##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934081.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	651904
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.02951814991164	31.0	30.0	31.0	26.0	34.0
2	30.343331226683684	31.0	30.0	33.0	27.0	34.0
3	30.328324415864913	31.0	30.0	33.0	26.0	34.0
4	33.99181014382486	35.0	33.0	37.0	28.0	37.0
5	34.47875914245042	35.0	35.0	37.0	32.0	37.0
6	34.14397672049872	35.0	35.0	37.0	30.0	37.0
7	34.485714154231296	35.0	35.0	37.0	32.0	37.0
8	34.69985304584724	35.0	35.0	37.0	32.0	37.0
9	36.0123791233065	37.0	35.0	39.0	31.0	39.0
10	35.59898389946986	37.0	35.0	39.0	30.0	39.0
11	35.707687021401924	37.0	35.0	39.0	30.0	39.0
12	35.85752810229727	37.0	35.0	39.0	30.0	39.0
13	36.001205698998625	37.0	35.0	39.0	31.0	39.0
14	36.8470510995484	38.0	36.0	40.0	31.0	41.0
15	37.01546546730807	39.0	36.0	40.0	31.0	41.0
16	37.100401286078935	39.0	36.0	40.0	32.0	41.0
17	36.7452124852739	38.0	35.0	40.0	31.0	41.0
18	36.737295675436876	38.0	36.0	40.0	31.0	41.0
19	36.60694519438445	38.0	35.0	40.0	31.0	41.0
20	36.440626227174555	38.0	35.0	40.0	31.0	41.0
21	36.12499693206362	38.0	34.0	40.0	30.0	41.0
22	36.24190218191636	38.0	35.0	40.0	30.0	41.0
23	36.33392800166896	38.0	35.0	40.0	30.0	41.0
24	36.16198243913214	38.0	35.0	40.0	30.0	41.0
25	35.71145751521696	38.0	34.0	40.0	29.0	41.0
26	35.793530949342234	38.0	34.0	40.0	29.0	41.0
27	35.88480052277636	38.0	34.0	40.0	30.0	41.0
28	35.72199434272531	38.0	34.0	40.0	29.0	41.0
29	35.74353892597683	38.0	34.0	40.0	29.0	41.0
30	35.19069985764775	38.0	34.0	40.0	27.0	41.0
31	35.259598038975064	38.0	34.0	40.0	27.0	41.0
32	35.04230377478893	38.0	34.0	40.0	25.0	41.0
33	34.735869085018656	38.0	33.0	40.0	24.0	41.0
34	34.43323250049087	38.0	33.0	40.0	22.0	41.0
35	34.31290496760259	38.0	33.0	40.0	21.0	41.0
36	34.0444728058119	38.0	33.0	40.0	20.0	41.0
37	34.11296755350481	38.0	33.0	40.0	21.0	41.0
38	34.031470891419595	38.0	33.0	40.0	20.0	41.0
39	33.886438800805024	37.0	33.0	40.0	19.0	41.0
40	33.82543595376006	37.0	33.0	40.0	19.0	41.0
41	33.68508093216179	37.0	33.0	40.0	18.0	41.0
42	33.83016977959945	37.0	33.0	40.0	18.0	41.0
43	33.78511099793835	37.0	33.0	40.0	18.0	41.0
44	33.70649819605341	37.0	33.0	40.0	18.0	41.0
45	33.782263952974674	37.0	33.0	40.0	19.0	41.0
46	33.69550424602395	37.0	33.0	40.0	19.0	41.0
47	33.64131681965443	37.0	33.0	40.0	19.0	41.0
48	33.57510461663067	37.0	32.0	40.0	19.0	41.0
49	33.580603892597686	37.0	32.0	40.0	19.0	41.0
50	33.43324323826821	37.0	32.0	40.0	18.0	41.0
51	32.443358224523855	36.0	31.0	39.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	3.0
10	3.0
11	10.0
12	13.0
13	16.0
14	46.0
15	69.0
16	145.0
17	349.0
18	694.0
19	1265.0
20	2083.0
21	3290.0
22	4568.0
23	6203.0
24	8483.0
25	11787.0
26	14853.0
27	15756.0
28	16262.0
29	17462.0
30	19901.0
31	23004.0
32	28769.0
33	35910.0
34	48031.0
35	57253.0
36	68418.0
37	89186.0
38	114259.0
39	63813.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	21.365569163557822	4.036637296289024	46.70641689573925	27.891376644413903
2	27.72524788925977	5.44589387394463	48.58614151776949	18.242716719026113
3	18.009093363440016	5.558793932849008	57.90423129785981	18.52788140585117
