##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2934080.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	568022
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.588304678339924	31.0	28.0	31.0	25.0	33.0
2	29.038322459341362	31.0	28.0	33.0	16.0	34.0
3	29.116727873216178	31.0	28.0	33.0	16.0	34.0
4	33.50566879451852	35.0	32.0	37.0	28.0	37.0
5	34.23213889602867	35.0	35.0	37.0	32.0	37.0
6	33.41441176574147	35.0	33.0	37.0	28.0	37.0
7	34.13858618152114	35.0	33.0	37.0	31.0	37.0
8	33.5647686181169	35.0	35.0	37.0	28.0	37.0
9	35.4176299509526	37.0	34.0	39.0	30.0	39.0
10	35.269017749312525	37.0	34.0	39.0	30.0	39.0
11	35.387720898134226	37.0	35.0	39.0	30.0	39.0
12	35.4910109115492	37.0	35.0	39.0	30.0	39.0
13	35.42520008027858	37.0	35.0	39.0	30.0	39.0
14	36.346322853692286	38.0	35.0	40.0	30.0	41.0
15	36.406468411434766	38.0	35.0	40.0	30.0	41.0
16	36.66114516691255	38.0	35.0	40.0	31.0	41.0
17	36.16562914816679	38.0	34.0	40.0	29.0	41.0
18	36.236059518821456	38.0	35.0	40.0	30.0	41.0
19	36.06469819830922	38.0	35.0	40.0	30.0	41.0
20	35.5285693159772	37.0	34.0	40.0	27.0	41.0
21	35.55000862642644	37.0	34.0	40.0	29.0	41.0
22	35.59760713493491	37.0	34.0	40.0	29.0	41.0
23	35.402612926964096	37.0	33.0	40.0	27.0	41.0
24	35.32628665791114	37.0	33.0	40.0	27.0	41.0
25	35.1816038815398	37.0	33.0	40.0	27.0	41.0
26	35.143885272049324	37.0	33.0	40.0	27.0	41.0
27	34.94627320772787	37.0	33.0	40.0	26.0	41.0
28	34.714877240670255	37.0	33.0	40.0	25.0	41.0
29	34.88395519891835	37.0	33.0	40.0	25.0	41.0
30	34.42528106305742	37.0	33.0	40.0	24.0	41.0
31	34.57052367689984	37.0	33.0	40.0	24.0	41.0
32	33.94882064427082	37.0	33.0	40.0	22.0	41.0
33	33.54101777748045	37.0	32.0	40.0	20.0	41.0
34	33.464971427163036	37.0	32.0	40.0	18.0	41.0
35	33.05207544778195	37.0	31.0	40.0	15.0	41.0
36	32.70188302565746	36.0	31.0	40.0	14.0	41.0
37	32.76829242529339	37.0	31.0	40.0	12.0	41.0
38	32.710181999992955	36.0	31.0	40.0	15.0	41.0
39	32.37936734844777	36.0	31.0	40.0	11.0	41.0
40	32.42117206727909	36.0	30.0	40.0	13.0	41.0
41	32.48518895394896	36.0	30.0	40.0	16.0	41.0
42	32.156277397706425	36.0	30.0	40.0	10.0	41.0
43	32.06508550725148	36.0	30.0	40.0	10.0	41.0
44	32.36965469647303	36.0	31.0	40.0	11.0	41.0
45	32.30781378186056	36.0	31.0	40.0	10.0	41.0
46	32.16455700659481	36.0	31.0	40.0	10.0	41.0
47	31.975337222854044	35.0	30.0	40.0	10.0	41.0
48	31.982314065300287	35.0	30.0	39.0	10.0	41.0
49	32.01331990662334	36.0	31.0	40.0	10.0	41.0
50	31.810877043494795	35.0	30.0	39.0	10.0	41.0
51	31.074224237793608	35.0	28.0	39.0	9.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	4.0
11	14.0
12	20.0
13	24.0
14	41.0
15	74.0
16	175.0
17	419.0
18	754.0
19	1750.0
20	3520.0
21	5961.0
22	8566.0
23	10886.0
24	12775.0
25	14920.0
26	15711.0
27	15526.0
28	15593.0
29	16555.0
30	19482.0
31	23189.0
32	28000.0
33	34325.0
34	43054.0
35	49189.0
36	55824.0
37	67992.0
38	80852.0
39	42821.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	28.04521655851358	3.8686881846125676	41.548918879902544	26.537176376971317
2	25.49883631267803	17.391579903595282	40.70018414779709	16.409399635929596
3	15.129343581762678	17.371686307924694	49.68997679667337	17.80899331363926
4	14.695381516913079	4.078363161990205	59.26407779980353	21.96217752129319
