0	8.942278128691562	0.0
20	1.7980210979795638E-4	0.0	0.0	9.36822932680292	0.0
21	1.7980210979795638E-4	0.0	0.0	9.966610748210519	0.0
22	1.7980210979795638E-4	0.0	0.0	10.581174359499935	0.0
23	1.7980210979795638E-4	0.0	0.0	11.171464685966626	0.0
24	1.7980210979795638E-4	0.0	0.0	11.653693944444743	0.0
25	1.7980210979795638E-4	0.0	0.0	12.112189324429533	0.0
26	1.7980210979795638E-4	0.0	0.0	12.513507633498572	0.0
27	1.7980210979795638E-4	0.0	0.0	12.93855982106094	0.0
28	1.7980210979795638E-4	0.0	0.0	13.373680926771994	0.0
29	1.7980210979795638E-4	0.0	0.0	13.812038470459413	0.0
30	1.7980210979795638E-4	0.0	0.0	14.347848757657323	0.0
31	1.7980210979795638E-4	0.0	0.0	14.803467303885343	0.0
32	1.7980210979795638E-4	0.0	0.0	15.261063673321143	0.0
33	1.7980210979795638E-4	0.0	0.0	15.754800266826331	0.0
34	1.7980210979795638E-4	0.0	0.0	16.215633074238493	0.0
35	1.7980210979795638E-4	0.0	0.0	16.749465538228627	0.0
36	1.7980210979795638E-4	0.0	0.0	17.234931234683106	0.0
37	1.7980210979795638E-4	0.0	0.0	17.73532050625082	0.0
38	1.7980210979795638E-4	0.0	0.0	18.280480503158223	0.0
39	5.394063293938691E-4	0.0	0.0	18.947366528398845	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTTA	20	7.030142E-4	45.000004	38
CGTATTA	20	7.030142E-4	45.000004	27
GATCGAC	20	7.030142E-4	45.000004	10
CGCATCG	20	7.030142E-4	45.000004	21
TATGCGT	20	7.030142E-4	45.000004	23
TATGCGG	20	7.030142E-4	45.000004	2
TCGTAGA	20	7.030142E-4	45.000004	19
TCGTAAT	20	7.030142E-4	45.000004	32
TCGTAAA	20	7.030142E-4	45.000004	14
CGTCGTA	20	7.030142E-4	45.000004	30
TAACGAA	35	1.2104101E-7	45.0	19
CTACGAA	50	2.1827873E-11	45.0	11
ACGAACG	35	1.2104101E-7	45.0	21
CGAACAC	30	2.1634733E-6	44.999996	34
CCGATCG	30	2.1634733E-6	44.999996	40
CCGATAT	30	2.1634733E-6	44.999996	12
CGATCGA	30	2.1634733E-6	44.999996	41
CCCGATC	95	0.0	42.63158	41
CGGGTAC	65	0.0	41.538464	6
TCGGGTC	65	0.0	41.538464	5
>>END_MODULE