4	16.841896966424503	4.129288975063813	55.39573311407815	23.633080944433534
5	17.177682603573533	4.9361562438641275	56.23051860396623	21.655642548596113
6	19.48246981150599	6.527494845866875	57.910213773807186	16.07982156881995
7	75.60131553112114	1.5985482525034362	19.01767744944041	3.782458766935009
8	77.52414465933634	2.996606862360102	16.05221014137051	3.4270383369330455
9	72.236096112311	4.68059714313764	17.14562266836835	5.937684076182996
10	33.19062315923817	33.005933388965246	22.593050510504614	11.21039294129197
11	21.865182603573533	21.72804584724131	37.733009768309444	18.67376178087571
12	19.56484390339682	19.55717406243864	40.17401335165914	20.7039686825054
13	19.848014431572746	19.73603475358335	41.30961000392696	19.106340810916944
14	17.33583472413116	23.35420552719419	39.47605782446495	19.833901924209698
15	16.221406832907913	24.497778814058513	40.453809149813466	18.827005203220107
16	19.21954766345965	24.87406121146672	37.70723910268997	18.199152022383664
17	19.010314402120557	24.869612703710974	37.71153421362655	18.40853868054192
18	18.463147948164146	24.385799136069114	37.492943746318474	19.658109169448263
19	18.828539171411744	25.267063862163752	36.05331459846849	19.851082367956018
20	20.960601560966033	26.103383320243474	35.635921853524444	17.30009326526605
21	20.946489053602985	25.996465737286474	35.74575397604555	17.311291233064992
22	20.507160563518557	23.37031219320636	36.86892548596112	19.25360175731396
23	19.35653102297271	24.39776408796387	36.75648561751424	19.489219271549185
24	18.755522285489885	24.844609022187317	36.67687266836835	19.722996023954448
25	19.27032201060279	26.580907618299626	34.622582466129984	19.5261879049676
26	19.349167975652858	26.184223443942667	34.66998208325152	19.796626497152957
27	19.457159336344002	26.08037379736894	34.832275917926566	19.630190948360497
28	18.86642818574514	25.935720596897703	35.36962497545651	19.828226241900648
29	20.198986353818967	24.243140094247007	35.13569482623208	20.422178725701944
30	20.05816807382682	25.90841596308659	34.59128951502062	19.44212644806597
31	20.992201305713724	25.849664981346947	32.841491998821915	20.316641714117416
32	21.529396966424503	26.770966277243275	32.24784630865894	19.45179044767328
33	21.17781145690163	27.138351659139996	31.500343608874925	20.183493275083446
34	20.108482230512468	27.85041969369723	31.95761952680149	20.083478548988808
35	19.842798939721185	28.504043540153152	31.34004393284901	20.313113587276654
36	19.843412526997838	29.156440212055763	30.77753779697624	20.222609463970155
37	19.324931278225016	28.83860200274887	31.604346652267818	20.2321200667583
38	19.429701305713724	28.23912723345769	31.193243176909487	21.137928283919106
39	19.51238219124288	28.100609905752993	31.369189328490084	21.01781857451404
40	19.70029329471824	27.89337080306303	31.932309051639507	20.47402685057923
41	18.732359365796192	28.28545307284508	31.827078833693307	21.15510872766542
42	19.496582318869034	27.519849548399765	32.04582269782053	20.937745434910664
43	20.213252258001177	27.474597486746514	31.15688813076772	21.155262124484587