5	25.659745573234837	4.339620648496009	48.51695885018538	21.483674928083772
6	17.483829851660676	15.795691011967847	51.382516874346415	15.337962262025062
7	65.93300963695069	1.9705222685036847	28.201724581090172	3.894743513455465
8	66.03987169511039	12.248117150392062	17.129442169493437	4.582568985004102
9	61.65377397354328	4.063575002376669	17.25390917957403	17.028741844506023
10	39.3361524729676	22.418497875082302	24.33321244599681	13.91213720595329
11	29.227213030481213	19.836555626366582	32.3147694983645	18.621461844787703
12	19.83426698261687	17.08067645267261	36.41249810746767	26.67255845724285
13	25.044100404561796	17.972015168426577	39.139857259049826	17.844027167961805
14	16.57770297629317	28.011767149863914	35.172933442718765	20.237596431124146
15	14.56387252606413	20.90042287094514	39.59265662245476	24.943047980535965
16	16.615201523884636	22.511804120262948	33.59007221551278	27.282922140339632
17	17.13032241708948	28.650298756034097	34.76009732017422	19.459281506702204
18	16.717662344064138	22.414096637102084	34.25025791254564	26.617983106288136
19	17.38665051705744	24.292192908021168	31.77341722679755	26.547739348123837
20	24.9578361401495	23.30138621391425	34.95516018745753	16.785617458478722
21	20.00538711528779	30.298298305347327	32.467404431518496	17.228910147846385
22	19.147497808183488	20.34234589505336	39.24002943547961	21.270126861283543
23	24.433032523388178	23.330082285545277	33.664365112618874	18.572520078447667
24	19.55100330620997	27.037685160081825	32.75260465263669	20.65870688107151
25	17.786282925661332	32.10069328300664	30.323649436113392	19.789374355218637
26	18.08820785110436	22.4746576717099	37.44414829003102	21.992986187154724
27	25.130012569935673	22.943829640401255	31.196326902831228	20.72983088683185
28	17.361123336772167	28.13517786282926	33.16385632950836	21.33984247089021
29	19.031833274063327	19.937432000873205	37.393622078018105	23.63711264704536
30	18.08750365302752	28.11862920802363	34.22789962360613	19.565967515342717
31	21.097422282939746	21.181750002640744	29.7171588424392	28.00366887198031
32	25.75199552130023	23.141181151434274	29.878244152515222	21.228579174750273
33	24.343951466668546	21.369242740598075	31.083127061979994	23.203678730753385
34	19.195911425965896	23.03379094471693	28.96120220695677	28.809095422360404
35	23.41053691582368	24.619468964230258	30.59969508223273	21.370299037713327
36	19.31175200960526	28.943245155997477	29.997957825577178	21.74704500882008
37	17.865329159786064	24.13568488544458	32.670037428127785	25.328948526641575
38	19.922467791740463	23.75964311241466	29.824020900598917	26.493868195245955
39	19.70064539753742	26.923957170672967	29.153800380971163	24.221597050818456
40	18.261088478967363	25.094098468017084	31.743136709493648	24.901676343521906
41	17.989796169866658	25.11170341993796	30.168725859209676	26.729774550985702
42	21.706729668921273	25.423663167975892	31.407058177324114	21.46254898577872
43	20.263475710447835	26.588406787061064	29.959050881832045	23.18906662065906
44	20.35343701476351	23.22762146536578	33.06650094538592	23.352440574484792
45	21.5525102900944	22.744365535137725	29.497449042466666	26.205675132301216
46	23.155441162490185	26.546859100527797	28.014759991690465	22.282939745291554
