SRR2934079.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2934079.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	400294
Sequences flagged as poor quality	0
Sequence length	51
%GC	46

Per base quality graph

Per Sequence quality graph

Per sequence GC content graph

N content graph

Sequence length distribution

Duplication level graph

Sequence	Count	Percentage	Possible Source
CCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1621	0.4049523600153887	RNA PCR Primer, Index 10 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1608	0.401704747010947	RNA PCR Primer, Index 35 (95% over 23bp)
GCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGC	1573	0.39296117353744997	RNA PCR Primer, Index 35 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCTGCT	1459	0.3644821056523455	TruSeq Adapter, Index 10 (96% over 25bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGC	1268	0.3167671761255477	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCG	1088	0.27180022683327754	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTC	788	0.19685531134616055	RNA PCR Primer, Index 10 (95% over 21bp)
TCTCCGGGGTAGAAATAATGAACTTCACTGGTCATGATAATGCATACCTAA	536	0.13390158233698232	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCTCTTCTCT	492	0.12290966139887183	No Hit
TTCCTGTCTCTTATACACATCTGACGCCTCTTCTCTCGTATGCCGTCTTCT	443	0.11066865853597606	RNA PCR Primer, Index 35 (95% over 21bp)

Adapter graph

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGCGG	25	3.8862338E-5	45.000004	2
TCGACGG	35	1.2092823E-7	45.000004	2
GCCCGAC	35	1.2092823E-7	45.000004	9
TTTAGCG	20	7.0277706E-4	45.0	1
CGGGTCG	20	7.0277706E-4	45.0	6
ATCGTTC	20	7.0277706E-4	45.0	37
GTAGCGG	20	7.0277706E-4	45.0	2
TACGATA	20	7.0277706E-4	45.0	17
TCCGTTG	45	3.8380676E-10	45.0	1
TAAGGTT	20	7.0277706E-4	45.0	12
TTCGATG	20	7.0277706E-4	45.0	1
GACTAGA	20	7.0277706E-4	45.0	12
TTCAGCG	30	2.1619435E-6	44.999996	1
CGGGTGC	85	0.0	42.35294	6
TCTCCCG	55	6.002665E-11	40.909092	1
GCCGTTG	55	6.002665E-11	40.909092	1
TTGGGTC	375	0.0	40.8	5
GTTCGCG	50	1.0786607E-9	40.500004	1
GGCACCG	145	0.0	40.344826	8
GCACCGA	140	0.0	40.178574	9