44	20.203741655213037	27.312150255252305	31.24064279403102	21.243465295503633
45	20.10986280188494	26.674172884351073	30.73903519536619	22.476929118397802
46	20.44181351855488	27.49960116827018	30.791650304339292	21.26693500883566
47	19.074127478892596	27.57093068918123	32.26027145101119	21.09467038091498
48	19.258970645984686	26.802259228352643	32.315340909090914	21.623429216571765
49	19.30575667582957	26.204318427253092	33.05471357745926	21.43521131945808
50	18.912600628313374	26.17808757117612	33.092602591792655	21.81670920871785
51	18.63602616336148	25.404660808953466	33.09060843314353	22.868704594541526
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	336.0
1	453.5
2	571.0
3	10651.5
4	20732.0
5	14302.0
6	7872.0
7	7538.0
8	7204.0
9	7265.5
10	7327.0
11	7279.5
12	7232.0
13	7103.5
14	6975.0
15	6505.5
16	6036.0
17	5771.0
18	5506.0
19	5307.0
20	5108.0
21	5208.5
22	5309.0
23	5631.5
24	5954.0
25	6601.0
26	8287.5
27	9327.0
28	10514.5
29	11702.0
30	13252.5
31	14803.0
32	16435.5
33	18068.0
34	19876.5
35	21685.0
36	23416.0
37	25147.0
38	26639.0
39	28131.0
40	29827.5
41	31524.0
42	33129.0
43	34734.0
44	37197.0
45	39660.0
46	48225.0
47	56790.0
48	54575.5
49	52361.0
50	49938.0
51	47515.0
52	42327.0
53	37139.0
54	33650.0
55	30161.0
56	27982.5
57	25804.0
58	23754.0
59	21704.0
60	19679.0
61	17654.0
62	15321.0
63	12988.0
64	10915.0
65	8842.0
66	7103.0
67	5364.0
68	4239.0
69	3114.0
70	2470.5
71	1827.0
72	1481.0
73	1135.0
74	932.5
75	524.0
76	318.0
77	241.0
78	164.0
79	110.0
80	56.0
81	43.5
82	31.0
83	21.5
84	12.0
85	8.0
86	4.0
87	2.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	651904.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.66819182253387
#Duplication Level	Percentage of deduplicated	Percentage of total
1	69.80307642823415	38.16007971981698
2	13.917387760845346	15.21676847556957
3	7.309376419583501	11.987711766266901
4	4.019523131322043	8.789602463129018
5	2.137573086871253	5.842862777388177
6	1.1492616691103135	3.7696834428724846
7	0.5880993277597674	2.250522880247193
8	0.33829666681103665	1.4795253659319665
9	0.19244015700660447	0.9468319875836031
>10	0.5106237527369225	4.511385471609115
>50	0.02156994857589631	0.8190712554452015
>100	0.007379176216077167	0.8262069715899492
>500	0.0022705157587630457	0.9092155593397565
>1k	0.0028381446984538073	2.502514975527463
>5k	0.0	0.0
>10k+	2.838144698453807E-4	1.9880168876826452
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	12813	1.9654734439426664	No Hit
GAATCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC	2972	0.45589534655409386	RNA PCR Primer, Index 4 (95% over 23bp)
CGTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCT	2042	0.31323630473198505	RNA PCR Primer, Index 4 (95% over 21bp)
CGTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTT	2041	0.3130829079128215	RNA PCR Primer, Index 4 (95% over 22bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCG	1570	0.2408330060867858	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGC	1560	0.2392990378951502	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTC	1388	0.21291478499901825	TruSeq Adapter, Index 4 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCC	1278	0.19604113489102692	No Hit
CGCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTG	1116	0.17119085018653052	TruSeq Adapter, Index 4 (96% over 25bp)
CCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	1106	0.16965688199489495	RNA PCR Primer, Index 4 (96% over 26bp)
CGTTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGT	1056	0.16198704103671707	No Hit
GAACTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT	972	0.1491017082269782	TruSeq Adapter, Index 4 (95% over 24bp)
CGTTTTTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTC	911	0.1397445022580012	No Hit
CGTCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCT	802	0.12302424896917337	TruSeq Adapter, Index 4 (95% over 24bp)
CTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGCT	722	0.11075250343608875	TruSeq Adapter, Index 4 (96% over 27bp)
GCTGTCTCTTATACACATCTGACGCGAGCAATCTCGTATGCCGTCTTCTGC	683	0.10477002748871	RNA PCR Primer, Index 48 (96% over 25bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12624558217160808	0.0
2	0.0	0.0	0.0	0.48657471038680544	0.0
3	0.0	0.0	0.0	0.758700667582957	0.0
4	0.0	0.0	0.0	1.270432456312586	0.0
5	0.0	0.0	0.0	2.4856420577262908	0.0
6	0.0	0.0	0.0	3.615256234046731	0.0
7	0.0	0.0	0.0	4.653906096603181	0.0
8	0.0	0.0	0.0	5.7017597683094445	0.0
9	0.0	0.0	0.0	6.477947673277047	0.0
10	0.0	0.0	0.0	7.368109414883173	0.0
11	0.0	0.0	0.0	8.10656170233654	0.0
12	0.0	0.0	0.0	8.95852763597094	0.0
13	0.0	0.0	0.0	9.269002797957981	0.0
14	0.0	0.0	0.0	9.467191488317297	0.0
15	0.0	0.0	0.0	9.650040496760258	0.0
16	0.0	0.0	0.0	9.838871981150598	0.0
17	0.0	0.0	0.0	10.066512860789318	0.0
18	0.0	0.0	0.0	10.313941930100137	0.0
19	0.0	0.0	0.0	10.610304584724132	0.0
20	0.0	0.0	0.0	10.821838798350678	0.0
21	0.0	0.0	0.0	11.044877773414491	0.0
22	0.0	0.0	0.0	11.293840810916945	0.0
23	0.0	0.0	0.0	11.536054388376202	0.0
24	0.0	0.0	0.0	11.752343903396818	0.0
25	0.0	0.0	0.0	11.950379196936971	0.0
26	0.0	0.0	0.0	12.147187315923818	0.0
27	0.0	0.0	0.0	12.342308069899863	0.0
28	0.0	0.0	0.0	12.547553013940703	0.0
29	0.0	0.0	0.0	12.75433192617318	0.0
30	0.0	0.0	0.0	12.986881504025133	0.0
31	0.0	0.0	0.0	13.218664097781268	0.0
32	0.0	0.0	0.0	13.445998183781661	0.0
33	0.0	0.0	0.0	13.668270174749656	0.0
34	0.0	0.0	0.0	13.922755497742	0.0
35	0.0	0.0	0.0	14.178774788925978	0.0
36	0.0	0.0	0.0	14.429885381896721	0.0
37	0.0	0.0	0.0	14.687131847634008	0.0
38	0.0	0.0	0.0	14.973063518554879	0.0
39	0.0	0.0	0.0	15.268659189083055	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGCGG	35	1.2108467E-7	45.0	2
CGTTTTT	7280	0.0	43.98008	1
CATACGA	80	0.0	42.1875	18
CGCTCGA	45	1.9261279E-8	40.0	41
CGATGAA	390	0.0	39.807693	19
GGCGATC	280	0.0	39.375	8
TTTCGCG	195	0.0	39.23077	1
CGTTTTC	290	0.0	38.793102	1
CTTCGCG	70	0.0	38.571426	1
ACACGCG	70	0.0	38.571426	36
GGATCGA	35	6.2441286E-6	38.571426	8
GGCGATA	70	0.0	38.571426	8
GGACCGA	310	0.0	38.46774	8
CTTGTCG	100	0.0	38.25	1
GTTTTTT	8460	0.0	38.031918	2
TTGGGAC	1735	0.0	37.73775	5
GACCGAT	305	0.0	37.62295	9
TTGGGAT	2870	0.0	37.552265	5
ACATACG	90	0.0	37.5	17
CCTCGCG	30	1.13938004E-4	37.499996	1
>>END_MODULE