FastQCFastQC Report
Sat 14 Jan 2017
SRR2934081.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2934081.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences651904
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128131.9654734439426664No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC29720.45589534655409386RNA PCR Primer, Index 4 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCT20420.31323630473198505RNA PCR Primer, Index 4 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTT20410.3130829079128215RNA PCR Primer, Index 4 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCG15700.2408330060867858No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC15600.2392990378951502No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC13880.21291478499901825TruSeq Adapter, Index 4 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCC12780.19604113489102692No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTG11160.17119085018653052TruSeq Adapter, Index 4 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC11060.16965688199489495RNA PCR Primer, Index 4 (96% over 26bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGT10560.16198704103671707No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT9720.1491017082269782TruSeq Adapter, Index 4 (95% over 24bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTC9110.1397445022580012No Hit
CGTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT8020.12302424896917337TruSeq Adapter, Index 4 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCT7220.11075250343608875TruSeq Adapter, Index 4 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC6830.10477002748871RNA PCR Primer, Index 48 (96% over 25bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAGCGG351.2108467E-745.02
CGTTTTT72800.043.980081
CATACGA800.042.187518
CGCTCGA451.9261279E-840.041
CGATGAA3900.039.80769319
GGCGATC2800.039.3758
TTTCGCG1950.039.230771
CGTTTTC2900.038.7931021
CTTCGCG700.038.5714261
ACACGCG700.038.57142636
GGATCGA356.2441286E-638.5714268
GGCGATA700.038.5714268
GGACCGA3100.038.467748
CTTGTCG1000.038.251
GTTTTTT84600.038.0319182
TTGGGAC17350.037.737755
GACCGAT3050.037.622959
TTGGGAT28700.037.5522655
ACATACG900.037.517
CCTCGCG301.13938004E-437.4999961
Produced by FastQC (version 0.11.5)