47	17.206551858906874	28.115108217639456	34.221209741876194	20.457130181577472
48	19.499596846601012	24.669290978166337	31.327659844160966	24.503452331071685
49	20.178971941227626	23.494512536486262	34.0687508582414	22.257764664044704
50	19.399600719690437	24.73442930027358	33.17318695402643	22.692783026009554
51	18.73853477506153	22.061469450126932	33.00329916798997	26.19669660682157
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	389.0
1	513.5
2	638.0
3	8820.5
4	17003.0
5	11783.5
6	6564.0
7	6302.5
8	6041.0
9	6022.5
10	6004.0
11	6004.0
12	6004.0
13	5735.5
14	5467.0
15	5131.5
16	4796.0
17	4513.0
18	4230.0
19	4055.5
20	3881.0
21	3893.5
22	3906.0
23	4020.0
24	4134.0
25	4398.0
26	5180.0
27	5698.0
28	6212.5
29	6727.0
30	7840.5
31	8954.0
32	10009.0
33	11064.0
34	12642.0
35	14220.0
36	14848.5
37	15477.0
38	16417.5
39	17358.0
40	18793.0
41	20228.0
42	21185.5
43	22143.0
44	24709.5
45	27276.0
46	48148.5
47	69021.0
48	56807.5
49	44594.0
50	42923.5
51	41253.0
52	38684.5
53	36116.0
54	34848.5
55	33581.0
56	31691.5
57	29802.0
58	27355.0
59	24908.0
60	22164.5
61	19421.0
62	17083.5
63	14746.0
64	12913.0
65	11080.0
66	9367.5
67	7655.0
68	6282.0
69	4909.0
70	4148.0
71	3387.0
72	2807.0
73	2227.0
74	1733.0
75	951.5
76	664.0
77	484.0
78	304.0
79	231.5
80	159.0
81	111.0
82	63.0
83	43.0
84	23.0
85	13.5
86	4.0
87	2.0
88	0.0
89	0.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	568022.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.09609707149506
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.23239703324231	33.07671788241361
2	11.788459367816955	11.10380853420165
3	6.628308339190883	9.36502358887002
4	4.231887694575585	7.972215746375885
5	2.620028072239849	6.169654821012498
6	1.5647961898966734	4.42174759538876
7	0.9815732176397424	3.235978727851873
8	0.560988009108932	2.113627658635121
9	0.358250529183222	1.5184981528494745
>10	0.9585524949255827	7.061357434818333
>50	0.04288209612099662	1.4237140841714089
>100	0.023528229472345286	2.264967165349636
>500	0.0030359005770768067	0.9609980626595901
>1k	0.003794875721346008	3.095675552041428
>5k	7.589751442692017E-4	2.3872142687268263
>10k+	7.589751442692017E-4	3.828800724633884
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTC	10883	1.915946917548968	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10540	1.8555619324603625	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCG	7231	1.2730140733985655	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGC	6126	1.0784793546728824	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT	3049	0.5367749840675186	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTGCTCATT	2647	0.4660030773455958	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTC	1944	0.3422402653418353	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT	1550	0.2728767547735827	No Hit
GCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTGC	1549	0.27270070525437395	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCT	1483	0.2610814369865956	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTT	1386	0.24400463362334557	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCT	1319	0.23220931583635843	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGCTCATTCGTA	1292	0.22745597881772187	No Hit
CGTTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTC	1102	0.19400657016805686	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCC	868	0.1528109826732063	No Hit
CGCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCTG	785	0.138198872578879	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGT	706	0.12429096056138672	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCTGCTCATTCGT	659	0.11601663315857484	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTC	614	0.10809440479418052	No Hit
CGTCTGTCTCTTATACACATCTGACGCCTGCTCATTCGTATGCCGTCTTCT	608	0.10703810767892794	No Hit
GAATGATACGGCGACCACCGATGAATGATACGGCGACCCCCGATCGAATGC	575	0.10122847354503875	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05985683653097943	0.0
2	0.0	0.0	0.0	0.49593149561108546	0.0
3	0.0	0.0	0.0	0.7415205749073099	0.0
4	0.0	0.0	0.0	1.6351479344109912	0.0
5	0.0	0.0	0.0	4.385041424451869	0.0
6	0.0	0.0	0.0	5.512462545464788	0.0
7	0.0	0.0	0.0	6.575625591966508	0.0
8	0.0	0.0	0.0	7.622063934143396	0.0
9	0.0	0.0	0.0	8.219752051857146	0.0
10	0.0	0.0	0.0	10.171613071324701	0.0
11	0.0	0.0	0.0	10.82510888662763	0.0
12	0.0	0.0	0.0	12.588244821503393	0.0
13	0.0	0.0	0.0	12.899324321945278	0.0
14	0.0	0.0	0.0	13.110583744995793	0.0
15	1.7604951920876304E-4	0.0	0.0	13.485041072352832	0.0
16	1.7604951920876304E-4	0.0	0.0	13.766720303086853	0.0
17	1.7604951920876304E-4	0.0	0.0	13.998577519884794	0.0
18	1.7604951920876304E-4	0.0	0.0	14.18254926745795	0.0
19	1.7604951920876304E-4	0.0	0.0	14.943611338997433	0.0
20	1.7604951920876304E-4	0.0	0.0	15.16754632743098	0.0
21	1.7604951920876304E-4	0.0	0.0	15.359440303368531	0.0
22	1.7604951920876304E-4	0.0	0.0	15.592705916320142	0.0
23	1.7604951920876304E-4	0.0	0.0	15.797275457640724	0.0
24	3.520990384175261E-4	0.0	0.0	15.987408938386189	0.0
25	3.520990384175261E-4	0.0	0.0	16.150078694135086	0.0
26	3.520990384175261E-4	0.0	0.0	16.311516103249524	0.0
27	3.520990384175261E-4	0.0	0.0	16.50675502005204	0.0
28	3.520990384175261E-4	0.0	0.0	16.67629070705008	0.0
29	3.520990384175261E-4	0.0	0.0	16.83825626472214	0.0
30	3.520990384175261E-4	0.0	0.0	17.016418378161408	0.0
31	3.520990384175261E-4	0.0	0.0	17.198629630542477	0.0
32	3.520990384175261E-4	0.0	0.0	17.381545081000382	0.0
33	3.520990384175261E-4	0.0	0.0	17.551784966075257	0.0
34	3.520990384175261E-4	0.0	0.0	17.74684783335857	0.0
35	3.520990384175261E-4	0.0	0.0	17.941910700641877	0.0
36	3.520990384175261E-4	0.0	0.0	18.16038815397995	0.0
37	3.520990384175261E-4	0.0	0.0	18.35967620972427	0.0
38	3.520990384175261E-4	0.0	0.0	18.60051195200186	0.0
39	3.520990384175261E-4	0.0	0.0	18.829904475530878	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACGGGA	65	0.0	45.000004	4
ATCGTAA	20	7.0302683E-4	45.000004	14
CGTACAC	20	7.0302683E-4	45.000004	34
CGTAAGC	80	0.0	45.000004	43
ATACGTC	30	2.163555E-6	45.000004	28
CAATCGA	60	0.0	45.000004	41
TACGTCG	20	7.0302683E-4	45.000004	29
AGCGCGA	25	3.888306E-5	45.0	24
TGTCGCG	25	3.888306E-5	45.0	1
CGATCGA	230	0.0	44.021736	41
CGTTTTT	5975	0.0	43.832638	1
CCGATCA	155	0.0	43.548386	40
TACGGCT	790	0.0	42.72152	7
ATGAATG	3230	0.0	42.701237	21
ACCGAAT	95	0.0	42.63158	39
GATGAAT	3275	0.0	42.251907	20
TGAATGA	3205	0.0	42.19189	22
CGATGAA	3050	0.0	42.122948	19
ACCCGTC	75	0.0	42.0	39
CGCCCAC	70	0.0	41.785717	34
>>END_MODULE